AB100767

Rat IL-1 beta ELISA Kit

Name: Rat IL-1 beta ELISA Kit (ab100767) | Abcam
Brand: Abcam Limited
SKU: AB100767
Price: 780 USD
Availability: InStock

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(29 Publications)

Rat IL-1 beta ELISA Kit is a Sandwich ELISA for the measurement of Rat IL-1 beta in Rat in Biofluids, Cell Culture Media samples.

View Alternative Names

Interleukin-1 beta, IL-1 beta, Il1b

2 Images

Sandwich ELISA - Rat IL-1 beta ELISA Kit (AB100767)

sELISA

Supplier Data

Sandwich ELISA - Rat IL-1 beta ELISA Kit (AB100767)

Representative standard curve using ab100767

sELISA

Unknown

Sandwich ELISA - Rat IL-1 beta ELISA Kit (AB100767)

Representative standard curve using ab100767

Key facts

Detection method

Colorimetric

Sample types

Plasma, Cell culture supernatant, Serum

Reacts with

Rat

Assay type

Sandwich

Results type

Quantitative

Sensitivity

< 80 pg/mL

Range

68.59 - 50000 pg/mL

Assay Platform

Microplate

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }

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Product details

Abcam's IL-1 beta Rat ELISA (Enzyme-Linked Immunosorbent Assay) Kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of rat IL-1 beta in serum, plasma and cell culture supernatants.

This assay employs an antibody specific for IL-1 beta coated on a 96-well plate. Standards and samples are pipetted into the wells and IL- 1 beta present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-rat IL-1 beta antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and colour develops in proportion to the amount of IL-1 beta bound. The Stop Solution changes the colour from blue to yellow, and the intensity of the colour is measured at 450 nm.

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Recovery

[ { "sample": "Cell culture supernatant", "range": "81 - 109 %", "average": "= 92.89" }, { "sample": "Serum", "range": "95 - 115 %", "average": "= 107.2" }, { "sample": "Plasma", "range": "89 - 108 %", "average": "= 97.55" } ]

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What's included?

{ "values": { "1x96Tests": { "sellingSize": "1 x 96 Tests", "publicAssetCode":"ab100767-1x96Tests", "assetComponentDetails": [ { "size":"1 x 25 mL", "name":"20X Wash Buffer", "number":"AB100767-CMP03", "productcode":"" }, { "size":"1 x 8 mL", "name":"Stop Solution", "number":"AB100767-CMP07", "productcode":"" }, { "size":"1 x 12 mL", "name":"TMB One-Step Substrate Reagent", "number":"AB100767-CMP08", "productcode":"" }, { "size":"1 x 30 mL", "name":"Assay Diluent A", "number":"AB100767-CMP05", "productcode":"" }, { "size":"2 x 1 Vial", "name":"Biotinylated anti-rat IL-1 beta", "number":"AB100767-CMP09", "productcode":"" }, { "size":"1 x 15 mL", "name":"5X Assay Diluent B", "number":"AB100767-CMP06", "productcode":"" }, { "size":"1 x 1 Unit", "name":"IL-1 beta Microplate (12 x 8 wells)", "number":"AB100767-CMP01", "productcode":"" }, { "size":"1 x 200 µL", "name":"200X HRP-Streptavidin Concentrate", "number":"AB100767-CMP04", "productcode":"" }, { "size":"2 x 1 Vial", "name":"Recombinant rat IL-1 beta Standard (lyophilized)", "number":"AB100767-CMP02", "productcode":"" } ] } } }

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Properties and storage information

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

-20°C

Appropriate long-term storage conditions

-20°C

Storage information

-20°C

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Product protocols

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Target data

Potent pro-inflammatory cytokine. Initially discovered as the major endogenous pyrogen, induces prostaglandin synthesis, neutrophil influx and activation, T-cell activation and cytokine production, B-cell activation and antibody production, and fibroblast proliferation and collagen production. Promotes Th17 differentiation of T-cells. Synergizes with IL12/interleukin-12 to induce IFNG synthesis from T-helper 1 (Th1) cells. Plays a role in angiogenesis by inducing VEGF production synergistically with TNF and IL6. Involved in transduction of inflammation downstream of pyroptosis : its mature form is specifically released in the extracellular milieu by passing through the gasdermin-D (GSDMD) pore.

See full target information Il1b

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Publications (29)

Recent publications for all applications. Explore the full list and refine your search

Biology 13: PubMed39596850

2024

In-Depth Analysis of L. Leaf Extract: Alleviating Pulmonary Histological Disturbances, Pro-Inflammatory Responses, and Oxidative Stress from Isolated or Combined Exposure to Inhaled Toluene and Noise in Rats.

Applications

Unspecified application

Species

Unspecified reactive species

Takoua Ben Attia,Sana Bahri,Sonia Ben Younes,Afef Nahdi,Ridha Ben Ali,Linda Bel Haj Kacem,Michèle Véronique El May,Eduardo Alberto López-Maldonado,Abada Mhamdi

Frontiers in pharmacology 15:1466824 PubMed39372212

2024

Evaluation of the beneficial effects of a GABA-based product containing on post-inflammatory irritable bowel syndrome: a preclinical study.

Applications

Unspecified application

Species

Unspecified reactive species

Elena Lucarini,Laura Benvenuti,Clelia Di Salvo,Vanessa D'Antongiovanni,Carolina Pellegrini,Giulia Valdiserra,Clara Ciampi,Luca Antonioli,Christian Lambiase,Lorenzo Cancelli,Antonio Grosso,Lorenzo Di Cesare Mannelli,Massimo Bellini,Carla Ghelardini,Matteo Fornai

Biology direct 18:23 PubMed37165439

2023

Inhibition of TRAF6 improves hyperlipidemic acute pancreatitis by alleviating pyroptosis in vitro and in vivo rat models.

Applications

Unspecified application

Species

Unspecified reactive species

Biwei Wei,Zhou Su,Huiying Yang,Yong Feng,Chunmei Meng,Zhihai Liang

Metabolites 13: PubMed36677055

2023

Quercetin, a Plant Flavonol Attenuates Diabetic Complications, Renal Tissue Damage, Renal Oxidative Stress and Inflammation in Streptozotocin-Induced Diabetic Rats.

Applications

Unspecified application

Species

Unspecified reactive species

Arshad Husain Rahmani,Mohammed A Alsahli,Amjad Ali Khan,Saleh A Almatroodi

Toxicology research 11:784-790 PubMed36337236

2022

Silica exposure activates non-canonical inflammasome complex in intratracheal instilled rat model.

Applications

Unspecified application

Species

Unspecified reactive species

Yingmei Niu,Shuangli Yang,Xiumei Hu

Clinical science (London, England : 1979) 136:1117-1137 PubMed35852150

2022

Maternal immune activation in rats induces dysfunction of placental leucine transport and alters fetal brain growth.

Applications

Unspecified application

Species

Unspecified reactive species

Hager M Kowash,Harry G Potter,Rebecca M Woods,Nick Ashton,Reinmar Hager,Joanna C Neill,Jocelyn D Glazier

Pharmaceutical biology 60:968-978 PubMed35588105

2022

Protective effects of lavender oil on sepsis-induced acute lung injury via regulation of the NF-κB pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Qian Xie,Yi Wang,Guo-Liang Zou

Frontiers in aging neuroscience 14:844534 PubMed35493929

2022

A Neuron, Microglia, and Astrocyte Triple Co-culture Model to Study Alzheimer's Disease.

Applications

Unspecified application

Species

Unspecified reactive species

Celia Luchena,Jone Zuazo-Ibarra,Jorge Valero,Carlos Matute,Elena Alberdi,Estibaliz Capetillo-Zarate

Biomedicines 10: PubMed35327330

2022

Fibrin, Bone Marrow Cells and Macrophages Interactively Modulate Cardiomyoblast Fate.

Applications

Unspecified application

Species

Unspecified reactive species

Inês Borrego,Aurélien Frobert,Guillaume Ajalbert,Jérémy Valentin,Cyrielle Kaltenrieder,Benoît Fellay,Michael Stumpe,Stéphane Cook,Joern Dengjel,Marie-Noëlle Giraud

Kidney & blood pressure research 47:256-269 PubMed35016182

2022

Ulinastatin Improves Renal Microcirculation by Protecting Endothelial Cells and Inhibiting Autophagy in a Septic Rat Model.

Applications

Unspecified application

Species

Unspecified reactive species

Tian Li,Xiaojun Ji,Jingfeng Liu,Xinjie Guo,Ran Pang,Haizhou Zhuang,Lei Dong,Meili Duan,Ang Li

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Rat IL-1 beta ELISA Kit

Sandwich ELISA - Rat IL-1 beta ELISA Kit (AB100767)

Sandwich ELISA - Rat IL-1 beta ELISA Kit (AB100767)

Key facts

Detection method

Sample types

Reacts with

Assay type

Results type

Sensitivity

Range

Assay Platform

Complementary Products

Assay Kits

Assay Kits

Secondary Antibodies

Assay Kits

Reactivity data

Product details

Recovery

What's included?

Properties and storage information

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Storage information

Product protocols

Target data

Publications (29)

Product promise