AB214028

Rat IL-17A ELISA Kit

Name: Rat IL-17A ELISA Kit (ab214028) | Abcam
Brand: Abcam Limited
SKU: AB214028
Price: 790 USD
Availability: InStock
Rating: 5 (1 reviews)

Recombinant
SimpleStep
What is this?

(1 Review)

(10 Publications)

Rat IL-17A ELISA Kit is a single-wash 90-min Simplestep used to quantify Rat IL-17A with a sensitivity of 1.1 pg/mL. The assay uses a simple mix-wash-read protocol with just one incubation and one wash step.

- Colorimetric Sandwich ELISA - 450 nm readout : works on any standard plate reader
- Different formats for different needs: 10x96 plates for bulk orders and 384-well plate for higher throughput
- Design your own immunoassay: we also offer the conjugation-ready antibody pair

View Alternative Names

Ctla8, Il17, Il17a, Interleukin-17A, IL-17, IL-17A, Cytotoxic T-lymphocyte-associated antigen 8, CTLA-8

15 Images

ELISA

Supplier Data

ELISA - Rat IL-17A ELISA Kit (AB214028)

sELISA

Supplier Data

Sandwich ELISA - Rat IL-17A ELISA Kit (AB214028)

Titration of mouse, rat and human recombinant IL-17a within the working range of the assay.

Two fold serial dilutions of rat, mouse and human recombinant IL-17a proteins were measured with this kit. Background-subtracted data values (mean +/- SD, n = 2) are graphed. O.D. values for human IL-17a protein were below background values. Note this kit reacts with mouse recombinant IL-17a protein. Note this kit does not react with human recombinant IL-17a protein.

sELISA

Supplier Data

Sandwich ELISA - Rat IL-17A ELISA Kit (AB214028)

Assay sensitivity.

The MDD was determined by calculating the mean of zero standard replicates and adding 2 standard deviations then extrapolating the corresponding concentration.

sELISA

Supplier Data

Sandwich ELISA - Rat IL-17A ELISA Kit (AB214028)

Example of rat IL-17a standard curve in Sample Diluent NS.

Background-subtracted data values (mean +/- SD) are graphed.

sELISA

Lab

Sandwich ELISA - Rat IL-17A ELISA Kit (AB214028)

Example of rat IL-17A standard curve in 96-well vs. 384-well plate. Background-subtracted data values (mean +/- SD) are graphed.

sELISA

Supplier Data

Sandwich ELISA - Rat IL-17A ELISA Kit (AB214028)

Serial dilutions of recombinant rat IL-17a homodimer, recombinant mouse IL-17f homodimer and recombinant mouse IL-17a/f heterodimer were measured with this kit.

Background-subtracted data values (n = 1) are graphed. Note that this kit is specific for IL-17a homodimer, it does not recognize IL-17f homodimer or IL-17a/f heterodimer.

sELISA

Supplier Data

Sandwich ELISA - Rat IL-17A ELISA Kit (AB214028)

Interpolated concentrations of native IL-17a in 2 days 5 μg/mL Concanavalin A treated rat spleen cell culture supernatant samples.

The concentrations of IL-17a were measured in duplicates, interpolated from the IL-17a standard curves and corrected for sample dilution. Undiluted samples are as follows : rat spleen cell culture supernatant 100%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean IL-17a concentration was determined to be 190 pg/mL in Concanavalin A treated rat spleen cell culture supernatant.

sELISA

Supplier Data

Sandwich ELISA - Rat IL-17A ELISA Kit (AB214028)

Comparison of IL-17a in unstimulated and Concanavalin A stimulated rat spleen cell supernatants.

Rat spleen cells were cultured in the absence or presence of 5 μg/mL Concanavalin A for 48 hours. The concentrations of Il-17a were measured in neat supernatant samples in duplicates and interpolated from the IL-17a standard curve. The interpolated values are plotted (mean +/- SD, n=2). The mean IL‑17a concentration was determined to be 33.2 pg/mL in unstimulated, 187 pg/mL in Concanavalin A stimulated supernatants and undetectable in media (not shown).

sELISA

Supplier Data

Sandwich ELISA - Rat IL-17A ELISA Kit (AB214028)

Example of rat IL-17A standard curve in Sample Diluent NS.

The IL-17A standard curve was prepared as described. Raw data values are shown in the table. Background-subtracted data values (mean +/- SD) are graphed

sELISA

Supplier Data

Sandwich ELISA - Rat IL-17A ELISA Kit (AB214028)

Example of rat IL-17A standard curve in Sample Diluent 75BS.

The IL-17A standard curve was prepared as described. Raw data values are shown in the table. Background-subtracted data values (mean +/- SD) are graphed.

sELISA

Supplier Data

Sandwich ELISA - Rat IL-17A ELISA Kit (AB214028)

Linearity of dilution is determined based on interpolated values from the standard curve. Linearity of dilution defines a sample concentration interval in which interpolated target concentrations are directly proportional to sample dilution.

Recombinant IL-17A was spiked into the following biological samples and diluted in a 2-fold dilution series in Sample Diluent 75BS.

100% pooled serum and plasma (citrate) samples from healthy donors was measured in duplicate. All values were below the detectable range of the assay.

sELISA

Supplier Data

Sandwich ELISA - Rat IL-17A ELISA Kit (AB214028)

Example of rat IL-17a standard curve in Sample Diluent 75BS.

Background-subtracted data values (mean +/- SD) are graphed

sELISA

Lab

Sandwich ELISA - Rat IL-17A ELISA Kit (AB214028)

Example of rat IL-17A standard curve in 96-well vs. 384-well plate. Background-subtracted data values (mean +/- SD) are graphed.

sELISA

Lab

Sandwich ELISA - Rat IL-17A ELISA Kit (AB214028)

Interpolated concentration of native IL-17A was measured in duplicate at different sample concentrations in 96-well vs. 384-well plates. Undiluted samples are 100% rat spleen supernatant treated for 2 days with 5 µg/mL Concanavalin A. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). Sample dilutions are made in Sample Diluent NS.

sELISA

Supplier Data

Sandwich ELISA - Rat IL-17A ELISA Kit (AB214028)

Native IL-17A was measured in the following biological samples in a 2-fold dilution series. Sample dilutions are made in Sample Diluent NS.

Key facts

Detection method

Colorimetric

Sample types

Citrate plasma, Cell culture supernatant, Serum

Reacts with

Rat

Assay type

Sandwich

Results type

Quantitative

Sensitivity

= 1.1 pg/mL

Range

6.25 - 400 pg/mL

Assay time

1h 30m

Assay Platform

Pre-coated microplate (12 x 8 well strips)

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }

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Product details

Rat IL-17A ELISA Kit ab214028 is a rapid single-wash 90-min sandwich ELISA kit to measure Rat IL-17A in Citrate plasma, Cell culture supernatant, and Serum. This Simplestep sensitivity is 1.1 pg/mL.

How the assay works

Rat IL-17A SimpleStep ELISA^®employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA^® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA^® protocol summary in the image section for further details.

Assay Specificity

Our SimpleStep ELISA^® kits use recombinant monoclonal antibodies rigorously validated to ensure the highest level of consistency and reproducibility, improved sensitivity and specificity and ease of scalability and security of supply.

Does not react with human IL-17A, mouse IL-17F and mouse IL-17A/F heterodimer.

Rat IL-17A ELISA Kit ab214028 protocol summary

1. Mix: add samples/standards to the wells together with the capture and detector antibody cocktail. Incubate 1 hr at room temperature
2. Wash
3. Add TMB development solution - incubate for 10 min
4. Add Stop solution
5. Read the results on a plate reader at 450 nm

How other researchers are using Rat IL-17A ELISA Kit ab214028

Rat IL-17A ELISA Kit ab214028 has been used to study the role of long non-coding RNA on decreasing serum biomarkers of sepsis ⁽¹⁾, inhibition of inflammatory response to identify therapeutic targets for rheumatoid arthritis⁽²⁾, and evaluate drug effects on opostoperative cognitive dysfunction ⁽³⁾.

References:

⁽¹⁾ Y. Zhou, et. al., 2023, PMID: 35317444
⁽²⁾ X. Hu, et. al., 2019, PMID: 31012109
⁽³⁾ X. Yu, et. al., 2020, PMID: 33200000

Design your own immunoassay

We offer the antibody pair used in this kit in a BSA and Azide-free format, ready for conjugation:

- Anti-IL-17A antibody [EPR16663-61] - BSA and Azide free (Capture) ab243009
- Anti-IL-17A antibody [EPR16663-89] - BSA and Azide free (Detector) ab243016

Related and Recommended products

Rat IL-17A ELISA Kit ab214028 is commonly used to measure IL-17A as a marker of inflammation.

Other ELISA kits to measure inflammatory markers include:

- Rat IL-10 ELISA Kit ab214566
- Rat IL-2 ELISA Kit ab221834
- Rat IL-17F ELISA Kit ab239423
- pan-HMR GSK3 beta ELISA Kit ab315067

IL-17A is a pro-inflammatory cytokine that is secreted by a subset of activated T cells. It is a disulfide-linked homodimer with both glycosylated and non-glycosylated forms. IL-17A induces stromal cells to produce pro-inflammatory and hematopoietic cytokines, and also enhances the surface expression of ICAM1/intracellular adhesion molecule 1 in fibroblasts.

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Precision

[ { "reproducibilityType": "Inter", "sample": "Overall", "replicates": 5, "mean": null, "standardDeviation": null, "coefficientOfVariability": "7" }, { "reproducibilityType": "Intra", "sample": "Overall", "replicates": 3, "mean": null, "standardDeviation": null, "coefficientOfVariability": "3" } ]

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Recovery

[ { "sample": "Serum", "range": "105 - 117 %", "average": "= 113" }, { "sample": "Citrate plasma", "range": "108 - 112 %", "average": "= 110" }, { "sample": "Cell culture media", "range": "96 - 110 %", "average": "= 101" } ]

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What's included?

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Properties and storage information

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

+4°C

Storage information

+4°C

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Product protocols

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Target data

Effector cytokine of innate and adaptive immune system involved in antimicrobial host defense and maintenance of tissue integrity. Signals via IL17RA-IL17RC heterodimeric receptor complex, triggering homotypic interaction of IL17RA and IL17RC chains with TRAF3IP2 adapter. This leads to downstream TRAF6-mediated activation of NF-kappa-B and MAPkinase pathways ultimately resulting in transcriptional activation of cytokines, chemokines, antimicrobial peptides and matrix metalloproteinases, with potential strong immune inflammation. Plays an important role in connecting T cell-mediated adaptive immunity and acute inflammatory response to destroy extracellular bacteria and fungi. As a signature effector cytokine of T-helper 17 cells (Th17), primarily induces neutrophil activation and recruitment at infection and inflammatory sites. In airway epithelium, mediates neutrophil chemotaxis via induction of CXCL1 and CXCL5 chemokines. In secondary lymphoid organs, contributes to germinal center formation by regulating the chemotactic response of B cells to CXCL12 and CXCL13, enhancing retention of B cells within the germinal centers, B cell somatic hypermutation rate and selection toward plasma cells. Effector cytokine of a subset of gamma-delta T cells that functions as part of an inflammatory circuit downstream IL1B, TLR2 and IL23A-IL12B to promote neutrophil recruitment for efficient bacterial clearance. Effector cytokine of innate immune cells including invariant natural killer cell (iNKT) and group 3 innate lymphoid cells that mediate initial neutrophilic inflammation. Involved in the maintenance of the integrity of epithelial barriers during homeostasis and pathogen infection. Upon acute injury, has a direct role in epithelial barrier formation by regulating OCLN localization and tight junction biogenesis. As part of the mucosal immune response induced by commensal bacteria, enhances host's ability to resist pathogenic bacterial and fungal infections by promoting neutrophil recruitment and antimicrobial peptides release. In synergy with IL17F, mediates the production of antimicrobial beta-defensins DEFB1, DEFB103A, and DEFB104A by mucosal epithelial cells, limiting the entry of microbes through the epithelial barriers. Involved in antiviral host defense through various mechanisms.

See full target information Il17a

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Publications (10)

Recent publications for all applications. Explore the full list and refine your search

AAPS PharmSciTech 26:185 PubMed40613860

2025

Novel Luteolin-Zein Nanocomposite Incorporated in Hyaluronic Acid/Sodium Alginate Scaffold as Potential Immunomodulation for Pressure Ulcer Wounds.

Applications

Unspecified application

Species

Unspecified reactive species

Nouran M Atia,Yasmine M Shahine,Ossama Y Abdallah,Mohamed S Abdel Ghany,Mona A Moustafa

Inflammopharmacology 31:1341-1359 PubMed37010718

2023

Diosmin nanocrystal gel alleviates imiquimod-induced psoriasis in rats via modulating TLR7,8/NF-κB/micro RNA-31, AKT/mTOR/P70S6K milieu, and Tregs/Th17 balance.

Applications

Unspecified application

Species

Unspecified reactive species

Yasmine Shahine,Sarah A Abd El-Aal,Ahmed M Reda,Eman Sheta,Nouran M Atia,Ossama Y Abdallah,Sherihan Salaheldin Abdelhamid Ibrahim

The EMBO journal 42:e111139 PubMed36382711

2022

Fecal microbiota transplantation enhances cell therapy in a rat model of hypoganglionosis by SCFA-induced MEK1/2 signaling pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Donghao Tian,Wenyao Xu,Weikang Pan,Baijun Zheng,Weili Yang,Wanying Jia,Yong Liu,Malgorzata A Garstka,Ya Gao,Hui Yu

Experimental and therapeutic medicine 23:279 PubMed35317444

2022

Effects and early diagnostic value of lncRNA H19 on sepsis-induced acute lung injury.

Applications

Unspecified application

Species

Unspecified reactive species

You Zhou,Liqun Sun,Mingyu Zhu,He Cheng

Pharmaceutical biology 59:1607-1618 PubMed34818128

2021

Protective effect of Qingre Huoxue decoction against myocardial infarction via PI3K/Akt autophagy pathway based on UPLC-MS, network pharmacology, and evidence.

Applications

Unspecified application

Species

Unspecified reactive species

Zheng Jin,Wenbo Zhang,Yuan Luo,Xiushen Li,Lijin Qing,Qiang Zuo,Junfeng Fang,Wei Wu

Journal of molecular endocrinology 66:233-243 PubMed33640869

2021

Inhibiting PDGF-D alleviates the symptoms of HELLP by suppressing NF-κB activation.

Applications

Unspecified application

Species

Unspecified reactive species

Haiqin Wang,Li Nian,Zhonghua Li,Changhui Lu

Experimental and therapeutic medicine 20:275 PubMed33200000

2020

Effect of dexmedetomidine combined with etomidate on IL-17A and S-100β expression levels in rats with postoperative cognitive dysfunction.

Applications

Unspecified application

Species

Unspecified reactive species

Xiaoli Yu,Yunfang Xie

RSC advances 10:3371-3379 PubMed35497743

2020

Tryptophan promoted β-defensin-2 expression the mTOR pathway and its metabolites: kynurenine banding to aryl hydrocarbon receptor in rat intestine.

Applications

Unspecified application

Species

Unspecified reactive species

Zhiru Tang,Baoshi Shi,Weizhong Sun,Yulong Yin,Qingju Chen,Taha Mohamed,Changwen Lu,Zhihong Sun

Journal of cellular physiology 234:20432-20442 PubMed31012109

2019

RGS1 silencing inhibits the inflammatory response and angiogenesis in rheumatoid arthritis rats through the inactivation of Toll-like receptor signaling pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Xumin Hu,Jianhua Tang,Gang Zeng,Xuyun Hu,Peng Bao,Jionglin Wu,Yuwei Liang,Weixi Deng,Yong Tang

Experimental and therapeutic medicine 16:4471-4478 PubMed30546393

2018

PVT1 regulates inflammation and cardiac function via the MAPK/NF-κB pathway in a sepsis model.

Applications

ELISA

Species

Rat

Fang Feng,Yan Qi,Chenming Dong,Chaohui Yang

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Rat IL-17A ELISA Kit

ELISA - Rat IL-17A ELISA Kit (AB214028)

Sandwich ELISA - Rat IL-17A ELISA Kit (AB214028)

Sandwich ELISA - Rat IL-17A ELISA Kit (AB214028)

Sandwich ELISA - Rat IL-17A ELISA Kit (AB214028)

Sandwich ELISA - Rat IL-17A ELISA Kit (AB214028)

Sandwich ELISA - Rat IL-17A ELISA Kit (AB214028)

Sandwich ELISA - Rat IL-17A ELISA Kit (AB214028)

Sandwich ELISA - Rat IL-17A ELISA Kit (AB214028)

Sandwich ELISA - Rat IL-17A ELISA Kit (AB214028)

Sandwich ELISA - Rat IL-17A ELISA Kit (AB214028)

Sandwich ELISA - Rat IL-17A ELISA Kit (AB214028)

Sandwich ELISA - Rat IL-17A ELISA Kit (AB214028)

Sandwich ELISA - Rat IL-17A ELISA Kit (AB214028)

Sandwich ELISA - Rat IL-17A ELISA Kit (AB214028)

Sandwich ELISA - Rat IL-17A ELISA Kit (AB214028)

Key facts

Detection method

Sample types

Reacts with

Assay type

Results type

Sensitivity

Range

Assay time

Assay Platform

Complementary Products

Assay Kits

Assay Kits

Secondary Antibodies

Assay Kits

Reactivity data

Product details

Precision

Recovery

What's included?

Properties and storage information

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Storage information

Product protocols

Target data

Publications (10)

Product promise