AB246546

Rat FSTL1 ELISA Kit

Recombinant
SimpleStep
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(1 Publication)

Rat FSTL1 ELISA Kit is a single-wash 90-min Simplestep used to quantify Rat FSTL1 with a sensitivity of 24 pg/ml. The assay uses a simple mix-wash-read protocol with just one incubation and one wash step.

- Colorimetric Sandwich ELISA - 450 nm readout : works on any standard plate reader
- Design your own immunoassay: we also offer the conjugation-ready antibody pair

View Alternative Names

Frp, Fstl1, Follistatin-related protein 1, Follistatin-like protein 1

3 Images

Sandwich ELISA - Rat FSTL1 ELISA Kit (AB246546)

sELISA

Supplier Data

Sandwich ELISA - Rat FSTL1 ELISA Kit (AB246546)

Interpolated concentrations of native FSTL1 in rat serum and plasmas samples.

The concentrations of FSTL1 were measured in duplicates, interpolated from the FSTL1 standard curves and corrected for sample dilution. Undiluted samples are as follows : serum 5%, plasma (EDTA) 5%, plasma (citrate) 2.5%, plasma (heparin) 2.5% and urine 50%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean FSTL1 concentration was determined to be 36,218 pg/mL in serum, 72,246 pg/mL in plasma (EDTA), 58,994 pg/mL in plasma (citrate), and 75,205 pg/mL in plasma (heparin).

sELISA

Supplier Data

Sandwich ELISA - Rat FSTL1 ELISA Kit (AB246546)

Interpolated concentrations of native FSTL1 in rat urine samples.

The concentrations of FSTL1 were measured in duplicates, interpolated from the FSTL1 standard curves and corrected for sample dilution. Undiluted samples are as follows : urine 50%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean FSTL1 concentration was determined to be 4,662 pg/mL in urine.

sELISA

Supplier Data

Sandwich ELISA - Rat FSTL1 ELISA Kit (AB246546)

Example of rat FSTL1 standard curve in Sample Diluent NS.

The FSTL1 standard curve was prepared as described in Section 10. Raw data values are shown in the table. Background-subtracted data values (mean +/- SD) are graphed.

Key facts

Detection method

Colorimetric

Sample types

Urine, Heparin Plasma, Citrate plasma, Serum, EDTA Plasma

Reacts with

Rat

Assay type

Sandwich (quantitative)

Sensitivity

= 24 pg/mL

Range

78 - 5000 pg/mL

Assay Platform

Pre-coated microplate (12 x 8 well strips)

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }

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Product details

Rat FSTL1 ELISA Kit (ab246546) is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of FSTL1 protein in cit plasma, edta plasma, hep plasma, serum, and urine. It uses our proprietary SimpleStep ELISA® technology. Quantitate Rat FSTL1 with 24 pg/ml sensitivity.

SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:

- Single-wash protocol reduces assay time to 90 minutes or less
- High sensitivity, specificity and reproducibility from superior antibodies
- Fully validated in biological samples
- 96-wells plate breakable into 12 x 8 wells strips

A 384-well SimpleStep ELISA® microplate (ab203359) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.

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Precision

[ { "reproducibilityType": "Inter", "sample": "Serum", "replicates": 3, "mean": null, "standardDeviation": null, "coefficientOfVariability": "5.2" }, { "reproducibilityType": "Intra", "sample": "Serum", "replicates": 8, "mean": null, "standardDeviation": null, "coefficientOfVariability": "3.6" } ]

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Recovery

[ { "sample": "Serum", "range": "99 - 103 %", "average": "= 101" }, { "sample": "EDTA Plasma", "range": "91 - 105 %", "average": "= 96" }, { "sample": "Urine", "range": "107 - 113 %", "average": "= 110" }, { "sample": "Heparin Plasma", "range": "84 - 98 %", "average": "= 92" }, { "sample": "Citrate plasma", "range": "90 - 107 %", "average": "= 100" } ]

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What's included?

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Properties and storage information

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

+4°C

Storage information

+4°C

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

FSTL1 also known as FRP is a protein that plays an important role in various cellular functions. The protein is a glycoprotein with a molecular mass of approximately 32 kDa and is expressed in a variety of tissues including heart lung and placenta. FSTL1 contains several domains that facilitate its interaction with other proteins and cellular components. It is located both intracellularly and secreted in the extracellular matrix contributing to its role in cell signaling and structural integrity.

Biological function summary

FSTL1 serves as a modulator in inflammatory and developmental processes. It is a part of a complex that regulates immune responses and cell growth. The protein has been shown to affect the activity of multiple growth factors including Bone Morphogenetic Proteins (BMPs) revealing its regulatory capacity in tissue development and repair. FSTL1 can influence cell differentiation proliferation and survival highlighting its significance in maintaining cellular homeostasis.

Pathways

FSTL1 has important roles in the regulation of the BMP signaling and Wnt pathways. In the BMP pathway FSTL1 interacts with other signaling molecules to modulate pathway intensity and cellular outcomes. The protein links with BMP proteins to influence processes like bone formation and tissue repair. In the Wnt pathway FSTL1 can modulate key interactions that affect cellular proliferation and development. Through these pathways FSTL1 also relates to proteins such as TGF-beta which participate in various cellular processes.

FSTL1 has relevance in conditions such as rheumatoid arthritis and pulmonary hypertension. The protein shows increased expression in inflammatory environments indicating its involvement in rheumatoid arthritis where it engages with immune response pathways. The connection with pulmonary hypertension also positions FSTL1 as a significant factor in cardiovascular pathologies. In both conditions FSTL1 interacts with cytokines and other immune-related proteins influencing disease progression and response to treatment.

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Product protocols

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Target data

Secreted glycoprotein that is involved in various physiological processes, such as angiogenesis, regulation of the immune response, cell proliferation and differentiation (By similarity). Plays a role in the development of the central nervous system, skeletal system, lungs, and ureter. Promotes endothelial cell survival, migration and differentiation into network structures in an AKT-dependent manner. Also promotes survival of cardiac myocytes (By similarity). Initiates various signaling cascades by activating different receptors on the cell surface such as DIP2A, TLR4 or BMP receptors (By similarity).

See full target information Fstl1

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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Biomeditsinskaia khimiia 66:250-256 PubMed32588831

2020

[Study of the influence of etoxidol on expression of follistatin-like protein-1 (FSTL-1) in myocardium after experimental infarction in rats].

Applications

Unspecified application

Species

Unspecified reactive species

E V Parfenova,E S Zubkova,M A Boldyreva,Z I Tsokolaeva,Yu V Olefir,B K Romanov,A B Prokofiev,V G Kukes,O A Goroshko,T V Aleksandrova,A A Gazdanova,O K Parfenova,N G Sidorov,A D Andreev

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Rat FSTL1 ELISA Kit

Sandwich ELISA - Rat FSTL1 ELISA Kit (AB246546)

Sandwich ELISA - Rat FSTL1 ELISA Kit (AB246546)

Sandwich ELISA - Rat FSTL1 ELISA Kit (AB246546)

Key facts

Detection method

Sample types

Reacts with

Assay type

Sensitivity

Range

Assay Platform

Reactivity data

Product details

Precision

Recovery

What's included?

Properties and storage information

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (1)

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