Rat Hemoglobin ELISA Kit is a sandwich ELISA designed to quantify Rat Hemoglobin with a sensitivity of 1.4284 ng/mL.
- Colorimetric sandwich ELISA - 450 nm readout - works on any plate reader
- Wide dynamic range - quantifies 0.006 - 16.92 µg/mL
View Alternative Names
Hba-a1, Hba1, Hemoglobin subunit alpha-1/2, Alpha-1/2-globin, Hemoglobin alpha-1/2 chain
- sELISA
Supplier Data
Sandwich ELISA - Rat Hemoglobin ELISA Kit (AB157733)
Representative standard curve using ab157733 Hemoglobin Rat ELISA Kit.
Reactivity data
Product details
How the assay works
In this assay the Hemoglobin present in samples reacts with the anti-Hemoglobin antibodies which have been adsorbed to the surface of polystyrene microtitre wells. After the removal of unbound proteins by washing, anti-Hemoglobin antibodies conjugated with horseradish peroxidase (HRP) are added. These enzyme-labeled antibodies form complexes with the previously bound Hemoglobin. Following another washing step, the enzyme bound to the immunosorbent is assayed by the addition of a chromogenic substrate, 3,3',5,5'-tetramethylbenzidine (TMB). The quantity of bound enzyme varies directly with the concentration of Hemoglobin in the sample tested; thus, the absorbance, at 450 nm, is a measure of the concentration of Hemoglobin in the test sample. The quantity ofHemoglobinin the test sample can be interpolated from the standard curve constructed from the standards, and corrected for sample dilution.
Assay Specificity
Our ELISA kits are rigorously validated to ensure the highest level of consistency and reproducibility. Please check the protocol booklet for more details
Rat Hemoglobin ELISA Kit ab157733 protocol summary
1. Add standard or sample to each well used. Incubate at room temperature.
2. Aspirate and wash each well.
3. Add prepared HRP labeled secondary detector antibody. Incubate at room temperature
4. Aspirate and wash each well.
5. Add Chromogen Substrate Solution to each well.
6. Immediately begin recording the color development
Precision
Recovery
What's included?
Properties and storage information
Shipped at conditions
Appropriate short-term storage conditions
Appropriate long-term storage conditions
Storage information
Supplementary information
This supplementary information is collated from multiple sources and compiled automatically.
Biological function summary
Hemoglobin carries oxygen in blood and acts as a buffer in maintaining blood pH. Within the erythrocytes hemoglobin comprises one of the principal components of these cells each molecule binding up to four oxygen molecules. Hemoglobin exists as a complex with heme groups that directly interact with oxygen allowing hemoglobin to perform its respiratory function efficiently.
Pathways
Hemoglobin is important in the circulatory and respiratory pathways. It works in conjunction with proteins like myoglobin which assists with oxygen storage in muscles and cytochrome c which is important in the electron transport chain. Hemoglobin's function aligns with these pathways to maintain proper oxygen and energy levels throughout the body.
Product protocols
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Target data
Publications (1)
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Biopharmaceutics & drug disposition 44:71-83 PubMed35508078
2022
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Species
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