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AB214566

Rat IL-10 ELISA Kit

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(40 Publications)

Rat IL-10 ELISA Kit is a single-wash 90-min Simplestep used to quantify Rat IL-10 with a sensitivity of 4.89 pg/ml. The assay uses a simple mix-wash-read protocol with just one incubation and one wash step.

- Colorimetric Sandwich ELISA - 450 nm readout : works on any standard plate reader
- Design your own immunoassay: we also offer the conjugation-ready antibody pair
- Cited in over 35 citations

View Alternative Names

Il-10, Il10, Interleukin-10, IL-10, Cytokine synthesis inhibitory factor, CSIF

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ELISA - Rat IL-10 ELISA Kit (AB214566)
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Supplier Data

ELISA - Rat IL-10 ELISA Kit (AB214566)
Sandwich ELISA - Rat IL-10 ELISA Kit (AB214566)
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Lab

Sandwich ELISA - Rat IL-10 ELISA Kit (AB214566)

Rat IL-10 standard curve comparison data.

Standard curve comparison between rat IL-10 SimpleStep ELISA® kit and traditional ELISA kit from leading competitor. SimpleStep ELISA kit shows comparable sensitivity.

Sandwich ELISA - Rat IL-10 ELISA Kit (AB214566)
  • sELISA

Supplier Data

Sandwich ELISA - Rat IL-10 ELISA Kit (AB214566)

Interpolated concentrations of native IL-10 in rat cell culture supernatant samples.

The concentrations of IL-10 were measured in duplicates, interpolated from the IL-10 standard curves and corrected for sample dilution. Undiluted samples are as follows : rat PBMC stimulated with PHA (10 μg/mL, 48 hours) supernatant 50%, unstimulated PBMC supernatant 100%, and spleen stimulated with concanavalin A (5 μg/mL, 48 hours) supernatant 50%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean IL-10 concentration was determined to be 1,174 pg/mL in PBMC stimulated with PHA supernatant, 230 pg/mL in unstimulated PBMC supernatant and 789 pg/mL in spleen stimulated with concanavalin A supernatant.

Sandwich ELISA - Rat IL-10 ELISA Kit (AB214566)
  • sELISA

Supplier Data

Sandwich ELISA - Rat IL-10 ELISA Kit (AB214566)

Example of rat IL-10 standard curve in Sample Diluent NS.

Background-subtracted data values (mean +/- SD) are graphed.

Sandwich ELISA - Rat IL-10 ELISA Kit (AB214566)
  • sELISA

Supplier Data

Sandwich ELISA - Rat IL-10 ELISA Kit (AB214566)

Rat peripheral blood mononuclear cells were cultured unstimulated or stimulated with 10 µg/mL PHA.

Conditioned media was harvested after 48 hours. IL-10 was measured in 50% unstimulated and PHA stimulated PBMC supernatant. The concentrations of IL-10 were measured in duplicate, interpolated from the IL-1beta standard curves and corrected for sample dilution. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean IL-10 concentration was determined to be 1,068 pg/mL in PHA stimulated PBMC supernatant and 227 pg/mL in unstimulated PBMC supernatant.

Sandwich ELISA - Rat IL-10 ELISA Kit (AB214566)
  • sELISA

Supplier Data

Sandwich ELISA - Rat IL-10 ELISA Kit (AB214566)

Example of rat IL-10 standard curve in Sample Diluent 25BS.

Background-subtracted data values (mean +/- SD) are graphed.

Key facts

Detection method

Colorimetric

Sample types

Citrate plasma, Cell culture supernatant, Serum

Reacts with

Rat

Assay type

Sandwich (quantitative)

Sensitivity

= 4.89 pg/mL

Range

31.3 - 2000 pg/mL

Assay time

1h 30m

Assay Platform

Pre-coated microplate (12 x 8 well strips)

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Product details

Rat IL-10 ELISA Kit ab214566 is a rapid single-wash 90-min Sandwich ELISA to measure Rat IL-10 in cell culture supernatant, citrate plasma, serum. This SimpleStep sensitivity is 4.89 pg/mL.

How the assay works

Rat IL-10 SimpleStep ELISA®employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details.

Assay Specificity

Our SimpleStep ELISA® kits use recombinant monoclonal antibodies rigorously validated to ensure the highest level of consistency and reproducibility, improved sensitivity and specificity and ease of scalability and security of supply.
Please refer to our protocol booklet for more details.

Rat IL-10 ELISA Kit ab214566 protocol summary

1. Mix: add samples/standards to the wells together with the capture and detector antibody cocktail. Incubate 1 hr at room temperature
2. Wash
3. Add TMB development solution - incubate for 10 min
4. Add Stop solution
5. Read the results on a plate reader at 450 nm

Interleukin-10 is an anti-inflammatory cytokine involved in inhibition of a variety of other cytokines, including IFN-gamma, TNF, IL-2 and GM-CSF.

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Precision

[ { "reproducibilityType": "Inter", "sample": "Overall", "replicates": 3, "mean": null, "standardDeviation": null, "coefficientOfVariability": "3.4" }, { "reproducibilityType": "Intra", "sample": "Overall", "replicates": 8, "mean": null, "standardDeviation": null, "coefficientOfVariability": "5.6" } ]
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Recovery

[ { "sample": "Cell culture supernatant", "range": "84 - 109 %", "average": "= 93" }, { "sample": "Serum", "range": "93 - 94 %", "average": "= 94" }, { "sample": "Citrate plasma", "range": "85 - 89 %", "average": "= 86" } ]
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What's included?

{ "values": { "1x96Tests": { "sellingSize": "1 x 96 Tests", "publicAssetCode":"ab214566-1x96Tests", "assetComponentDetails": [ { "size":"1 x 12 mL", "name":"Stop Solution", "number":"AB214566-CMP05", "productcode":"" }, { "size":"1 x 1 Unit", "name":"Plate Seals", "number":"AB214566-CMP10", "productcode":"" }, { "size":"1 x 12 mL", "name":"TMB Development Solution", "number":"AB214566-CMP06", "productcode":"" }, { "size":"1 x 6 mL", "name":"Antibody Diluent 5BR", "number":"AB214566-CMP02", "productcode":"" }, { "size":"1 x 50 mL", "name":"Sample Diluent NS (ab193972)", "number":"AB214566-CMP03", "productcode":"" }, { "size":"1 x 20 mL", "name":"Sample Diluent 25BS", "number":"AB214566-CMP01", "productcode":"" }, { "size":"1 x 600 µL", "name":"10X Rat IL-10 Detector Antibody", "number":"AB214566-CMP08", "productcode":"" }, { "size":"1 x 1 Unit", "name":"SimpleStep Pre-Coated 96-Well Microplate (ab206978)", "number":"AB214566-CMP04", "productcode":"" }, { "size":"1 x 20 mL", "name":"10X Wash Buffer PT (ab206977)", "number":"AB214566-CMP11", "productcode":"" }, { "size":"1 x 600 µL", "name":"10X Rat IL-10 Capture Antibody", "number":"AB214566-CMP07", "productcode":"" }, { "size":"2 x 1 Vial", "name":"Rat IL-10 Lyophilized Recombinant Protein", "number":"AB214566-CMP09", "productcode":"" } ] } } }
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Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
+4°C
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Product protocols

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Target data

Major immune regulatory cytokine that acts on many cells of the immune system where it has profound anti-inflammatory functions, limiting excessive tissue disruption caused by inflammation. Mechanistically, IL10 binds to its heterotetrameric receptor comprising IL10RA and IL10RB leading to JAK1 and STAT2-mediated phosphorylation of STAT3. In turn, STAT3 translocates to the nucleus where it drives expression of anti-inflammatory mediators. Targets antigen-presenting cells (APCs) such as macrophages and monocytes and inhibits their release of pro-inflammatory cytokines including granulocyte-macrophage colony-stimulating factor /GM-CSF, granulocyte colony-stimulating factor/G-CSF, IL-1 alpha, IL-1 beta, IL-6, IL-8 and TNF-alpha. Interferes also with antigen presentation by reducing the expression of MHC-class II and co-stimulatory molecules, thereby inhibiting their ability to induce T cell activation (By similarity). In addition, controls the inflammatory response of macrophages by reprogramming essential metabolic pathways including mTOR signaling (By similarity).
See full target information Il10
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Publications (40)

Recent publications for all applications. Explore the full list and refine your search

Nutrients 17: PubMed39861396

2025

Polyphenolic Hispolon Derived from Medicinal Mushrooms of the and Genera Promotes Wound Healing in Hyperglycemia-Induced Impairments.

Applications

Unspecified application

Species

Unspecified reactive species

Yi-Shan Liu,Mei-Chou Lai,Yu-Cheng Tzeng,I-Min Liu

International journal of nanomedicine 20:771-789 PubMed39845769

2025

Micro-Electro Nanofibrous Dressings Based on PVDF-AgNPs as Wound Healing Materials to Promote Healing in Active Areas.

Applications

Unspecified application

Species

Unspecified reactive species

Tiantian Liu,Feifei Xie,Lele Geng,Ruizhe He,Mengzhe Sun,Tao Ni,Peng Xu,Chao Xing,Yinbo Peng,Ke Chen,Yong Fang

Journal of inflammation research 17:8839-8852 PubMed39564548

2024

Norisoboldine Reduces Arthritis Severity by Attenuating Inflammation, Oxidative Stress, and Extracellular Matrix Degradation in a Rat Model of Rheumatoid Arthritis.

Applications

Unspecified application

Species

Unspecified reactive species

Ying Wang,Xiangzhuo Zhao,Jingxu Wang,Xiaoli Zhu

Nutrients 16: PubMed39339761

2024

Exploring the Wound Healing Potential of Hispidin.

Applications

Unspecified application

Species

Unspecified reactive species

Yi-Shan Liu,Mei-Chou Lai,Tang-Yao Hong,I-Min Liu

International journal of chronic obstructive pulmonary disease 19:1819-1834 PubMed39140079

2024

Network Pharmacology Followed by Experimental Validation to Explore the Mechanism of Stigmasterol in Sangbaipi Decoction Regulating PI3K/Akt Signaling to Alleviate Acute Exacerbation of Chronic Obstructive Pulmonary Disease.

Applications

Unspecified application

Species

Unspecified reactive species

Haidong He,Shuihua Sun,Weihua Xu,Mingwan Zhang

Cells 13: PubMed39056770

2024

Octadecaneuropeptide, ODN, Promotes Cell Survival against 6-OHDA-Induced Oxidative Stress and Apoptosis by Modulating the Expression of miR-34b, miR-29a, and miR-21in Cultured Astrocytes.

Applications

Unspecified application

Species

Unspecified reactive species

Amine Bourzam,Yosra Hamdi,Seyma Bahdoudi,Karthi Duraisamy,Mouna El Mehdi,Magali Basille-Dugay,Omayma Dlimi,Maher Kharrat,Anne Vejux,Gérard Lizard,Taoufik Ghrairi,Benjamin Lefranc,David Vaudry,Jean A Boutin,Jérôme Leprince,Olfa Masmoudi-Kouki

Journal of neuroinflammation 21:178 PubMed39034417

2024

PEDF-34 attenuates neurological deficit and suppresses astrocyte-dependent neuroinflammation by modulating astrocyte polarization via 67LR/JNK/STAT1 signaling pathway after subarachnoid hemorrhage in rats.

Applications

Unspecified application

Species

Unspecified reactive species

Lei Wu,Yanchao Liu,Qiuguang He,Guangnan Ao,Ningbo Xu,Wangqing He,Xiao Liu,Lei Huang,Qian Yu,Hideki Kanamaru,Siyuan Dong,Shiyi Zhu,Ye Yuan,Mingyang Han,Yeping Ling,Lu Liu,Chenyu Wu,You Zhou,Prativa Sherchan,Jerry J Flores,Jiping Tang,Xionghui Chen,Xuying He,John H Zhang

Journal of personalized medicine 14: PubMed38929866

2024

Effects of NF-κB Inhibitor on Sepsis Depend on the Severity and Phase of the Animal Sepsis Model.

Applications

Unspecified application

Species

Unspecified reactive species

Ye Jin Park,Jinkun Bae,Jae-Kwang Yoo,So-Hee Ahn,Seon Young Park,Yun-Seok Kim,Min Ji Lee,Seon Young Moon,Tae Nyoung Chung,Chulhee Choi,Kyuseok Kim

Journal of ginseng research 48:341-345 PubMed38707639

2024

Gintonin upregulates cytokine production and expression of NKp30, NKp44 and NKp44 related to natural killer cell activity on immunosuppressive rat.

Applications

Unspecified application

Species

Unspecified reactive species

BaiCheng Chen,Ajay Vijayakumar,Chul Park,Ulsoo Choi,Seung-Yeol Nah,Jong-Hoon Kim

Frontiers in pharmacology 15:1343936 PubMed38379903

2024

(haramonting) protects against allethrin-exposed pulmo damage in rats: mechanistic interleukins.

Applications

Unspecified application

Species

Unspecified reactive species

Putri Cahaya Situmorang,Syafruddin Ilyas,Rony Abdi Syahputra,Alexander Patera Nugraha,Mimmy Sari Syah Putri,Cheryl Grace Pratiwi Rumahorbo
View all publications
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