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AB100764

Rat IL-10 ELISA Kit

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(9 Publications)

Rat IL-10 ELISA Kit is a Sandwich (quantitative) ELISA for the measurement of Rat IL-10 in Rat in Biofluids, Cell Culture Media samples.

View Alternative Names

Il-10, Il10, Interleukin-10, IL-10, Cytokine synthesis inhibitory factor, CSIF

2 Images
Sandwich ELISA - Rat IL-10 ELISA Kit (AB100764)
  • sELISA

Supplier Data

Sandwich ELISA - Rat IL-10 ELISA Kit (AB100764)

Representative standard curve using ab100764.

Sandwich ELISA - Rat IL-10 ELISA Kit (AB100764)
  • sELISA

Supplier Data

Sandwich ELISA - Rat IL-10 ELISA Kit (AB100764)

Representative standard curve using ab100764.

Key facts

Detection method

Colorimetric

Sample types

Plasma, Cell culture supernatant, Serum

Reacts with

Rat

Assay type

Sandwich (quantitative)

Sensitivity

< 10 pg/mL

Range

8.23 - 6000 pg/mL

Assay Platform

Microplate

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Product details

Abcam's IL-10 Rat ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of rat IL-10 in serum, plasma, and cell culture supernatants.

This assay employs an antibody specific for rat IL-10 coated on a 96-well plate. Standards and samples are pipetted into the wells and IL-10 present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-rat IL-10 antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of IL-10 bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.

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Precision

[ { "reproducibilityType": "Inter", "sample": "Overall", "replicates": 0, "mean": null, "standardDeviation": null, "coefficientOfVariability": "< 12" }, { "reproducibilityType": "Intra", "sample": "Overall", "replicates": 0, "mean": null, "standardDeviation": null, "coefficientOfVariability": "< 10" } ]
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Recovery

[ { "sample": "Cell culture supernatant", "range": "90 - 109 %", "average": "= 98.69" }, { "sample": "Serum", "range": "84 - 104 %", "average": "= 96.75" }, { "sample": "Plasma", "range": "82 - 102 %", "average": "= 95.97" } ]
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What's included?

{ "values": { "1x96Tests": { "sellingSize": "1 x 96 Tests", "publicAssetCode":"ab100764-1x96Tests", "assetComponentDetails": [ { "size":"1 x 25 mL", "name":"20X Wash Buffer", "number":"AB100764-CMP02", "productcode":"" }, { "size":"1 x 8 mL", "name":"Stop Solution", "number":"AB100764-CMP05", "productcode":"" }, { "size":"1 x 1 Unit", "name":"IL-10 Microplate (12 strips x 8 wells)", "number":"AB100764-CMP06", "productcode":"" }, { "size":"1 x 200 µL", "name":"140X HRP-Streptavidin Concentrate", "number":"AB100764-CMP01", "productcode":"" }, { "size":"1 x 15 mL", "name":"5X Assay Diluent B", "number":"AB100764-CMP04", "productcode":"" }, { "size":"1 x 30 mL", "name":"Assay Diluent A", "number":"AB100764-CMP03", "productcode":"" }, { "size":"2 x 1 Vial", "name":"Biotinylated anti-Rat IL-10", "number":"AB100764-CMP09", "productcode":"" }, { "size":"2 x 1 Vial", "name":"Recombinant rat IL-10 Standard (lyophilized)", "number":"AB100764-CMP07", "productcode":"" }, { "size":"1 x 12 mL", "name":"TMB One-Step Substrate Reagent", "number":"AB100764-CMP08", "productcode":"" } ] } } }
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Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
-20°C
Appropriate long-term storage conditions
-20°C
Storage information
-20°C
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Product protocols

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Target data

Major immune regulatory cytokine that acts on many cells of the immune system where it has profound anti-inflammatory functions, limiting excessive tissue disruption caused by inflammation. Mechanistically, IL10 binds to its heterotetrameric receptor comprising IL10RA and IL10RB leading to JAK1 and STAT2-mediated phosphorylation of STAT3. In turn, STAT3 translocates to the nucleus where it drives expression of anti-inflammatory mediators. Targets antigen-presenting cells (APCs) such as macrophages and monocytes and inhibits their release of pro-inflammatory cytokines including granulocyte-macrophage colony-stimulating factor /GM-CSF, granulocyte colony-stimulating factor/G-CSF, IL-1 alpha, IL-1 beta, IL-6, IL-8 and TNF-alpha. Interferes also with antigen presentation by reducing the expression of MHC-class II and co-stimulatory molecules, thereby inhibiting their ability to induce T cell activation (By similarity). In addition, controls the inflammatory response of macrophages by reprogramming essential metabolic pathways including mTOR signaling (By similarity).
See full target information Il10
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Publications (9)

Recent publications for all applications. Explore the full list and refine your search

International journal of molecular sciences 23: PubMed36362063

2022

Siponimod Modulates the Reaction of Microglial Cells to Pro-Inflammatory Stimulation.

Applications

Unspecified application

Species

Unspecified reactive species

Joel Gruchot,Ferdinand Lein,Isabel Lewen,Laura Reiche,Vivien Weyers,Patrick Petzsch,Peter Göttle,Karl Köhrer,Hans-Peter Hartung,Patrick Küry,David Kremer

Radiation research 198:325-335 PubMed35904437

2022

Mitigation of Multi-Organ Radiation Injury with ACE2 Agonist Diminazene Aceturate.

Applications

Unspecified application

Species

Unspecified reactive species

Tracy Gasperetti,Guru Prasad Sharma,Anne C Frei,Lauren Pierce,Dana Veley,Nathan Szalewski,Jayashree Narayanan,Brian L Fish,Heather A Himburg

European journal of clinical microbiology & infectious diseases : official publication of the European Society of Clinical Microbiology 40:1623-1631 PubMed33666790

2021

Therapeutic efficacy of chitosan nanoparticles loaded with BCG-polysaccharide nucleic acid and ovalbumin on airway inflammation in asthmatic mice.

Applications

Unspecified application

Species

Unspecified reactive species

Wentao Yang,Zhaohui Dong,Yujing Li,Yingying Zhang,Huanqin Fu,Yanping Xie

Applied microbiology and biotechnology 103:6287-6296 PubMed31168650

2019

Prophylactic effects of probiotic Bifidobacterium spp. in the resolution of inflammation in arthritic rats.

Applications

Unspecified application

Species

Unspecified reactive species

Sajan Chandrangadhan Achi,Ramaprasad Ravichandra Talahalli,Prakash Motiram Halami

Cellular physiology and biochemistry : internation 46:890-906 PubMed29669322

2018

MicroRNA-381 Favors Repair of Nerve Injury Through Regulation of the SDF-1/CXCR4 Signaling Pathway via LRRC4 in Acute Cerebral Ischemia after Cerebral Lymphatic Blockage.

Applications

Unspecified application

Species

Unspecified reactive species

Jian-Min Piao,Wei Wu,Zhong-Xi Yang,Yu-Zhen Li,Qi Luo,Jin-Lu Yu

Evidence-based complementary and alternative medic 2017:8547257 PubMed28845184

2017

The Anti-Inflammatory Effect of Guchangzhixie-Pill by Reducing Colonic EC Cell Hyperplasia and Serotonin Availability in an Ulcerative Colitis Rat Model.

Applications

Unspecified application

Species

Unspecified reactive species

Yong Yang,Xianwei Zhu,Yifei Qin,Guihai Chen,Jing Zhou,Lu Li,Jianjun Guan,Li Ma,Yanyan Xue,Chenhui Li

European journal of nutrition 54:1161-71 PubMed25408198

2014

Lactobacillus plantarum CECT 7315/7316 intake modulates the acute and chronic innate inflammatory response.

Applications

Unspecified application

Species

Unspecified reactive species

Gemma Vilahur,Sergi López-Bernal,Sandra Camino,Guiomar Mendieta,Teresa Padró,Lina Badimon

BMC complementary and alternative medicine 14:156 PubMed24886259

2014

Polysaccharides from Liriopes Radix ameliorate streptozotocin-induced type I diabetic nephropathy via regulating NF-κB and p38 MAPK signaling pathways.

Applications

Unspecified application

Species

Unspecified reactive species

Hung-Jen Lu,Thing-Fong Tzeng,Shorong-Shii Liou,Sheng Da Lin,Ming-Chang Wu,I-Min Liu

BMC complementary and alternative medicine 12:226 PubMed23173654

2012

Wound repair and anti-inflammatory potential of Lonicera japonica in excision wound-induced rats.

Applications

Unspecified application

Species

Unspecified reactive species

Wei-Cheng Chen,Shorong-Shii Liou,Thing-Fong Tzeng,Shiow-Ling Lee,I-Min Liu
View all publications
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