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AB157734

Rat Hemopexin ELISA Kit (HPX)

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(1 Publication)

Rat Hemopexin ELISA Kit (HPX) is a Sandwich (quantitative) ELISA for the measurement of Rat Hemopexin (HPX) in Rat in Biofluids samples.

View Alternative Names

Hemopexin, Hpx

1 Images
Sandwich ELISA - Rat Hemopexin ELISA Kit (HPX) (AB157734)
  • sELISA

Supplier Data

Sandwich ELISA - Rat Hemopexin ELISA Kit (HPX) (AB157734)

Representative standard curve using ab157734 Hemopexin Rat ELISA Kit.

Key facts

Detection method

Colorimetric

Sample types

Plasma, Serum, Other biological fluids

Reacts with

Rat

Assay type

Sandwich (quantitative)

Sensitivity

= 1.1 ng/mL

Range

6.25 - 400 ng/mL

Assay Platform

Microplate

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Product details

Abcam's Hemopexin (HPX) Rat ELISA Kit is a highly sensitive two-site enzyme linked immunoassay (ELISA) for measuring Hemopexin (HX) in biological samples of rats.

In this assay the Hemopexin present in samples reacts with the anti-Hemopexin antibodies which have been adsorbed to the surface of polystyrene microtitre wells. After the removal of unbound proteins by washing, anti-HX antibodies conjugated with horseradish peroxidase (HRP) are added. These enzyme-labeled antibodies form complexes with the previously bound HX. Following another washing step, the enzyme bound to the immunosorbent is assayed by the addition of a chromo­genic substrate, 3,3',5,5'-tetramethylbenzidine (TMB). The quantity of bound enzyme varies directly with the concentration of HX in the sample tested; thus, the absorbance, at 450 nm, is a measure of the concentration of HX in the test sample. The quantity of HX in the test sample can be interpolated from the standard curve constructed from the standards, and corrected for sample dilution.

ab157734 has been redeveloped with new goat capture and detector antibodies, Kits purchased prior to 2020 used chicken antibodies.

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Precision

[ { "reproducibilityType": "Inter", "sample": "Overall", "replicates": 0, "mean": null, "standardDeviation": null, "coefficientOfVariability": "< 10" }, { "reproducibilityType": "Intra", "sample": "Overall", "replicates": 0, "mean": null, "standardDeviation": null, "coefficientOfVariability": "< 10" } ]
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Recovery

[ { "sample": "Serum", "range": null, "average": "> 85" } ]
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What's included?

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Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
+4°C
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Hemopexin also known as HPX is a glycoprotein that transports heme in the blood. It has a molecular weight of approximately 57 kDa and is primarily expressed in the liver. Hemopexin binds to free heme with high affinity which prevents oxidative damage and conserves iron. It transports the heme to hepatocytes for breakdown and recycling protecting cells from iron-mediated toxicity and oxidative stress.
Biological function summary

The hemopexin function relates to protecting tissues from the harmful effects of free heme. Hemopexin does not work as part of a complex in this process but mainly acts independently. Its high binding affinity plays an important role in maintaining heme homeostasis in the plasma and tissues. By controlling heme levels hemopexin supports overall cellular health and function.

Pathways

The hemopexin molecular weight allows it to fit effectively within the heme metabolism and iron recycling pathways. This protein plays a significant role by delivering heme to the liver and facilitating its degradation by heme oxygenase. Hemopexin interacts closely with heme oxygenase and albumin two proteins important for the management of heme and iron levels in the body.

Several hemopexin functions contribute to the understanding of conditions such as hemolytic anemia and acute liver injury. In hemolytic anemia hemopexin helps mitigate the effects of increased heme levels due to excessive red blood cell breakdown. In the context of liver injury the protein assists in the detoxification process and supports hepatic function. Hemopexin-related proteins such as heme oxygenase link these diseases to hemopexin by their roles in managing oxidative stress and heme catabolism.
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Product protocols

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Target data

Binds heme and transports it to the liver for breakdown and iron recovery, after which the free hemopexin returns to the circulation.
See full target information Hemopexin
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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Redox biology 14:423-438 PubMed29078168

2017

STAT3-RXR-Nrf2 activates systemic redox and energy homeostasis upon steep decline in pO gradient.

Applications

ELISA

Species

Rat

Subhojit Paul,Anamika Gangwar,Kalpana Bhargava,Yasmin Ahmad
View all publications
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websiteProtocolBooklet
en
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Product promise

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