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AB46107

Rat IFN gamma ELISA Kit

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(16 Publications)

Rat IFN gamma ELISA Kit is a Sandwich (quantitative) ELISA for the measurement of Rat IFN gamma in Rat in Cell Culture Media, Biofluids samples.

View Alternative Names

Interferon gamma, IFN-gamma, Ifng

1 Images
Sandwich ELISA - Rat IFN gamma ELISA Kit (AB46107)
  • sELISA

Unknown

Sandwich ELISA - Rat IFN gamma ELISA Kit (AB46107)

Representative Standard Curve using ab46107

Key facts

Detection method

Colorimetric

Sample types

Cell culture supernatant, Serum

Reacts with

Rat

Assay type

Sandwich (quantitative)

Sensitivity

< 10 pg/mL

Range

31.25 - 1000 pg/mL

Assay time

3h 45m

Assay Platform

Microplate

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Product details

Abcam's IFN gamma Rat in vitro ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for the quantitative measurement of IFN gamma in cell culture supernatants, buffered solutions and plasma.

A monoclonal antibody specific for IFN gamma has been coated onto the wells of the microtiter strips provided. Samples, including standards of known IFN gamma concentrations, control specimens or unknowns are pipetted into these wells. During the first incubation, the standards or samples and a biotinylated monoclonal antibody specific for IFN gamma are simultaneously incubated. After washing, the enzyme Streptavidin-HRP, that binds the biotinylated antibody is added, incubated and washed. A TMB substrate solution is added which acts on the bound enzyme to induce a colored reaction product. The intensity of this colored product is directly proportional to the concentration of IFN gamma present in the samples.

This kit will recognize both endogenous and recombinant rat IFN gamma

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Precision

[ { "reproducibilityType": "Inter", "sample": "Sample A", "replicates": 6, "mean": "148.2 pg/mL", "standardDeviation": "6.6", "coefficientOfVariability": "4.5" }, { "reproducibilityType": "Inter", "sample": "Sample B", "replicates": 6, "mean": "560.7 pg/mL", "standardDeviation": "29.5", "coefficientOfVariability": "5.2" }, { "reproducibilityType": "Intra", "sample": "Sample A", "replicates": 19, "mean": "365.1 pg/mL", "standardDeviation": "12.7", "coefficientOfVariability": "2.9" }, { "reproducibilityType": "Intra", "sample": "Sample B", "replicates": 20, "mean": "554.6 pg/mL", "standardDeviation": "16.4", "coefficientOfVariability": "3.4" } ]
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What's included?

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Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
+4°C
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Interferon gamma (IFN-γ) also known as type II interferon is a cytokine that plays an important role in immune response. IFN-γ has a molecular weight of about 17 kDa and is produced by T cells and natural killer (NK) cells. IFN-γ binds to the interferon gamma receptor initiating a signaling cascade that activates various genes involved in immune functions. It is expressed mainly in activated immune cells within lymphoid tissues and inflamed sites during immune responses.
Biological function summary

This cytokine is significant in promoting macrophage activation enhancing the antigen presentation process and boosting the antimicrobial activity of phagocytes. IFN-γ is not part of a larger protein complex but works as a homodimer in signal transduction. Its production heightens the Th1 immune response by stimulating the differentiation of naïve T cells into Th1 cells which is essential for effective cellular immunity.

Pathways

IFN-γ is integrally involved in the JAK-STAT signaling pathway alongside another critical cytokine Interleukin-12. This pathway further amplifies the immune response by regulating the expression of genes associated with cellular defense mechanisms. IFN-γ also interacts with the NF-kB pathway influencing inflammation and the activation of further immune responses. These interactions show a network of cooperativity with proteins like STAT1 and NF-kB essential for executing its biological roles.

IFN-γ is linked to autoimmune diseases such as rheumatoid arthritis and multiple sclerosis where its elevated levels can exacerbate inflammatory processes. It connects to other proteins like TNF-alpha in promoting the inflammatory cascade. Moreover lower levels of IFN-γ are associated with a heightened risk of infections like tuberculosis demonstrating its vital role in pathogen defense. Therefore understanding IFN-γ and its interactions can be key in developing therapeutic approaches against these conditions.
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Product protocols

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Target data

Type II interferon produced by immune cells such as T-cells and NK cells that plays crucial roles in antimicrobial, antiviral, and antitumor responses by activating effector immune cells and enhancing antigen presentation. Primarily signals through the JAK-STAT pathway after interaction with its receptor IFNGR1 to affect gene regulation. Upon IFNG binding, IFNGR1 intracellular domain opens out to allow association of downstream signaling components JAK2, JAK1 and STAT1, leading to STAT1 activation, nuclear translocation and transcription of IFNG-regulated genes. Many of the induced genes are transcription factors such as IRF1 that are able to further drive regulation of a next wave of transcription. Plays a role in class I antigen presentation pathway by inducing a replacement of catalytic proteasome subunits with immunoproteasome subunits. In turn, increases the quantity, quality, and repertoire of peptides for class I MHC loading. Increases the efficiency of peptide generation also by inducing the expression of activator PA28 that associates with the proteasome and alters its proteolytic cleavage preference. Up-regulates as well MHC II complexes on the cell surface by promoting expression of several key molecules such as cathepsins B/CTSB, H/CTSH, and L/CTSL (By similarity). Participates in the regulation of hematopoietic stem cells during development and under homeostatic conditions by affecting their development, quiescence, and differentiation (By similarity).
See full target information Ifng
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Publications (16)

Recent publications for all applications. Explore the full list and refine your search

International journal of molecular sciences 24: PubMed37762060

2023

Extract as a Promising Supplemental Agent for Hepatic and Renal Complication-Associated Type 2 Diabetes (In Vivo and In Silico-Based Studies).

Applications

Unspecified application

Species

Unspecified reactive species

Eman Fawzy El Azab,Saleha Y M Alakilli,Abdulrahman M Saleh,Hassan H Alhassan,Hamad H Alanazi,Heba Bassiony Ghanem,Sara Osman Yousif,Heba Abu Alrub,Nahla Anber,Elyasa Mustafa Elfaki,Alneil Hamza,Shaymaa Abdulmalek

Current issues in molecular biology 45:1183-1196 PubMed36826023

2023

Myrrh Essential Oil Mitigates Renal Ischemia/Reperfusion-Induced Injury.

Applications

Unspecified application

Species

Unspecified reactive species

Nancy S Younis

Antioxidants (Basel, Switzerland) 11: PubMed36009287

2022

Renal Ischemia/Reperfusion Mitigation via Geraniol: The Role of Nrf-2/HO-1/NQO-1 and TLR2,4/MYD88/NFκB Pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Maged E Mohamed,Mohammad A Elmorsy,Nancy S Younis

Antioxidants (Basel, Switzerland) 11: PubMed35326185

2022

The Protective Effect of Anethole against Renal Ischemia/Reperfusion: The Role of the TLR2,4/MYD88/NFκB Pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Maged Elsayed Mohamed,Mahmoud Kandeel,Hany M Abd El-Lateef,Hossam S El-Beltagi,Nancy S Younis

European journal of clinical microbiology & infectious diseases : official publication of the European Society of Clinical Microbiology 40:1623-1631 PubMed33666790

2021

Therapeutic efficacy of chitosan nanoparticles loaded with BCG-polysaccharide nucleic acid and ovalbumin on airway inflammation in asthmatic mice.

Applications

Unspecified application

Species

Unspecified reactive species

Wentao Yang,Zhaohui Dong,Yujing Li,Yingying Zhang,Huanqin Fu,Yanping Xie

IUBMB life 72:440-451 PubMed32031750

2020

Long noncoding RNA colorectal neoplasia differentially expressed alleviates sepsis-induced liver injury via regulating miR-126-5p.

Applications

Unspecified application

Species

Unspecified reactive species

Yang Li,Jianfeng Song,Zichen Xie,Mei Liu,Keyu Sun

International journal of molecular medicine 45:1150-1162 PubMed32124940

2020

HDAC inhibitor sodium butyrate prevents allergic rhinitis and alters lncRNA and mRNA expression profiles in the nasal mucosa of mice.

Applications

Unspecified application

Species

Unspecified reactive species

Jie Wang,Mu Cui,Fei Sun,Ke Zhou,Bei Fan,Jian-Hua Qiu,Fu-Quan Chen

BMC complementary and alternative medicine 19:349 PubMed31801507

2019

Immunomodulatory and anti-inflammatory effects of hydro-ethanolic extract of Ocimum basilicum leaves and its effect on lung pathological changes in an ovalbumin-induced rat model of asthma.

Applications

Unspecified application

Species

Unspecified reactive species

Naima Eftekhar,Ali Moghimi,Nema Mohammadian Roshan,Saeideh Saadat,Mohammad Hossein Boskabady

European surgical research. Europaische chirurgisc 60:196-207 PubMed31694021

2019

The Mechanism of Electroacupuncture at Zusanli Promotes Macrophage Polarization during the Fibrotic Process in Contused Skeletal Muscle.

Applications

Unspecified application

Species

Unspecified reactive species

Mingyang Yan,Rongguo Wang,Shouyao Liu,Ying Chen,Peng Lin,Tengqi Li,Yunting Wang

Life sciences 231:116589 PubMed31226416

2019

Cytoprotective effects of galacto-oligosaccharides on colon epithelial cells via up-regulating miR-19b.

Applications

Unspecified application

Species

Unspecified reactive species

Jinwei Sun,Wenxing Liang,Xiaofeng Yang,Qiming Li,Guofang Zhang
View all publications
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