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AB189578

Rat IgG ELISA Kit

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(10 Publications)

Rat IgG ELISA Kit is a single-wash 90-min Simplestep used to quantify Rat IgG with a sensitivity of 55 pg/mL. The assay uses a simple mix-wash-read protocol with just one incubation and one wash step.

- Colorimetric Sandwich ELISA - 450 nm readout : works on any standard plate reader
- Different formats for different needs: 10x96 plates for bulk orders and 384-well plate for higher throughput
- Cited in over 10 citations

View Alternative Names

Immunoglobulin heavy constant gamma 1, Ig gamma-1 chain C region, Ig gamma-1 chain C region EU, Ig gamma-1 chain C region KOL, Ig gamma-1 chain C region NIE, IGHG1

6 Images
ELISA - Rat IgG ELISA Kit (AB189578)
  • ELISA

Supplier Data

ELISA - Rat IgG ELISA Kit (AB189578)
Sandwich ELISA - Rat IgG ELISA Kit (AB189578)
  • sELISA

Lab

Sandwich ELISA - Rat IgG ELISA Kit (AB189578)

Interpolated concentration of native IgG was measured in duplicate at different sample concentrations in 96-well vs. 384-well plates. Undiluted samples are 1 : 4,000,000 rat serum. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). Sample dilutions are made in Sample Diluent NS.

Sandwich ELISA - Rat IgG ELISA Kit (AB189578)
  • sELISA

Lab

Sandwich ELISA - Rat IgG ELISA Kit (AB189578)

Example of rat IgG standard curve in 96-well vs. 384-well plate. Background-subtracted data values (mean +/- SD) are graphed.

Sandwich ELISA - Rat IgG ELISA Kit (AB189578)
  • sELISA

Supplier Data

Sandwich ELISA - Rat IgG ELISA Kit (AB189578)

Example of rat IgG standard curve. The rat IgG standard curve was prepared as described. Raw data values are shown in the table. Background-subtracted data values (mean +/- SD) are graphed.

Sandwich ELISA - Rat IgG ELISA Kit (AB189578)
  • sELISA

Supplier Data

Sandwich ELISA - Rat IgG ELISA Kit (AB189578)

Example of rat IgG standard curve in Sample Diluent NS.

Background-subtracted data values (mean +/- SD) are graphed.

Sandwich ELISA - Rat IgG ELISA Kit (AB189578)
  • sELISA

Supplier Data

Sandwich ELISA - Rat IgG ELISA Kit (AB189578)

Native rat IgG was measured in the following biological samples in a 2‑fold dilution series. Sample dilutions are made in Sample Diluent NS.

Linearity of dilution is determined based on interpolated values from the standard curve. Linearity of dilution defines a sample concentration interval in which interpolated target concentrations are directly proportional to sample dilution.

Key facts

Detection method

Colorimetric

Sample types

Cell culture supernatant, Citrate plasma, EDTA Plasma, Heparin Plasma, Serum

Reacts with

Rat

Assay type

Sandwich (quantitative)

Sensitivity

= 55 pg/mL

Range

0.31 - 20 ng/mL

Assay time

1h 30m

Assay Platform

Microplate

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Product details

Rat IgG ELISA Kit ab189578 is a rapid single-wash 90-min Sandwich ELISA to measure Rat IgG in cell culture supernatant, citrate plasma, EDTA plasma, heparin plasma, serum. This SimpleStep sensitivity is 55 pg/mL.

How the assay works

Rat IgG SimpleStep ELISA®employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details.

Assay Specificity

Our SimpleStep ELISA® kits use recombinant monoclonal antibodies rigorously validated to ensure the highest level of consistency and reproducibility, improved sensitivity and specificity and ease of scalability and security of supply.
Please refer to our protocol booklet for more details.

Rat IgG ELISA Kit ab189578 protocol summary

1. Mix: add samples/standards to the wells together with the capture and detector antibody cocktail. Incubate 1 hr at room temperature
2. Wash
3. Add TMB development solution - incubate for 10 min
4. Add Stop solution
5. Read the results on a plate reader at 450 nm

Save your precious samples

Our 384-well Rat IgG ELISA Kit only requires a maximum of 12.5µL sample

There are four classes of immunoglobulins in rat: IgA, IgE, IgM, and IgG. IgG is the most abundant immunoglobulin and is equally distributed in blood and tissue. In rat, the IgG class is further divided into four subclasses: IgG1, IgG2a, IgG2b, and IgG2c. The general immunoglobulin structure is composed of four polypeptide chains, two heavy and two light chains linked together and to each other by disulfide bonds, creating a tetrameric quaternary structure. The resulting tetramer creates two identical halves which together form a Y like structure. While the amino-terminal portions that exhibits highly variable amino-acid composition are involved in antigen binding, the C terminal constant parts are involved in complement binding, placental passage and binding to cell membrane.

IgG is involved in response to a foreign antigen and the presence of IgG usually signifies a mature antibody response. IgG has a molecular weight of about 150 kDa, can bind to many pathogens and also plays an important role in antibody dependent cell-mediated cytotoxicity. Typically rat serum and plasma samples contain about 7 to 10 mg/ml of IgG.

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Precision

[ { "reproducibilityType": "Inter", "sample": "Overall", "replicates": 3, "mean": null, "standardDeviation": null, "coefficientOfVariability": "3.61" }, { "reproducibilityType": "Intra", "sample": "Overall", "replicates": 3, "mean": null, "standardDeviation": null, "coefficientOfVariability": "2.66" } ]
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Recovery

[ { "sample": "10F DMEM Medium", "range": "67.5 - 98 %", "average": "= 79.1" }, { "sample": "Antibody Diluent 4B", "range": "99.4 - 110.97 %", "average": "= 103.7" }, { "sample": "OF DMEM Medium", "range": "62.8 - 94 %", "average": "= 76.5" } ]
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What's included?

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Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
+4°C
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Constant region of immunoglobulin heavy chains. Immunoglobulins, also known as antibodies, are membrane-bound or secreted glycoproteins produced by B lymphocytes. In the recognition phase of humoral immunity, the membrane-bound immunoglobulins serve as receptors which, upon binding of a specific antigen, trigger the clonal expansion and differentiation of B lymphocytes into immunoglobulins-secreting plasma cells. Secreted immunoglobulins mediate the effector phase of humoral immunity, which results in the elimination of bound antigens (PubMed : 20176268, PubMed : 22158414). The antigen binding site is formed by the variable domain of one heavy chain, together with that of its associated light chain. Thus, each immunoglobulin has two antigen binding sites with remarkable affinity for a particular antigen. The variable domains are assembled by a process called V-(D)-J rearrangement and can then be subjected to somatic hypermutations which, after exposure to antigen and selection, allow affinity maturation for a particular antigen (PubMed : 17576170, PubMed : 20176268). Mediates IgG effector functions on monocytes triggering ADCC of virus-infected cells.
See full target information IGHG1
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Publications (10)

Recent publications for all applications. Explore the full list and refine your search

Pharmaceutical research 40:1223-1238 PubMed36949370

2023

Mechanisms of Obesity-Induced Changes in Pharmacokinetics of IgG in Rats.

Applications

Unspecified application

Species

Unspecified reactive species

Xizhe Gao,Yi-Hua Sheng,Sijia Yu,Jiadong Li,Raymond Rosa,Simone Girgis,Tiffany Guo,Luigi Brunetti,Leonid Kagan

Pharmaceutics 15: PubMed36839832

2023

Relationship between Body Composition and Serum Immunoglobulin Concentrations after Administration of Intravenous Immune Globulin-Preclinical and Clinical Evidence.

Applications

Unspecified application

Species

Unspecified reactive species

Luigi Brunetti,Helene Chapy,Ronald G Nahass,Rebecca Moore,Andrew Wassef,Derek Adler,Edward Yurkow,Leonid Kagan

Saudi journal of biological sciences 29:103313 PubMed35707823

2022

Melatonin relieves diabetic complications and regenerates pancreatic beta cells by the reduction in NF-kB expression in streptozotocin induced diabetic rats.

Applications

Unspecified application

Species

Unspecified reactive species

Alyaa Farid,Passant Moussa,Manar Youssef,Merna Haytham,Ali Shamy,Gehan Safwat

Molecular medicine (Cambridge, Mass.) 27:97 PubMed34479474

2021

The antioxidant N-acetylcysteine promotes immune response and inhibits epithelial-mesenchymal transition to alleviate pulmonary fibrosis in chronic obstructive pulmonary disease by suppressing the VWF/p38 MAPK axis.

Applications

Unspecified application

Species

Unspecified reactive species

Lanlan Zhu,Fei Xu,Xiuhua Kang,Jing Zhou,Qinqin Yao,Yang Lin,Wei Zhang

Scientific reports 9:20357 PubMed31889151

2019

Complement activation by autoantigen recognition in the growth process of benign prostatic hyperplasia.

Applications

Unspecified application

Species

Unspecified reactive species

Junya Hata,Takeshi Machida,Kanako Matsuoka,Seiji Hoshi,Hidenori Akaihata,Hiroyuki Hiraki,Toshiyuki Suzuki,Soichiro Ogawa,Masao Kataoka,Nobuhiro Haga,Kei Ishibashi,Yoshimi Homma,Hideharu Sekine,Yoshiyuki Kojima

Advanced materials (Deerfield Beach, Fla.) 31:e1806957 PubMed30856290

2019

A Dual-Bioresponsive Drug-Delivery Depot for Combination of Epigenetic Modulation and Immune Checkpoint Blockade.

Applications

Unspecified application

Species

Unspecified reactive species

Huitong Ruan,Quanyin Hu,Di Wen,Qian Chen,Guojun Chen,Yifei Lu,Jinqiang Wang,Hao Cheng,Weiyue Lu,Zhen Gu

Investigative ophthalmology & visual science 60:255-264 PubMed30649152

2019

The Protective Effect of a Topical Mucin Secretagogue on Ocular Surface Damage Induced by Airborne Carbon Black Exposure.

Applications

Unspecified application

Species

Unspecified reactive species

Xiangzhe Li,Boram Kang,Youngsub Eom,Hyung Keun Lee,Hyo Myung Kim,Jong Suk Song

Journal of pharmaceutical sciences 107:1987-1994 PubMed29551353

2018

Evaluation of the Effects of Animal Growth and Previous Exposure on the Pharmacokinetics of Rituximab in Rats.

Applications

sELISA

Species

Rat

Hélène Chapy,Leonid Kagan

Investigative ophthalmology & visual science 57:6580-6590 PubMed27918832

2016

The Effect of Ambient Titanium Dioxide Microparticle Exposure to the Ocular Surface on the Expression of Inflammatory Cytokines in the Eye and Cervical Lymph Nodes.

Applications

Unspecified application

Species

Unspecified reactive species

Youngsub Eom,Jong Suk Song,Hyun Kyu Lee,Boram Kang,Hyeon Chang Kim,Hyung Keun Lee,Hyo Myung Kim

Molecular immunology 78:38-47 PubMed27588825

2016

Role of IgM and angiotensin II Type I receptor autoantibodies in local complement activation in placental ischemia-induced hypertension in the rat.

Applications

Unspecified application

Species

Unspecified reactive species

Jean F Regal,Megan E Strehlke,Jenna M Peterson,Cameron R Wing,Jordan E Parker,Noel Fernando Nieto,Lynne T Bemis,Jeffrey S Gilbert,Sherry D Fleming
View all publications
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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions
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