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AB157738

Rat IgM ELISA Kit

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(5 Publications)

Rat IgM ELISA Kit is a sandwich ELISA designed to quantify Rat IgM with a sensitivity of 3.1324 ng/mL.

- Colorimetric sandwich ELISA - 450 nm readout - works on any plate reader
- Wide dynamic range - quantifies 31.25 - 1000 ng/mL
1 Images
Sandwich ELISA - Rat IgM ELISA Kit (AB157738)
  • sELISA

Supplier Data

Sandwich ELISA - Rat IgM ELISA Kit (AB157738)

Representative standard curve using ab157738 IgM Rat ELISA Kit.

Key facts

Detection method

Colorimetric

Sample types

Plasma, Serum

Reacts with

Rat

Assay type

Sandwich

Results type

Quantitative

Sensitivity

= 3.1324 ng/mL

Range

31.25 - 1000 ng/mL

Assay Platform

Microplate

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Product details

Rat IgM ELISA Kit ab157738 is a sandwich ELISA to measure Rat IgM in serum, plasma with a sensitivity of 3.1324 ng/ml.

How the assay works

In this assay the IgM present in samples reacts with the anti-IgM antibodies which have been adsorbed to the surface of polystyrene microtiter wells. After the removal of unbound proteins by washing, anti-IgM antibodies conjugated with horseradish peroxidase (HRP) are added. These enzyme-labeled antibodies form complexes with the previously bound IgM. Following another washing step, the enzyme bound to the immunosorbent is assayed by the addition of a chromogenic substrate, 3,3',5,5'-tetramethylbenzidine (TMB). The quantity of bound enzyme varies directly with the concentration of IgM in the sample tested; thus, the absorbance, at 450 nm, is a measure of the concentration of IgM in the test sample. The quantity of IgM in the test sample can be interpolated from the standard curve constructed from the standards, and corrected for sample dilution.

Assay Specificity

Our ELISA kits are rigorously validated to ensure the highest level of consistency and reproducibility. Please check the protocol booklet for more details

Rat IgM ELISA Kit ab157738 protocol summary

1. Add standard or sample to each well used. Incubate at room temperature.
2. Aspirate and wash each well.
3. Add prepared HRP labeled secondary detector antibody. Incubate at room temperature
4. Aspirate and wash each well.
5. Add Chromogen Substrate Solution to each well.
6. Immediately begin recording the color development
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Precision

[ { "reproducibilityType": "Inter", "sample": "Overall", "replicates": 0, "mean": null, "standardDeviation": null, "coefficientOfVariability": null }, { "reproducibilityType": "Intra", "sample": "Overall", "replicates": 0, "mean": null, "standardDeviation": null, "coefficientOfVariability": "< 10" } ]
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Recovery

[ { "sample": "Serum", "range": null, "average": "> 85" } ]
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What's included?

{ "values": { "1x96Tests": { "sellingSize": "1 x 96 Tests", "publicAssetCode":"ab157738-1x96Tests", "assetComponentDetails": [ { "size":"1 x 1 Vial", "name":"Rat IgM Calibrator (Lyophilized)", "number":"AB157738-CMP01", "productcode":"" }, { "size":"1 x 1 Unit", "name":"Rat IgM ELISA Microplate", "number":"AB157738-CMP02", "productcode":"" }, { "size":"1 x 150 µL", "name":"100X HRP-conjugated anti-rat IgM antibody", "number":"AB157738-CMP05", "productcode":"" }, { "size":"1 x 12 mL", "name":"Chromogen Substrate Solution", "number":"AB157738-CMP04", "productcode":"" }, { "size":"1 x 12 mL", "name":"Stop Solution", "number":"AB157738-CMP03", "productcode":"" }, { "size":"1 x 50 mL", "name":"20X Wash Buffer Concentrate", "number":"AB157738-CMP06", "productcode":"" }, { "size":"1 x 50 mL", "name":"5X Diluent Concentrate", "number":"AB157738-CMP07", "productcode":"" } ] } } }
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Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
Please refer to protocols
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

IgM also known as immunoglobulin M is a type of antibody with a molecular mass of approximately 970 kDa. It is the largest antibody present in the human circulatory system mostly found in plasma and lymphatic tissue. IgM is secreted primarily by B cells as a pentamer with a J chain providing a high avidity for antigens. When bound to an antigen IgM can activate the complement system a part of the immune system that helps clear pathogens from an organism.
Biological function summary

IgM acts as the first line of defense during the initial stages of an immune response. It plays an important part in agglutination and neutralization of pathogens forming a complex that alerts other components of the immune system to take action. IgM exists before class-switching to IgG providing immediate protection. It also plays a role in antibody-dependent cellular cytotoxicity. Although not typically a long-term solution its presence is critical for providing initial protection until more specific antibodies like IgG and IgA are produced.

Pathways

The immune response that involves IgM begins with the activation of the classical complement pathway. This pathway bridges the innate and adaptive immunity ensuring the communication and coordination of immune responses. The pathway involves C1q protein binding to IgM-antigen complexes. IgM also interacts with antigen-presenting cells facilitating the processing of antigens and interaction with T cells. These interactions connect IgM with other proteins like IgG and IgA influencing various immune response pathways.

IgM is associated with conditions such as Waldenström's macroglobulinemia and rheumatoid arthritis. Waldenström's macroglobulinemia involves the overproduction of IgM by clonal B cells leading to symptoms like anemia bleeding and neuropathy. Rheumatoid arthritis shows increased IgM levels as part of the autoimmune response affecting joints. In these diseases the IgM antibody interacts with rheumatoid factor and other immune proteins involved in inflammation demonstrating its significant role in autoimmunity and abnormal immune responses.
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Product protocols

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Target data

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Publications (5)

Recent publications for all applications. Explore the full list and refine your search

BMC complementary medicine and therapies 25:21 PubMed39844243

2025

Physalis peruviana calyces extract ameliorate oxidative stress, inflammation, and immune loss in rats-exposed to hexaflumuron.

Applications

Unspecified application

Species

Unspecified reactive species

Mona M Hashem,Eman I Hassanen,Neven H Hassan,Marwa A Ibrahim,Marwa Y Issa,Mohamed A Farag,Sherif A Hamdy

Foods (Basel, Switzerland) 12: PubMed37835305

2023

Immune-Boosting Potentiating Properties of Hydroalcoholic Extract in Cyclophosphamide-Induced Immunosuppression in Rats.

Applications

Unspecified application

Species

Unspecified reactive species

Hassan Barakat,Raghad I Alkhurayji,Thamer Aljutaily

Molecular medicine (Cambridge, Mass.) 27:97 PubMed34479474

2021

The antioxidant N-acetylcysteine promotes immune response and inhibits epithelial-mesenchymal transition to alleviate pulmonary fibrosis in chronic obstructive pulmonary disease by suppressing the VWF/p38 MAPK axis.

Applications

Unspecified application

Species

Unspecified reactive species

Lanlan Zhu,Fei Xu,Xiuhua Kang,Jing Zhou,Qinqin Yao,Yang Lin,Wei Zhang

American journal of physiology. Heart and circulat 317:H732-H742 PubMed31397167

2019

Role of B1 and B2 lymphocytes in placental ischemia-induced hypertension.

Applications

Unspecified application

Species

Unspecified reactive species

Connor F Laule,Evan J Odean,Cameron R Wing,Kate M Root,Kendra J Towner,Cassandra M Hamm,Jeffrey S Gilbert,Sherry D Fleming,Jean F Regal

Molecular immunology 78:38-47 PubMed27588825

2016

Role of IgM and angiotensin II Type I receptor autoantibodies in local complement activation in placental ischemia-induced hypertension in the rat.

Applications

Unspecified application

Species

Unspecified reactive species

Jean F Regal,Megan E Strehlke,Jenna M Peterson,Cameron R Wing,Jordan E Parker,Noel Fernando Nieto,Lynne T Bemis,Jeffrey S Gilbert,Sherry D Fleming
View all publications
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