JavaScript is disabled in your browser. Please enable JavaScript to view this website.
AB270219

Rat GPNMB ELISA Kit

Be the first to review this product! Submit a review

|

(0 Publication)

Rat GPNMB ELISA Kit is a single-wash 90-min Simplestep used to quantify Rat GPNMB with a sensitivity of 136 pg/ml. The assay uses a simple mix-wash-read protocol with just one incubation and one wash step.

- Colorimetric Sandwich ELISA - 450 nm readout : works on any standard plate reader
- Design your own immunoassay: we also offer the conjugation-ready antibody pair

View Alternative Names

Transmembrane glycoprotein NMB, Gpnmb

2 Images
Sandwich ELISA - Rat GPNMB ELISA Kit (AB270219)
  • sELISA

Supplier Data

Sandwich ELISA - Rat GPNMB ELISA Kit (AB270219)

Interpolated concentrations of native GPNMB in rat serum, plasma and cell culture supernatant samples.

The concentrations of GPNMB were measured in duplicates, interpolated from the GPNMB standard curves and corrected for sample dilution. Undiluted samples are as follows : serum 20%, plasma (citrate) 20%, plasma (EDTA) 40%, plasma (heparin) 20% and spleen supernatant 80%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean GPNMB concentration was determined to be 59 ng/mL in neat serum, 48 ng/mL in neat plasma (citrate), 41 ng/mL in neat plasma (EDTA), 69 ng/mL in neat plasma (heparin) and 24 ng/mL in neat spleen supernatant.

Sandwich ELISA - Rat GPNMB ELISA Kit (AB270219)
  • sELISA

Supplier Data

Sandwich ELISA - Rat GPNMB ELISA Kit (AB270219)

Example of rat GPNMB standard curve in Sample Diluent NS.

The GPNMB standard curve was prepared as described in Section 10. Raw data values are shown in the table. Background-subtracted data values (mean +/- SD) are graphed.

Key facts

Detection method

Colorimetric

Sample types

Cell culture media, Heparin Plasma, Citrate plasma, Cell culture supernatant, Serum, EDTA Plasma

Reacts with

Rat

Assay type

Sandwich (quantitative)

Sensitivity

= 136 pg/mL

Range

0.31 - 20 ng/mL

Assay time

1h 30m

Assay Platform

Pre-coated microplate (12 x 8 well strips)

style
left-column

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
style
left-column

Product details

Rat GPNMB ELISA Kit (ab270219) is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of GPNMB protein in cell culture media, cell culture supernatant, edta plasma, serum, cit plasma, and hep plasma. It uses our proprietary SimpleStep ELISA® technology. Quantitate Rat GPNMB with 136 pg/ml sensitivity.

SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:

- Single-wash protocol reduces assay time to 90 minutes or less
- High sensitivity, specificity and reproducibility from superior antibodies
- Fully validated in biological samples
- 96-wells plate breakable into 12 x 8 wells strips

A 384-well SimpleStep ELISA® microplate (ab203359) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.

GPNMB (Glycoprotein non-metastatic gene B), also known as Osteoactivin, is a type I transmembrane glycoprotein most often associated with endosomal/lysosomal compartment and over expressed in many types of cancer. The GPNMB is involved in many physiological processes, including transport of late melanosomes to keratinocytes, regulation of osteoblast and osteoclast, stimulation of dendritic cell maturation, and stimulation of adhesion of dendritic cells to endothelial cells.

style
left-column

Precision

[ { "reproducibilityType": "Intra", "sample": "Serum", "replicates": 7, "mean": null, "standardDeviation": null, "coefficientOfVariability": "4.3" } ]
style
left-column

Recovery

[ { "sample": "Cell culture supernatant", "range": "94 - 122 %", "average": "= 104" }, { "sample": "Serum", "range": "95 - 101 %", "average": "= 98" }, { "sample": "EDTA Plasma", "range": "97 - 112 %", "average": "= 102" }, { "sample": "Cell culture media", "range": "109 - 114 %", "average": "= 112" }, { "sample": "Heparin Plasma", "range": "94 - 121 %", "average": "= 105" }, { "sample": "Citrate plasma", "range": "112 - 133 %", "average": "= 120" } ]
style
left-column

What's included?

{ "values": { "1x96Tests": { "sellingSize": "1 x 96 Tests", "publicAssetCode":"ab270219-1x96Tests", "assetComponentDetails": [ { "size":"1 x 12 mL", "name":"Stop Solution", "number":"AB270219-CMP05", "productcode":"" }, { "size":"1 x 20 mL", "name":"10X Wash Buffer PT (ab206977)", "number":"AB270219-CMP10", "productcode":"" }, { "size":"1 x 50 mL", "name":"Sample Diluent NS (ab193972)", "number":"AB270219-CMP03", "productcode":"" }, { "size":"1 x 12 mL", "name":"TMB Development Solution", "number":"AB270219-CMP06", "productcode":"" }, { "size":"1 x 1 Unit", "name":"Plate Seals", "number":"AB270219-CMP09", "productcode":"" }, { "size":"1 x 600 µL", "name":"10X Rat GPNMB Capture Antibody", "number":"AB270219-CMP07", "productcode":"" }, { "size":"2 x 1 Vial", "name":"Rat GPNMB Lyophilized Recombinant Protein", "number":"AB270219-CMP02", "productcode":"" }, { "size":"1 x 6 mL", "name":"Antibody Diluent 4BR", "number":"AB270219-CMP01", "productcode":"" }, { "size":"1 x 600 µL", "name":"10X Rat GPNMB Detector Antibody", "number":"AB270219-CMP08", "productcode":"" }, { "size":"1 x 1 Unit", "name":"SimpleStep Pre-Coated 96-Well Microplate (ab206978)", "number":"AB270219-CMP04", "productcode":"" } ] } } }
style
left-column

Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
+4°C
style
left-column

Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

GPNMB also known as osteoactivin is a type I transmembrane glycoprotein with a molecular mass of approximately 115 kDa. This protein is highly expressed in various tissues including the bone skin and brain. It also shows notable presence in certain immune cells such as dendritic cells and macrophages. GPNMB localizes mainly on the cell membrane and within lysosomal compartments indicating its involvement in cellular and signaling processes.
Biological function summary

GPNMB plays versatile roles in promoting cell growth differentiation and repair. It is involved in processes like tissue regeneration possibly due to its role in cell adhesion and migration. The protein also partakes in osteoclast differentiation and bone resorption therefore linking to skeletal homeostasis. GPNMB forms part of a broader protein complex at the cellular junctions interacting with integrins to mediate its functions.

Pathways

GPNMB features prominently in osteoclastogenesis and melanoma metastasis pathways. It cooperates with RANKL an important protein in bone metabolism by influencing osteoclast differentiation and activation. In the context of melanoma GPNMB's action in pathways may enhance tumor proliferation and invasion possibly interacting with signaling molecules like AKT or ERK although the specifics remain actively researched.

GPNMB has significant links to both osteoarthritis and certain types of cancer particularly breast cancer and melanoma. In osteoarthritis GPNMB expression often increases reflecting involvement in cartilage degradation processes. Its overexpression correlates with tumor progression in melanoma potentially driven by its interaction with integrin β1 which assists in cellular adhesion and migration within the tumor microenvironment.
style
left-column

Product protocols

style
left-column

Target data

Could be a melanogenic enzyme.
See full target information Gpnmb
style
left-column
style
left-column
websiteProtocolBooklet
en
style
right-column

Complementary Products

Primary Antibodies

AB188222

Anti-GPNMB antibody [EPR18226-147]

RabMAb|Recombinant

Flow Cytometry (Intracellular) - Anti-GPNMB antibody [EPR18226-147] (AB188222)

  • 4
  • 1
Secondary Antibodies

AB150113

Goat Anti-Mouse IgG H&L (Alexa Fluor® 488)

Immunocytochemistry/ Immunofluorescence - Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) (AB150113)

  • 5
  • 15
Primary Antibodies

AB7817

Anti-alpha smooth muscle Actin antibody [1A4]

KO Validated

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-alpha smooth muscle Actin antibody [1A4] (AB7817)

  • 5
  • 105
Primary Antibodies

AB92547

Anti-Vimentin antibody [EPR3776] - Cytoskeleton Marker

BOND RX™ Validated|KO Validated|RabMAb|Recombinant|Lab Essentials|20ul selling size|Advanced Validation

Multiplex immunohistochemistry - Anti-Vimentin antibody [EPR3776] - Cytoskeleton Marker (AB92547)

  • 5
  • 92

Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com