AB229890

Rat Fractalkine ELISA Kit

Recombinant
SimpleStep
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Rat Fractalkine ELISA Kit is a single-wash 90-min Simplestep used to quantify Rat Fractalkine with a sensitivity of 9.8 pg/ml. The assay uses a simple mix-wash-read protocol with just one incubation and one wash step.

- Colorimetric Sandwich ELISA - 450 nm readout : works on any standard plate reader
- Design your own immunoassay: we also offer the conjugation-ready antibody pair

View Alternative Names

Acc1, Fkn, Scyd1, Cx3cl1, Fractalkine, C-X3-C motif chemokine 1, CX3C membrane-anchored chemokine, Neurotactin, Small-inducible cytokine D1

4 Images

Sandwich ELISA - Rat Fractalkine ELISA Kit (AB229890)

sELISA

Supplier Data

Sandwich ELISA - Rat Fractalkine ELISA Kit (AB229890)

Interpolated concentrations of spike Fractalkine in rat cell culture media sample.

sELISA

Supplier Data

Sandwich ELISA - Rat Fractalkine ELISA Kit (AB229890)

Interpolated concentrations of native Fractalkine in rat serum, plasma (EDTA), plasma (heparin) and plasma (citrate) samples.

The concentrations of Fractalkine were measured in duplicates, interpolated from the Fractalkine standard curves and corrected for sample dilution. Undiluted samples are as follows : serum 100%, plasma (EDTA) 100%, plasma (heparin) 50% and plasma (citrate) 100%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean Fractalkine concentration was determined to be 151 ng/mL in serum, 118 ng/mL in plasma (EDTA) 250 ng/mL in plasma (heparin), and 93 ng/mL in plasma (citrate).

sELISA

Supplier Data

Sandwich ELISA - Rat Fractalkine ELISA Kit (AB229890)

Example of rat Fractalkine standard curve.

The Fractalkine standard curve was prepared as described in Section 10. Background-subtracted data values (mean +/- SD) are graphed.

sELISA

Supplier Data

Sandwich ELISA - Rat Fractalkine ELISA Kit (AB229890)

Interpolated concentrations of spike Fractalkine in rat serum, plasma (EDTA), plasma (heparin) and plasma (citrate) samples.

Key facts

Detection method

Colorimetric

Sample types

Heparin Plasma, Citrate plasma, Cell culture supernatant, Serum, EDTA Plasma

Reacts with

Rat

Assay type

Sandwich (quantitative)

Sensitivity

= 9.8 pg/mL

Range

16 - 1000 pg/mL

Assay time

1h 30m

Assay Platform

Pre-coated microplate (12 x 8 well strips)

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }

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Product details

Rat Fractalkine ELISA Kit (ab229890) is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of Fractalkine protein in cell culture supernatant, cit plasma, edta plasma, hep plasma, and serum. It uses our proprietary SimpleStep ELISA® technology. Quantitate Rat Fractalkine with 9.8 pg/ml sensitivity.

SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:

- Single-wash protocol reduces assay time to 90 minutes or less
- High sensitivity, specificity and reproducibility from superior antibodies
- Fully validated in biological samples
- 96-wells plate breakable into 12 x 8 wells strips

A 384-well SimpleStep ELISA® microplate (ab203359) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.

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Precision

[ { "reproducibilityType": "Inter", "sample": "Rat Serum", "replicates": 3, "mean": null, "standardDeviation": null, "coefficientOfVariability": "4" }, { "reproducibilityType": "Intra", "sample": "Rat Serum", "replicates": 5, "mean": null, "standardDeviation": null, "coefficientOfVariability": "7.2" } ]

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Recovery

[ { "sample": "Serum", "range": "93 - 111 %", "average": "= 102" }, { "sample": "EDTA Plasma", "range": "89 - 101 %", "average": "= 95" }, { "sample": "Heparin Plasma", "range": "104 - 116 %", "average": "= 112" }, { "sample": "Citrate plasma", "range": "79 - 84 %", "average": "= 81" }, { "sample": "Cell culture media", "range": "111 - 113 %", "average": "= 112" } ]

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What's included?

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Properties and storage information

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

+4°C

Storage information

+4°C

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

CX3CL1 also known as fractalkine is a chemokine protein characterized mechanically by its dual function as both a chemoattractant and adhesion molecule. It exhibits a unique membrane-bound structure and can exist in a soluble form after proteolytic cleavage. The mass of CX3CL1 is approximately 42-45 kDa. This protein is highly expressed in endothelial cells neurons and tissues with substantial vascularization. Considerable interest surrounds the potential of CX3CL1 assessment in various studies often involving applications like fractalkine ELISA or assays on mouse models.

Biological function summary

CX3CL1 is involved in the immune system and nervous system functions influencing leukocyte adhesion and migration. It is not known to be part of a large multiprotein complex but directly interacts with its fractalkine receptor CX3CR1 on the surface of immune cells. This interaction regulates communication between neurons and microglia which highlights its role in neuroinflammation. CX3CL1's expression and function suggest a significant influence over inflammatory responses and homeostatic balance within these systems.

Pathways

CX3CL1 plays a role in the MAPK and PI3K-Akt pathways vital for cell survival proliferation and migration. Within these pathways CX3CL1 influences and interacts with several proteins thereby modulating inflammatory responses and cellular migration. These pathways are essential for immune system regulation where CX3CL1 acts as a mediator in signal transduction processes that connect immune responses to cellular activities such as proliferation and survival.

CX3CL1 has associations with neurodegenerative disorders like Alzheimer's disease and inflammatory diseases such as rheumatoid arthritis. Alterations in CX3CL1 expression or its fractalkine receptor function can influence disease progression by affecting how immune cells communicate and migrate to inflamed or damaged tissues. CX3CR1 the receptor for CX3CL1 represents an important link in these connections driving research efforts to explore therapies targeting these interactions to alleviate disease symptoms.

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Product protocols

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Target data

Chemokine that acts as a ligand for both CX3CR1 and integrins ITGAV : ITGB3 and ITGA4 : ITGB1. The CX3CR1-CX3CL1 signaling exerts distinct functions in different tissue compartments, such as immune response, inflammation, cell adhesion and chemotaxis. Regulates leukocyte adhesion and migration processes at the endothelium. Can activate integrins in both a CX3CR1-dependent and CX3CR1-independent manner. In the presence of CX3CR1, activates integrins by binding to the classical ligand-binding site (site 1) in integrins. In the absence of CX3CR1, binds to a second site (site 2) in integrins which is distinct from site 1 and enhances the binding of other integrin ligands to site 1.. Processed fractalkine. The soluble form is chemotactic for T-cells and monocytes, but not for neutrophils.. Fractalkine. The membrane-bound form promotes adhesion of those leukocytes to endothelial cells.

See full target information Cx3cl1

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websiteProtocolBooklet

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Rat Fractalkine ELISA Kit

Sandwich ELISA - Rat Fractalkine ELISA Kit (AB229890)

Sandwich ELISA - Rat Fractalkine ELISA Kit (AB229890)

Sandwich ELISA - Rat Fractalkine ELISA Kit (AB229890)

Sandwich ELISA - Rat Fractalkine ELISA Kit (AB229890)

Key facts

Detection method

Sample types

Reacts with

Assay type

Sensitivity

Range

Assay time

Assay Platform

Reactivity data

Product details

Precision

Recovery

What's included?

Properties and storage information

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

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