Rat IgG ELISA Kit is a single-wash 90-min Simplestep used to quantify Rat IgG with a sensitivity of 55 pg/mL. The assay uses a simple mix-wash-read protocol with just one incubation and one wash step.
- Colorimetric Sandwich ELISA - 450 nm readout : works on any standard plate reader
- Different formats for different needs: 10x96 plates for bulk orders and 384-well plate for higher throughput
- Cited in over 10 citations
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- Pharmaceutical research 40:1223-12382023Mechanisms of Obesity-Induced Changes in Pharmacokinetics of IgG in Rats.Applications:Unspecified applicationReactive species:Unspecified reactive speciesXizhe Gao,Yi-Hua Sheng,Sijia Yu,Jiadong Li,Raymond Rosa,Simone Girgis,Tiffany Guo,Luigi Brunetti,Leonid KaganPubMed 36949370
- Pharmaceutics 15:2023Relationship between Body Composition and Serum Immunoglobulin Concentrations after Administration of Intravenous Immune Globulin-Preclinical and Clinical Evidence.Applications:Unspecified applicationReactive species:Unspecified reactive speciesLuigi Brunetti,Helene Chapy,Ronald G Nahass,Rebecca Moore,Andrew Wassef,Derek Adler,Edward Yurkow,Leonid KaganPubMed 36839832
- Saudi journal of biological sciences 29:1033132022Melatonin relieves diabetic complications and regenerates pancreatic beta cells by the reduction in NF-kB expression in streptozotocin induced diabetic rats.Applications:Unspecified applicationReactive species:Unspecified reactive speciesAlyaa Farid,Passant Moussa,Manar Youssef,Merna Haytham,Ali Shamy,Gehan SafwatPubMed 35707823
- Molecular medicine (Cambridge, Mass.) 27:972021The antioxidant N-acetylcysteine promotes immune response and inhibits epithelial-mesenchymal transition to alleviate pulmonary fibrosis in chronic obstructive pulmonary disease by suppressing the VWF/p38 MAPK axis.Applications:Unspecified applicationReactive species:Unspecified reactive speciesLanlan Zhu,Fei Xu,Xiuhua Kang,Jing Zhou,Qinqin Yao,Yang Lin,Wei ZhangPubMed 34479474
- Scientific reports 9:203572019Complement activation by autoantigen recognition in the growth process of benign prostatic hyperplasia.Applications:Unspecified applicationReactive species:Unspecified reactive speciesJunya Hata et. al.PubMed 31889151
- Advanced materials (Deerfield Beach, Fla.) 31:e18069572019A Dual-Bioresponsive Drug-Delivery Depot for Combination of Epigenetic Modulation and Immune Checkpoint Blockade.Applications:Unspecified applicationReactive species:Unspecified reactive speciesHuitong Ruan,Quanyin Hu,Di Wen,Qian Chen,Guojun Chen,Yifei Lu,Jinqiang Wang,Hao Cheng,Weiyue Lu,Zhen GuPubMed 30856290
- Investigative ophthalmology & visual science 60:255-2642019The Protective Effect of a Topical Mucin Secretagogue on Ocular Surface Damage Induced by Airborne Carbon Black Exposure.Applications:Unspecified applicationReactive species:Unspecified reactive speciesXiangzhe Li,Boram Kang,Youngsub Eom,Hyung Keun Lee,Hyo Myung Kim,Jong Suk SongPubMed 30649152
- Journal of pharmaceutical sciences 107:1987-19942018Evaluation of the Effects of Animal Growth and Previous Exposure on the Pharmacokinetics of Rituximab in Rats.
- Investigative ophthalmology & visual science 57:6580-65902016The Effect of Ambient Titanium Dioxide Microparticle Exposure to the Ocular Surface on the Expression of Inflammatory Cytokines in the Eye and Cervical Lymph Nodes.Applications:Unspecified applicationReactive species:Unspecified reactive speciesYoungsub Eom,Jong Suk Song,Hyun Kyu Lee,Boram Kang,Hyeon Chang Kim,Hyung Keun Lee,Hyo Myung KimPubMed 27918832
- Molecular immunology 78:38-472016Role of IgM and angiotensin II Type I receptor autoantibodies in local complement activation in placental ischemia-induced hypertension in the rat.Applications:Unspecified applicationReactive species:Unspecified reactive speciesJean F Regal et. al.PubMed 27588825
Key facts
- Detection method
- Colorimetric
- Sample types
- Cell culture supernatant, Citrate plasma, EDTA Plasma, Heparin Plasma, Serum
- Assay type
- Sandwich (quantitative)
- Reactive species
- Rat
- Range
- 0.31 - 20 ng/mL
- Assay time
- 1h 30m
- Sensitivity
- = 55 pg/mL
Reactivity data
| Application | Reactivity | Dilution info | Notes |
|---|---|---|---|
Application sELISA | Reactivity Reacts | Dilution info - | Notes - |
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Target data
Function
Constant region of immunoglobulin heavy chains. Immunoglobulins, also known as antibodies, are membrane-bound or secreted glycoproteins produced by B lymphocytes. In the recognition phase of humoral immunity, the membrane-bound immunoglobulins serve as receptors which, upon binding of a specific antigen, trigger the clonal expansion and differentiation of B lymphocytes into immunoglobulins-secreting plasma cells. Secreted immunoglobulins mediate the effector phase of humoral immunity, which results in the elimination of bound antigens (PubMed:20176268, PubMed:22158414). The antigen binding site is formed by the variable domain of one heavy chain, together with that of its associated light chain. Thus, each immunoglobulin has two antigen binding sites with remarkable affinity for a particular antigen. The variable domains are assembled by a process called V-(D)-J rearrangement and can then be subjected to somatic hypermutations which, after exposure to antigen and selection, allow affinity maturation for a particular antigen (PubMed:17576170, PubMed:20176268). Mediates IgG effector functions on monocytes triggering ADCC of virus-infected cells.
Alternative names
Immunoglobulin heavy constant gamma 1, Ig gamma-1 chain C region, Ig gamma-1 chain C region EU, Ig gamma-1 chain C region KOL, Ig gamma-1 chain C region NIE, IGHG1
What's included?
Recommended products
Rat IgG ELISA Kit is a single-wash 90-min Simplestep used to quantify Rat IgG with a sensitivity of 55 pg/mL. The assay uses a simple mix-wash-read protocol with just one incubation and one wash step.
- Colorimetric Sandwich ELISA - 450 nm readout : works on any standard plate reader
- Different formats for different needs: 10x96 plates for bulk orders and 384-well plate for higher throughput
- Cited in over 10 citations
Key facts
- Detection method
- Colorimetric
- Sample types
- Cell culture supernatant, Citrate plasma, EDTA Plasma, Heparin Plasma, Serum
- Assay type
- Sandwich (quantitative)
- Reactive species
- Rat
- Range
- 0.31 - 20 ng/mL
- Assay time
- 1h 30m
- Assay Platform
- Microplate
- Sensitivity
- = 55 pg/mL
Precision
Intra assay
| Sample | n | C.V. |
|---|---|---|
Sample Overall | n 3 | C.V. 2.66 |
Inter assay
| Sample | n | C.V. |
|---|---|---|
Sample Overall | n 3 | C.V. 3.61 |
Recovery
Sample specific recovery
| Sample type | Average % | Range |
|---|---|---|
Sample type 10F DMEM Medium | Average % = 79.1 | Range 67.5 - 98 % |
Sample type Antibody Diluent 4B | Average % = 103.7 | Range 99.4 - 110.97 % |
Sample type OF DMEM Medium | Average % = 76.5 | Range 62.8 - 94 % |
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- +4°C
- Storage information
- +4°C
Notes
Rat IgG ELISA Kit (ab189578) is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of IgG protein in cell culture supernatant, cit plasma, edta plasma, hep plasma, and serum. It uses our proprietary SimpleStep ELISA® technology. Quantitate Rat IgG with 55 pg/ml sensitivity.
SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:
- Single-wash protocol reduces assay time to 90 minutes or less
- High sensitivity, specificity and reproducibility from superior antibodies
- Fully validated in biological samples
- 96-wells plate breakable into 12 x 8 wells strips
A 384-well SimpleStep ELISA® microplate (Pre-coated 384 well Microplate SimpleStep ELISA® ab203359) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.
ASSAY SPECIFICITY
This kit recognizes native rat IgG in serum, plasma and cell culture supernatant samples.
SPECIES REACTIVITY
This kit recognizes rat IgG protein and cross reacts with hamster IgG.
Other species reactivity was determined by measuring 1 to 1 million diluted serum samples of various species, interpolating the protein concentrations from the rat IgG standard curve, and expressing the interpolated concentrations as a percentage of the protein concentration in rat serum assayed at the same dilution.
Reactivity < 0.3% was determined for the following species: Mouse, Guinea Pig, Rabbit, Dog, Goat, Pig, Cow, Human, Chicken
Hamster serum displays 71.4% cross-reactvity.
There are four classes of immunoglobulins in rat: IgA, IgE, IgM, and IgG. IgG is the most abundant immunoglobulin and is equally distributed in blood and tissue. In rat, the IgG class is further divided into four subclasses: IgG1, IgG2a, IgG2b, and IgG2c. The general immunoglobulin structure is composed of four polypeptide chains, two heavy and two light chains linked together and to each other by disulfide bonds, creating a tetrameric quaternary structure. The resulting tetramer creates two identical halves which together form a Y like structure. While the amino-terminal portions that exhibits highly variable amino-acid composition are involved in antigen binding, the C terminal constant parts are involved in complement binding, placental passage and binding to cell membrane.
IgG is involved in response to a foreign antigen and the presence of IgG usually signifies a mature antibody response. IgG has a molecular weight of about 150 kDa, can bind to many pathogens and also plays an important role in antibody dependent cell-mediated cytotoxicity. Typically rat serum and plasma samples contain about 7 to 10 mg/ml of IgG.
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5 product images
Sandwich ELISA - Rat IgG ELISA Kit (ab189578)
Example of rat IgG standard curve in Sample Diluent NS.
Background-subtracted data values (mean +/- SD) are graphed.
Sandwich ELISA - Rat IgG ELISA Kit (ab189578)
Example of rat IgG standard curve. The rat IgG standard curve was prepared as described. Raw data values are shown in the table. Background-subtracted data values (mean +/- SD) are graphed.
Sandwich ELISA - Rat IgG ELISA Kit (ab189578)
Native rat IgG was measured in the following biological samples in a 2‑fold dilution series. Sample dilutions are made in Sample Diluent NS.
Linearity of dilution is determined based on interpolated values from the standard curve. Linearity of dilution defines a sample concentration interval in which interpolated target concentrations are directly proportional to sample dilution.
Sandwich ELISA - Rat IgG ELISA Kit (ab189578)
Interpolated concentration of native IgG was measured in duplicate at different sample concentrations in 96-well vs. 384-well plates. Undiluted samples are 1:4,000,000 rat serum. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). Sample dilutions are made in Sample Diluent NS.
Sandwich ELISA - Rat IgG ELISA Kit (ab189578)
Example of rat IgG standard curve in 96-well vs. 384-well plate. Background-subtracted data values (mean +/- SD) are graphed.
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