Rat IL-2 ELISA Kit is a single-wash 90-min Simplestep used to quantify Rat IL-2 with a sensitivity of 4.9 pg/ml. The assay uses a simple mix-wash-read protocol with just one incubation and one wash step.
- Colorimetric Sandwich ELISA - 450 nm readout : works on any standard plate reader
- Different formats for different needs: 10x96 plates for bulk orders and 384-well plate for higher throughput
- Design your own immunoassay: we also offer the conjugation-ready antibody pair
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- Anti-inflammatory & anti-allergy agents in medicinal chemistry 18:71-792018Anti-inflammatory Effect of Juniperus Procera Extract in Rats Exposed to Streptozotocin Toxicity.Applications:Unspecified applicationReactive species:Unspecified reactive speciesAdel Qlayel AlkhedaidePubMed 30474537
- Cardiology research and practice 2018:14830412018Thymoquinone Ameliorates Doxorubicin-Induced Cardiotoxicity in Swiss Albino Mice by Modulating Oxidative Damage and Cellular Inflammation.Applications:Unspecified applicationReactive species:Unspecified reactive speciesMohammad Firoz Alam et. al.PubMed 29805796
- Cellular physiology and biochemistry : internation 44:1715-17252017Reduction of Uterine Perfusion Pressure Induced Redistribution of Endothelin Receptor Type-B Between the Intima and Media Contributes to the Pathogenesis of Pregnancy-Induced Hypertension.Applications:Unspecified applicationReactive species:Unspecified reactive speciesYuan Sun et. al.PubMed 29216636
Key facts
- Detection method
- Colorimetric
- Sample types
- Cell culture media, Citrate plasma, Serum
- Assay type
- Sandwich (quantitative)
- Reactive species
- Rat
- Range
- 31.25 - 2000 pg/mL
- Assay time
- 1h 30m
- Sensitivity
- = 4.9 pg/mL
Reactivity data
| Application | Reactivity | Dilution info | Notes |
|---|---|---|---|
Application sELISA | Reactivity Reacts | Dilution info - | Notes - |
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Target data
Function
Cytokine produced by activated CD4-positive helper T-cells and to a lesser extend activated CD8-positive T-cells and natural killer (NK) cells that plays pivotal roles in the immune response and tolerance. Binds to a receptor complex composed of either the high-affinity trimeric IL-2R (IL2RA/CD25, IL2RB/CD122 and IL2RG/CD132) or the low-affinity dimeric IL-2R (IL2RB and IL2RG). Interaction with the receptor leads to oligomerization and conformation changes in the IL-2R subunits resulting in downstream signaling starting with phosphorylation of JAK1 and JAK3. In turn, JAK1 and JAK3 phosphorylate the receptor to form a docking site leading to the phosphorylation of several substrates including STAT5. This process leads to activation of several pathways including STAT, phosphoinositide-3-kinase/PI3K and mitogen-activated protein kinase/MAPK pathways. Functions as a T-cell growth factor and can increase NK-cell cytolytic activity as well. Promotes strong proliferation of activated B-cells and subsequently immunoglobulin production. Plays a pivotal role in regulating the adaptive immune system by controlling the survival and proliferation of regulatory T-cells, which are required for the maintenance of immune tolerance. Moreover, participates in the differentiation and homeostasis of effector T-cell subsets, including Th1, Th2, Th17 as well as memory CD8-positive T-cells.
Alternative names
Il-2, Il2, Interleukin-2, IL-2, T-cell growth factor, TCGF
What's included?
Recommended products
Rat IL-2 ELISA Kit is a single-wash 90-min Simplestep used to quantify Rat IL-2 with a sensitivity of 4.9 pg/ml. The assay uses a simple mix-wash-read protocol with just one incubation and one wash step.
- Colorimetric Sandwich ELISA - 450 nm readout : works on any standard plate reader
- Different formats for different needs: 10x96 plates for bulk orders and 384-well plate for higher throughput
- Design your own immunoassay: we also offer the conjugation-ready antibody pair
Key facts
- Detection method
- Colorimetric
- Sample types
- Cell culture media, Citrate plasma, Serum
- Assay type
- Sandwich (quantitative)
- Reactive species
- Rat
- Range
- 31.25 - 2000 pg/mL
- Assay time
- 1h 30m
- Assay Platform
- Microplate (12 x 8 well strips)
- Sensitivity
- = 4.9 pg/mL
Precision
Intra assay
| Sample | n | C.V. |
|---|---|---|
Sample Serum | n 3 | C.V. 4 |
Inter assay
| Sample | n | C.V. |
|---|---|---|
Sample Serum | n 5 | C.V. 4.2 |
Recovery
Sample specific recovery
| Sample type | Average % | Range |
|---|---|---|
Sample type Serum | Average % = 93 | Range 89 - 100 % |
Sample type Cell culture media | Average % = 108 | Range 99 - 113 % |
Sample type Citrate plasma | Average % = 86 | Range 84 - 88 % |
Sample type Tissue Extracts | Average % = 45 | Range 43 - 48 % |
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- +4°C
- Storage information
- +4°C
Notes
Rat IL-2 ELISA Kit (ab221834) is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of IL-2 protein in serum, cell culture media, and cit plasma. It uses our proprietary SimpleStep ELISA® technology. Quantitate Rat IL-2 with 4.9 pg/ml sensitivity.
SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:
- Single-wash protocol reduces assay time to 90 minutes or less
- High sensitivity, specificity and reproducibility from superior antibodies
- Fully validated in biological samples
- 96-wells plate breakable into 12 x 8 wells strips
A 384-well SimpleStep ELISA® microplate (Pre-coated 384 well Microplate SimpleStep ELISA® ab203359) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.
IL-2, also known as T cell growth factor (TCGF), is a glycosylated alpha-helical monomeric polypeptide. It is secreted by activated CD4+ and CD8+ T cells, neurons, microglia and hematopoietic stem cells in response to antigenic or mitogenic stimulation. IL-2 is required for T-cell proliferation, natural killer cell (NK) cytolytic activity, differentiation of regulatory T cells, modulation of T helper (Th) cell differentiation and activation-induced cell death. In particular, IL-2 modulates the expression of receptors for other cytokines and transcription factors, thereby regulating cytokine cascades that correlate with each of the Th differentiation states. Complete deficiency of IL-2 has been implicated in severe combined immunodeficiency, whereas reduction of IL-2 correlates with reduced function of CD4+CD25+ regulatory T cells and destabilization of immune homeostasis leading to autoimmune disease. Increased expression of IL-2 has also been implicated in inflammatory conditions such as inflammatory bowel disease and chronic liver diseases.
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7 product images
Sandwich ELISA - Rat IL-2 ELISA Kit (ab221834)
Example of rat IL-2 standard curve in Sample Diluent NS. Background-subtracted data values (mean +/- SD) are graphed.
ELISA - Rat IL-2 ELISA Kit (ab221834)
Example of rat IL-2 standard curve in Sample Diluent 60BS. Background-subtracted data values (mean +/- SD) are graphed.
ELISA - Rat IL-2 ELISA Kit (ab221834)
Interpolated concentrations of native IL-2 in Concanavalin A treated rat spleen (2 days) supernatant samples. The concentrations of IL-2 were measured in duplicates, interpolated from the IL-2 standard curves and corrected for sample dilution. Undiluted samples are as follows: spleen supernatant 25%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean IL-2 concentration was determined to be 6401 pg/mL in Concanavalin A treated rat spleen supernatant.
ELISA - Rat IL-2 ELISA Kit (ab221834)
Interpolated concentrations of spiked IL-2 in rat serum and plasma samples. The concentrations of IL-2 were measured in duplicates, interpolated from the IL-2 standard curves and corrected for sample dilution. Undiluted samples are as follows: serum 50%, plasma (citrate) 50%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2).
ELISA - Rat IL-2 ELISA Kit (ab221834)
Comparison of IL-2 in unstimulated and Concanavalin A stimulated rat spleen supernatants. Rat spleen was cultured for 2 days in the presence or absence of 5 μg/mL Concanavalin A. The concentrations of IL-2 were measured in three different dilutions of the supernatant samples in duplicates and interpolated from the IL-2 standard curve. The interpolated values are plotted (mean +/- SD, n=3). The mean IL-2 concentration was determined to be 6042 pg/mL in Concanavalin A stimulated neat rat spleen supernatant and undetectable in unstimulated rat spleen supernatant and RPMI media (not shown).
Sandwich ELISA - Rat IL-2 ELISA Kit (ab221834)
Example of rat IL-2 standard curve in 96-well vs. 384-well plate. Background-subtracted data values (mean +/- SD) are graphed.
Sandwich ELISA - Rat IL-2 ELISA Kit (ab221834)
Example of rat IL-2 standard curve in 96-well vs. 384-well plate. Background-subtracted data values (mean +/- SD) are graphed.
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