AB279414

Rat Heme Oxygenase 1 ELISA Kit

Recombinant
SimpleStep
What is this?

Be the first to review this product! Submit a review

(2 Publications)

Rat Heme Oxygenase 1 ELISA Kit is a single-wash 90-min Simplestep used to quantify Rat Heme Oxygenase 1 with a sensitivity of 3.03 pg/ml. The assay uses a simple mix-wash-read protocol with just one incubation and one wash step.

- Colorimetric Sandwich ELISA - 450 nm readout : works on any standard plate reader
- Design your own immunoassay: we also offer the conjugation-ready antibody pair
- Cited in over 5 citations

View Alternative Names

Heme oxygenase 1, HO-1, HSP32, Hmox1

3 Images

Sandwich ELISA - Rat Heme Oxygenase 1 ELISA Kit (AB279414)

sELISA

Supplier Data

Sandwich ELISA - Rat Heme Oxygenase 1 ELISA Kit (AB279414)

Example of rat Heme Oxygenase 1 standard curve in Sample Diluent NS + 1X Enhancer.

The Heme Oxygenase 1 standard curve was prepared as described in Section 10. Raw data values are shown in the table. Background-subtracted data values (mean +/- SD) are graphed.

sELISA

Supplier Data

Sandwich ELISA - Rat Heme Oxygenase 1 ELISA Kit (AB279414)

Interpolated concentrations of native Heme Oxygenase 1 in rat serum and plasma samples.

The concentrations of Heme Oxygenase 1 were measured in duplicates, interpolated from the Heme Oxygenase 1 standard curves and corrected for sample dilution. Undiluted samples are as follows : serum 25%, plasma (citrate) 25%, plasma (EDTA) 50%, and plasma (heparin) 25%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean Heme Oxygenase 1 concentration was determined to be 1,611 pg/mL in serum, 954.9 pg/mL in plasma (citrate), 565.9 pg/mL in plasma (EDTA), and 1,260 pg/mL in plasma (heparin).

sELISA

Supplier Data

Sandwich ELISA - Rat Heme Oxygenase 1 ELISA Kit (AB279414)

Interpolated concentrations of native Heme Oxygenase 1 in rat PC-12 cell extract sample based on a 4,000 ng/mL extract load.

The concentrations of Heme Oxygenase 1 were measured in duplicate and interpolated from the Heme Oxygenase 1 standard curve and corrected for sample dilution. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean Heme Oxygenase 1 concentration was determined to be 741.7 pg/mL in sample.

Key facts

Detection method

Colorimetric

Sample types

Cell culture media, Heparin Plasma, Serum, Cell Lysate, EDTA Plasma

Reacts with

Rat

Assay type

Sandwich (quantitative)

Sensitivity

= 3.03 pg/mL

Range

15.63 - 1000 pg/mL

Assay time

1h 30m

Assay Platform

Pre-coated microplate (12 x 8 well strips)

style

left-column

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }

style

left-column

Product details

Rat Heme Oxygenase 1 ELISA kit (ab277414) is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of Rat Heme Oxygenase 1 protein in rat serum, plasma, cell culture supernatant and cell and tissue extract samples. It uses our proprietary SimpleStep ELISA® technology. Quantitate Rat Heme Oxygenase 1 with 3.03 pg/mL sensitivity.

SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:

-Single-wash protocol reduces assay time to 90 minutes or less
-High sensitivity, specificity and reproducibility from superior antibodies
-Fully validated in biological samples
-96-wells plate breakable into 12 x 8 wells strips

A 384-well SimpleStep ELISA® microplate (ab203359) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.

Heme oxygenase 1 is an enzyme that functions in heme catabolism. The activity of heme oxygenase 1 is to cleave the heme ring to form biliverdin. There are two isozymes of heme oxygenase that have 41% amino acid sequence identity: inducible heme oxygenase 1 and constitutively expressed heme oxygenase 2.

REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.

style

left-column

Precision

[ { "reproducibilityType": "Inter", "sample": "Serum", "replicates": 3, "mean": null, "standardDeviation": null, "coefficientOfVariability": "2.96" }, { "reproducibilityType": "Intra", "sample": "Serum", "replicates": 8, "mean": null, "standardDeviation": null, "coefficientOfVariability": "2.9" } ]

style

left-column

Recovery

[ { "sample": "Serum", "range": "83 - 92 %", "average": "= 86" }, { "sample": "EDTA Plasma", "range": "83 - 95 %", "average": "= 90" }, { "sample": "Cell culture media", "range": "108 - 118 %", "average": "= 113" }, { "sample": "Heparin Plasma", "range": "88 - 93 %", "average": "= 90" }, { "sample": "Citrate plasma", "range": "88 - 95 %", "average": "= 91" } ]

style

left-column

What's included?

{ "values": { "1x96Tests": { "sellingSize": "1 x 96 Tests", "publicAssetCode":"ab279414-1x96Tests", "assetComponentDetails": [ { "size":"1 x 12 mL", "name":"Stop Solution", "number":"AB279414-CMP05", "productcode":"" }, { "size":"1 x 20 mL", "name":"10X Wash Buffer PT (ab206977)", "number":"AB279414-CMP12", "productcode":"" }, { "size":"1 x 1 mL", "name":"50X Cell Extraction Enhancer Solution (ab193971)", "number":"AB279414-CMP13", "productcode":"" }, { "size":"1 x 50 mL", "name":"Sample Diluent NS (ab193972)", "number":"AB279414-CMP03", "productcode":"" }, { "size":"1 x 12 mL", "name":"TMB Development Solution", "number":"AB279414-CMP06", "productcode":"" }, { "size":"1 x 1 Unit", "name":"Plate Seals", "number":"AB279414-CMP11", "productcode":"" }, { "size":"1 x 600 µL", "name":"10X Rat Heme Oxygenase 1 Capture Antibody", "number":"AB279414-CMP10", "productcode":"" }, { "size":"2 x 1 Vial", "name":"Rat Heme Oxygenase 1 Lyophilized Recombinant Protein", "number":"AB279414-CMP09", "productcode":"" }, { "size":"1 x 6 mL", "name":"Antibody Diluent 4BR", "number":"AB279414-CMP01", "productcode":"" }, { "size":"1 x 10 mL", "name":"5X Cell Extraction Buffer PTR (ab193970)", "number":"AB279414-CMP08", "productcode":"" }, { "size":"1 x 600 µL", "name":"10X Rat Heme Oxygenase 1 Detector Antibody", "number":"AB279414-CMP02", "productcode":"" }, { "size":"1 x 1 Unit", "name":"SimpleStep Pre-Coated 96-Well Microplate (ab206978)", "number":"AB279414-CMP04", "productcode":"" } ] } } }

style

left-column

Properties and storage information

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

+4°C

Storage information

+4°C

style

left-column

Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Heme Oxygenase 1 also known as HO-1 or HMOX1 is an enzyme that plays an important mechanistic role in heme catabolism. It catalyzes the degradation of heme into biliverdin carbon monoxide and free iron. This process involves the cleavage of the heme ring. HO-1 has a molecular weight of approximately 32 kDa. It is widely expressed in numerous tissues but is especially abundant in the liver and spleen. Its expression is induced by heme and other stress stimuli such as heavy metals cytokines and reactive oxygen species.

Biological function summary

Heme Oxygenase 1 serves important protective functions in the body. It is not part of a larger complex but its products such as carbon monoxide and biliverdin have their own biological activities. Carbon monoxide produced by HO-1 has antiflammatory properties and can modulate apoptotic pathways. Biliverdin is reduced to bilirubin which acts as an antioxidant. The enzyme therefore directly influences cellular stress responses and maintains cellular homeostasis through these processes.

Pathways

Heme Oxygenase 1 is integrally involved in oxidative stress response and heme metabolism. It participates in the cellular response to oxidative damage by reducing oxidative stress and promoting cytoprotection. Through its heme degradation activity it is connected with the synthesis of biologically active molecules like bilirubin and carbon monoxide. Heme Oxygenase 1 activity is related to other proteins in oxidative stress pathways such as Nuclear Factor Erythroid 2-Related Factor 2 (Nrf2) which regulates its expression and globins which are sources of heme for HO-1 activity.

Heme Oxygenase 1 has been linked to conditions like cardiovascular diseases and neurodegenerative disorders. Its expression can attenuate the severity of atherosclerosis where oxidative stress is an important factor. In neurodegenerative diseases HO-1’s antioxidant properties may provide neuroprotection by mitigating oxidative damage. The protein's interactions with inflammatory cytokines such as Interleukin-6 and tumor necrosis factor-alpha influence its activity in these disease contexts.

style

left-column

Product protocols

style

left-column

Target data

Heme oxygenase 1. Catalyzes the oxidative cleavage of heme at the alpha-methene bridge carbon, released as carbon monoxide (CO), to generate biliverdin IXalpha, while releasing the central heme iron chelate as ferrous iron (PubMed : 1575508, PubMed : 1935972, PubMed : 3865203). Affords protection against programmed cell death and this cytoprotective effect relies on its ability to catabolize free heme and prevent it from sensitizing cells to undergo apoptosis (By similarity).. Heme oxygenase 1 soluble form. Catalyzes the oxidative cleavage of heme at the alpha-methene bridge carbon, released as carbon monoxide (CO), to generate biliverdin IXalpha, while releasing the central heme iron chelate as ferrous iron.

See full target information Hmox1

style

left-column

Publications (2)

Recent publications for all applications. Explore the full list and refine your search

Saudi pharmaceutical journal : SPJ : the official publication of the Saudi Pharmaceutical Society 31:101817 PubMed37915829

2023

Protective effect of eriodictyol against hyperglycemia-induced diabetic nephropathy in rats entails antioxidant and anti-inflammatory effects mediated by activating Nrf2.

Applications

Unspecified application

Species

Unspecified reactive species

Jozaa Z AlTamimi,Nora A AlFaris,Ghedeir M Alshammari,Reham I Alagal,Dalal H Aljabryn,Mohammed Abdo Yahya

Pharmaceuticals (Basel, Switzerland) 16: PubMed37111238

2023

Dihydromyricetin Modulates Nrf2 and NF-κB Crosstalk to Alleviate Methotrexate-Induced Lung Toxicity.

Applications

Unspecified application

Species

Unspecified reactive species

Asmaa I Matouk,Eman M Awad,Nashwa F G El-Tahawy,Azza A K El-Sheikh,Aliaa Anter

View all publications

style

left-column

style

left-column

websiteProtocolBooklet

style

right-column

Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Rat Heme Oxygenase 1 ELISA Kit

Sandwich ELISA - Rat Heme Oxygenase 1 ELISA Kit (AB279414)

Sandwich ELISA - Rat Heme Oxygenase 1 ELISA Kit (AB279414)

Sandwich ELISA - Rat Heme Oxygenase 1 ELISA Kit (AB279414)

Key facts

Detection method

Sample types

Reacts with

Assay type

Sensitivity

Range

Assay time

Assay Platform

Reactivity data

Product details

Precision

Recovery

What's included?

Properties and storage information

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (2)

Complementary Products

Assay Kits

Secondary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Product promise