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AB326301

Rat IL-10 ELISA Kit, Chemiluminescent

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Rat IL-10 ELISA Kit, Chemiluminescent is a 55-min single-wash SimpleStep Ignite™ ELISA for the rapid quantification of Rat IL-10 with higher sensitivity and broader measuring range.

- Single 30 min incubation and single-wash step for results in less than an hour.
- Chemiluminescent detection allows a broad dynamic range: 2.74 - 6000 pg/ml with a sensitivity of 0.8pg/mL.
- Design your own immunoassay: we also offer the conjugation-ready antibody pair.
-Patent pending

View Alternative Names

Il-10, Il10, Interleukin-10, IL-10, Cytokine synthesis inhibitory factor, CSIF

5 Images
Sandwich ELISA - Rat IL-10 ELISA Kit, Chemiluminescent (AB326301)
  • sELISA

Lab

Sandwich ELISA - Rat IL-10 ELISA Kit, Chemiluminescent (AB326301)

SimpleStep ELISA® Ignite technology allows the formation of the antibody-antigen complex in one single step, reducing assay time to 55 minutes. Add samples or standards and antibody mix to wells all at once, incubate, wash, and add your final substrate. See protocol for a detailed step-by-step guide.

Sandwich ELISA - Rat IL-10 ELISA Kit, Chemiluminescent (AB326301)
  • sELISA

Lab

Sandwich ELISA - Rat IL-10 ELISA Kit, Chemiluminescent (AB326301)

Standard curve comparison between the Chemiluminescent Rat IL-10 SimpleStep ELISA and colorimetric Rat IL-10 SimpleStep ELISA (ab214566) in Sample Diluent NS. Background-subtracted data values (mean +/- SD) are graphed.

Sandwich ELISA - Rat IL-10 ELISA Kit, Chemiluminescent (AB326301)
  • sELISA

Lab

Sandwich ELISA - Rat IL-10 ELISA Kit, Chemiluminescent (AB326301)

Dynamic range comparison between the Chemiluminescent Rat IL-10 SimpleStep ELISA, colorimetric Rat IL-10 SimpleStep ELISA (ab214566), and leading colorimetric competitor. The Chemiluminescent SimpleStep ELISA kit shows increased sensitivity and increased dynamic range.

Sandwich ELISA - Rat IL-10 ELISA Kit, Chemiluminescent (AB326301)
  • sELISA

Supplier Data

Sandwich ELISA - Rat IL-10 ELISA Kit, Chemiluminescent (AB326301)

Rat peripheral blood mononuclear cells were cultured unstimulated or stimulated with 10 µg/mL PHA.

Conditioned media was harvested after 48 hours. IL-10 was measured in 50% unstimulated and PHA stimulated PBMC supernatant. The concentrations of IL-10 were measured in duplicate interpolated from the IL-1beta standard curves and corrected for sample dilution. The interpolated dilution factor corrected values are plotted (mean +/- SD n=2). The mean IL-10 concentration was determined to be 1068 pg/mL in PHA stimulated PBMC supernatant and 227 pg/mL in unstimulated PBMC supernatant.

Sandwich ELISA - Rat IL-10 ELISA Kit, Chemiluminescent (AB326301)
  • sELISA

Supplier Data

Sandwich ELISA - Rat IL-10 ELISA Kit, Chemiluminescent (AB326301)

Interpolated concentrations of native IL-10 in rat cell culture supernatant samples.

The concentrations of IL-10 were measured in duplicates interpolated from the IL-10 standard curves and corrected for sample dilution. Undiluted samples are as follows : rat PBMC stimulated with PHA (10 µg/mL 48 hours) supernatant 50% unstimulated PBMC supernatant 100% and spleen stimulated with concanavalin A (5 µg/mL 48 hours) supernatant 50%. The interpolated dilution factor corrected values are plotted (mean +/- SD n=2). The mean IL-10 concentration was determined to be 1174 pg/mL in PBMC stimulated with PHA supernatant 230 pg/mL in unstimulated PBMC supernatant and 789 pg/mL in spleen stimulated with concanavalin A supernatant.

Key facts

Detection method

Luminescent

Sample types

Serum, Citrate plasma, Cell culture supernatant

Reacts with

Rat

Assay type

Sandwich

Results type

Quantitative

Sensitivity

= 0.8 pg/mL

Range

2.74 - 6000 pg/mL

Assay time

55m

Assay Platform

Pre-coated microplate (12 x 8 well strips)

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Reactivity data

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Product details

Rat IL-10 ELISA Kit, Chemiluminescent (ab326301) is a rapid single-wash sandwich 55-min ELISA to measure Rat IL-10 in Serum, Plasma – Citrate, Cell Culture Supernatant with a sensitivity of 0.8 pg/mL.

How the assay works

Rat IL-10 ELISA Kit, Chemiluminescent (ab326301) SimpleStep Ignite™ ELISA employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step during a 30 min only incubation, significantly reducing assay time. By utilizing a chemiluminscent detection method this improves the sensitivity and dynamic range when compared to traditional colorimetric ELISAs.

Assay Specificity

Our SimpleStep Ignite™ ELISA kits use recombinant monoclonal antibodies rigorously validated to ensure the highest level of consistency and reproducibility, improved sensitivity and specificity and ease of scalability and security of supply.
Please refer to our protocol booklet for more details.

Rat IL-10 (ab326301) protocol summary

1. Mix : add samples / standards to the wells together with the capture and detector antibody cocktail. Incubate 30 min at room temperature
2. Wash
3. Add ChemiHRP development solution - incubate for 2 min
4. Read the results on a plate reader capable of luminescence detection

Design your own immunoassay

We offer the antibody pair used in this kit in a BSA and Azide-free format, ready for conjugation:

- Anti-rt_IL10 antibody [EPR19770-129] - BSA and Aside free (Capture) ab242599
- Anti-rt_IL10 antibody [EPR19770-182] - BSA and Aside free (Detector) ab242845
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Precision

[ { "reproducibilityType": "Intra", "sample": "Supernatant", "replicates": 8, "mean": null, "standardDeviation": null, "coefficientOfVariability": "= 5.6" }, { "reproducibilityType": "Inter", "sample": "Supernatant", "replicates": 3, "mean": null, "standardDeviation": null, "coefficientOfVariability": "= 3.4" } ]
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Recovery

[ { "sample": "Serum", "range": "93 - 94 %", "average": "= 94" }, { "sample": "Citrate plasma", "range": "85 - 89 %", "average": "= 86" }, { "sample": "Cell culture supernatant", "range": "84 - 109 %", "average": "= 93" } ]
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What's included?

{ "values": { "1x96Tests": { "sellingSize": "1 x 96 Tests", "publicAssetCode":"ab326301-1x96Tests", "assetComponentDetails": [ { "size":"1 x 20 mL", "name":"10X Wash Buffer PT (ab206977)", "number":"AB326301-CMP07", "productcode":"" }, { "size":"1 x 3 mL", "name":"ChemiHRP Reagent A", "number":"AB326301-CMP08", "productcode":"" }, { "size":"2 x 1 Vial", "name":"Rat IL-10 Lyophilized Recombinant Protein", "number":"AB326301-CMP03", "productcode":"" }, { "size":"1 x 12 mL", "name":"Sample Diluent NS (ab193972)", "number":"AB326301-CMP06", "productcode":"" }, { "size":"1 x 600 µL", "name":"10X Rat IL-10 Detector Antibody", "number":"AB326301-CMP02", "productcode":"" }, { "size":"1 x 20 mL", "name":"Sample Diluent 25BS", "number":"AB326301-CMP05", "productcode":"" }, { "size":"1 x 6 mL", "name":"Antibody Diluent 5BR", "number":"AB326301-CMP04", "productcode":"" }, { "size":"1 x 600 µL", "name":"10X Rat IL-10 Capture Antibody", "number":"AB326301-CMP01", "productcode":"" }, { "size":"1 x 1 Unit", "name":"Plate Seal", "number":"AB326301-CMP11", "productcode":"" }, { "size":"1 x 1 Unit", "name":"SimpleStep Pre-Coated Black 96-Well Microplate", "number":"AB326301-CMP10", "productcode":"" }, { "size":"1 x 3 mL", "name":"ChemiHRP Reagent B", "number":"AB326301-CMP09", "productcode":"" } ] } } }
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Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
+4°C
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Product protocols

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Target data

Major immune regulatory cytokine that acts on many cells of the immune system where it has profound anti-inflammatory functions, limiting excessive tissue disruption caused by inflammation. Mechanistically, IL10 binds to its heterotetrameric receptor comprising IL10RA and IL10RB leading to JAK1 and STAT2-mediated phosphorylation of STAT3. In turn, STAT3 translocates to the nucleus where it drives expression of anti-inflammatory mediators. Targets antigen-presenting cells (APCs) such as macrophages and monocytes and inhibits their release of pro-inflammatory cytokines including granulocyte-macrophage colony-stimulating factor /GM-CSF, granulocyte colony-stimulating factor/G-CSF, IL-1 alpha, IL-1 beta, IL-6, IL-8 and TNF. Also interferes with antigen presentation by reducing the expression of MHC-class II and co-stimulatory molecules, thereby inhibiting their ability to induce T cell activation (By similarity). In addition, controls the inflammatory response of macrophages by reprogramming essential metabolic pathways including mTOR signaling (By similarity).
See full target information Il10
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websiteProtocolBooklet
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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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