Rat Hepcidin ELISA Kit is a single-wash 90-min SimpleStep ELISA® for the quantitative measurement of Rat Hepcidin in Serum, Citrate plasma, EDTA Plasma, Cell culture media, Urine samples.
Images
Key facts
- Detection method
- Colorimetric
- Sample types
- Serum, Citrate plasma, EDTA Plasma, Cell culture media, Urine
- Assay type
- Sandwich (quantitative)
- Reactive species
- Rat
- Range
- 3.906 - 250 pg/mL
- Assay time
- 1h 30m
- Sensitivity
- = 2.091 pg/mL
Reactivity data
| Application | Reactivity | Dilution info | Notes |
|---|---|---|---|
Application sELISA | Reactivity Reacts | Dilution info - | Notes - |
Target data
Function
Liver-produced hormone that constitutes the main circulating regulator of iron absorption and distribution across tissues. Acts by promoting endocytosis and degradation of ferroportin/SLC40A1, leading to the retention of iron in iron-exporting cells and decreased flow of iron into plasma. Controls the major flows of iron into plasma: absorption of dietary iron in the intestine, recycling of iron by macrophages, which phagocytose old erythrocytes and other cells, and mobilization of stored iron from hepatocytes. Has strong antimicrobial activity against E.coli ML35P N.cinerea and weaker against S.epidermidis, S.aureus and group b streptococcus bacteria. Active against the fungus C.albicans. No activity against P.aeruginosa.
Alternative names
Hepc, Hamp, Hepcidin
What's included?
Rat Hepcidin ELISA Kit is a single-wash 90-min SimpleStep ELISA® for the quantitative measurement of Rat Hepcidin in Serum, Citrate plasma, EDTA Plasma, Cell culture media, Urine samples.
Key facts
- Detection method
- Colorimetric
- Sample types
- Serum, Citrate plasma, EDTA Plasma, Cell culture media, Urine
- Assay type
- Sandwich (quantitative)
- Reactive species
- Rat
- Range
- 3.906 - 250 pg/mL
- Assay time
- 1h 30m
- Assay Platform
- Microplate (12 x 8 well strips)
- Sensitivity
- = 2.091 pg/mL
- General recovery
- 89%
Precision
Intra assay
| Sample | n | mean | SD | C.V. |
|---|---|---|---|---|
Sample Serum | n 8 | mean - | SD - | C.V. = 3.9 |
Inter assay
| Sample | n | mean | SD | C.V. |
|---|---|---|---|---|
Sample Serum | n 3 | mean - | SD - | C.V. = 9.2 |
Recovery
Sample specific recovery
| Sample type | Average % | Range |
|---|---|---|
Sample type Serum | Average % = 97 | Range 96 - 99 % |
Sample type Citrate plasma | Average % = 83 | Range 81 - 84 % |
Sample type Heparin Plasma | Average % = 87 | Range 83 - 93 % |
Sample type Cell culture supernatant | Average % = 101 | Range 94 - 105 % |
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- +4°C
- Storage information
- +4°C
Notes
Rat Hepcidin SimpleStep ELISA® kit is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of Hepcidin protein in Serum, Cit Plasma, EDTA Plasma, Cell culture media, Urine. Quantitate Rat Hepcidin with 2.091 pg/ml sensitivity.
SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details.
Our SimpleStep ELISA® technology provides several benefits:
-Single-wash protocol reduces assay time to 90 minutes or less
-High sensitivity, specificity and reproducibility from superior antibodies
-Fully validated in biological samples
-96-wells plate breakable into 12 x 8 wells strips, available in 10-pack (10 x 96-well plates)
-Also available in a fully validated 384-well format.
Product promise
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
5 product images
Sandwich ELISA - Rat Hepcidin ELISA Kit (ab322375)
Interpolated concentration of native Hepcidin was measured in duplicate at different sample concentrations in 96-well vs. 384-well plates. Undiluted samples are 5% rat serum. The interpolated dilution factor corrected values (to neat sample) are plotted (mean +/- SD, n=2). Sample dilutions are made in Sample Diluent NS.
Sandwich ELISA - Rat Hepcidin ELISA Kit (ab322375)
Example of rat Hepcidin standard curve in Sample Diluent NS in 96-well vs. 384-well plate. Background-subtracted data values (mean +/- SD) are graphed.
Sandwich ELISA - Rat Hepcidin ELISA Kit (ab322375)
Interpolated concentration of native Hepcidin was measured in duplicate at different sample concentrations. Undiluted samples are as follows: rat urine 1%. The interpolated dilution factor corrected values (to neat sample) are plotted (mean +/- SD, n=2). Sample dilutions are made in Sample Diluent NS.
Sandwich ELISA - Rat Hepcidin ELISA Kit (ab322375)
Interpolated concentration of native Hepcidin was measured in duplicate at different sample concentrations. Undiluted samples are as follows: rat serum 5%, rat plasma (citrate) 25%, and rat plasma (EDTA) 5%. The interpolated dilution factor corrected values (to neat sample) are plotted (mean +/- SD, n=2). Sample dilutions are made in Sample Diluent NS.
Sandwich ELISA - Rat Hepcidin ELISA Kit (ab322375)
Example of rat Hepcidin standard curve in Sample Diluent NS. Background-subtracted data values (mean +/- SD) are graphed.
Downloads
Product protocols
- Visit the general protocols
- Visit troubleshooting
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