AB236714

Rat IL-33 ELISA Kit

Recombinant
SimpleStep
What is this?

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(2 Publications)

Rat IL-33 ELISA Kit is a single-wash 90-min Simplestep used to quantify Rat IL-33 with a sensitivity of 6 pg/ml. The assay uses a simple mix-wash-read protocol with just one incubation and one wash step.

- Colorimetric Sandwich ELISA - 450 nm readout : works on any standard plate reader
- Design your own immunoassay: we also offer the conjugation-ready antibody pair

View Alternative Names

Interleukin-33, IL-33, Il33

7 Images

Sandwich ELISA - Rat IL-33 ELISA Kit (AB236714)

sELISA

Supplier Data

Sandwich ELISA - Rat IL-33 ELISA Kit (AB236714)

Comparison of IL-33 in unstimulated and LPS stimulated lung and spleen tissue supernatants.

Lung tissue was cultured for 6 days in the presence or absence (mock) of 5 μg/mL LPS. Spleen tissue was cultured for 18 hours in the presence or absence (mock) of 1 μg/mL LPS. The concentrations of IL-33 were measured in three different dilutions of the supernatant samples in duplicates and interpolated from the IL-33 standard curve. The interpolated values are plotted (mean +/- SD, n=3). The mean IL-33 concentration was determined to be 965.8 pg/mL in LPS stimulated lung tissue culture supernatant, 940.4 in unstimulated lung tissue culture supernatant, 1,463 pg/mL in LPS stimulated spleen tissue culture supernatant, 1,165 pg/mL in unstimulated spleen tissue culture supernatant, and undetectable in RPMI1640 media containing 10% FBS (not shown).

sELISA

Supplier Data

Sandwich ELISA - Rat IL-33 ELISA Kit (AB236714)

Rat Il-33 Standard Curve Comparison.

Standard Curve comparison between Rat IL-33 SimpleStep ELISA kit and traditional ELISA kit from leading competitor. SimpleStep ELISA kit shows comparable sensitivity.

sELISA

Supplier Data

Sandwich ELISA - Rat IL-33 ELISA Kit (AB236714)

Example of rat IL-33 standard curve in Sample Diluent NS.

Background-subtracted data values (mean +/- SD) are graphed.

sELISA

Supplier Data

Sandwich ELISA - Rat IL-33 ELISA Kit (AB236714)

Example of rat IL-33 standard curve in Sample Diluent 50BP.

Background-subtracted data values (mean +/- SD) are graphed.

sELISA

Supplier Data

Sandwich ELISA - Rat IL-33 ELISA Kit (AB236714)

Example of rat IL-33 standard curve in 1X Cell Extraction Buffer PTR.

Background-subtracted data values (mean +/- SD) are graphed.

sELISA

Supplier Data

Sandwich ELISA - Rat IL-33 ELISA Kit (AB236714)

Interpolated concentrations of native IL-33 in rat lung tissue culture supernatant samples and rat spleen tissue supernatant samples.

Interpolated concentrations of native IL-33 in stimulated (LPS) and unstimulated (mock) 6 days rat lung tissue culture supernatant samples and stimulated and unstimulated 18 hours rat spleen tissue supernatant samples. The concentrations of IL-33 were measured in duplicates, interpolated from the IL-33 standard curves and corrected for sample dilution. Undiluted samples are as follows : lung (LPS) neat, lung (mock) neat, spleen (LPS) neat, and spleen (mock) neat. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean IL-33 concentration was determined to be 931.1 pg/mL in LPS stimulated lung tissue supernatant, 940.4 pg/mL in unstimulated rat lung tissue supernatant, 1,503 pg/mL in LPS stimulated spleen tissue supernatant, and 1,185 pg/mL in unstimulated spleen tissue supernatant.

sELISA

Supplier Data

Sandwich ELISA - Rat IL-33 ELISA Kit (AB236714)

Interpolated concentrations of native IL-33 in rat kidney tissue extract based on a 500 μg/mL extract load.

The concentrations of IL-33 were measured in duplicate and interpolated from the IL-33 standard curve and corrected for sample dilution. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean IL-33 concentration was determined to be 464.4 pg/mL in kidney tissue extract.

Key facts

Detection method

Colorimetric

Sample types

Tissue Extracts, Heparin Plasma, Citrate plasma, Cell culture supernatant, Serum

Reacts with

Rat

Assay type

Sandwich

Results type

Quantitative

Sensitivity

= 6 pg/mL

Range

31.3 - 3000 pg/mL

Assay time

1h 30m

Assay Platform

Pre-coated microplate (12 x 8 well strips)

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }

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Product details

Rat IL-33 ELISA Kit (ab236714) is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of IL-33 protein in cit plasma, hep plasma, serum, tissue extracts, and cell culture supernatant. It uses our proprietary SimpleStep ELISA® technology. Quantitate Rat IL-33 with 6 pg/ml sensitivity.

SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:

- Single-wash protocol reduces assay time to 90 minutes or less
- High sensitivity, specificity and reproducibility from superior antibodies
- Fully validated in biological samples
- 96-wells plate breakable into 12 x 8 wells strips

A 384-well SimpleStep ELISA® microplate (ab203359) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.

REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.

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Precision

[ { "reproducibilityType": "Inter", "sample": "Serum", "replicates": 3, "mean": null, "standardDeviation": null, "coefficientOfVariability": "3" }, { "reproducibilityType": "Intra", "sample": "Serum", "replicates": 5, "mean": null, "standardDeviation": null, "coefficientOfVariability": "2.5" } ]

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Recovery

[ { "sample": "Cell culture supernatant", "range": "106 - 123 %", "average": "= 117" }, { "sample": "Serum", "range": "98 - 101 %", "average": "= 99" }, { "sample": "Tissue Extracts", "range": "85 - 92 %", "average": "= 87" }, { "sample": "Heparin Plasma", "range": "81 - 83 %", "average": "= 82" }, { "sample": "Citrate plasma", "range": "101 - 106 %", "average": "= 103" }, { "sample": "Cell culture media", "range": "110 - 121 %", "average": "= 117" } ]

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What's included?

{ "values": { "1x96Tests": { "sellingSize": "1 x 96 Tests", "publicAssetCode":"ab236714-1x96Tests", "assetComponentDetails": [ { "size":"1 x 12 mL", "name":"Stop Solution", "number":"AB236714-CMP05", "productcode":"" }, { "size":"1 x 10 mL", "name":"5X Cell Extraction Buffer PTR (ab193970)", "number":"AB236714-CMP08", "productcode":"" }, { "size":"1 x 20 mL", "name":"10X Wash Buffer PT (ab206977)", "number":"AB236714-CMP13", "productcode":"" }, { "size":"1 x 6 mL", "name":"Antibody Diluent 5BR", "number":"AB236714-CMP01", "productcode":"" }, { "size":"1 x 12 mL", "name":"TMB Development Solution", "number":"AB236714-CMP06", "productcode":"" }, { "size":"1 x 1 Unit", "name":"Plate Seals", "number":"AB236714-CMP12", "productcode":"" }, { "size":"1 x 1 mL", "name":"50X Cell Extraction Enhancer Solution (ab193971)", "number":"AB236714-CMP07", "productcode":"" }, { "size":"1 x 600 µL", "name":"10X Rat IL-33 Detector Antibody", "number":"AB236714-CMP10", "productcode":"" }, { "size":"1 x 20 mL", "name":"Sample Diluent 50BP", "number":"AB236714-CMP02", "productcode":"" }, { "size":"1 x 50 mL", "name":"Sample Diluent NS (ab193972)", "number":"AB236714-CMP03", "productcode":"" }, { "size":"1 x 1 Unit", "name":"SimpleStep Pre-Coated 96-Well Microplate (ab206978)", "number":"AB236714-CMP04", "productcode":"" }, { "size":"1 x 600 µL", "name":"10X Rat IL-33 Capture Antibody", "number":"AB236714-CMP09", "productcode":"" }, { "size":"2 x 1 Vial", "name":"Rat IL-33 Lyophilized Recombinant Protein", "number":"AB236714-CMP11", "productcode":"" } ] } } }

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Properties and storage information

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

+4°C

Storage information

+4°C

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Product protocols

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Target data

Cytokine that binds to and signals through the IL1RL1/ST2 receptor which in turn activates NF-kappa-B and MAPK signaling pathways in target cells. Involved in the maturation of Th2 cells inducing the secretion of T-helper type 2-associated cytokines. Also involved in activation of mast cells, basophils, eosinophils and natural killer cells. Acts as a chemoattractant for Th2 cells, and may function as an 'alarmin', that amplifies immune responses during tissue injury (By similarity). Induces rapid UCP2-dependent mitochondrial rewiring that attenuates the generation of reactive oxygen species and preserves the integrity of Krebs cycle required for persistent production of itaconate and subsequent GATA3-dependent differentiation of inflammation-resolving alternatively activated macrophages (By similarity).. In quiescent endothelia the uncleaved form is constitutively and abundantly expressed, and acts as a chromatin-associated nuclear factor with transcriptional repressor properties, it may sequester nuclear NF-kappaB/RELA, lowering expression of its targets. This form is rapidely lost upon angiogenic or pro-inflammatory activation (By similarity).

See full target information Il33

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Publications (2)

Recent publications for all applications. Explore the full list and refine your search

Frontiers in pharmacology 15:1345645 PubMed38476328

2024

as a new anticancer molecular strategy in breast histology via Her2, IL33, EGFR, and MUC1.

Applications

Unspecified application

Species

Unspecified reactive species

Putri Cahaya Situmorang,Syafruddin Ilyas,Rony Abdi Syahputra,Reka Mustika Sari,Alexander Patera Nugraha,Alek Ibrahim

American journal of physiology. Heart and circulatory physiology 326:H1006-H1016 PubMed38363211

2024

IL-33 supplementation improves uterine artery resistance and maternal hypertension in response to placental ischemia.

Applications

Unspecified application

Species

Unspecified reactive species

Xi Wang,Corbin Shields,Geilda Tardo,Greg Peacock,Emily Hester,Marissa Anderson,Jan M Williams,Denise C Cornelius

View all publications

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websiteProtocolBooklet

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Rat IL-33 ELISA Kit

Sandwich ELISA - Rat IL-33 ELISA Kit (AB236714)

Sandwich ELISA - Rat IL-33 ELISA Kit (AB236714)

Sandwich ELISA - Rat IL-33 ELISA Kit (AB236714)

Sandwich ELISA - Rat IL-33 ELISA Kit (AB236714)

Sandwich ELISA - Rat IL-33 ELISA Kit (AB236714)

Sandwich ELISA - Rat IL-33 ELISA Kit (AB236714)

Sandwich ELISA - Rat IL-33 ELISA Kit (AB236714)

Key facts

Detection method

Sample types

Reacts with

Assay type

Results type

Sensitivity

Range

Assay time

Assay Platform

Complementary Products

Assay Kits

Assay Kits

Assay Kits

Primary Antibodies

Reactivity data

Product details

Precision

Recovery

What's included?

Properties and storage information

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Storage information

Product protocols

Target data

Publications (2)

Product promise