AB100765

Rat IL-10 ELISA Kit

Name: Rat IL-10 ELISA Kit (ab100765) | Abcam
Brand: Abcam Limited
SKU: AB100765
Price: 780 USD
Availability: InStock
Rating: 3 (1 reviews)

(1 Review)

(30 Publications)

Rat IL-10 ELISA Kit is a Sandwich (quantitative) ELISA for the measurement of Rat IL-10 in Rat in Cell/Tissue Extracts samples.

View Alternative Names

Il-10, Il10, Interleukin-10, IL-10, Cytokine synthesis inhibitory factor, CSIF

1 Images

Sandwich ELISA - Rat IL-10 ELISA Kit (AB100765)

sELISA

Supplier Data

Sandwich ELISA - Rat IL-10 ELISA Kit (AB100765)

Representative standard curve using ab100765.

Key facts

Detection method

Colorimetric

Sample types

Cell culture extracts, Tissue Extracts

Reacts with

Rat

Assay type

Sandwich (quantitative)

Sensitivity

< 10 pg/mL

Range

8.23 - 6000 pg/mL

Assay Platform

Microplate

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }

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Product details

Abcam's IL-10 Rat ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of rat IL-10 in cell lysates and tissue lysates.

This assay employs an antibody specific for rat IL-10 coated on a 96-well plate. Standards and samples are pipetted into the wells and IL-10 present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-rat IL-10 antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of IL-10 bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.

REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.

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Precision

[ { "reproducibilityType": "Inter", "sample": "Overall", "replicates": 0, "mean": null, "standardDeviation": null, "coefficientOfVariability": "< 12" }, { "reproducibilityType": "Intra", "sample": "Overall", "replicates": 0, "mean": null, "standardDeviation": null, "coefficientOfVariability": "< 10" } ]

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Recovery

[ { "sample": "Cell culture extracts", "range": "133 - 149 %", "average": "= 142.1" }, { "sample": "Tissue Extracts", "range": "127 - 146 %", "average": "= 138.7" } ]

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What's included?

{ "values": { "1x96Tests": { "sellingSize": "1 x 96 Tests", "publicAssetCode":"ab100765-1x96Tests", "assetComponentDetails": [ { "size":"1 x 25 mL", "name":"20X Wash Buffer", "number":"AB100765-CMP03", "productcode":"" }, { "size":"1 x 5 mL", "name":"2X Cell Lysis Buffer", "number":"AB100765-CMP10", "productcode":"" }, { "size":"1 x 8 mL", "name":"Stop Solution", "number":"AB100765-CMP05", "productcode":"" }, { "size":"1 x 200 µL", "name":"140X HRP-Streptavidin Concentrate", "number":"AB100765-CMP01", "productcode":"" }, { "size":"1 x 10 mL", "name":"5X Sample Diluent Buffer", "number":"AB100765-CMP02", "productcode":"" }, { "size":"1 x 1 Unit", "name":"IL-10 Microplate (12 strips x 8 wells)", "number":"AB100765-CMP06", "productcode":"" }, { "size":"1 x 15 mL", "name":"5X Assay Diluent", "number":"AB100765-CMP04", "productcode":"" }, { "size":"1 x 12 mL", "name":"TMB One-Step Substrate Reagent", "number":"AB100765-CMP08", "productcode":"" }, { "size":"2 x 1 Vial", "name":"Recombinant rat IL-10 Standard (lyophilized)", "number":"AB100765-CMP07", "productcode":"" }, { "size":"2 x 1 Vial", "name":"Biotinylated anti-Rat IL-10", "number":"AB100765-CMP09", "productcode":"" } ] } } }

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Properties and storage information

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

-20°C

Appropriate long-term storage conditions

-20°C

Storage information

-20°C

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Product protocols

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Target data

Major immune regulatory cytokine that acts on many cells of the immune system where it has profound anti-inflammatory functions, limiting excessive tissue disruption caused by inflammation. Mechanistically, IL10 binds to its heterotetrameric receptor comprising IL10RA and IL10RB leading to JAK1 and STAT2-mediated phosphorylation of STAT3. In turn, STAT3 translocates to the nucleus where it drives expression of anti-inflammatory mediators. Targets antigen-presenting cells (APCs) such as macrophages and monocytes and inhibits their release of pro-inflammatory cytokines including granulocyte-macrophage colony-stimulating factor /GM-CSF, granulocyte colony-stimulating factor/G-CSF, IL-1 alpha, IL-1 beta, IL-6, IL-8 and TNF-alpha. Interferes also with antigen presentation by reducing the expression of MHC-class II and co-stimulatory molecules, thereby inhibiting their ability to induce T cell activation (By similarity). In addition, controls the inflammatory response of macrophages by reprogramming essential metabolic pathways including mTOR signaling (By similarity).

See full target information Il10

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Publications (30)

Recent publications for all applications. Explore the full list and refine your search

Inflammopharmacology 31:1423-1436 PubMed36840885

2023

Phenolic acids-rich fraction from Ficus drupacea leaves for the prevention and treatment of ethanol-induced gastric mucosal injury in rats.

Applications

Unspecified application

Species

Unspecified reactive species

Sherien M Bakry,Asmaa F Aboul Naser,Sabri I El Negoumy,Mona E S Kassem,Essam Abdel-Sattar,Meselhy R Meselhy

International journal of molecular sciences 23: PubMed36499029

2022

Treadmill Exercise Prevents Cognitive Impairments in Adolescent Intermittent Ethanol Rats by Reducing the Excessive Activation of Microglia Cell in the Hippocampus.

Applications

Unspecified application

Species

Unspecified reactive species

Yanxia Guo,Min Yan,Li Li,Li Zhao,Yan Li

Frontiers in medicine 9:1039707 PubMed37082028

2022

Atorvastatin and ezetimibe protect against hypercholesterolemia-induced lung oxidative stress, inflammation, and fibrosis in rats.

Applications

Unspecified application

Species

Unspecified reactive species

Porrnthanate Seenak,Sarawut Kumphune,Thanit Prasitsak,Nitirut Nernpermpisooth,Wachirawadee Malakul

Life (Basel, Switzerland) 12: PubMed36295108

2022

Ameliorative Impacts of Wheat Germ Oil against Ethanol-Induced Hepatic and Renal Dysfunction in Rats: Involvement of Anti-Inflammatory, Anti-Apoptotic, and Antioxidant Signaling Pathways.

Applications

Unspecified application

Species

Unspecified reactive species

Salwa A Elgendy,Samar H Baloza,Lina Abdelhady Mohammed,Hend Elsayed Nasr,Noha Osama El-Shaer,Heba I Ghamry,Saed A Althobaiti,Mustafa Shukry,Mohamed Mohamed Soliman,Heba A Elnoury

Environmental science and pollution research international 29:75086-75100 PubMed35648353

2022

Nattokinase attenuates bisphenol A or gamma irradiation-mediated hepatic and neural toxicity by activation of Nrf2 and suppression of inflammatory mediators in rats.

Applications

Unspecified application

Species

Unspecified reactive species

Mustafa M M Elbakry,Somaya Z Mansour,Hamed Helal,Esraa S A Ahmed

Toxicology research 11:325-338 PubMed35510233

2022

The ameliorative impacts of wheat germ oil against ethanol-induced gastric ulcers: involvement of anti-inflammatory, antiapoptotic, and antioxidant activities.

Applications

Unspecified application

Species

Unspecified reactive species

Rabab Shaban El-Shafey,Samar H Baloza,Lina Abdelhady Mohammed,Hend Elsayed Nasr,Mohamed Mohamed Soliman,Heba I Ghamry,Salwa A Elgendy

Frontiers in veterinary science 9:833605 PubMed35392110

2022

Taify Pomegranate Juice (TPJ) Abrogates Acrylamide-Induced Oxidative Stress Through the Regulation of Antioxidant Activity, Inflammation, and Apoptosis-Associated Genes.

Applications

Unspecified application

Species

Unspecified reactive species

Ahmed M El-Shehawi,Samy Sayed,Mohamed M Hassan,Saad Al-Otaibi,Fayez Althobaiti,Mona M Elseehy,Mohamed Soliman

Frontiers in veterinary science 8:817183 PubMed35155650

2022

The Protective Impact of Leaf Extract From Taif Against Acrylamide-Induced Hepatic Inflammation and Oxidative Damage: The Role of Antioxidants, Cytokines, and Apoptosis-Associated Genes.

Applications

Unspecified application

Species

Unspecified reactive species

Mohamed Mohamed Soliman,Saqer S Alotaibi,Samy Sayed,Mohamed M Hassan,Fayez Althobaiti,Adil Aldhahrani,Gehan B A Youssef,Ahmed M El-Shehawi

Life (Basel, Switzerland) 12: PubMed35207511

2022

The Anti-Inflammatory, Anti-Apoptotic, and Antioxidant Effects of a Pomegranate-Peel Extract against Acrylamide-Induced Hepatotoxicity in Rats.

Applications

Unspecified application

Species

Unspecified reactive species

Samy Sayed,Saqer S Alotaibi,Ahmed M El-Shehawi,Mohamed M Hassan,Mustafa Shukry,Mohamed Alkafafy,Mohamed Mohamed Soliman

Andrologia 53:e14036 PubMed33724537

2021

Impacts of n-acetyl cysteine on gibberellic acid-induced testicular dysfunction through regulation of inflammatory cytokines, steroid and antioxidant activity.

Applications

Unspecified application

Species

Unspecified reactive species

Mohamed Mohamed Soliman,Adil Aldhahrani,Ahmed Gaber,Walaa F Alsanie,Mustafa Shukry,Wafaa Abdou Mohamed,Mohamed M M Metwally,Alaa Ahmed Mohamed

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Rat IL-10 ELISA Kit

Sandwich ELISA - Rat IL-10 ELISA Kit (AB100765)

Key facts

Detection method

Sample types

Reacts with

Assay type

Sensitivity

Range

Assay Platform

Reactivity data

Product details

Precision

Recovery

What's included?

Properties and storage information

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Storage information

Product protocols

Target data

Publications (30)

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