Rat GM-CSF ELISA Kit is a single-wash 90-min Simplestep used to quantify Rat GM-CSF with a sensitivity of 1.1 pg/ml. The assay uses a simple mix-wash-read protocol with just one incubation and one wash step.
- Colorimetric Sandwich ELISA - 450 nm readout : works on any standard plate reader
- Design your own immunoassay: we also offer the conjugation-ready antibody pair
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- Journal of the American Heart Association 9:e0149772019Renal Dopamine Oxidation and Inflammation in High Salt Fed Rats.Applications:Unspecified applicationReactive species:Unspecified reactive speciesAnees A Banday,Mustafa F LokhandwalaPubMed 31880979
Key facts
- Detection method
- Colorimetric
- Sample types
- Serum, Tissue Culture Media
- Assay type
- Sandwich (quantitative)
- Reactive species
- Rat
- Range
- 3.1 - 250 pg/mL
- Assay time
- 1h 30m
- Sensitivity
- = 1.1 pg/mL
Reactivity data
| Application | Reactivity | Dilution info | Notes |
|---|---|---|---|
Application sELISA | Reactivity Reacts | Dilution info - | Notes - |
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Target data
Function
Cytokine that stimulates the growth and differentiation of hematopoietic precursor cells from various lineages, including granulocytes, macrophages, eosinophils and erythrocytes.
Alternative names
Csfgm, Csf2, Granulocyte-macrophage colony-stimulating factor, GM-CSF, Colony-stimulating factor, CSF
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Rat GM-CSF ELISA Kit is a single-wash 90-min Simplestep used to quantify Rat GM-CSF with a sensitivity of 1.1 pg/ml. The assay uses a simple mix-wash-read protocol with just one incubation and one wash step.
- Colorimetric Sandwich ELISA - 450 nm readout : works on any standard plate reader
- Design your own immunoassay: we also offer the conjugation-ready antibody pair
Key facts
- Detection method
- Colorimetric
- Sample types
- Serum, Tissue Culture Media
- Assay type
- Sandwich (quantitative)
- Reactive species
- Rat
- Range
- 3.1 - 250 pg/mL
- Assay time
- 1h 30m
- Assay Platform
- Pre-coated microplate (12 x 8 well strips)
- Sensitivity
- = 1.1 pg/mL
Precision
Intra assay
| Sample | n | mean | SD | C.V. |
|---|---|---|---|---|
Sample Supernatant | n 5 | mean - | SD - | C.V. 2.2 |
Inter assay
| Sample | n | mean | SD | C.V. |
|---|---|---|---|---|
Sample Supernatant | n 3 | mean - | SD - | C.V. 3.7 |
Recovery
Sample specific recovery
| Sample type | Average % | Range |
|---|---|---|
Sample type Serum | Average % = 92 | Range 91 - 92 % |
Sample type Tissue Culture Media | Average % = 90 | Range 84 - 98 % |
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- +4°C
- Storage information
- +4°C
Notes
Rat GM-CSF ELISA Kit (ab236709) is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of GM-CSF protein in serum and tissue culture media. It uses our proprietary SimpleStep ELISA® technology. Quantitate Rat GM-CSF with 1.1 pg/ml sensitivity.
SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:
- Single-wash protocol reduces assay time to 90 minutes or less
- High sensitivity, specificity and reproducibility from superior antibodies
- Fully validated in biological samples
- 96-wells plate breakable into 12 x 8 wells strips
A 384-well SimpleStep ELISA® microplate (Pre-coated 384 well Microplate SimpleStep ELISA® ab203359) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
GM-CSF also known as Granulocyte-Macrophage Colony-Stimulating Factor is a glycoprotein with a mass of approximately 23 kDa. This protein plays a mechanical role in stimulating the differentiation and proliferation of hematopoietic progenitor cells into granulocytes and macrophages. Its expression occurs in various cell types including macrophages T cells endothelial cells and fibroblasts. GM-CSF interacts with its specific receptor known as CSF2 receptor to exert its effects.
- Biological function summary
GM-CSF influences the immune system by enhancing the functional capacity of mature neutrophils macrophages and eosinophils. GM-CSF protein acts alone and not as part of a larger protein complex. It is important in regulating immune responses and inflammation. Due to its role in promoting the survival and activation of these immune cells GM-CSF is important for mounting an effective immune defense in response to infections and other challenges.
- Pathways
GM-CSF signaling is integral to the JAK-STAT pathway. This pathway is essential for transmitting signals from surface receptors like GM-CSF receptors into the nucleus thereby inducing gene expression that leads to cell survival and proliferation. GM-CSF also intersects with the ERK1/ERK2 MAPK pathway which is involved in regulating cellular processes such as division and differentiation. The GM-CSF receptor shares similarities with other cytokine receptors involved in these pathways making it related to various cytokines through its downstream signaling effects.
- Associated diseases and disorders
GM-CSF has a well-established connection to diseases like rheumatoid arthritis and multiple sclerosis. In rheumatoid arthritis overproduction of GM-CSF is linked to chronic inflammation and joint damage. In multiple sclerosis GM-CSF might contribute to the pathological immune responses against the central nervous system. Antibodies targeting GM-CSF or its receptor represent a therapeutic strategy for these autoimmune diseases. By modulating GM-CSF activity therapeutic approaches aim to reduce inflammation and autoimmunity highlighting the protein's importance in disease progression.
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5 product images
Sandwich ELISA - Rat GM-CSF ELISA Kit (ab236709)
Rat GM-CSF competitor Std Curve Comparison.
Standard curve comparison between Rat GM-CSF SimpleStep ELISA® kit and traditional ELISA kit from leading competitor. SimpleStep ELISA kit shows increased sensitivity.
Sandwich ELISA - Rat GM-CSF ELISA Kit (ab236709)
Example of rat GM-CSF standard curve in Sample Diluent NS.
The GM-CSF standard curve was prepared as described in Section 10. Raw data values are shown in the table. Background-subtracted data values (mean +/- SD) are graphed.
Sandwich ELISA - Rat GM-CSF ELISA Kit (ab236709)
Example of rat GM-CSF standard curve in Sample Diluent 10BS.
The GM-CSF standard curve was prepared as described in Section 10. Raw data values are shown in the table. Background-subtracted data values (mean +/- SD) are graphed.
Sandwich ELISA - Rat GM-CSF ELISA Kit (ab236709)
Interpolated concentrations of native GM-CSF in rat lung supernatant samples.
The concentrations of GM-CSF were measured in duplicates, interpolated from the GM-CSF standard curves and corrected for sample dilution. Undiluted samples are as follows: rat lung supernatant 10% The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean GM-CSF concentration was determined to be 1534 pg/mL in rat lung supernatant.
Sandwich ELISA - Rat GM-CSF ELISA Kit (ab236709)
Interpolated concentrations of native GM-CSF in rat spleen supernatant samples.
Treated for three days with 50 ng/ml PMA and 500 ng/ml calcium ionomycin or untreated. The concentrations of GM-CSF were measured in duplicates, interpolated from the GM-CSF standard curves and corrected for sample dilution. Undiluted samples are as follows: treated spleen supernatant 1% and untreated spleen supernatant 100%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean GM-CSF concentration was determined to be 10.7 ng/mL in treated spleen supernatant and 150.6 pg/mL in untreated spleen supernatant samples.
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