Rat IL-3 ELISA Kit

Product details

Rat IL-3 ELISA kit (ab277709) is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of Rat IL-3 protein in human plasma (EDTA) and tissue culture supernatant. It uses our proprietary SimpleStep ELISA® technology. Quantitate Rat IL-3 with 14.5 pg/mL sensitivity.

SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:

-Single-wash protocol reduces assay time to 90 minutes or less
-High sensitivity, specificity and reproducibility from superior antibodies
-Fully validated in biological samples
-96-wells plate breakable into 12 x 8 wells strips

A 384-well SimpleStep ELISA® microplate (ab203359) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.

Interleukin 3 (IL-3) is a secreted 166 amino acids long, glycosylated interleukin coded by the IL3 gene. Previous names reported for IL-3 are Hematopoietic growth factor, Mast cell growth factor, multi-potential colony-stimulating factor, and P-cell-stimulating factor. IL-3 has been found to stimulate a variety of cell activities such as; proliferation, differentiation, survival of pluripotent hematopoietic stem cells, and may be associated with neurologic disorders. IL-3 is expressed by activated T-cells, mast cells, and natural killer cells. IL-3 performs its biological action by binding to a transmembrane receptor complex consisting of IL-3RA, also known as CD123, and a signal inducing beta subunit, which also acts as a receptor for colony stimulating factor 2 and Interleukin 5.