Rat IL-1 beta ELISA Kit

Product details

Rat IL-1 beta ELISA Kit ab255730 is a rapid single-wash 90-min Sandwich ELISA to measure Rat IL-1 beta in cell culture media, citrate plasma, EDTA plasma, serum. This SimpleStep sensitivity is 26.58 pg/mL.

How the assay works

Rat IL-1 beta SimpleStep ELISA^®employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA^® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA^® protocol summary in the image section for further details.

Assay Specificity

Our SimpleStep ELISA^® kits use recombinant monoclonal antibodies rigorously validated to ensure the highest level of consistency and reproducibility, improved sensitivity and specificity and ease of scalability and security of supply.
Please refer to our protocol booklet for more details.

Rat IL-1 beta ELISA Kit ab255730 protocol summary

1. Mix: add samples/standards to the wells together with the capture and detector antibody cocktail. Incubate 1 hr at room temperature
2. Wash
3. Add TMB development solution - incubate for 10 min
4. Add Stop solution
5. Read the results on a plate reader at 450 nm

Design your own immunoassay

We offer the antibody pair used in this kit in a BSA and Azide-free format, ready for conjugation:

- Anti-IL-1 beta antibody [EPR16805-15] - BSA and Azide free (Capture) ab244864
- Anti-IL-1 beta antibody [EPR19779-124] - BSA and Azide free (Detector) ab244864

Interleukin-1 beta (IL-1 beta) is a 17.5 kDa cytokine protein of the Interleukin 1 family. IL-1 beta is secreted by macrophages and other cell types in response to inflammatory agents or infection. IL-1 beta plays a role in a number of cellular processes, including immune responses, bone remodeling, apoptosis and inflammatory pain hypersensitivity.