JavaScript is disabled in your browser. Please enable JavaScript to view this website.
AB254508

Rat GSTA1 ELISA Kit

Be the first to review this product! Submit a review

|

(0 Publication)

Rat GSTA1 ELISA Kit is a single-wash 90-min Simplestep used to quantify Rat GSTA1 with a sensitivity of 0.35 ng/mL. The assay uses a simple mix-wash-read protocol with just one incubation and one wash step.

- Colorimetric Sandwich ELISA - 450 nm readout : works on any standard plate reader
- Design your own immunoassay: we also offer the conjugation-ready antibody pair

View Alternative Names

Glutathione S-transferase alpha-1, 13-hydroperoxyoctadecadienoate peroxidase, GST 1-1, GST 1a-1a, GST A1-1, GST B, Glutathione S-transferase Ya-1, Ligandin, GST Ya1, Gsta1

2 Images
Sandwich ELISA - Rat GSTA1 ELISA Kit (AB254508)
  • sELISA

Supplier Data

Sandwich ELISA - Rat GSTA1 ELISA Kit (AB254508)

Example of rat GSTA1 standard curve in Sample Diluent NS.

The GSTA1 standard curve was prepared as described in Section 10. Raw data values are shown in the table. Background-subtracted data values (mean +/- SD) are graphed.

Sandwich ELISA - Rat GSTA1 ELISA Kit (AB254508)
  • sELISA

Supplier Data

Sandwich ELISA - Rat GSTA1 ELISA Kit (AB254508)

Interpolated concentrations of native GSTA1 in rat cell culture supernatant samples.

The concentrations of GSTA1 were measured in duplicates, interpolated from the GSTA1 standard curves and corrected for sample dilution. Undiluted samples are as follows : liver supernatant 1 : 13,000. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean GSTA1 concentration was determined to be 271 μg/mL in liver supernatant.

Key facts

Detection method

Colorimetric

Sample types

Cell culture supernatant

Reacts with

Rat

Assay type

Sandwich (quantitative)

Sensitivity

= 0.35 ng/mL

Range

0.8 - 50 ng/mL

Assay time

1h 30m

Assay Platform

Pre-coated microplate (12 x 8 well strips)

style
left-column

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
style
left-column

Product details

Rat GSTA1 ELISA Kit (ab254508) is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of GSTA1 protein in cell culture supernatant. It uses our proprietary SimpleStep ELISA® technology. Quantitate Rat GSTA1 with 0.35 ng/ml sensitivity.

SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:

- Single-wash protocol reduces assay time to 90 minutes or less
- High sensitivity, specificity and reproducibility from superior antibodies
- Fully validated in biological samples
- 96-wells plate breakable into 12 x 8 wells strips

A 384-well SimpleStep ELISA® microplate (ab203359) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.

Glutathione S-transferase alpha-1 (GSTA1) is an enzyme in the GST family and is encoded by the GSTA1 gene. GSTA1s main role is the detoxification of electrophilic compounds, including carcinogens, drugs, environmental toxins, and by-products on environmental stress by conjugation with reduced glutathione. The alpha series of enzymes for GSH are activated in the liver. Rat GSTA1 shares a 96% sequence identify with GSTA2, and 94.6% with mouse GSTA1.

style
left-column

Precision

[ { "reproducibilityType": "Inter", "sample": "Supernatant", "replicates": 3, "mean": null, "standardDeviation": null, "coefficientOfVariability": "7.7" }, { "reproducibilityType": "Intra", "sample": "Supernatant", "replicates": 8, "mean": null, "standardDeviation": null, "coefficientOfVariability": "5.4" } ]
style
left-column

Recovery

[ { "sample": "Cell culture supernatant", "range": "95 - 107 %", "average": "= 102" } ]
style
left-column

What's included?

{ "values": { "1x96Tests": { "sellingSize": "1 x 96 Tests", "publicAssetCode":"ab254508-1x96Tests", "assetComponentDetails": [ { "size":"1 x 12 mL", "name":"Stop Solution", "number":"AB254508-CMP04", "productcode":"" }, { "size":"1 x 1 Unit", "name":"SimpleStep Pre-Coated 96-Well Microplate (ab206978)", "number":"AB254508-CMP03", "productcode":"" }, { "size":"2 x 1 Vial", "name":"10X Rat GSTA1 Detector Antibody", "number":"AB254508-CMP08", "productcode":"" }, { "size":"1 x 6 mL", "name":"Antibody Diluent 5BR", "number":"AB254508-CMP01", "productcode":"" }, { "size":"1 x 1 Unit", "name":"Plate Seals", "number":"AB254508-CMP09", "productcode":"" }, { "size":"1 x 20 mL", "name":"10X Wash Buffer PT (ab206977)", "number":"AB254508-CMP10", "productcode":"" }, { "size":"1 x 12 mL", "name":"TMB Development Solution", "number":"AB254508-CMP05", "productcode":"" }, { "size":"1 x 50 mL", "name":"Sample Diluent NS (ab193972)", "number":"AB254508-CMP02", "productcode":"" }, { "size":"2 x 1 Vial", "name":"Rat GSTA1 Lyophilized Recombinant Protein", "number":"AB254508-CMP06", "productcode":"" }, { "size":"1 x 600 µL", "name":"10X Rat GSTA1 Capture Antibody", "number":"AB254508-CMP07", "productcode":"" } ] } } }
style
left-column

Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
+4°C
style
left-column

Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

The GSTA1 protein also known as Glutathione S-transferase alpha 1 is an important enzyme in detoxification processes in the body. It has a molecular mass of approximately 25 kDa and expresses highly in the liver but is also seen in other tissues like the kidney and lung. It belongs to the glutathione S-transferase family which plays function in the conjugation of reduced glutathione to a variety of endogenous and exogenous electrophilic compounds.
Biological function summary

The GSTA1 protein contributes significantly to the neutralization of oxidative stress by detoxifying harmful compounds. This enzyme shows a high affinity for certain substrates and serves as a detoxifying agent particularly in the liver safeguarding cells from oxidative damage. GSTA1 exists mostly as a homodimer but can also form heterodimers with other related enzymes enhancing its substrate range and activity.

Pathways

GSTA1 plays a role in metabolic and detoxification pathways essential for cellular protection. It is an important player in the glutathione metabolism pathway where it collaborates with other enzymes like GSTM1 to facilitate detoxification processes. Additionally GSTA1 participates in the pathways leading to the synthesis of mercapturic acids which are important for the excretion of toxic metabolites.

GSTA1 is linked to conditions such as cancer and cardiovascular diseases. Altered expression or polymorphisms in the GSTA1 gene can affect the enzyme's ability to detoxify carcinogens making it significant in cancer risk and progression. The GSTA1 protein also interacts with other enzymes like GSTA4 in the context of oxidative stress which relates to cardiovascular disorder pathogenesis.
style
left-column

Product protocols

style
left-column

Target data

Glutathione S-transferase that catalyzes the nucleophilic attack of the sulfur atom of glutathione on the electrophilic groups of a wide range of exogenous and endogenous compounds (Probable). Involved in the formation of glutathione conjugates of both prostaglandin A2 (PGA2) and prostaglandin J2 (PGJ2). It also catalyzes the isomerization of D5-androstene-3,17-dione (AD) into D4-androstene-3,17-dione and may therefore play an important role in hormone biosynthesis. Through its glutathione-dependent peroxidase activity toward the fatty acid hydroperoxide (13S)-hydroperoxy-(9Z,11E)-octadecadienoate/13-HPODE it is also involved in the metabolism of oxidized linoleic acid (By similarity).
See full target information Glutathione S-transferase alpha-1
style
left-column
style
left-column
websiteProtocolBooklet
en
style
right-column

Complementary Products

Assay Kits

AB255716

Rat ST2 ELISA Kit (IL1RL1)

Recombinant|SimpleStep

Sandwich ELISA - Rat ST2 ELISA Kit (IL1RL1) (AB255716)

  • 0
  • 0
Primary Antibodies

AB180650

Anti-GSTA1 antibody

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GSTA1 antibody (AB180650)

  • 4
  • 1
Assay Kits

AB65326

GST Assay Kit (Colorimetric)

Functional Studies - GST Assay Kit (Colorimetric) (AB65326)

  • 0
  • 0
Assay Kits

AB239422

Rat Lipocalin-2 ELISA Kit

Recombinant|SimpleStep

Sandwich ELISA - Rat Lipocalin-2 ELISA Kit (AB239422)

  • 0
  • 0

Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com