Rat IGF-1 ELISA Kit is a Sandwich (quantitative) ELISA kit for the measurement of Rat IGF-1 in Rat in Cell Lysate, Cell culture supernatant, EDTA Plasma, Heparin Plasma, Serum samples.
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- Cells 12:2023Repeated Intravenous Administration of Human Neural Stem Cells Producing Choline Acetyltransferase Exerts Anti-Aging Effects in Male F344 Rats.Applications:Unspecified applicationReactive species:Unspecified reactive speciesJangbeen Kyung,Dajeong Kim,Kyungha Shin,Dongsun Park,Soon-Cheol Hong,Tae Myoung Kim,Ehn-Kyoung Choi,Yun-Bae KimPubMed 38067139
- Stem cell reports 17:276-2882022Physical exercise promotes integration of grafted cells and functional recovery in an acute stroke rat model.Applications:Unspecified applicationReactive species:Unspecified reactive speciesRui Wu,Yiping Guo,Liying Zhang,Haiqing Zheng,Xi Chen,Mingyue Li,Qi Xing,Wenhao Huang,Zhenghui Su,Di Zhang,Xiaofen Zhong,Guangjin Pan,Xiquan HuPubMed 35030322
- Food & nutrition research 65:2021Chronic liquid fructose supplementation does not cause liver tumorigenesis but elicits clear sex differences in the metabolic response in Sprague-Dawley rats.Applications:Unspecified applicationReactive species:Unspecified reactive speciesNuria Roglans,Miguel Baena,Gemma Sangüesa,Ana Magdalena Velázquez,Christian Griñán-Ferré,Mercè Pallàs,Rosa María Sánchez,Marta Alegret,Juan Carlos LagunaPubMed 34650394
- Archives of toxicology 94:389-3992019Skeletal toxicity resulting from exposure of growing male rats to coplanar PCB 126 is associated with disruption of calcium homeostasis and the GH-IGF-1 axis and direct effects on bone formation.Applications:Unspecified applicationReactive species:Unspecified reactive speciesMartin J Ronis,James Watt,Casey F Pulliam,Ashlee E Williams,Alexander W Alund,Ezazul Haque,Gopi S Gadupudi,Larry W RobertsonPubMed 31820026
- International journal of molecular medicine 40:1860-18682017Insulin-like growth factor binding protein-6 released from human mesenchymal stem cells confers neuronal protection through IGF-1R-mediated signaling.Applications:Unspecified applicationReactive species:Unspecified reactive speciesHyo-Jin Jeon et. al.PubMed 29039467
Key facts
- Detection method
- Colorimetric
- Sample types
- Cell Lysate, Cell culture supernatant, EDTA Plasma, Heparin Plasma, Serum
- Assay type
- Sandwich (quantitative)
- Reactive species
- Rat
- Range
- 62.5 - 4000 pg/mL
- Assay time
- 3h 30m
- Sensitivity
- < 5 pg/mL
Reactivity data
| Application | Reactivity | Dilution info | Notes |
|---|---|---|---|
Application sELISA | Reactivity Reacts | Dilution info - | Notes - |
Target data
Function
The insulin-like growth factors, isolated from plasma, are structurally and functionally related to insulin but have a much higher growth-promoting activity. May be a physiological regulator of [1-14C]-2-deoxy-D-glucose (2DG) transport and glycogen synthesis in osteoblasts. Stimulates glucose transport in bone-derived osteoblastic (PyMS) cells and is effective at much lower concentrations than insulin, not only regarding glycogen and DNA synthesis but also with regard to enhancing glucose uptake. May play a role in synapse maturation. Ca(2+)-dependent exocytosis of IGF1 is required for sensory perception of smell in the olfactory bulb. Acts as a ligand for IGF1R. Binds to the alpha subunit of IGF1R, leading to the activation of the intrinsic tyrosine kinase activity which autophosphorylates tyrosine residues in the beta subunit thus initiating a cascade of down-stream signaling events leading to activation of the PI3K-AKT/PKB and the Ras-MAPK pathways. Binds to integrins ITGAV:ITGB3 and ITGA6:ITGB4. Its binding to integrins and subsequent ternary complex formation with integrins and IGFR1 are essential for IGF1 signaling. Induces the phosphorylation and activation of IGFR1, MAPK3/ERK1, MAPK1/ERK2 and AKT1 (By similarity). As part of the MAPK/ERK signaling pathway, acts as a negative regulator of apoptosis in cardiomyocytes via promotion of STUB1/CHIP-mediated ubiquitination and degradation of ICER-type isoforms of CREM (PubMed:20724525).
Alternative names
Igf-1, Igf1, Insulin-like growth factor 1, Insulin-like growth factor I, Somatomedin, IGF-I
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Rat IGF-1 ELISA Kit is a Sandwich (quantitative) ELISA kit for the measurement of Rat IGF-1 in Rat in Cell Lysate, Cell culture supernatant, EDTA Plasma, Heparin Plasma, Serum samples.
Key facts
- Detection method
- Colorimetric
- Sample types
- Cell Lysate, Cell culture supernatant, EDTA Plasma, Heparin Plasma, Serum
- Assay type
- Sandwich (quantitative)
- Reactive species
- Rat
- Range
- 62.5 - 4000 pg/mL
- Assay time
- 3h 30m
- Assay Platform
- Pre-coated microplate (12 x 8 well strips)
- Sensitivity
- < 5 pg/mL
Precision
Intra assay
| Sample | n | mean | SD | C.V. |
|---|---|---|---|---|
Sample Sample 1 | n 16 | mean 185 pg/mL | SD 9.99 | C.V. = 5.4 |
Sample Sample 2 | n 16 | mean 754 pg/mL | SD 56.55 | C.V. = 7.5 |
Sample Sample 3 | n 16 | mean 1874 pg/mL | SD 80.58 | C.V. = 4.3 |
Inter assay
| Sample | n | mean | SD | C.V. |
|---|---|---|---|---|
Sample Sample 2 | n 24 | mean 764 pg/mL | SD 69.52 | C.V. = 9.1 |
Sample Sample 1 | n 24 | mean 186 pg/mL | SD 11.16 | C.V. = 6 |
Sample Sample 3 | n 24 | mean 1861 pg/mL | SD 94.91 | C.V. = 5.1 |
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- -20°C
- Appropriate long-term storage conditions
- -20°C
- Storage information
- -20°C
Notes
The Rat IGF-1 Enzyme-Linked Immunosorbent Assay (ELISA) kit (ab213902) is designed for the quantitative measurement of Rat IGF-1 in cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA).
The ELISA kit is based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from mouse specific for IGF-1 has been pre-coated onto 96-well plates. Standards and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for IGF-1 is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex is added and unbound conjugates are washed away with PBS or TBS buffer. HRP substrate TMB is used to visualize HRP enzymatic reaction. TMB is catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the Rat IGF-1 amount of sample captured in plate.
Insulin-like growth factor 1 (IGF-1) that was once called somatomedin C, is a polypeptide protein hormone similar in molecular structure to insulin. It plays an important role in childhood growth and continues to have anabolic effects in adults. Human IGF1 is a single chain 70-amino acid polypeptide cross-linked by 3 disulfide bridges, with a calculated molecular mass of 7.6 kD. The IGF1 gene, mapped on 12q22-q24.1, contains 5 exons. Exons 1-4 encode the 195-amino acid precursor (IGF1B), and exons 1, 2, 3, and 5 encode the 153-residue peptide (IGF1A). The structure of IGF1 resembles that of IGF2. And the IGF1 and IGF2 genes have complex structures with multiple promoters. The expression of both genes is regulated at the levels of transcription, RNA processing, and translation. IGF-1 is produced primarily by the liver as an endocrine hormone as well as in target tissues in a paracrine/autocrine fashion. Moreover, approximately 98% of IGF-1 is always bound to one of 6 binding proteins (IGF-BP). Furthermore, IGF-1 is one of the most potent natural activators of the AKT signaling pathway, a stimulator of cell growth and multiplication and a potent inhibitor of programmed cell death.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
The insulin-like growth factor 1 or IGF1 is a protein hormone that plays an important mechanical role in growth and development. Alternative names for IGF1 include somatomedin C. This protein has a mass of approximately 7.6 kDa and is mainly produced by the liver. IGF1 is expressed in a range of tissues including muscle bone and cartilage reflecting its importance in various physiological processes. Its production is stimulated by growth hormone and it acts in an autocrine and paracrine manner to exert its effects.
- Biological function summary
IGF1 stimulates growth and proliferation of cells most notably impacting the development of the skeletal system and the regulation of apoptosis. It is not part of a larger protein complex but it interacts closely with IGF1 receptor to execute its functions. By binding to this receptor IGF1 activates intracellular signaling cascades that promote anabolic effects which include increased uptake of glucose and amino acids contributing to muscle and tissue growth.
- Pathways
IGF1 operates within the PI3K-Akt and MAPK signaling pathways which are significant for cell survival and proliferation. Its interaction with proteins like IGF binding protein 3 (IGFBP3) modulates its availability and activity in the bloodstream. These pathways are critical for mediating the effects of growth hormone highlighting the role of IGF1 in systemic growth and metabolism regulation.
- Associated diseases and disorders
Alterations in IGF1 levels associate with growth abnormalities such as Laron syndrome and acromegaly. Laron syndrome results from the body's inability to use IGF1 properly due to receptor defects while acromegaly occurs from excessive IGF1 production often due to pituitary tumors. Furthermore aberrant IGF1 signaling connects to cancer development where it can drive tumor growth and metastasis. Drugs targeting IGF1 and its pathway components continue to be researched for therapeutic potential in these conditions.
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Sandwich ELISA - Rat IGF-1 ELISA Kit (ab213902)
Rat IGF-1 ELISA Kit (ab213902) Standard Curve.
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