AB273153
Rat IgG ELISA kit - 30 minutes
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Rat IgG ELISA kit - 30 minutes is a ELISA for the measurement of Rat IgG - 30 minutes in in Cell Culture Media, Biofluids samples.
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- ELISA
Supplier Data
ELISA - Rat IgG ELISA kit - 30 minutes (AB273153)
Rat Immunoglobulin G Quantification Kit standard curve.
Example standard curve.
Complementary Products
Reactivity data
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Product details
The Rat IgG ELISA Kit (ab273153) provides a rapid and easy method (one step ELISA) for the quantitative determination of rat IgG in cell culture supernatant and rat ascitic fluid.
A polyclonal antibody specific to rat IgG (H+L) is pre-coated onto microwells. Samples and standards are pipetted into microwells and rat IgG present in the sample are bound by the capture antibody. Then, a HRP (horseradish peroxidase) conjugated anti-rat IgG (H+L) antibody is pipetted and incubated simultaneously with samples. After washing microwells in order to remove any non specific binding, the ready to use substrate solution (TMB) is added to microwells and color develops proportionally to the amount of rat IgG in the sample. Color development is then stopped by addition of stop solution. Absorbance is measured at 450 nm.
Precision
[ { "reproducibilityType": "Inter", "sample": "Sample D 1/100", "replicates": 30, "mean": null, "standardDeviation": "3.2", "coefficientOfVariability": null }, { "reproducibilityType": "Inter", "sample": "Sample D 1/400", "replicates": 30, "mean": null, "standardDeviation": "3.59", "coefficientOfVariability": null }, { "reproducibilityType": "Inter", "sample": "Sample D 1/6400", "replicates": 30, "mean": null, "standardDeviation": "3.13", "coefficientOfVariability": null }, { "reproducibilityType": "Intra", "sample": "Sample A 1/10", "replicates": 10, "mean": "5.18 µg/mL", "standardDeviation": "2.94", "coefficientOfVariability": null }, { "reproducibilityType": "Intra", "sample": "Sample B 1/10", "replicates": 10, "mean": "2.39 µg/mL", "standardDeviation": "3.48", "coefficientOfVariability": null }, { "reproducibilityType": "Intra", "sample": "Sample C 1/10", "replicates": 10, "mean": "1.34 µg/mL", "standardDeviation": "4.61", "coefficientOfVariability": null } ]
What's included?
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Properties and storage information
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
+4°C
Product protocols
- Visit the General protocols
- Visit the Troubleshooting
- Download websiteProtocolBooklet|en
Target data
Product promise
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