AB100768

Rat IL-1 beta ELISA Kit

Name: Rat IL-1 beta ELISA Kit (ab100768) | Abcam
Brand: Abcam Limited
SKU: AB100768
Rating: 2 (1 reviews)

(1 Review)

(125 Publications)

Rat IL-1 beta ELISA Kit is a Sandwich ELISA for the measurement of Rat IL-1 beta in Rat in Cell/Tissue Extracts samples.

View Alternative Names

Interleukin-1 beta, IL-1 beta, Il1b

4 Images

Sandwich ELISA - Rat IL-1 beta ELISA Kit (AB100768)

sELISA

Unknown

Sandwich ELISA - Rat IL-1 beta ELISA Kit (AB100768)

Rat IL-1b detected in cell lysates from NR8383.1 control cells (C) or cells stimulated for 6 hours with 5 ug x mL-1 of LPS (Sigma). Results shown for IL-1β levels in 10e6 cells, after background signal was subtracted (duplicates +/- SD).

sELISA

Unknown

Sandwich ELISA - Rat IL-1 beta ELISA Kit (AB100768)

Standard curve of Rt IL-1 beta, with background signal subtracted (duplicates; +/- SD).

sELISA

Supplier Data

Sandwich ELISA - Rat IL-1 beta ELISA Kit (AB100768)

Representative standard curve using ab100768

sELISA

Unknown

Sandwich ELISA - Rat IL-1 beta ELISA Kit (AB100768)

Rat IL-1b measured in biological fluids or in spleen lysate; background signal subtracted (duplicates +/- SD).

Key facts

Detection method

Colorimetric

Sample types

Cell culture extracts, Tissue Extracts

Reacts with

Rat

Assay type

Sandwich

Results type

Quantitative

Sensitivity

< 80 pg/mL

Range

68.59 - 50000 pg/mL

Assay Platform

Microplate

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }

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Product details

Abcam's IL-1 beta Rat ELISA (Enzyme-Linked Immunosorbent Assay) Kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of rat IL-1 beta in cell lysates and tissue lysates.

This assay employs an antibody specific for rat IL-1 beta coated on a 96-well plate. Standards and samples are pipetted into the wells and IL-1 beta present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-rat IL-1 beta antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and colour develops in proportion to the amount of IL-1 beta bound. The Stop Solution changes the colour from blue to yellow, and the intensity of the colour is measured at 450 nm.

REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.

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Precision

[ { "reproducibilityType": "Intra", "sample": "Overall", "replicates": 0, "mean": null, "standardDeviation": null, "coefficientOfVariability": "< 10" }, { "reproducibilityType": "Inter", "sample": "Overall", "replicates": 0, "mean": null, "standardDeviation": null, "coefficientOfVariability": "< 12" } ]

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Recovery

[ { "sample": "Cell culture extracts", "range": "88 - 105 %", "average": "= 96.22" }, { "sample": "Tissue Extracts", "range": "126 - 145 %", "average": "= 136.5" } ]

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What's included?

{ "values": { "1x96Tests": { "sellingSize": "1 x 96 Tests", "publicAssetCode":"ab100768-1x96Tests", "assetComponentDetails": [ { "size":"1 x 5 mL", "name":"2X Cell Lysis Buffer", "number":"AB100768-CMP09", "productcode":"" }, { "size":"1 x 1 Unit", "name":"IL-1 beta Microplate (12 x 8 wells)", "number":"AB100768-CMP01", "productcode":"" }, { "size":"1 x 12 mL", "name":"TMB One-Step Substrate Reagent", "number":"AB100768-CMP08", "productcode":"" }, { "size":"1 x 10 mL", "name":"5X Sample Diluent Buffer", "number":"AB100768-CMP03", "productcode":"" }, { "size":"1 x 25 mL", "name":"20X Wash Buffer", "number":"AB100768-CMP04", "productcode":"" }, { "size":"1 x 15 mL", "name":"5X Assay Diluent", "number":"AB100768-CMP06", "productcode":"" }, { "size":"1 x 8 mL", "name":"Stop Solution", "number":"AB100768-CMP07", "productcode":"" }, { "size":"2 x 1 Vial", "name":"Recombinant rat IL-1 beta Standard (lyophilized)", "number":"AB100768-CMP02", "productcode":"" }, { "size":"2 x 1 Vial", "name":"Biotinylated anti-rat IL-1 beta", "number":"AB100768-CMP10", "productcode":"" }, { "size":"1 x 200 µL", "name":"200X HRP-Streptavidin Concentrate", "number":"AB100768-CMP05", "productcode":"" } ] } } }

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Properties and storage information

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

-20°C

Appropriate long-term storage conditions

-20°C

Storage information

-20°C

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Product protocols

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Target data

Potent pro-inflammatory cytokine. Initially discovered as the major endogenous pyrogen, induces prostaglandin synthesis, neutrophil influx and activation, T-cell activation and cytokine production, B-cell activation and antibody production, and fibroblast proliferation and collagen production. Promotes Th17 differentiation of T-cells. Synergizes with IL12/interleukin-12 to induce IFNG synthesis from T-helper 1 (Th1) cells. Plays a role in angiogenesis by inducing VEGF production synergistically with TNF and IL6. Involved in transduction of inflammation downstream of pyroptosis : its mature form is specifically released in the extracellular milieu by passing through the gasdermin-D (GSDMD) pore.

See full target information Il1b

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Publications (125)

Recent publications for all applications. Explore the full list and refine your search

BMC complementary medicine and therapies 25:211 PubMed40490803

2025

Mitigation of radiation mediated testicular dysfunction by α-tocopheryl succinate: PPAR-γ related pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Heba A Gheita,Ghada M Shafey,Maha M Aziz,Noha A Fadel

Parasites & vectors 18:129 PubMed40181380

2025

Upregulation of Nrf2 attenuates Angiostrongylus cantonensis-induced parasitic meningitis in mice.

Applications

Unspecified application

Species

Unspecified reactive species

Chii-Wen Chou,Chia-Chun Huang,Ke-Min Chen,Chun-I Wang,Wan-Jing Chen,Chiung-Hung Hsu,Shih-Chan Lai,Shyun Chou,Yu-Kang Chang,Kuan-Yu Lin,Chih-Hao Chiu,Cheng-You Lu

Archivum immunologiae et therapiae experimentalis 73: PubMed40098542

2025

Isorhynchophylline Inhibits NLRP3 Inflammasome and Improves Gestational Diabetes.

Applications

Unspecified application

Species

Unspecified reactive species

Li Pu,Li Chen,Kun Yu,Yueming Zhang,Caifeng Wang,Feizhou Jiang,Jia Shi,Jingjing Meng

AMB Express 15:46 PubMed40088373

2025

Treatment of Staphylococcus aureus-infected diabetic wounds by melatonin loaded nanocarriers.

Applications

Unspecified application

Species

Unspecified reactive species

Alyaa Farid,Ayah Mohsen,Bassant Nasser,Habiba Alaa,Mariam Abdelaziz,Maryam Mustafa,Mustafa Mansour,Nourhan Adel,Salma Magdy,Salma Mohsen,Samah Adel,Sarah Ibrahim,Shaymaa Abdel-Rahman,Sohaila Mohamed,Yomna El-Karamany

Naunyn-Schmiedeberg's archives of pharmacology 398:9307-9321 PubMed39937253

2025

Targeting the sigma-1 receptor with pridopidine induces functional neurorestoration in spinal cord ischemia-reperfusion injury.

Applications

Unspecified application

Species

Unspecified reactive species

Eman Sweed,Suzan A Khodir,Shaimaa Mohamed Motawea,Hala El-Haron,Basma Abdelnaby Mostafa,Mona S Elkholy,Mohammud Salim,Doaa Z M Shebl

Frontiers in cell and developmental biology 12:1455258 PubMed39839674

2025

Relevance of the GH-VEGFB/VEGFA axis in liver grafts from brain-dead donors with alcohol-associated liver disease.

Applications

Unspecified application

Species

Unspecified reactive species

Marc Micó-Carnero,Carlos Rojano-Alfonso,Cristina Maroto-Serrat,Juan Carlos Cutrin,Araní Casillas-Ramírez,Carmen Peralta

Neurochemical research 50:30 PubMed39576344

2024

Neuroprotective Effect of Maresin-1 in Rotenone-Induced Parkinson's Disease in Rats: The Putative Role of the JAK/STAT Pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Suzan A Khodir,Eman M Sweed,Manar A Faried,Doaa M Abo Elkhair,Marwa M Khalil,Khaled Hatem Afifi,Dalia Fathy El Agamy

BMC molecular and cell biology 25:23 PubMed39425033

2024

Induction of chronic asthma up regulated the transcription of senile factors in male rats.

Applications

Unspecified application

Species

Unspecified reactive species

Majid Hassanzadeh-Khanmiri,Rana Keyhanmanesh,Reza Mosaddeghi-Heris,Aref Delkhosh,Jafar Rezaie,Sajjad Taghizadeh,Mehdi Rezai Seghin Sara,Mahdi Ahmadi

Frontiers in pharmacology 15:1466824 PubMed39372212

2024

Evaluation of the beneficial effects of a GABA-based product containing on post-inflammatory irritable bowel syndrome: a preclinical study.

Applications

Unspecified application

Species

Unspecified reactive species

Elena Lucarini,Laura Benvenuti,Clelia Di Salvo,Vanessa D'Antongiovanni,Carolina Pellegrini,Giulia Valdiserra,Clara Ciampi,Luca Antonioli,Christian Lambiase,Lorenzo Cancelli,Antonio Grosso,Lorenzo Di Cesare Mannelli,Massimo Bellini,Carla Ghelardini,Matteo Fornai

CNS neuroscience & therapeutics 30:e70038 PubMed39317458

2024

Expression of microRNA induced by postoperative delirium-like behavior is associated with long-term default mode network disruption: Sequencing and a secondary analysis of resting-state fMRI data.

Applications

Unspecified application

Species

Unspecified reactive species

Yang Liu,Huiru Feng,Huiqun Fu,Binbin Nie,Tianlong Wang

View all publications

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websiteProtocolBooklet

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Rat IL-1 beta ELISA Kit

Sandwich ELISA - Rat IL-1 beta ELISA Kit (AB100768)

Sandwich ELISA - Rat IL-1 beta ELISA Kit (AB100768)

Sandwich ELISA - Rat IL-1 beta ELISA Kit (AB100768)

Sandwich ELISA - Rat IL-1 beta ELISA Kit (AB100768)

Key facts

Detection method

Sample types

Reacts with

Assay type

Results type

Sensitivity

Range

Assay Platform

Complementary Products

Assay Kits

Assay Kits

Assay Kits

Assay Kits

Reactivity data

Product details

Precision

Recovery

What's included?

Properties and storage information

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Storage information

Product protocols

Target data

Publications (125)

Product promise