Rat Hemopexin ELISA Kit (HPX) is a Sandwich (quantitative) ELISA kit for the measurement of Rat Hemopexin (HPX) in Rat in Plasma, Serum, Other biological fluids samples.
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- Redox biology 14:423-4382017STAT3-RXR-Nrf2 activates systemic redox and energy homeostasis upon steep decline in pO gradient.Applications:ELISAReactive species:RatSubhojit Paul,Anamika Gangwar,Kalpana Bhargava,Yasmin AhmadPubMed 29078168
Key facts
- Detection method
- Colorimetric
- Sample types
- Plasma, Serum, Other biological fluids
- Assay type
- Sandwich (quantitative)
- Reactive species
- Rat
- Range
- 6.25 - 400 ng/mL
- Sensitivity
- = 1.1 ng/mL
Reactivity data
| Application | Reactivity | Dilution info | Notes |
|---|---|---|---|
Application sELISA | Reactivity Reacts | Dilution info - | Notes - |
Target data
Function
Binds heme and transports it to the liver for breakdown and iron recovery, after which the free hemopexin returns to the circulation.
Alternative names
Hemopexin, Hpx
What's included?
Recommended products
Rat Hemopexin ELISA Kit (HPX) is a Sandwich (quantitative) ELISA kit for the measurement of Rat Hemopexin (HPX) in Rat in Plasma, Serum, Other biological fluids samples.
Key facts
- Detection method
- Colorimetric
- Sample types
- Plasma, Serum, Other biological fluids
- Assay type
- Sandwich (quantitative)
- Reactive species
- Rat
- Range
- 6.25 - 400 ng/mL
- Assay Platform
- Microplate
- Sensitivity
- = 1.1 ng/mL
Precision
Intra assay
| Sample | n | mean | SD | C.V. |
|---|---|---|---|---|
Sample Overall | n 0 | mean - | SD - | C.V. < 10 |
Inter assay
| Sample | n | mean | SD | C.V. |
|---|---|---|---|---|
Sample Overall | n 0 | mean - | SD - | C.V. < 10 |
Recovery
Sample specific recovery
| Sample type | Average % | Range |
|---|---|---|
Sample type Serum | Average % > 85 | Range |
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- +4°C
- Storage information
- +4°C
Notes
Abcam's Hemopexin (HPX) Rat ELISA Kit is a highly sensitive two-site enzyme linked immunoassay (ELISA) for measuring Hemopexin (HX) in biological samples of rats.
In this assay the Hemopexin present in samples reacts with the anti-Hemopexin antibodies which have been adsorbed to the surface of polystyrene microtitre wells. After the removal of unbound proteins by washing, anti-HX antibodies conjugated with horseradish peroxidase (HRP) are added. These enzyme-labeled antibodies form complexes with the previously bound HX. Following another washing step, the enzyme bound to the immunosorbent is assayed by the addition of a chromogenic substrate, 3,3',5,5'-tetramethylbenzidine (TMB). The quantity of bound enzyme varies directly with the concentration of HX in the sample tested; thus, the absorbance, at 450 nm, is a measure of the concentration of HX in the test sample. The quantity of HX in the test sample can be interpolated from the standard curve constructed from the standards, and corrected for sample dilution.
ab157734 has been redeveloped with new goat capture and detector antibodies, Kits purchased prior to 2020 used chicken antibodies.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
Hemopexin also known as HPX is a glycoprotein that transports heme in the blood. It has a molecular weight of approximately 57 kDa and is primarily expressed in the liver. Hemopexin binds to free heme with high affinity which prevents oxidative damage and conserves iron. It transports the heme to hepatocytes for breakdown and recycling protecting cells from iron-mediated toxicity and oxidative stress.
- Biological function summary
The hemopexin function relates to protecting tissues from the harmful effects of free heme. Hemopexin does not work as part of a complex in this process but mainly acts independently. Its high binding affinity plays an important role in maintaining heme homeostasis in the plasma and tissues. By controlling heme levels hemopexin supports overall cellular health and function.
- Pathways
The hemopexin molecular weight allows it to fit effectively within the heme metabolism and iron recycling pathways. This protein plays a significant role by delivering heme to the liver and facilitating its degradation by heme oxygenase. Hemopexin interacts closely with heme oxygenase and albumin two proteins important for the management of heme and iron levels in the body.
- Associated diseases and disorders
Several hemopexin functions contribute to the understanding of conditions such as hemolytic anemia and acute liver injury. In hemolytic anemia hemopexin helps mitigate the effects of increased heme levels due to excessive red blood cell breakdown. In the context of liver injury the protein assists in the detoxification process and supports hepatic function. Hemopexin-related proteins such as heme oxygenase link these diseases to hemopexin by their roles in managing oxidative stress and heme catabolism.
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1 product image
Sandwich ELISA - Rat Hemopexin ELISA Kit (HPX) (ab157734)
Representative standard curve using ab157734 Hemopexin Rat ELISA Kit.
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Product protocols
- Visit the general protocols
- Visit troubleshooting
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