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AB100761

Rat Fractalkine ELISA Kit (CX3CL1)

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(2 Publications)

Rat Fractalkine ELISA Kit (CX3CL1) is a Sandwich (quantitative) ELISA for the measurement of Rat Fractalkine (CX3CL1) in Rat in Cell/Tissue Extracts samples.

View Alternative Names

Acc1, Fkn, Scyd1, Cx3cl1, Fractalkine, C-X3-C motif chemokine 1, CX3C membrane-anchored chemokine, Neurotactin, Small-inducible cytokine D1

1 Images
Sandwich ELISA - Rat Fractalkine ELISA Kit (CX3CL1) (AB100761)
  • sELISA

Supplier Data

Sandwich ELISA - Rat Fractalkine ELISA Kit (CX3CL1) (AB100761)

Representative Standard Curve using ab100761

Key facts

Detection method

Colorimetric

Sample types

Cell culture extracts, Tissue Extracts

Reacts with

Rat

Assay type

Sandwich (quantitative)

Sensitivity

< 5 pg/mL

Range

2.74 - 2000 pg/mL

Assay Platform

Microplate

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Product details

Abcam's Fractalkine Rat ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of Rat Fractalkine in cell lysates and tissue lysates.

This assay employs an antibody specific for Rat Fractalkine coated on a 96- well plate. Standards and samples are pipetted into the wells and Fractalkine present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-Rat Fractalkine antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of Fractalkine bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.

The standard is affinity purified.

REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.

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Recovery

[ { "sample": "Cell culture extracts", "range": "84 - 104 %", "average": "= 93.54" }, { "sample": "Tissue Extracts", "range": "83 - 103 %", "average": "= 92.87" } ]
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What's included?

{ "values": { "1x96Tests": { "sellingSize": "1 x 96 Tests", "publicAssetCode":"ab100761-1x96Tests", "assetComponentDetails": [ { "size":"1 x 25 mL", "name":"20X Wash Buffer", "number":"AB100761-CMP02", "productcode":"" }, { "size":"1 x 5 mL", "name":"2X Cell Lysis Buffer", "number":"AB100761-CMP07", "productcode":"" }, { "size":"1 x 8 mL", "name":"Stop Solution", "number":"AB100761-CMP05", "productcode":"" }, { "size":"1 x 10 mL", "name":"5X Sample Diluent Buffer", "number":"AB100761-CMP01", "productcode":"" }, { "size":"2 x 1 Vial", "name":"Recombinant Rat Fractalkine Standard (lyophilized)", "number":"AB100761-CMP09", "productcode":"" }, { "size":"1 x 12 mL", "name":"TMB One-Step Substrate Reagent", "number":"AB100761-CMP06", "productcode":"" }, { "size":"1 x 200 µL", "name":"240X HRP-Streptavidin Concentrate", "number":"AB100761-CMP03", "productcode":"" }, { "size":"1 x 1 Unit", "name":"Fractalkine Microplate (12 x 8 wells)", "number":"AB100761-CMP08", "productcode":"" }, { "size":"2 x 1 Vial", "name":"Biotinylated anti-Rat Fractalkine", "number":"AB100761-CMP10", "productcode":"" }, { "size":"1 x 15 mL", "name":"5X Assay Diluent", "number":"AB100761-CMP04", "productcode":"" } ] } } }
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Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
-20°C
Appropriate long-term storage conditions
-20°C
Storage information
-20°C
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

CX3CL1 also known as fractalkine is a chemokine protein characterized mechanically by its dual function as both a chemoattractant and adhesion molecule. It exhibits a unique membrane-bound structure and can exist in a soluble form after proteolytic cleavage. The mass of CX3CL1 is approximately 42-45 kDa. This protein is highly expressed in endothelial cells neurons and tissues with substantial vascularization. Considerable interest surrounds the potential of CX3CL1 assessment in various studies often involving applications like fractalkine ELISA or assays on mouse models.
Biological function summary

CX3CL1 is involved in the immune system and nervous system functions influencing leukocyte adhesion and migration. It is not known to be part of a large multiprotein complex but directly interacts with its fractalkine receptor CX3CR1 on the surface of immune cells. This interaction regulates communication between neurons and microglia which highlights its role in neuroinflammation. CX3CL1's expression and function suggest a significant influence over inflammatory responses and homeostatic balance within these systems.

Pathways

CX3CL1 plays a role in the MAPK and PI3K-Akt pathways vital for cell survival proliferation and migration. Within these pathways CX3CL1 influences and interacts with several proteins thereby modulating inflammatory responses and cellular migration. These pathways are essential for immune system regulation where CX3CL1 acts as a mediator in signal transduction processes that connect immune responses to cellular activities such as proliferation and survival.

CX3CL1 has associations with neurodegenerative disorders like Alzheimer's disease and inflammatory diseases such as rheumatoid arthritis. Alterations in CX3CL1 expression or its fractalkine receptor function can influence disease progression by affecting how immune cells communicate and migrate to inflamed or damaged tissues. CX3CR1 the receptor for CX3CL1 represents an important link in these connections driving research efforts to explore therapies targeting these interactions to alleviate disease symptoms.
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Product protocols

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Target data

Chemokine that acts as a ligand for both CX3CR1 and integrins ITGAV : ITGB3 and ITGA4 : ITGB1. The CX3CR1-CX3CL1 signaling exerts distinct functions in different tissue compartments, such as immune response, inflammation, cell adhesion and chemotaxis. Regulates leukocyte adhesion and migration processes at the endothelium. Can activate integrins in both a CX3CR1-dependent and CX3CR1-independent manner. In the presence of CX3CR1, activates integrins by binding to the classical ligand-binding site (site 1) in integrins. In the absence of CX3CR1, binds to a second site (site 2) in integrins which is distinct from site 1 and enhances the binding of other integrin ligands to site 1.. Processed fractalkine. The soluble form is chemotactic for T-cells and monocytes, but not for neutrophils.. Fractalkine. The membrane-bound form promotes adhesion of those leukocytes to endothelial cells.
See full target information Cx3cl1
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Publications (2)

Recent publications for all applications. Explore the full list and refine your search

The international journal of neuropsychopharmacology 28: PubMed39985181

2025

Synergistic effects of memantine and alpha7 nicotinic acetylcholine receptor agonist PHA-543613 to improve memory of aged rats.

Applications

Unspecified application

Species

Unspecified reactive species

Nóra Bruszt,Zsolt Kristóf Bali,Lili Veronika Nagy,Kornélia Bodó,Péter Engelmann,István Hernádi

Mediators of inflammation 2017:9734837 PubMed28634422

2017

Lipopolysaccharide-Binding Protein Downregulates Fractalkine through Activation of p38 MAPK and NF-B.

Applications

ELISA

Species

Rat

Xia Huang,Yi Zeng,Yujie Jiang,Yueqiu Qin,Weigui Luo,Shulin Xiang,Suren R Sooranna,Liao Pinhu
View all publications
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