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AB239425

Rat IFN gamma ELISA Kit

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(8 Publications)

Rat IFN gamma ELISA Kit is a single-wash 90-min Simplestep used to quantify Rat IFN gamma with a sensitivity of 1.1 pg/ml. The assay uses a simple mix-wash-read protocol with just one incubation and one wash step.

- Colorimetric Sandwich ELISA - 450 nm readout : works on any standard plate reader
- Design your own immunoassay: we also offer the conjugation-ready antibody pair
- Cited in over 5 citations

View Alternative Names

Interferon gamma, IFN-gamma, Ifng

5 Images
ELISA - Rat IFN gamma ELISA Kit (AB239425)
  • ELISA

Supplier Data

ELISA - Rat IFN gamma ELISA Kit (AB239425)
Sandwich ELISA - Rat IFN gamma ELISA Kit (AB239425)
  • sELISA

Unknown

Sandwich ELISA - Rat IFN gamma ELISA Kit (AB239425)

Interpolated concentrations of native IFN gamma in treated and untreated rat splenocyte cell culture supernatant samples.

Rat splenocyte cells were cultured in the presence (treated) or absence (untreated) of 5 μg/mL of Concanavalin A for 48 hours. The concentrations of IFN gamma were measured in duplicates, interpolated from the IFN gamma standard curves and corrected for sample dilution. Undiluted samples are as follows : treated splenocyte supernatant 10% and untreated splenocyte supernatant 100%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean IFN gamma concentration was determined to be 4074 pg/mL in neat treated splenocyte supernatant, and 17.27 pg/mL in neat untreated splenocyte supernatant.

Sandwich ELISA - Rat IFN gamma ELISA Kit (AB239425)
  • sELISA

Unknown

Sandwich ELISA - Rat IFN gamma ELISA Kit (AB239425)

Rat IFN gamma Standard Curve Comparison. Standard Curve comparison between Rat IFN gamma SimpleStep ELISA kit and traditional ELISA kit from leading competitor. SimpleStep ELISA kit shows increased sensitivity.

Sandwich ELISA - Rat IFN gamma ELISA Kit (AB239425)
  • sELISA

Unknown

Sandwich ELISA - Rat IFN gamma ELISA Kit (AB239425)

Example of rat IFN gamma standard curve in Sample Diluent 75BP. The IFN gamma standard curve was prepared as described in Section 10. Raw data values are shown in the table. Background-subtracted data values (mean +/- SD) are graphed.

Sandwich ELISA - Rat IFN gamma ELISA Kit (AB239425)
  • sELISA

Unknown

Sandwich ELISA - Rat IFN gamma ELISA Kit (AB239425)

Example of rat IFN gamma standard curve in Sample Diluent NS. The IFN gamma standard curve was prepared as described in Section 10. Raw data values are shown in the table. Background-subtracted data values (mean +/- SD) are graphed.

Key facts

Detection method

Colorimetric

Sample types

Heparin Plasma, Citrate plasma, Cell culture supernatant, Serum, EDTA Plasma

Reacts with

Rat

Assay type

Sandwich (quantitative)

Sensitivity

= 1.1 pg/mL

Range

7 - 500 pg/mL

Assay time

1h 30m

Assay Platform

Pre-coated microplate (12 x 8 well strips)

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Product details

Rat IFN gamma ELISA Kit ab239425 is a rapid single-wash 90-min Sandwich ELISA to measure Rat IFN gamma in cell culture supernatant, citrate plasma, EDTA plasma, heparin plasma, serum. This SimpleStep sensitivity is 1.1 pg/mL.

How the assay works

Rat IFN gamma SimpleStep ELISA®employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details.

Assay Specificity

Our SimpleStep ELISA® kits use recombinant monoclonal antibodies rigorously validated to ensure the highest level of consistency and reproducibility, improved sensitivity and specificity and ease of scalability and security of supply.
Please refer to our protocol booklet for more details.

Rat IFN gamma ELISA Kit ab239425 protocol summary

1. Mix: add samples/standards to the wells together with the capture and detector antibody cocktail. Incubate 1 hr at room temperature
2. Wash
3. Add TMB development solution - incubate for 10 min
4. Add Stop solution
5. Read the results on a plate reader at 450 nm

Design your own immunoassay

We offer the antibody pair used in this kit in a BSA and Azide-free format, ready for conjugation:

- Anti-Interferon gamma antibody [EPR20701-29] - BSA and Azide free (Capture) ab245082
- Anti-Interferon gamma antibody [EPR20701-39] - BSA and Azide free (Detector) ab245082
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Precision

[ { "reproducibilityType": "Inter", "sample": "Splenocytes", "replicates": 3, "mean": null, "standardDeviation": null, "coefficientOfVariability": "5" }, { "reproducibilityType": "Intra", "sample": "Splenocytes", "replicates": 8, "mean": null, "standardDeviation": null, "coefficientOfVariability": "3.6" } ]
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Recovery

[ { "sample": "Cell culture supernatant", "range": "93 - 106 %", "average": "= 100" }, { "sample": "Serum", "range": "93 - 104 %", "average": "= 97" }, { "sample": "EDTA Plasma", "range": "120 - 127 %", "average": "= 122" }, { "sample": "Heparin Plasma", "range": "94 - 96 %", "average": "= 95" }, { "sample": "Citrate plasma", "range": "107 - 110 %", "average": "= 109" }, { "sample": "Cell culture media", "range": "91 - 97 %", "average": "= 95" } ]
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What's included?

{ "values": { "1x96Tests": { "sellingSize": "1 x 96 Tests", "publicAssetCode":"ab239425-1x96Tests", "assetComponentDetails": [ { "size":"1 x 20 mL", "name":"10X Wash Buffer PT (ab206977)", "number":"AB239425-CMP11", "productcode":"" }, { "size":"1 x 12 mL", "name":"Stop Solution", "number":"AB239425-CMP05", "productcode":"" }, { "size":"1 x 50 mL", "name":"Sample Diluent NS (ab193972)", "number":"AB239425-CMP03", "productcode":"" }, { "size":"1 x 12 mL", "name":"TMB Development Solution", "number":"AB239425-CMP06", "productcode":"" }, { "size":"1 x 1 Unit", "name":"Plate Seals", "number":"AB239425-CMP10", "productcode":"" }, { "size":"1 x 600 µL", "name":"10X Rat IFN gamma Capture Antibody", "number":"AB239425-CMP08", "productcode":"" }, { "size":"1 x 20 mL", "name":"Sample Diluent 75BP", "number":"AB239425-CMP02", "productcode":"" }, { "size":"1 x 6 mL", "name":"Antibody Diluent 4BR", "number":"AB239425-CMP01", "productcode":"" }, { "size":"2 x 1 Vial", "name":"Rat IFN gamma Lyophilized Recombinant Protein", "number":"AB239425-CMP07", "productcode":"" }, { "size":"1 x 600 µL", "name":"10X Rat IFN gamma Detector Antibody", "number":"AB239425-CMP09", "productcode":"" }, { "size":"1 x 1 Unit", "name":"SimpleStep Pre-Coated 96-Well Microplate (ab206978)", "number":"AB239425-CMP04", "productcode":"" } ] } } }
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Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
+4°C
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Interferon gamma (IFN-?) also known as type II interferon is a cytokine that plays an important role in immune response. IFN-? has a molecular weight of about 17 kDa and is produced by T cells and natural killer (NK) cells. IFN-? binds to the interferon gamma receptor initiating a signaling cascade that activates various genes involved in immune functions. It is expressed mainly in activated immune cells within lymphoid tissues and inflamed sites during immune responses.
Biological function summary

This cytokine is significant in promoting macrophage activation enhancing the antigen presentation process and boosting the antimicrobial activity of phagocytes. IFN-? is not part of a larger protein complex but works as a homodimer in signal transduction. Its production heightens the Th1 immune response by stimulating the differentiation of naпve T cells into Th1 cells which is essential for effective cellular immunity.

Pathways

IFN-? is integrally involved in the JAK-STAT signaling pathway alongside another critical cytokine Interleukin-12. This pathway further amplifies the immune response by regulating the expression of genes associated with cellular defense mechanisms. IFN-? also interacts with the NF-kB pathway influencing inflammation and the activation of further immune responses. These interactions show a network of cooperativity with proteins like STAT1 and NF-kB essential for executing its biological roles.

IFN-? is linked to autoimmune diseases such as rheumatoid arthritis and multiple sclerosis where its elevated levels can exacerbate inflammatory processes. It connects to other proteins like TNF-alpha in promoting the inflammatory cascade. Moreover lower levels of IFN-? are associated with a heightened risk of infections like tuberculosis demonstrating its vital role in pathogen defense. Therefore understanding IFN-? and its interactions can be key in developing therapeutic approaches against these conditions.
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Product protocols

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Target data

Type II interferon produced by immune cells such as T-cells and NK cells that plays crucial roles in antimicrobial, antiviral, and antitumor responses by activating effector immune cells and enhancing antigen presentation. Primarily signals through the JAK-STAT pathway after interaction with its receptor IFNGR1 to affect gene regulation. Upon IFNG binding, IFNGR1 intracellular domain opens out to allow association of downstream signaling components JAK2, JAK1 and STAT1, leading to STAT1 activation, nuclear translocation and transcription of IFNG-regulated genes. Many of the induced genes are transcription factors such as IRF1 that are able to further drive regulation of a next wave of transcription. Plays a role in class I antigen presentation pathway by inducing a replacement of catalytic proteasome subunits with immunoproteasome subunits. In turn, increases the quantity, quality, and repertoire of peptides for class I MHC loading. Increases the efficiency of peptide generation also by inducing the expression of activator PA28 that associates with the proteasome and alters its proteolytic cleavage preference. Up-regulates as well MHC II complexes on the cell surface by promoting expression of several key molecules such as cathepsins B/CTSB, H/CTSH, and L/CTSL (By similarity). Participates in the regulation of hematopoietic stem cells during development and under homeostatic conditions by affecting their development, quiescence, and differentiation (By similarity).
See full target information Ifng
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Publications (8)

Recent publications for all applications. Explore the full list and refine your search

Allergologia et immunopathologia 53:88-97 PubMed40342118

2025

Dieckol ameliorates inflammatory response via inhibition of CHI3L1 expression in collagen-induced arthritis rats.

Applications

Unspecified application

Species

Unspecified reactive species

Weijiang Wang,Huijie Li

Pharmaceuticals (Basel, Switzerland) 18: PubMed40283939

2025

Immune Basis of Therapeutic Effects of L. Polysaccharide on Chronic Peptic Ulcer Healing.

Applications

Unspecified application

Species

Unspecified reactive species

Evgenii Generalov,Denis Laryushkin,Kristina Kritskaya,Nina Kulchenko,Arkady Sinitsyn,Leonid Yakovenko,Liubov Generalova,Nikolay Belostotsky

Heliyon 10:e37845 PubMed39328544

2024

Immune-enhancing effect of fermented soybean food, on cyclophosphamide-treated immunosuppressed rat.

Applications

Unspecified application

Species

Unspecified reactive species

Hak Yong Lee,Young Mi Park,Dong Yeop Shin,Hai Min Hwang,Han Na Jeong,Hyo Yeon Park,Hee-Jong Yang,Gwang Su Ha,Myeong Seon Ryu,Ji Won Seo,Do-Youn Jeong,Jun Sang Bae,Byeong Soo Kim,Jae Gon Kim

BMC psychiatry 22:419 PubMed35733107

2022

Alterations of monoamine neurotransmitters, HPA-axis hormones, and inflammation cytokines in reserpine-induced hyperalgesia and depression comorbidity rat model.

Applications

Unspecified application

Species

Unspecified reactive species

Jingjie Zhao,Wei Shi,Yujia Lu,Xuesong Gao,Anna Wang,Shan Zhang,Yi Du,Yongzhi Wang,Li Li

BMC neuroscience 23:34 PubMed35676653

2022

The functional mechanisms of synchronizing royal jelly consumption and physical activity on rat with multiple sclerosis-like behaviors hallmarks based on bioinformatics analysis, and experimental survey.

Applications

Unspecified application

Species

Unspecified reactive species

Maryam Lohrasbi,Farzaneh Taghian,Khosro Jalali Dehkordi,Seyed Ali Hosseini

Journal of immunology research 2022:7200379 PubMed35465352

2022

Oral S2-Ag85 DNA Vaccine Activated Intestinal Cell dsDNA and RNA Sensors to Promote the Presentation of Intestinal Antigen.

Applications

Unspecified application

Species

Unspecified reactive species

Dang Sheng,Sui Xin,Wu Dongxing,Wen Shubo,Song Yang,Chen Zeliang,Zhai Jingbo

Evidence-based complementary and alternative medicine : eCAM 2021:5518750 PubMed34745289

2021

Unveiling the Mechanism of Principal Drugs of Lianpu Drink on Chronic Gastritis by Network Pharmacology.

Applications

Unspecified application

Species

Unspecified reactive species

Shuhan Zhou,Xiaohui Xu,Jiangqin Zeng,Zhiyi Liu,Miao Wang,Wenliang Lv

Oxidative medicine and cellular longevity 2020:2394704 PubMed32351668

2020

Chitoheptaose Promotes Heart Rehabilitation in a Rat Myocarditis Model by Improving Antioxidant, Anti-Inflammatory, and Antiapoptotic Properties.

Applications

Unspecified application

Species

Unspecified reactive species

Qini Zhao,Liquan Yin,Lirong Zhang,Dongli Jiang,Long Liu,Hong Ji
View all publications
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