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AB285233

Rat Hepcidin ELISA Kit

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Rat Hepcidin ELISA Kit is a Sandwich ELISA for the measurement of Rat Hepcidin in Rat in Biofluids, Cell/Tissue Extracts samples.

View Alternative Names

Hepc, Hamp, Hepcidin

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Sandwich ELISA - Rat Hepcidin ELISA Kit (AB285233)
  • sELISA

Supplier Data

Sandwich ELISA - Rat Hepcidin ELISA Kit (AB285233)

Hepcidin (Rat) Elisa Kit Standard Curve.

Typical Standard Curve : These standard curves are for demonstration only. A standard curve must be run with each assay.

Key facts

Detection method

Colorimetric

Sample types

Plasma, Tissue Homogenate, Serum, Other biological fluids

Reacts with

Rat

Assay type

Sandwich

Sensitivity

< 9.375 pg/mL

Range

15.625 - 1000 pg/mL

Assay Platform

Microplate (12 x 8 well strips)

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Product details

Hepcidin is a liver-produced hormone that constitutes the main circulating regulator of iron absorption and distribution across tissues. Acts by promoting endocytosis and degradation of ferroportin, leading to the retention of iron in iron-exporting cells and decreased flow of iron into plasma. Controls the major flows of iron into plasma: absorption of dietary iron in the intestine, recycling of iron by macrophages, which phagocytose old erythrocytes and other cells, and mobilization of stored iron from hepatocytes.

Rat Hepcidin ELISA Kit (ab285233, E4694) is a sandwich ELISA assay for the quantitative measurement of Hepcidin in rat serum, plasma and cell culture supernatants. The density of color is proportional to the amount of Hepcidin captured from the samples.

This product is manufactured by BioVision, an Abcam company and was previously called E4694 Hepcidin (Rat) ELISA Kit. E4694-100 is the same size as the 96 test size of ab285233.

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Precision

[ { "reproducibilityType": "Intra", "sample": "Overall", "replicates": 0, "mean": null, "standardDeviation": null, "coefficientOfVariability": "< 8" }, { "reproducibilityType": "Inter", "sample": "Overall", "replicates": 0, "mean": null, "standardDeviation": null, "coefficientOfVariability": "< 10" } ]
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What's included?

{ "values": { "96Test": { "sellingSize": "96 Test", "publicAssetCode":"ab285233-96Test", "assetComponentDetails": [ { "size":"5 x 1 Unit", "name":"Plate Sealer", "number":"AB285233-CMP05", "productcode":"" }, { "size":"1 x 1 Unit", "name":"Micro ELISA Plate", "number":"AB285233-CMP04", "productcode":"" }, { "size":"1 x 10 mL", "name":"Stop Solution", "number":"AB285233-CMP08", "productcode":"" }, { "size":"2 x 1 Vial", "name":"Lyophilized Standard", "number":"AB285233-CMP06", "productcode":"" }, { "size":"1 x 10 mL", "name":"SABC dilution buffer", "number":"AB285233-CMP07", "productcode":"" }, { "size":"1 x 10 mL", "name":"TMB Substrate", "number":"AB285233-CMP10", "productcode":"" }, { "size":"1 x 120 µL", "name":"HRP-Streptavidin Conjugate (SABC)", "number":"AB285233-CMP09", "productcode":"" }, { "size":"1 x 120 µL", "name":"Biotin-Detection antibody (Concentrated)", "number":"AB285233-CMP03", "productcode":"" }, { "size":"1 x 20 mL", "name":"Sample and Standard Dilution Buffer", "number":"AB285233-CMP02", "productcode":"" }, { "size":"1 x 10 mL", "name":"Antibody dilution buffer", "number":"AB285233-CMP01", "productcode":"" }, { "size":"1 x 30 mL", "name":"25X Wash buffer", "number":"AB285233-CMP11", "productcode":"" } ] } } }
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Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
+4°C
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Hepcidin also known as Liver-Expressed Antimicrobial Peptide (LEAP) or HEPC is an important regulator of iron homeostasis functioning as a hormone. It consists of a small peptide typically around 25 amino acids in length with a molecular mass of approximately 2.8 kDa. Hepcidin is produced predominantly in the liver and is secreted into the bloodstream where it directly binds to the iron export protein ferroportin. This binding induces ferroportin's internalization and degradation effectively restricting iron release into the circulation and controlling systemic iron levels.
Biological function summary

This hormone plays a critical role in maintaining iron balance within the body by regulating both the absorption of dietary iron in the intestine and the release of stored iron from macrophages and hepatocytes. Hepcidin operates independently and is not part of any larger complex. Its levels fluctuate in response to various stimuli including iron levels inflammation and erythropoietic demand impacting iron transport and storage.

Pathways

Hepcidin is integral to the iron metabolism pathway. It interacts intricately with proteins such as transferrin and ferritin which are involved in iron transport and storage. Hepcidin's function is modulated by the BMP/SMAD signaling pathway as well where its expression is promoted by these signaling proteins. This regulation ensures that iron levels are properly maintained for critical processes such as hemoglobin synthesis and cellular respiration.

Hepcidin is connected to conditions such as anemia of chronic disease and hereditary hemochromatosis. In anemia of chronic disease increased hepcidin levels lead to iron sequestration and restricted availability which contributes to the anemic state. In hereditary hemochromatosis mutations can result in decreased hepcidin activity causing excessive iron accumulation in the body. Through these mechanisms hepcidin's interactions with proteins such as ferroportin and transferrin receptor 2 influence the development and progression of these iron-related disorders.
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Product protocols

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Target data

Liver-produced hormone that constitutes the main circulating regulator of iron absorption and distribution across tissues. Acts by promoting endocytosis and degradation of ferroportin/SLC40A1, leading to the retention of iron in iron-exporting cells and decreased flow of iron into plasma. Controls the major flows of iron into plasma : absorption of dietary iron in the intestine, recycling of iron by macrophages, which phagocytose old erythrocytes and other cells, and mobilization of stored iron from hepatocytes.. Has strong antimicrobial activity against E.coli ML35P N.cinerea and weaker against S.epidermidis, S.aureus and group b streptococcus bacteria. Active against the fungus C.albicans. No activity against P.aeruginosa.
See full target information Hamp
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websiteProtocolBooklet
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Product promise

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