JavaScript is disabled in your browser. Please enable JavaScript to view this website.
AB108872

Rat IL-10 ELISA Kit

Be the first to review this product! Submit a review

|

(3 Publications)

Rat IL-10 ELISA Kit is a Sandwich (quantitative) ELISA for the measurement of Rat IL-10 in Rat in Cell Culture Media samples.

View Alternative Names

Il-10, Il10, Interleukin-10, IL-10, Cytokine synthesis inhibitory factor, CSIF

1 Images
Sandwich ELISA - Rat IL-10 ELISA Kit (AB108872)
  • sELISA

Supplier Data

Sandwich ELISA - Rat IL-10 ELISA Kit (AB108872)

Representative Standard Curve Using ab108872.

Key facts

Detection method

Colorimetric

Sample types

Cell culture supernatant

Reacts with

Rat

Assay type

Sandwich (quantitative)

Sensitivity

= 45 pg/mL

Range

0.063 - 4 ng/mL

Assay time

4h 30m

Assay Platform

Microplate

style
left-column

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
style
left-column

Product details

Abcam's IL-10 Rat in vitro ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for the quantitative measurement of IL-10 in cell culture supernatants.

An IL-10 specific antibody has been precoated onto 96-well plates and blocked. Standards or test samples are added to the wells and subsequently an IL-10 specific biotinylated detection antibody is added and then followed by washing with wash buffer. Streptavidin-Peroxidase Conjugate is added and unbound conjugates are washed away with wash buffer. TMB is then used to visualize Streptavidin-Peroxidase enzymatic reaction. TMB is catalyzed by Streptavidin-Peroxidase to produce a blue color product that changes into yellow after adding acidic stop solution. The density of yellow coloration is directly proportional to the amount of IL-10 captured in plate.

The entire kit may be stored at -20°C for long term storage before reconstitution - Avoid repeated freeze-thaw cycles.

style
left-column

Precision

[ { "reproducibilityType": "Inter", "sample": "Overall", "replicates": 0, "mean": null, "standardDeviation": null, "coefficientOfVariability": "9.9" }, { "reproducibilityType": "Intra", "sample": "Overall", "replicates": 0, "mean": null, "standardDeviation": null, "coefficientOfVariability": "4.3" } ]
style
left-column

What's included?

{ "values": { "1x96Tests": { "sellingSize": "1 x 96 Tests", "publicAssetCode":"ab108872-1x96Tests", "assetComponentDetails": [ { "size":"2 x 30 mL", "name":"20X Wash Buffer Concentrate", "number":"AB108872-CMP05", "productcode":"" }, { "size":"1 x 7 mL", "name":"Chromogen Substrate", "number":"AB108872-CMP04", "productcode":"" }, { "size":"1 x 100 µL", "name":"60X Biotinylated Rat IL-10 Antibody", "number":"AB108872-CMP01", "productcode":"" }, { "size":"3 x 1 Unit", "name":"Sealing Tapes", "number":"AB108872-CMP08", "productcode":"" }, { "size":"1 x 11 mL", "name":"Stop Solution", "number":"AB108872-CMP09", "productcode":"" }, { "size":"1 x 80 µL", "name":"100X Streptavidin-Peroxidase Conjugate", "number":"AB108872-CMP10", "productcode":"" }, { "size":"1 x 1 Unit", "name":"IL-10 Microplate (12 x 8 well strips)", "number":"AB108872-CMP06", "productcode":"" }, { "size":"1 x 30 mL", "name":"10X Diluent N Concentrate", "number":"AB108872-CMP02", "productcode":"" }, { "size":"1 x 2 mL", "name":"1X Standard Diluent", "number":"AB108872-CMP03", "productcode":"" }, { "size":"1 x 1 Vial", "name":"IL-10 Standard", "number":"AB108872-CMP07", "productcode":"" } ] } } }
style
left-column

Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
-20°C
Appropriate long-term storage conditions
Multi
Storage information
Please refer to protocols
style
left-column
style
left-column

Product protocols

style
left-column

Target data

Major immune regulatory cytokine that acts on many cells of the immune system where it has profound anti-inflammatory functions, limiting excessive tissue disruption caused by inflammation. Mechanistically, IL10 binds to its heterotetrameric receptor comprising IL10RA and IL10RB leading to JAK1 and STAT2-mediated phosphorylation of STAT3. In turn, STAT3 translocates to the nucleus where it drives expression of anti-inflammatory mediators. Targets antigen-presenting cells (APCs) such as macrophages and monocytes and inhibits their release of pro-inflammatory cytokines including granulocyte-macrophage colony-stimulating factor /GM-CSF, granulocyte colony-stimulating factor/G-CSF, IL-1 alpha, IL-1 beta, IL-6, IL-8 and TNF-alpha. Interferes also with antigen presentation by reducing the expression of MHC-class II and co-stimulatory molecules, thereby inhibiting their ability to induce T cell activation (By similarity). In addition, controls the inflammatory response of macrophages by reprogramming essential metabolic pathways including mTOR signaling (By similarity).
See full target information Il10
style
left-column

Publications (3)

Recent publications for all applications. Explore the full list and refine your search

The Journal of pharmacy and pharmacology 73:193-205 PubMed33793806

2021

Isoliquiritigenin attenuates inflammation and modulates Nrf2/caspase-3 signalling in STZ-induced aortic injury.

Applications

Unspecified application

Species

Unspecified reactive species

Sharifa Alzahrani,Eman Said,Sadeem M Ajwah,Sumayyah Y Alsharif,Khaled S El-Bayoumi,Sawsan A Zaitone,Mona Qushawy,Nehal M Elsherbiny

Molecular medicine reports 21:583-588 PubMed31974606

2020

Notch inhibitor mitigates renal ischemia‑reperfusion injury in diabetic rats.

Applications

Unspecified application

Species

Unspecified reactive species

Xiaokai Duan,Guijun Qin

International journal of clinical and experimental 8:12509-16 PubMed26722438

2015

Berberine regulates proliferation, collagen synthesis and cytokine secretion of cardiac fibroblasts via AMPK-mTOR-p70S6K signaling pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Fen Ai,Manhua Chen,Bo Yu,Yang Yang,Guizhong Xu,Feng Gui,Zhenxing Liu,Xiangyan Bai,Zhen Chen
View all publications
style
left-column
style
left-column
websiteProtocolBooklet
en
style
right-column

Complementary Products

Assay Kits

AB236712

Rat TNF alpha ELISA Kit

Recombinant|SimpleStep

ELISA - Rat TNF alpha ELISA Kit (AB236712)

  • 0
  • 0
Assay Kits

AB222503

Mouse IL-6 ELISA Kit

Recombinant|SimpleStep

Sandwich ELISA - Mouse IL-6 ELISA Kit (AB222503)

  • 5
  • 2
Assay Kits

AB255730

Rat IL-1 beta ELISA Kit

Recombinant|SimpleStep

ELISA - Rat IL-1 beta ELISA Kit (AB255730)

  • 0
  • 0
Assay Kits

AB100770

Rat IL-4 ELISA Kit

Sandwich ELISA - Rat IL-4 ELISA Kit (AB100770)

  • 0
  • 0

Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com