AB325033
Rat GCSF ELISA Kit (ab325033 ) is a sandwich ELISA to measure Rat GCSF in rat serum samples.
- Colorimetric ELISA kit - 450nm readout. Works on any standard plate reader
- Validated on rat serum
- Colorimetric ELISA kit - 450nm readout. Works on any standard plate reader
- Validated on rat serum
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- sELISA
Supplier Data
Sandwich ELISA - Rat GCSF ELISA Kit (AB325033)
Example standard curve for ab325033.
Reactivity data
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Product details
Rat GCSF ELISA Kit (ab324033) is a sandwich ELISA for the quantitative determination of GCSF in rat serum.
How the assay works
Microwells are pre-coated with anti-GCSF antibodies to capture recombinant rat GCSF protein from samples and standards pipetted into the wells. Following binding, a rabbit anti-rat IgG antibody is added and incubated. After washing to remove non-specific interactions, a ready-to-use TMB substrate is introduced, triggering a colorimetric reaction proportional to the concentration of recombinant rat GCSF in the sample. The reaction is halted by adding a stop solution, and absorbance is measured at 450 nm.
Rat GCSF ELISA Kit (ab325033 ) protocol summary
1. Add 100µL of standard or diluted samples to appropriate wells. Incubate for 60 min at 37°C
2. Wash
3. Add 100µL of Rabbit Anti Rat IgG:HRP Conjugate to each well. Incubate 60 min at 37°C
4. Wash
5. Add 100µL TMB substrate to each well. Incubate for 30 min at 37°C
6. Add 100µL of Stop solution to each well
7. Read at 450 nm with 30 min.
How the assay works
Microwells are pre-coated with anti-GCSF antibodies to capture recombinant rat GCSF protein from samples and standards pipetted into the wells. Following binding, a rabbit anti-rat IgG antibody is added and incubated. After washing to remove non-specific interactions, a ready-to-use TMB substrate is introduced, triggering a colorimetric reaction proportional to the concentration of recombinant rat GCSF in the sample. The reaction is halted by adding a stop solution, and absorbance is measured at 450 nm.
Rat GCSF ELISA Kit (ab325033 ) protocol summary
1. Add 100µL of standard or diluted samples to appropriate wells. Incubate for 60 min at 37°C
2. Wash
3. Add 100µL of Rabbit Anti Rat IgG:HRP Conjugate to each well. Incubate 60 min at 37°C
4. Wash
5. Add 100µL TMB substrate to each well. Incubate for 30 min at 37°C
6. Add 100µL of Stop solution to each well
7. Read at 450 nm with 30 min.
What's included?
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Properties and storage information
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
+4°C
Product protocols
- Visit the General protocols
- Visit the Troubleshooting
- Download websiteProtocolBooklet|en
Target data
Product promise
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