AB213909

Rat IL-18 ELISA Kit

Name: Rat IL-18 ELISA Kit (ab213909) | Abcam
Brand: Abcam Limited
SKU: AB213909
Price: 780 USD
Availability: InStock

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(25 Publications)

Rat IL-18 ELISA Kit is a Sandwich (quantitative) ELISA for the measurement of Rat IL-18 in Rat in Cell/Tissue Extracts, Cell Culture Media, Biofluids samples.

View Alternative Names

Igif, Il18, Interleukin-18, IL-18, Interferon gamma-inducing factor, Interleukin-1 gamma, IFN-gamma-inducing factor, IL-1 gamma

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Sandwich ELISA - Rat IL-18 ELISA Kit (AB213909)

sELISA

Supplier Data

Sandwich ELISA - Rat IL-18 ELISA Kit (AB213909)

Rat IL-18 ELISA Kit (ab213909) Standard Curve

Key facts

Detection method

Colorimetric

Sample types

Cell Lysate, Cell culture supernatant, EDTA Plasma, Heparin Plasma, Serum

Reacts with

Rat

Assay type

Sandwich (quantitative)

Sensitivity

< 1 pg/mL

Range

15.6 - 1000 pg/mL

Assay time

3h 30m

Assay Platform

Pre-coated microplate (12 x 8 well strips)

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }

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Product details

The Rat IL-18 Enzyme-Linked Immunosorbent Assay (ELISA) kit (ab213909) is designed for the quantitative measurement of Rat IL-18 in cell culture supernatants, serum and plasma (EDTA).

The ELISA kit is based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from mouse specific for IL-18 has been pre-coated onto 96-well plates. Standards and test samples are added to the wells; a biotinylated detection polyclonal antibody from goat specific for IL-18 is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex is added and unbound conjugates are washed away with PBS or TBS buffer. HRP substrate TMB is used to visualize HRP enzymatic reaction. TMB is catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the Rat IL-18 amount of sample captured in plate.

Interleukin (IL)-18, also called Interferon-gamma-inducing factor (IGIF), augments natural killer(NK) activity in spleen cells. The gene encodes a precursor protein of 192 amino acids and a mature protein of 157 amino acids. IL-18 is a recently discovered cytokine that modulates both T helper type 1 (Th1) and Th2 responses. IL-18 is a potent proinflammatory cytokine with potential atherogenic properties. It is highly expressed in the atherosclerotic plaques compared with control normal arteries and is localized mainly in plaque macrophages.

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Precision

[ { "reproducibilityType": "Inter", "sample": "Sample 1", "replicates": 24, "mean": "29 pg/mL", "standardDeviation": "2.2", "coefficientOfVariability": "7.6" }, { "reproducibilityType": "Inter", "sample": "Sample 2", "replicates": 24, "mean": "165 pg/mL", "standardDeviation": "9.07", "coefficientOfVariability": "5.5" }, { "reproducibilityType": "Inter", "sample": "Sample 3", "replicates": 24, "mean": "490 pg/mL", "standardDeviation": "29.4", "coefficientOfVariability": "6" }, { "reproducibilityType": "Intra", "sample": "Sample 1", "replicates": 16, "mean": "29 pg/mL", "standardDeviation": "1.97", "coefficientOfVariability": "6.8" }, { "reproducibilityType": "Intra", "sample": "Sample 2", "replicates": 16, "mean": "166 pg/mL", "standardDeviation": "2.2", "coefficientOfVariability": "4.7" }, { "reproducibilityType": "Intra", "sample": "Sample 3", "replicates": 16, "mean": "511 pg/mL", "standardDeviation": "22.99", "coefficientOfVariability": "4.5" } ]

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What's included?

{ "values": { "1x96Tests": { "sellingSize": "1 x 96 Tests", "publicAssetCode":"ab213909-1x96Tests", "assetComponentDetails": [ { "size":"1 x 100 µL", "name":"Avidin-Biotin-Peroxidase Complex (ABC)", "number":"AB213909-CMP10", "productcode":"" }, { "size":"1 x 100 µL", "name":"Biotinylated anti- Rat IL-18 antibody", "number":"AB213909-CMP08", "productcode":"" }, { "size":"1 x 1 Unit", "name":"Anti-rat IL-18 coated Microplate (12 x 8 wells)", "number":"AB213909-CMP09", "productcode":"" }, { "size":"1 x 10 mL", "name":"TMB Color Developing Agent", "number":"AB213909-CMP04", "productcode":"" }, { "size":"1 x 12 mL", "name":"Antibody Diluent Buffer", "number":"AB213909-CMP07", "productcode":"" }, { "size":"1 x 30 mL", "name":"Sample Diluent Buffer", "number":"AB213909-CMP02", "productcode":"" }, { "size":"4 x 1 Unit", "name":"Adhesive Plate Seal", "number":"AB213909-CMP06", "productcode":"" }, { "size":"1 x 10 mL", "name":"TMB Stop Solution", "number":"AB213909-CMP05", "productcode":"" }, { "size":"2 x 1 Vial", "name":"Lyophilized recombinant Rat IL-18 standard", "number":"AB213909-CMP01", "productcode":"" }, { "size":"1 x 20 mL", "name":"Wash Buffer (25X)", "number":"AB213909-CMP11", "productcode":"" }, { "size":"1 x 12 mL", "name":"ABC Diluent Buffer", "number":"AB213909-CMP03", "productcode":"" } ] } } }

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Properties and storage information

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

-20°C

Appropriate long-term storage conditions

-20°C

Storage information

-20°C

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Product protocols

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Target data

Pro-inflammatory cytokine primarily involved in epithelial barrier repair, polarized T-helper 1 (Th1) cell and natural killer (NK) cell immune responses. Upon binding to IL18R1 and IL18RAP, forms a signaling ternary complex which activates NF-kappa-B, triggering synthesis of inflammatory mediators. Synergizes with IL12/interleukin-12 to induce IFNG synthesis from T-helper 1 (Th1) cells and natural killer (NK) cells. Involved in transduction of inflammation downstream of pyroptosis : its mature form is specifically released in the extracellular milieu by passing through the gasdermin-D (GSDMD) pore.

See full target information Il18

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Publications (25)

Recent publications for all applications. Explore the full list and refine your search

Inflammopharmacology 33:3461-3477 PubMed40319428

2025

Chaperone-mediated autophagy, heat shock protein 70, and serotonin: novel targets of beta-hydroxybutyrate in HFFD/LPS-induced sporadic Alzheimer's disease model.

Applications

Unspecified application

Species

Unspecified reactive species

Reem A Mohamed,Dalaal M Abdallah,Hanan S El-Abhar

Organogenesis 21:2460261 PubMed39967390

2025

Tetramethylpyrazine Confers Protection Against Oxidative Stress and NLRP3-Dependent Pyroptosis in Rats with Endometriosis.

Applications

Unspecified application

Species

Unspecified reactive species

Ke Xu,Mingzhe Zhang,Xiaofeng Zou,Mingyang Wang

International heart journal 65:1087-1094 PubMed39537169

2024

CITED2 Attenuates Ischemia Reperfusion-Induced Pyroptosis and Injury in Cardiomyocyte.

Applications

Unspecified application

Species

Unspecified reactive species

Long Qian,Juan Zhao,Mengtao Fan,Jie Wang,Zhuqing Ji

Frontiers in pharmacology 15:1343936 PubMed38379903

2024

(haramonting) protects against allethrin-exposed pulmo damage in rats: mechanistic interleukins.

Applications

Unspecified application

Species

Unspecified reactive species

Putri Cahaya Situmorang,Syafruddin Ilyas,Rony Abdi Syahputra,Alexander Patera Nugraha,Mimmy Sari Syah Putri,Cheryl Grace Pratiwi Rumahorbo

Journal of ovarian research 17:29 PubMed38302986

2024

Bushenhuoluo Decoction improves polycystic ovary syndrome by regulating exosomal miR-30a-5p/ SOCS3/mTOR/NLRP3 signaling-mediated autophagy and pyroptosis.

Applications

Unspecified application

Species

Unspecified reactive species

Qun Huang,Yuanbin Li,Zhuang Chen,Huiping Ou,Yanjiao Tan,Hui Lin

Scientific reports 14:2511 PubMed38291200

2024

TRIM59 suppresses the brain ischaemia/reperfusion injury and pyroptosis of microglial through mediating the ubiquitination of NLRP3.

Applications

Unspecified application

Species

Unspecified reactive species

Liangtian Zhang,Gang Li,Ying Li

FASEB journal : official publication of the Federation of American Societies for Experimental Biology 38:e23382 PubMed38145344

2023

Ezetimibe ameliorates cisplatin-induced nephrotoxicity: A novel therapeutic approach via modulating AMPK/Nrf2/TXNIP signaling.

Applications

Unspecified application

Species

Unspecified reactive species

Nevine Fathy,Shaimaa Farouk,Rabab H Sayed,Atef Tadros Fahim

Frontiers in cell and developmental biology 11:1252942 PubMed37766966

2023

Tanshinone IIA inhibits cardiomyocyte pyroptosis through TLR4/NF-κB p65 pathway after acute myocardial infarction.

Applications

Unspecified application

Species

Unspecified reactive species

Ruoning Chai,Zelin Ye,Wenjing Xue,Shuqing Shi,Yi Wei,Yuanhui Hu,Huaqin Wu

Journal of biochemical and molecular toxicology 38:e23523 PubMed37654027

2023

Endoplasmic reticulum stress-mediated autophagy alleviates lipopolysaccharide-induced nucleus pulposus cell pyroptosis by inhibiting CHOP signaling in vitro.

Applications

Unspecified application

Species

Unspecified reactive species

Lu Chen,Lei Zhu,Hang Shi,Zhi-Yang Xie,Zan-Li Jiang,Zheng-Yuan Xu,Zi-Jian Zhang,Xiao-Tao Wu

Biology direct 18:23 PubMed37165439

2023

Inhibition of TRAF6 improves hyperlipidemic acute pancreatitis by alleviating pyroptosis in vitro and in vivo rat models.

Applications

Unspecified application

Species

Unspecified reactive species

Biwei Wei,Zhou Su,Huiying Yang,Yong Feng,Chunmei Meng,Zhihai Liang

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Rat IL-18 ELISA Kit

Sandwich ELISA - Rat IL-18 ELISA Kit (AB213909)

Key facts

Detection method

Sample types

Reacts with

Assay type

Sensitivity

Range

Assay time

Assay Platform

Reactivity data

Product details

Precision

What's included?

Properties and storage information

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Storage information

Product protocols

Target data

Publications (25)

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Primary Antibodies

Product promise