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AB213902

Rat IGF-1 ELISA Kit

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(6 Publications)

Rat IGF-1 ELISA Kit is a Sandwich ELISA for the measurement of Rat IGF-1 in Rat in Cell/Tissue Extracts, Cell Culture Media, Biofluids samples.

View Alternative Names

Igf-1, Igf1, Insulin-like growth factor 1, Insulin-like growth factor I, Somatomedin, IGF-I

1 Images
Sandwich ELISA - Rat IGF-1 ELISA Kit (AB213902)
  • sELISA

Supplier Data

Sandwich ELISA - Rat IGF-1 ELISA Kit (AB213902)

Rat IGF-1 ELISA Kit (ab213902) Standard Curve.

Key facts

Detection method

Colorimetric

Sample types

Cell Lysate, Cell culture supernatant, EDTA Plasma, Heparin Plasma, Serum

Reacts with

Rat

Assay type

Sandwich

Results type

Quantitative

Sensitivity

< 5 pg/mL

Range

62.5 - 4000 pg/mL

Assay time

3h 30m

Assay Platform

Pre-coated microplate (12 x 8 well strips)

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Product details

The Rat IGF-1 Enzyme-Linked Immunosorbent Assay (ELISA) kit (ab213902) is designed for the quantitative measurement of Rat IGF-1 in cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA).

The ELISA kit is based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from mouse specific for IGF-1 has been pre-coated onto 96-well plates. Standards and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for IGF-1 is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex is added and unbound conjugates are washed away with PBS or TBS buffer. HRP substrate TMB is used to visualize HRP enzymatic reaction. TMB is catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the Rat IGF-1 amount of sample captured in plate.

Insulin-like growth factor 1 (IGF-1) that was once called somatomedin C, is a polypeptide protein hormone similar in molecular structure to insulin. It plays an important role in childhood growth and continues to have anabolic effects in adults. Human IGF1 is a single chain 70-amino acid polypeptide cross-linked by 3 disulfide bridges, with a calculated molecular mass of 7.6 kD. The IGF1 gene, mapped on 12q22-q24.1, contains 5 exons. Exons 1-4 encode the 195-amino acid precursor (IGF1B), and exons 1, 2, 3, and 5 encode the 153-residue peptide (IGF1A). The structure of IGF1 resembles that of IGF2. And the IGF1 and IGF2 genes have complex structures with multiple promoters. The expression of both genes is regulated at the levels of transcription, RNA processing, and translation. IGF-1 is produced primarily by the liver as an endocrine hormone as well as in target tissues in a paracrine/autocrine fashion. Moreover, approximately 98% of IGF-1 is always bound to one of 6 binding proteins (IGF-BP). Furthermore, IGF-1 is one of the most potent natural activators of the AKT signaling pathway, a stimulator of cell growth and multiplication and a potent inhibitor of programmed cell death.

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Precision

[ { "reproducibilityType": "Inter", "sample": "Sample 2", "replicates": 24, "mean": "764 pg/mL", "standardDeviation": "69.52", "coefficientOfVariability": "= 9.1" }, { "reproducibilityType": "Inter", "sample": "Sample 1", "replicates": 24, "mean": "186 pg/mL", "standardDeviation": "11.16", "coefficientOfVariability": "= 6" }, { "reproducibilityType": "Intra", "sample": "Sample 1", "replicates": 16, "mean": "185 pg/mL", "standardDeviation": "9.99", "coefficientOfVariability": "= 5.4" }, { "reproducibilityType": "Inter", "sample": "Sample 3", "replicates": 24, "mean": "1861 pg/mL", "standardDeviation": "94.91", "coefficientOfVariability": "= 5.1" }, { "reproducibilityType": "Intra", "sample": "Sample 2", "replicates": 16, "mean": "754 pg/mL", "standardDeviation": "56.55", "coefficientOfVariability": "= 7.5" }, { "reproducibilityType": "Intra", "sample": "Sample 3", "replicates": 16, "mean": "1874 pg/mL", "standardDeviation": "80.58", "coefficientOfVariability": "= 4.3" } ]
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What's included?

{ "values": { "1x96Tests": { "sellingSize": "1 x 96 Tests", "publicAssetCode":"ab213902-1x96Tests", "assetComponentDetails": [ { "size":"1 x 100 µL", "name":"Avidin-Biotin-Peroxidase Complex (ABC)", "number":"AB213902-CMP10", "productcode":"" }, { "size":"1 x 100 µL", "name":"Biotinylated anti- Rat IGF-1 antibody", "number":"AB213902-CMP09", "productcode":"" }, { "size":"1 x 1 Unit", "name":"Anti-rat IGF-1 coated Microplate (12 x 8 wells)", "number":"AB213902-CMP08", "productcode":"" }, { "size":"1 x 10 mL", "name":"TMB Color Developing Agent", "number":"AB213902-CMP04", "productcode":"" }, { "size":"1 x 12 mL", "name":"Antibody Diluent Buffer", "number":"AB213902-CMP07", "productcode":"" }, { "size":"1 x 30 mL", "name":"Sample Diluent Buffer", "number":"AB213902-CMP02", "productcode":"" }, { "size":"4 x 1 Unit", "name":"Adhesive Plate Seal", "number":"AB213902-CMP06", "productcode":"" }, { "size":"2 x 1 Vial", "name":"Lyophilized recombinant Rat IGF-1 standard", "number":"AB213902-CMP01", "productcode":"" }, { "size":"1 x 10 mL", "name":"TMB Stop Solution", "number":"AB213902-CMP05", "productcode":"" }, { "size":"1 x 20 mL", "name":"Wash Buffer (25X)", "number":"AB213902-CMP11", "productcode":"" }, { "size":"1 x 12 mL", "name":"ABC Diluent Buffer", "number":"AB213902-CMP03", "productcode":"" } ] } } }
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Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
-20°C
Appropriate long-term storage conditions
-20°C
Storage information
-20°C
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

The insulin-like growth factor 1 or IGF1 is a protein hormone that plays an important mechanical role in growth and development. Alternative names for IGF1 include somatomedin C. This protein has a mass of approximately 7.6 kDa and is mainly produced by the liver. IGF1 is expressed in a range of tissues including muscle bone and cartilage reflecting its importance in various physiological processes. Its production is stimulated by growth hormone and it acts in an autocrine and paracrine manner to exert its effects.
Biological function summary

IGF1 stimulates growth and proliferation of cells most notably impacting the development of the skeletal system and the regulation of apoptosis. It is not part of a larger protein complex but it interacts closely with IGF1 receptor to execute its functions. By binding to this receptor IGF1 activates intracellular signaling cascades that promote anabolic effects which include increased uptake of glucose and amino acids contributing to muscle and tissue growth.

Pathways

IGF1 operates within the PI3K-Akt and MAPK signaling pathways which are significant for cell survival and proliferation. Its interaction with proteins like IGF binding protein 3 (IGFBP3) modulates its availability and activity in the bloodstream. These pathways are critical for mediating the effects of growth hormone highlighting the role of IGF1 in systemic growth and metabolism regulation.

Alterations in IGF1 levels associate with growth abnormalities such as Laron syndrome and acromegaly. Laron syndrome results from the body's inability to use IGF1 properly due to receptor defects while acromegaly occurs from excessive IGF1 production often due to pituitary tumors. Furthermore aberrant IGF1 signaling connects to cancer development where it can drive tumor growth and metastasis. Drugs targeting IGF1 and its pathway components continue to be researched for therapeutic potential in these conditions.
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Product protocols

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Target data

The insulin-like growth factors, isolated from plasma, are structurally and functionally related to insulin but have a much higher growth-promoting activity. May be a physiological regulator of [1-14C]-2-deoxy-D-glucose (2DG) transport and glycogen synthesis in osteoblasts. Stimulates glucose transport in bone-derived osteoblastic (PyMS) cells and is effective at much lower concentrations than insulin, not only regarding glycogen and DNA synthesis but also with regard to enhancing glucose uptake. May play a role in synapse maturation. Ca(2+)-dependent exocytosis of IGF1 is required for sensory perception of smell in the olfactory bulb. Acts as a ligand for IGF1R. Binds to the alpha subunit of IGF1R, leading to the activation of the intrinsic tyrosine kinase activity which autophosphorylates tyrosine residues in the beta subunit thus initiating a cascade of down-stream signaling events leading to activation of the PI3K-AKT/PKB and the Ras-MAPK pathways. Binds to integrins ITGAV : ITGB3 and ITGA6 : ITGB4. Its binding to integrins and subsequent ternary complex formation with integrins and IGFR1 are essential for IGF1 signaling. Induces the phosphorylation and activation of IGFR1, MAPK3/ERK1, MAPK1/ERK2 and AKT1 (By similarity). As part of the MAPK/ERK signaling pathway, acts as a negative regulator of apoptosis in cardiomyocytes via promotion of STUB1/CHIP-mediated ubiquitination and degradation of ICER-type isoforms of CREM (PubMed : 20724525).
See full target information Igf1
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Publications (6)

Recent publications for all applications. Explore the full list and refine your search

PloS one 19:e0301883 PubMed39292699

2024

PDE5 inhibitor potentially improves polyuria and bladder storage and voiding dysfunctions in type 2 diabetic rats.

Applications

Unspecified application

Species

Unspecified reactive species

Takafumi Kabuto,So Inamura,Hisato Kobayashi,Xinmin Zha,Keiko Nagase,Minekatsu Taga,Masaya Seki,Nobuki Tanaka,Yoshinaga Okumura,Osamu Yokoyama,Naoki Terada

Cells 12: PubMed38067139

2023

Repeated Intravenous Administration of Human Neural Stem Cells Producing Choline Acetyltransferase Exerts Anti-Aging Effects in Male F344 Rats.

Applications

Unspecified application

Species

Unspecified reactive species

Jangbeen Kyung,Dajeong Kim,Kyungha Shin,Dongsun Park,Soon-Cheol Hong,Tae Myoung Kim,Ehn-Kyoung Choi,Yun-Bae Kim

Stem cell reports 17:276-288 PubMed35030322

2022

Physical exercise promotes integration of grafted cells and functional recovery in an acute stroke rat model.

Applications

Unspecified application

Species

Unspecified reactive species

Rui Wu,Yiping Guo,Liying Zhang,Haiqing Zheng,Xi Chen,Mingyue Li,Qi Xing,Wenhao Huang,Zhenghui Su,Di Zhang,Xiaofen Zhong,Guangjin Pan,Xiquan Hu

Food & nutrition research 65: PubMed34650394

2021

Chronic liquid fructose supplementation does not cause liver tumorigenesis but elicits clear sex differences in the metabolic response in Sprague-Dawley rats.

Applications

Unspecified application

Species

Unspecified reactive species

Nuria Roglans,Miguel Baena,Gemma Sangüesa,Ana Magdalena Velázquez,Christian Griñán-Ferré,Mercè Pallàs,Rosa María Sánchez,Marta Alegret,Juan Carlos Laguna

Archives of toxicology 94:389-399 PubMed31820026

2019

Skeletal toxicity resulting from exposure of growing male rats to coplanar PCB 126 is associated with disruption of calcium homeostasis and the GH-IGF-1 axis and direct effects on bone formation.

Applications

Unspecified application

Species

Unspecified reactive species

Martin J Ronis,James Watt,Casey F Pulliam,Ashlee E Williams,Alexander W Alund,Ezazul Haque,Gopi S Gadupudi,Larry W Robertson

International journal of molecular medicine 40:1860-1868 PubMed29039467

2017

Insulin-like growth factor binding protein-6 released from human mesenchymal stem cells confers neuronal protection through IGF-1R-mediated signaling.

Applications

Unspecified application

Species

Unspecified reactive species

Hyo-Jin Jeon,Jihye Park,Joo-Hyun Shin,Mi-Sook Chang
View all publications
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