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AB113350

Rat IL-1 alpha ELISA Kit

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(3 Publications)

Rat IL-1 alpha ELISA Kit is a Sandwich (quantitative) ELISA for the measurement of Rat IL-1 alpha in Rat in Biofluids, Cell Culture Media samples.

View Alternative Names

Interleukin-1 alpha, IL-1 alpha, Il1a

1 Images
Sandwich ELISA - Rat IL-1 alpha ELISA Kit (AB113350)
  • sELISA

Unknown

Sandwich ELISA - Rat IL-1 alpha ELISA Kit (AB113350)

Representative standard curve using ab113350

Key facts

Detection method

Colorimetric

Sample types

Plasma, Cell culture supernatant, Serum

Reacts with

Rat

Assay type

Sandwich (quantitative)

Sensitivity

< 15 pg/mL

Range

11 - 8000 pg/mL

Assay Platform

Microplate

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Product details

Abcam's IL-1 alpha Rat ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of Rat IL-1 alpha in serum, plasma and cell culture supernatants.

This assay employs an antibody specific for IL-1 alpha coated on a 96-well plate. Standards and samples are pipetted into the wells and IL- 1 alpha present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-Rat IL-1 alpha antibody is added. After washing away unbound biotinylated antibody, HRP conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of IL-1 alpha bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.

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Recovery

[ { "sample": "Cell culture supernatant", "range": "76 - 95 %", "average": "= 87.48" }, { "sample": "Serum", "range": "69 - 88 %", "average": "= 79.95" }, { "sample": "Plasma", "range": "68 - 84 %", "average": "= 75.73" } ]
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What's included?

{ "values": { "1x96Tests": { "sellingSize": "1 x 96 Tests", "publicAssetCode":"ab113350-1x96Tests", "assetComponentDetails": [ { "size":"1 x 25 mL", "name":"20X Wash Buffer", "number":"AB113350-CMP03", "productcode":"" }, { "size":"1 x 8 mL", "name":"Stop Solution", "number":"AB113350-CMP07", "productcode":"" }, { "size":"1 x 15 mL", "name":"5X Assay Diluent B", "number":"AB113350-CMP05", "productcode":"" }, { "size":"1 x 30 mL", "name":"Assay Diluent C", "number":"AB113350-CMP06", "productcode":"" }, { "size":"2 x 1 Vial", "name":"Biotinylated anti-Rat IL-1 alpha", "number":"AB113350-CMP09", "productcode":"" }, { "size":"1 x 1 Unit", "name":"IL-1 alpha Microplate (12 x 8 wells)", "number":"AB113350-CMP01", "productcode":"" }, { "size":"1 x 200 µL", "name":"500X HRP-Streptavidin Concentrate", "number":"AB113350-CMP04", "productcode":"" }, { "size":"1 x 12 mL", "name":"TMB One-Step Substrate Reagent", "number":"AB113350-CMP08", "productcode":"" }, { "size":"2 x 1 Vial", "name":"Recombinant rat IL-1 alpha Standard (lyophilized)", "number":"AB113350-CMP02", "productcode":"" } ] } } }
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Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
-20°C
Appropriate long-term storage conditions
-20°C
Storage information
-20°C
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Product protocols

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Target data

Cytokine constitutively present intracellularly in nearly all resting non-hematopoietic cells that plays an important role in inflammation and bridges the innate and adaptive immune systems. After binding to its receptor IL1R1 together with its accessory protein IL1RAP, forms the high affinity interleukin-1 receptor complex. Signaling involves the recruitment of adapter molecules such as MYD88, IRAK1 or IRAK4. In turn, mediates the activation of NF-kappa-B and the three MAPK pathways p38, p42/p44 and JNK pathways. Within the cell, acts as an alarmin and cell death results in its liberation in the extracellular space after disruption of the cell membrane to induce inflammation and alert the host to injury or damage. In addition to its role as a danger signal, which occurs when the cytokine is passively released by cell necrosis, directly senses DNA damage and acts as signal for genotoxic stress without loss of cell integrity.
See full target information Il1a
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Publications (3)

Recent publications for all applications. Explore the full list and refine your search

Journal of cellular and molecular medicine : PubMed33067927

2020

MR-guided pulsed focused ultrasound improves mesenchymal stromal cell homing to the myocardium.

Applications

Unspecified application

Species

Unspecified reactive species

Kee W Jang,Tsang-Wei Tu,Robert B Rosenblatt,Scott R Burks,Joseph A Frank

Gut 70:379-387 PubMed32241903

2020

Differential inflammasome activation predisposes to acute-on-chronic liver failure in human and experimental cirrhosis with and without previous decompensation.

Applications

Unspecified application

Species

Unspecified reactive species

Sofia Monteiro,Josephine Grandt,Frank Erhard Uschner,Nina Kimer,Jan Lysgård Madsen,Robert Schierwagen,Sabine Klein,Christoph Welsch,Liliana Schäfer,Christian Jansen,Joan Claria,José Alcaraz-Quiles,Vicente Arroyo,Richard Moreau,Javier Fernandez,Flemming Bendtsen,Gautam Mehta,Lise Lotte Gluud,Søren Møller,Michael Praktiknjo,Jonel Trebicka

Radiation oncology (London, England) 10:195 PubMed26382926

2015

Risk of radiation-induced pneumonitis after helical and static-port tomotherapy in lung cancer patients and experimental rats.

Applications

Unspecified application

Species

Unspecified reactive species

Xianglan Zhang,You Keun Shin,Zhenlong Zheng,Lianhua Zhu,Ik Jae Lee
View all publications
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websiteProtocolBooklet
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