Rat IL-1 beta ELISA Kit is a single-wash 90-min Simplestep used to quantify Rat IL-1 beta with a sensitivity of 26.58 pg/ml. The assay uses a simple mix-wash-read protocol with just one incubation and one wash step.
- Colorimetric Sandwich ELISA - 450 nm readout : works on any standard plate reader
- Design your own immunoassay: we also offer the conjugation-ready antibody pair
- Cited in over 60 citations
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- Scientific reports 13:222762023Impact of occlusal contact pattern on dental stability and oromandibular system after orthodontic tooth movement in rats.Applications:Unspecified applicationReactive species:Unspecified reactive speciesMenglin Wang,Jing Wang,Xiang Jin,Dedi Liu,Huan Bian,Yantao Zhao,Yanfeng LiPubMed 38097596
- Chemical biology & drug design 103:e143862023Protective effects of baicalin on diethyl nitrosamine-induced liver cirrhosis by suppressing oxidative stress and inflammation.Applications:Unspecified applicationReactive species:Unspecified reactive speciesHui Wang,Yufei Chang,Xiao Liu,Linan Liu,Mingxi Hua,Ang LiPubMed 37923393
- Drug design, development and therapy 17:3169-31922023Network Pharmacology Analysis and Machine-Learning Models Confirmed the Ability of YiShen HuoXue Decoction to Alleviate Renal Fibrosis by Inhibiting Pyroptosis.Applications:Unspecified applicationReactive species:Unspecified reactive speciesMinChao Feng,Fang Luo,HuiMin Wu,Yushan Chen,Jinjin Zuo,Xueying Weng,Guozhong Chen,Jian ZhongPubMed 37900883
- Critical reviews in immunology 43:25-422023Study of Therapeutic Mechanisms of Puerarin against Sepsis-Induced Myocardial Injury by Integrating Network Pharmacology, Bioinformatics Analysis, and Experimental Validation.Applications:Unspecified applicationReactive species:Unspecified reactive speciesYin Li,Lei Feng,Lin Bai,Hao JiangPubMed 37824375
- Folia neuropathologica 61:291-3002023LncRNA FTX ameliorates neuropathic pain by targeting miR-320a in a rat model of chronic constriction injury.Applications:Unspecified applicationReactive species:Unspecified reactive speciesZhisheng Lu,Yijue Zhang,Yunze LiPubMed 37818689
- Foods (Basel, Switzerland) 12:2023Immune-Boosting Potentiating Properties of Hydroalcoholic Extract in Cyclophosphamide-Induced Immunosuppression in Rats.Applications:Unspecified applicationReactive species:Unspecified reactive speciesHassan Barakat,Raghad I Alkhurayji,Thamer AljutailyPubMed 37835305
- Frontiers in cell and developmental biology 11:12529422023Tanshinone IIA inhibits cardiomyocyte pyroptosis through TLR4/NF-κB p65 pathway after acute myocardial infarction.Applications:Unspecified applicationReactive species:Unspecified reactive speciesRuoning Chai,Zelin Ye,Wenjing Xue,Shuqing Shi,Yi Wei,Yuanhui Hu,Huaqin WuPubMed 37766966
- Journal of biochemical and molecular toxicology 38:e235232023Endoplasmic reticulum stress-mediated autophagy alleviates lipopolysaccharide-induced nucleus pulposus cell pyroptosis by inhibiting CHOP signaling in vitro.Applications:Unspecified applicationReactive species:Unspecified reactive speciesLu Chen,Lei Zhu,Hang Shi,Zhi-Yang Xie,Zan-Li Jiang,Zheng-Yuan Xu,Zi-Jian Zhang,Xiao-Tao WuPubMed 37654027
- Cell stress & chaperones 28:801-8092023Aucubin promotes activation of AMPK and alleviates cerebral ischemia/reperfusion injury in rats.Applications:Unspecified applicationReactive species:Unspecified reactive speciesJin-Jing Zhao,Bo Zhao,Xiao Bai,Shuang Zhang,Rui XuPubMed 37608231
- Neuroreport 34:677-6842023Adipose-derived stem cell exosomes ameliorate traumatic brain injury through the NLRP3 signaling pathway.Applications:Unspecified applicationReactive species:Unspecified reactive speciesLinjun Tang,Yong Xu,Liangwei Wang,Jingjing PanPubMed 37506308
Key facts
- Detection method
- Colorimetric
- Sample types
- Cell culture media, Citrate plasma, Serum, EDTA Plasma
- Assay type
- Sandwich (quantitative)
- Reactive species
- Rat
- Range
- 54.69 - 3500 pg/mL
- Assay time
- 1h 30m
- Sensitivity
- = 26.58 pg/mL
Reactivity data
| Application | Reactivity | Dilution info | Notes |
|---|---|---|---|
Application sELISA | Reactivity Reacts | Dilution info - | Notes - |
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Target data
Function
Potent pro-inflammatory cytokine. Initially discovered as the major endogenous pyrogen, induces prostaglandin synthesis, neutrophil influx and activation, T-cell activation and cytokine production, B-cell activation and antibody production, and fibroblast proliferation and collagen production. Promotes Th17 differentiation of T-cells. Synergizes with IL12/interleukin-12 to induce IFNG synthesis from T-helper 1 (Th1) cells. Plays a role in angiogenesis by inducing VEGF production synergistically with TNF and IL6. Involved in transduction of inflammation downstream of pyroptosis: its mature form is specifically released in the extracellular milieu by passing through the gasdermin-D (GSDMD) pore.
Alternative names
Interleukin-1 beta, IL-1 beta, Il1b
What's included?
Recommended products
Rat IL-1 beta ELISA Kit is a single-wash 90-min Simplestep used to quantify Rat IL-1 beta with a sensitivity of 26.58 pg/ml. The assay uses a simple mix-wash-read protocol with just one incubation and one wash step.
- Colorimetric Sandwich ELISA - 450 nm readout : works on any standard plate reader
- Design your own immunoassay: we also offer the conjugation-ready antibody pair
- Cited in over 60 citations
Key facts
- Detection method
- Colorimetric
- Sample types
- Cell culture media, Citrate plasma, Serum, EDTA Plasma
- Assay type
- Sandwich (quantitative)
- Reactive species
- Rat
- Range
- 54.69 - 3500 pg/mL
- Assay time
- 1h 30m
- Assay Platform
- Pre-coated microplate (12 x 8 well strips)
- Sensitivity
- = 26.58 pg/mL
Precision
Intra assay
| Sample | n | C.V. |
|---|---|---|
Sample Serum | n 8 | C.V. 3.9 |
Inter assay
| Sample | n | C.V. |
|---|---|---|
Sample Serum | n 3 | C.V. 4.3 |
Recovery
Sample specific recovery
| Sample type | Average % | Range |
|---|---|---|
Sample type Serum | Average % = 90 | Range 82 - 94 % |
Sample type EDTA Plasma | Average % = 85 | Range 81 - 88 % |
Sample type Cell culture media | Average % = 108 | Range 103 - 112 % |
Sample type Citrate plasma | Average % = 91 | Range 85 - 97 % |
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- +4°C
- Storage information
- +4°C
Notes
Rat IL-1 beta ELISA Kit (ab255730) is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of IL-1 beta protein in cell culture media, cit plasma, edta plasma, and serum. It uses our proprietary SimpleStep ELISA® technology. Quantitate Rat IL-1 beta with 26.58 pg/ml sensitivity.
SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:
- Single-wash protocol reduces assay time to 90 minutes or less
- High sensitivity, specificity and reproducibility from superior antibodies
- Fully validated in biological samples
- 96-wells plate breakable into 12 x 8 wells strips
A 384-well SimpleStep ELISA® microplate (Pre-coated 384 well Microplate SimpleStep ELISA® ab203359) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.
Interleukin-1 beta (IL-1 beta) is a 17.5 kDa cytokine protein of the Interleukin 1 family. IL-1 beta is secreted by macrophages and other cell types in response to inflammatory agents or infection. IL-1 beta plays a role in a number of cellular processes, including immune responses, bone remodeling, apoptosis and inflammatory pain hypersensitivity.
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2 product images
Sandwich ELISA - Rat IL-1 beta ELISA Kit (ab255730)
Example of rat IL-1 beta standard curve in Sample Diluent 75BP.
The IL-1 beta standard curve was prepared as described in Section 10. Raw data values are shown in the table. Background-subtracted data values (mean +/- SD) are graphed.
Sandwich ELISA - Rat IL-1 beta ELISA Kit (ab255730)
Interpolated concentrations of spike IL-1 beta in rat serum, plasma, and cell culture media with 10% fetal bovine serum samples.
The concentrations of IL-1 beta were measured in duplicates, interpolated from the IL-1 beta standard curves and corrected for sample dilution. Undiluted samples are as follows: serum 50%, plasma (citrate) 50%, plasma (EDTA) 50%, and 1640 RPMI media 50%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2).
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