Products
Sample Prep & Detection Kits
Conjugation kitsPurification kitsSample preparation kitsChromogen kitsIHC kitsChIP kitsAccessory Reagents & Controls
Accessory reagents & controlsBiochemicals
BiochemicalsProteins and Peptides
Proteins and peptidesOur latest ELISA kit: Human Tau (phospho T217) - Intracellular
Highly sensitive kit offering the most promising biomarkers for Alzheimer’s disease diagnostics. Learn about all product ranges with our product overviews.
Resources
Featured events
Make new connections at our global events.
Support
Our programs
New Lab Program
Get a head start with our exclusive new lab discount. Enjoy 20% off and free shipping for three months.
New Biotech Program
Just starting out? Get 15% off and free shipping to your lab for six months.
Product promise
Peace of mind that all products perform as stated.
Product reviews
Leave reviews, get rewarded and help your community.
Trial program
Try untested species and applications to earn money off your next order.
Rat FXII ELISA kit (total FXII antigen) is a Quantitative ELISA kit for the measurement of Rat FXII (total FXII antigen) in Rat in Citrate plasma, EDTA Plasma samples.
Alternative names=Coagulation factor XII, Hageman factor, HAF, F12
- Detection method
Colorimetric
- Sample types
Citrate plasma, EDTA Plasma
- Assay type
Quantitative
- Reactive species
Rat
- Range
0.02 - 10 ng/mL
Reactivity data
| Application | Reactivity | Dilution info | Notes |
|---|---|---|---|
Application ELISA | Reactivity Reacts | Dilution info - | Notes - |
Images
Target data
Function
Factor XII is a serum glycoprotein that participates in the initiation of blood coagulation, fibrinolysis, and the generation of bradykinin and angiotensin. Prekallikrein is cleaved by factor XII to form kallikrein, which then cleaves factor XII first to alpha-factor XIIa and then trypsin cleaves it to beta-factor XIIa. Alpha-factor XIIa activates factor XI to factor XIa (By similarity).
What's included?
Recommended products
Rat FXII ELISA kit (total FXII antigen) is a Quantitative ELISA kit for the measurement of Rat FXII (total FXII antigen) in Rat in Citrate plasma, EDTA Plasma samples.
Alternative names=Coagulation factor XII, Hageman factor, HAF, F12
Key facts
- Detection method
Colorimetric
- Sample types
Citrate plasma, EDTA Plasma
- Assay type
Quantitative
- Reactive species
Rat
- Range
0.02 - 10 ng/mL
- Assay Platform
Microplate
Storage
- Shipped at conditions
Blue Ice
- Appropriate long-term storage conditions
+4°C
- Storage information
+4°C
Notes
Rat FXII ELISA kit (total FXII antigen) (ab272782) is intended for the quantitative determination of total Factor XII antigen in rat plasma.
Rat Factor XII will bind to the affinity purified capture antibody coated on the microtiter plate. Factor XII and XIIa will react with the antibody on the plate. After appropriate washing steps, biotin labeled anti‑rat Factor XII primary antibody binds to the captured protein. Excess primary antibody is washed away and bound antibody is reacted with peroxidase conjugated streptavidin. Following an additional washing step, TMB substrate is used for color development at 450nm. A standard calibration curve is prepared along with the samples to be measured using dilutions of rat Factor XII. Color development is proportional to the concentration of Factor XII in the samples.
Product promise
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
Downloads
Product protocols
- Visit the general protocols
- Visit troubleshooting
Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.
For licensing inquiries, please contact partnerships@abcam.com