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AB221834

Rat IL-2 ELISA Kit

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(7 Publications)

Rat IL-2 ELISA Kit is a single-wash 90-min Simplestep used to quantify Rat IL-2 with a sensitivity of 4.9 pg/ml. The assay uses a simple mix-wash-read protocol with just one incubation and one wash step.

- Colorimetric Sandwich ELISA - 450 nm readout : works on any standard plate reader
- Different formats for different needs: 10x96 plates for bulk orders and 384-well plate for higher throughput
- Design your own immunoassay: we also offer the conjugation-ready antibody pair

View Alternative Names

Il-2, Il2, Interleukin-2, IL-2, T-cell growth factor, TCGF

8 Images
ELISA - Rat IL-2 ELISA Kit (AB221834)
  • ELISA

Supplier Data

ELISA - Rat IL-2 ELISA Kit (AB221834)
ELISA - Rat IL-2 ELISA Kit (AB221834)
  • ELISA

Supplier Data

ELISA - Rat IL-2 ELISA Kit (AB221834)

Interpolated concentrations of native IL-2 in Concanavalin A treated rat spleen (2 days) supernatant samples. The concentrations of IL-2 were measured in duplicates, interpolated from the IL-2 standard curves and corrected for sample dilution. Undiluted samples are as follows : spleen supernatant 25%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean IL-2 concentration was determined to be 6401 pg/mL in Concanavalin A treated rat spleen supernatant.

Sandwich ELISA - Rat IL-2 ELISA Kit (AB221834)
  • sELISA

Lab

Sandwich ELISA - Rat IL-2 ELISA Kit (AB221834)

Example of rat IL-2 standard curve in 96-well vs. 384-well plate. Background-subtracted data values (mean +/- SD) are graphed.

ELISA - Rat IL-2 ELISA Kit (AB221834)
  • ELISA

Supplier Data

ELISA - Rat IL-2 ELISA Kit (AB221834)

Comparison of IL-2 in unstimulated and Concanavalin A stimulated rat spleen supernatants. Rat spleen was cultured for 2 days in the presence or absence of 5 μg/mL Concanavalin A. The concentrations of IL-2 were measured in three different dilutions of the supernatant samples in duplicates and interpolated from the IL-2 standard curve. The interpolated values are plotted (mean +/- SD, n=3). The mean IL-2 concentration was determined to be 6042 pg/mL in Concanavalin A stimulated neat rat spleen supernatant and undetectable in unstimulated rat spleen supernatant and RPMI media (not shown).

Sandwich ELISA - Rat IL-2 ELISA Kit (AB221834)
  • sELISA

Lab

Sandwich ELISA - Rat IL-2 ELISA Kit (AB221834)

Example of rat IL-2 standard curve in 96-well vs. 384-well plate. Background-subtracted data values (mean +/- SD) are graphed.

ELISA - Rat IL-2 ELISA Kit (AB221834)
  • ELISA

Supplier Data

ELISA - Rat IL-2 ELISA Kit (AB221834)

Interpolated concentrations of spiked IL-2 in rat serum and plasma samples. The concentrations of IL-2 were measured in duplicates, interpolated from the IL-2 standard curves and corrected for sample dilution. Undiluted samples are as follows : serum 50%, plasma (citrate) 50%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2).

ELISA - Rat IL-2 ELISA Kit (AB221834)
  • ELISA

Supplier Data

ELISA - Rat IL-2 ELISA Kit (AB221834)

Example of rat IL-2 standard curve in Sample Diluent 60BS. Background-subtracted data values (mean +/- SD) are graphed.

Sandwich ELISA - Rat IL-2 ELISA Kit (AB221834)
  • sELISA

Supplier Data

Sandwich ELISA - Rat IL-2 ELISA Kit (AB221834)

Example of rat IL-2 standard curve in Sample Diluent NS. Background-subtracted data values (mean +/- SD) are graphed.

Key facts

Detection method

Colorimetric

Sample types

Cell culture media, Citrate plasma, Serum

Reacts with

Rat

Assay type

Sandwich

Results type

Quantitative

Sensitivity

= 4.9 pg/mL

Range

31.25 - 2000 pg/mL

Assay time

1h 30m

Assay Platform

Microplate (12 x 8 well strips)

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Product details

Rat IL-2 ELISA Kit ab221834 is a rapid single-wash 90-min Sandwich ELISA to measure Rat IL-2 in cell culture media, citrate plasma, serum. This SimpleStep sensitivity is 4.9 pg/mL.

How the assay works

Rat IL-2 SimpleStep ELISA®employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details.

Assay Specificity

Our SimpleStep ELISA® kits use recombinant monoclonal antibodies rigorously validated to ensure the highest level of consistency and reproducibility, improved sensitivity and specificity and ease of scalability and security of supply.
Please refer to our protocol booklet for more details.

Rat IL-2 ELISA Kit ab221834 protocol summary

1. Mix: add samples/standards to the wells together with the capture and detector antibody cocktail. Incubate 1 hr at room temperature
2. Wash
3. Add TMB development solution - incubate for 10 min
4. Add Stop solution
5. Read the results on a plate reader at 450 nm

Save your precious samples

Our 384-well Rat IL-2 ELISA Kit only requires a maximum of 12.5µL sample

Design your own immunoassay

We offer the antibody pair used in this kit in a BSA and Azide-free format, ready for conjugation:

- Anti-IL-2 antibody [EPR20002-281] - BSA and Azide free (Capture) ab244911
- Anti-IL-2 antibody [EPR20002-306] - BSA and Azide free (Detector) ab244911

IL-2, also known as T cell growth factor (TCGF), is a glycosylated alpha-helical monomeric polypeptide. It is secreted by activated CD4+ and CD8+ T cells, neurons, microglia and hematopoietic stem cells in response to antigenic or mitogenic stimulation. IL-2 is required for T-cell proliferation, natural killer cell (NK) cytolytic activity, differentiation of regulatory T cells, modulation of T helper (Th) cell differentiation and activation-induced cell death. In particular, IL-2 modulates the expression of receptors for other cytokines and transcription factors, thereby regulating cytokine cascades that correlate with each of the Th differentiation states. Complete deficiency of IL-2 has been implicated in severe combined immunodeficiency, whereas reduction of IL-2 correlates with reduced function of CD4+CD25+ regulatory T cells and destabilization of immune homeostasis leading to autoimmune disease. Increased expression of IL-2 has also been implicated in inflammatory conditions such as inflammatory bowel disease and chronic liver diseases.

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Precision

[ { "reproducibilityType": "Inter", "sample": "Serum", "replicates": 5, "mean": null, "standardDeviation": null, "coefficientOfVariability": "4.2" }, { "reproducibilityType": "Intra", "sample": "Serum", "replicates": 3, "mean": null, "standardDeviation": null, "coefficientOfVariability": "4" } ]
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Recovery

[ { "sample": "Serum", "range": "89 - 100 %", "average": "= 93" }, { "sample": "Cell culture media", "range": "99 - 113 %", "average": "= 108" }, { "sample": "Citrate plasma", "range": "84 - 88 %", "average": "= 86" }, { "sample": "Tissue Extracts", "range": "43 - 48 %", "average": "= 45" } ]
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What's included?

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Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
+4°C
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Product protocols

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Target data

Cytokine produced by activated CD4-positive helper T-cells and to a lesser extend activated CD8-positive T-cells and natural killer (NK) cells that plays pivotal roles in the immune response and tolerance. Binds to a receptor complex composed of either the high-affinity trimeric IL-2R (IL2RA/CD25, IL2RB/CD122 and IL2RG/CD132) or the low-affinity dimeric IL-2R (IL2RB and IL2RG). Interaction with the receptor leads to oligomerization and conformation changes in the IL-2R subunits resulting in downstream signaling starting with phosphorylation of JAK1 and JAK3. In turn, JAK1 and JAK3 phosphorylate the receptor to form a docking site leading to the phosphorylation of several substrates including STAT5. This process leads to activation of several pathways including STAT, phosphoinositide-3-kinase/PI3K and mitogen-activated protein kinase/MAPK pathways. Functions as a T-cell growth factor and can increase NK-cell cytolytic activity as well. Promotes strong proliferation of activated B-cells and subsequently immunoglobulin production. Plays a pivotal role in regulating the adaptive immune system by controlling the survival and proliferation of regulatory T-cells, which are required for the maintenance of immune tolerance. Moreover, participates in the differentiation and homeostasis of effector T-cell subsets, including Th1, Th2, Th17 as well as memory CD8-positive T-cells.
See full target information Il2
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Publications (7)

Recent publications for all applications. Explore the full list and refine your search

Basic and clinical andrology 35:9 PubMed40055615

2025

Pelvic vasectomy and its protective effects on rat testis function.

Applications

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Species

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Heng Yang,Yujun Chen,Xiaofeng Cheng,Jingxin Wu,Ruohui Huang,Biao Qian,Gongxian Wang

Current medical science 45:288-300 PubMed40035996

2025

Safranal Ameliorates Renal Damage, Inflammation, and Podocyte Injury in Membranous Nephropathy via SIRT/NF-κB Signalling.

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Yan Bao,Ya-Mei Ge,Zheng Wang,Hong-Yun Wang,Qiong Wang,Jun Yuan

Organogenesis 21:2460261 PubMed39967390

2025

Tetramethylpyrazine Confers Protection Against Oxidative Stress and NLRP3-Dependent Pyroptosis in Rats with Endometriosis.

Applications

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Species

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Ke Xu,Mingzhe Zhang,Xiaofeng Zou,Mingyang Wang

Molecular and cellular biochemistry 480:2301-2330 PubMed38941031

2024

Early-life bisphenol A exposure causes neuronal pyroptosis in juvenile and adult male rats through the NF-κB/IL-1β/NLRP3/caspase-1 signaling pathway: exploration of age and dose as effective covariates using an in vivo and in silico modeling approach.

Applications

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Species

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Ahmed S Al-Shami,Heba-Tallah Abd Elrahim Abd Elkader,Nermine Moussa,Amina E Essawy,Medhat Haroun

Anti-inflammatory & anti-allergy agents in medicinal chemistry 18:71-79 PubMed30474537

2018

Anti-inflammatory Effect of Juniperus Procera Extract in Rats Exposed to Streptozotocin Toxicity.

Applications

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Species

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Adel Qlayel Alkhedaide

Cardiology research and practice 2018:1483041 PubMed29805796

2018

Thymoquinone Ameliorates Doxorubicin-Induced Cardiotoxicity in Swiss Albino Mice by Modulating Oxidative Damage and Cellular Inflammation.

Applications

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Species

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Mohammad Firoz Alam,Gyas Khan,Mohammed M Safhi,Saeed Alshahrani,Rahimullah Siddiqui,Sivakumar Sivagurunathan Moni,Tarique Anwer

Cellular physiology and biochemistry : internation 44:1715-1725 PubMed29216636

2017

Reduction of Uterine Perfusion Pressure Induced Redistribution of Endothelin Receptor Type-B Between the Intima and Media Contributes to the Pathogenesis of Pregnancy-Induced Hypertension.

Applications

Unspecified application

Species

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Yuan Sun,Xicheng Zhang,Zhaolei Chen,Miao Xu,Minghui Ou
View all publications
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