AB100763

Rat ICAM1 ELISA Kit (CD54)

Name: Rat ICAM1 ELISA Kit (CD54) (ab100763) | Abcam
Brand: Abcam Limited
SKU: AB100763
Price: 780 USD
Availability: InStock

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(10 Publications)

Rat ICAM1 ELISA Kit (CD54) is a Sandwich (quantitative) ELISA for the measurement of Rat ICAM1 (CD54) in Rat in Biofluids, Cell Culture Media samples.

View Alternative Names

CD54, Icam-1, Icam1, Intercellular adhesion molecule 1, ICAM-1

2 Images

Sandwich ELISA - Rat ICAM1 ELISA Kit (CD54) (AB100763)

sELISA

Supplier Data

Sandwich ELISA - Rat ICAM1 ELISA Kit (CD54) (AB100763)

Representative Standard Curve using ab100763

sELISA

Supplier Data

Sandwich ELISA - Rat ICAM1 ELISA Kit (CD54) (AB100763)

Representative Standard Curve using ab100763

Key facts

Detection method

Colorimetric

Sample types

Plasma, Cell culture supernatant, Serum

Reacts with

Rat

Assay type

Sandwich (quantitative)

Sensitivity

< 20 pg/mL

Range

28.81 - 7000 pg/mL

Assay Platform

Microplate

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }

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Product details

Abcam's ICAM1 Rat ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of Rat ICAM- 1 in serum, plasma and cell culture supernatants.

This assay employs an antibody specific for Rat ICAM1 coated on a 96- well plate. Standards and samples are pipetted into the wells and ICAM1 present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-Rat ICAM1 antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of ICAM1 bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.

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Recovery

[ { "sample": "Cell culture supernatant", "range": "89 - 107 %", "average": "= 98.54" }, { "sample": "Serum", "range": "80 - 101 %", "average": "= 89.42" }, { "sample": "Plasma", "range": "76 - 95 %", "average": "= 85.54" } ]

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What's included?

{ "values": { "1x96Tests": { "sellingSize": "1 x 96 Tests", "publicAssetCode":"ab100763-1x96Tests", "assetComponentDetails": [ { "size":"1 x 25 mL", "name":"20X Wash Buffer", "number":"AB100763-CMP01", "productcode":"" }, { "size":"1 x 15 mL", "name":"5X Assay Diluent B", "number":"AB100763-CMP04", "productcode":"" }, { "size":"1 x 8 mL", "name":"Stop Solution", "number":"AB100763-CMP07", "productcode":"" }, { "size":"1 x 1 Unit", "name":"ICAM1 Microplate (12 x 8 wells)", "number":"AB100763-CMP05", "productcode":"" }, { "size":"1 x 200 µL", "name":"400X HRP-Streptavidin Concentrate", "number":"AB100763-CMP02", "productcode":"" }, { "size":"1 x 12 mL", "name":"TMB One-Step Substrate Reagent", "number":"AB100763-CMP08", "productcode":"" }, { "size":"2 x 1 Vial", "name":"Recombinant Rat ICAM1 Standard (lyophilized)", "number":"AB100763-CMP06", "productcode":"" }, { "size":"1 x 30 mL", "name":"Assay Diluent A", "number":"AB100763-CMP03", "productcode":"" }, { "size":"2 x 1 Vial", "name":"Biotinylated anti-Rat ICAM1", "number":"AB100763-CMP09", "productcode":"" } ] } } }

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Properties and storage information

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

-20°C

Appropriate long-term storage conditions

-20°C

Storage information

-20°C

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Product protocols

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Target data

ICAM proteins are ligands for the leukocyte adhesion protein LFA-1 (integrin alpha-L/beta-2). During leukocyte trans-endothelial migration, ICAM1 engagement promotes the assembly of endothelial apical cups through ARHGEF26/SGEF and RHOG activation (By similarity).

See full target information Icam1

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Publications (10)

Recent publications for all applications. Explore the full list and refine your search

Medicina (Kaunas, Lithuania) 59: PubMed37893548

2023

Esculeoside A Decreases Diabetic Cardiomyopathy in Streptozotocin-Treated Rats by Attenuating Oxidative Stress, Inflammation, Fibrosis, and Apoptosis: Impressive Role of Nrf2.

Applications

Unspecified application

Species

Unspecified reactive species

Jozaa Z ALTamimi,Nora A AlFaris,Ghedeir M Alshammari,Reham I Alagal,Dalal H Aljabryn,Mohammed Abdo Yahya

BMC pharmacology & toxicology 23:98 PubMed36585682

2022

Hydrogen sulfide attenuates lung injury instigated by Bisphenol-A via suppressing inflammation and oxidative stress.

Applications

Unspecified application

Species

Unspecified reactive species

Omayma A R Abo-Zaid,Fatma S M Moawed,Hend A Hassan,Enas M Moustafa

Life (Basel, Switzerland) 12: PubMed36294936

2022

Ameliorative Effect of D-Carvone against Hepatic Ischemia-Reperfusion-Induced Injury in Rats.

Applications

Unspecified application

Species

Unspecified reactive species

Maged E Mohamed,Nancy S Younis

Medical science monitor : international medical journal of experimental and clinical research 27:e928480 PubMed33931577

2021

Thrombin Aggravates Hypoxia/Reoxygenation Injury of Cardiomyocytes by Activating an Autophagy Pathway-Mediated by SIRT1.

Applications

Unspecified application

Species

Unspecified reactive species

Xiaoning Wang,Yunhe Xu,Lingbo Li,Weiwei Lu

Cellular signalling 62:109325 PubMed31132398

2019

Up-regulation of ANXA1 suppresses polymorphonuclear neutrophil infiltration and myeloperoxidase activity by activating STAT3 signaling pathway in rat models of myocardial ischemia-reperfusion injury.

Applications

Unspecified application

Species

Unspecified reactive species

Can Zhao,Beibei Zhang,Jing Jiang,Yongliang Wang,Yongquan Wu

Heliyon 5:e01359 PubMed30957042

2019

Chemical composition and protective role of (L.) C.A. Mey. subsp. against gastric ulcer induced by ethanol in rats.

Applications

Unspecified application

Species

Unspecified reactive species

Abdelgawad A Fahmi,Mariam Abdur-Rahman,Asmaa F Aboul Naser,Manal A Hamed,Howaida I Abd-Alla,Nagwa M M Shalaby,Mohamed I Nasr

Biomarkers : biochemical indicators of exposure, response, and susceptibility to chemicals 24:286-294 PubMed30512969

2018

mitigates gastric ulcer induced by ethanol in rats: role of treatment and auto healing.

Applications

Unspecified application

Species

Unspecified reactive species

Abdelgawad A Fahmi,Mariam Abdur-Rahman,Asmaa F Aboul Naser,Manal A Hamed,Howaida I Abd-Alla,Mohamed I Nasr

Molecules (Basel, Switzerland) 21: PubMed27973425

2016

Zerumbone, a Bioactive Sesquiterpene, Ameliorates Diabetes-Induced Retinal Microvascular Damage through Inhibition of Phospho-p38 Mitogen-Activated Protein Kinase and Nuclear Factor-κB Pathways.

Applications

Unspecified application

Species

Unspecified reactive species

Wayne Young Liu,Thing-Fong Tzeng,I-Min Liu

Genetics and molecular research : GMR 15: PubMed27323014

2016

Effect of ozone on vascular endothelial growth factor (VEGF) and related inflammatory cytokines in rats with diabetic retinopathy.

Applications

Unspecified application

Species

Unspecified reactive species

T Y Xie,W Yan,J Lou,X Y Chen

Nutrients 7:7821-41 PubMed26389948

2015

Consumption of Polyphenol-Rich Zingiber Zerumbet Rhizome Extracts Protects against the Breakdown of the Blood-Retinal Barrier and Retinal Inflammation Induced by Diabetes.

Applications

Unspecified application

Species

Unspecified reactive species

Thing-Fong Tzeng,Tang-Yao Hong,Yu-Cheng Tzeng,Shorong-Shii Liou,I-Min Liu

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Rat ICAM1 ELISA Kit (CD54)

Sandwich ELISA - Rat ICAM1 ELISA Kit (CD54) (AB100763)

Sandwich ELISA - Rat ICAM1 ELISA Kit (CD54) (AB100763)

Key facts

Detection method

Sample types

Reacts with

Assay type

Sensitivity

Range

Assay Platform

Reactivity data

Product details

Recovery

What's included?

Properties and storage information

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Storage information

Product protocols

Target data

Publications (10)

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Product promise