AB322375

Rat Hepcidin ELISA Kit

Recombinant
SimpleStep
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Rat Hepcidin ELISA Kit is a Sandwich (quantitative) ELISA for the measurement of Rat Hepcidin in Rat in Biofluids, Cell Culture Media samples.

View Alternative Names

Hepc, Hamp, Hepcidin

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Sandwich ELISA - Rat Hepcidin ELISA Kit (AB322375)

sELISA

Supplier Data

Sandwich ELISA - Rat Hepcidin ELISA Kit (AB322375)

Interpolated concentration of native Hepcidin was measured in duplicate at different sample concentrations in 96-well vs. 384-well plates. Undiluted samples are 5% rat serum. The interpolated dilution factor corrected values (to neat sample) are plotted (mean +/- SD, n=2). Sample dilutions are made in Sample Diluent NS.

sELISA

Supplier Data

Sandwich ELISA - Rat Hepcidin ELISA Kit (AB322375)

Interpolated concentration of native Hepcidin was measured in duplicate at different sample concentrations. Undiluted samples are as follows : rat urine 1%. The interpolated dilution factor corrected values (to neat sample) are plotted (mean +/- SD, n=2). Sample dilutions are made in Sample Diluent NS.

sELISA

Supplier Data

Sandwich ELISA - Rat Hepcidin ELISA Kit (AB322375)

Interpolated concentration of native Hepcidin was measured in duplicate at different sample concentrations. Undiluted samples are as follows : rat serum 5%, rat plasma (citrate) 25%, and rat plasma (EDTA) 5%. The interpolated dilution factor corrected values (to neat sample) are plotted (mean +/- SD, n=2). Sample dilutions are made in Sample Diluent NS.

sELISA

Supplier Data

Sandwich ELISA - Rat Hepcidin ELISA Kit (AB322375)

Example of rat Hepcidin standard curve in Sample Diluent NS in 96-well vs. 384-well plate. Background-subtracted data values (mean +/- SD) are graphed.

sELISA

Supplier Data

Sandwich ELISA - Rat Hepcidin ELISA Kit (AB322375)

Example of rat Hepcidin standard curve in Sample Diluent NS. Background-subtracted data values (mean +/- SD) are graphed.

Key facts

Detection method

Colorimetric

Sample types

Serum, Citrate plasma, EDTA Plasma, Cell culture media, Urine

Reacts with

Rat

Assay type

Sandwich (quantitative)

Sensitivity

= 2.091 pg/mL

Range

3.906 - 250 pg/mL

Assay time

1h 30m

Assay Platform

Microplate (12 x 8 well strips)

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }

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Product details

Rat Hepcidin SimpleStep ELISA® kit is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of Hepcidin protein in Serum, Cit Plasma, EDTA Plasma, Cell culture media, Urine. Quantitate Rat Hepcidin with 2.091 pg/ml sensitivity.

SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details.

Our SimpleStep ELISA® technology provides several benefits:

-Single-wash protocol reduces assay time to 90 minutes or less

-High sensitivity, specificity and reproducibility from superior antibodies

-Fully validated in biological samples

-96-wells plate breakable into 12 x 8 wells strips, available in 10-pack (10 x 96-well plates)

-Also available in a fully validated 384-well format.

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Precision

[ { "reproducibilityType": "Intra", "sample": "Serum", "replicates": 8, "mean": null, "standardDeviation": null, "coefficientOfVariability": "= 3.9" }, { "reproducibilityType": "Inter", "sample": "Serum", "replicates": 3, "mean": null, "standardDeviation": null, "coefficientOfVariability": "= 9.2" } ]

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Recovery

[ { "sample": "Serum", "range": "96 - 99 %", "average": "= 97" }, { "sample": "Citrate plasma", "range": "81 - 84 %", "average": "= 83" }, { "sample": "Heparin Plasma", "range": "83 - 93 %", "average": "= 87" }, { "sample": "Cell culture supernatant", "range": "94 - 105 %", "average": "= 101" } ]

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What's included?

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Properties and storage information

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

+4°C

Storage information

+4°C

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Product protocols

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Target data

Liver-produced hormone that constitutes the main circulating regulator of iron absorption and distribution across tissues. Acts by promoting endocytosis and degradation of ferroportin/SLC40A1, leading to the retention of iron in iron-exporting cells and decreased flow of iron into plasma. Controls the major flows of iron into plasma : absorption of dietary iron in the intestine, recycling of iron by macrophages, which phagocytose old erythrocytes and other cells, and mobilization of stored iron from hepatocytes.. Has strong antimicrobial activity against E.coli ML35P N.cinerea and weaker against S.epidermidis, S.aureus and group b streptococcus bacteria. Active against the fungus C.albicans. No activity against P.aeruginosa.

See full target information Hamp

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websiteProtocolBooklet

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Product promise

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