AB100770

Rat IL-4 ELISA Kit

Name: Rat IL-4 ELISA Kit (ab100770) | Abcam
Brand: Abcam Limited
SKU: AB100770
Price: 780 USD
Availability: InStock

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(12 Publications)

Rat IL-4 ELISA Kit is a Sandwich (quantitative) ELISA for the measurement of Rat IL-4 in Rat in Biofluids, Cell Culture Media samples.

View Alternative Names

Il-4, Il4, Interleukin-4, IL-4, B-cell IGG differentiation factor, B-cell growth factor 1, B-cell stimulatory factor 1, Lymphocyte stimulatory factor 1, BSF-1

2 Images

Sandwich ELISA - Rat IL-4 ELISA Kit (AB100770)

sELISA

Unknown

Sandwich ELISA - Rat IL-4 ELISA Kit (AB100770)

Representative standard curve using ab100770

sELISA

Unknown

Sandwich ELISA - Rat IL-4 ELISA Kit (AB100770)

Representative standard curve using ab100770

Key facts

Detection method

Colorimetric

Sample types

Plasma, Cell culture supernatant, Serum

Reacts with

Rat

Assay type

Sandwich (quantitative)

Sensitivity

< 1.5 pg/mL

Range

0.66 - 160 pg/mL

Assay Platform

Microplate

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }

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Product details

Abcam's IL-4 Rat ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of rat IL-4 in serum, plasma, and cell culture supernatants.

This assay employs an antibody specific for rat IL-4 coated on a 96-well plate. Standards and samples are pipetted into the wells and IL-4 present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-rat IL-4 antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of IL-4 bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.

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Recovery

[ { "sample": "Cell culture supernatant", "range": "85 - 104 %", "average": "= 97.69" }, { "sample": "Serum", "range": "84 - 103 %", "average": "= 96.79" }, { "sample": "Plasma", "range": "82 - 102 %", "average": "= 94.86" } ]

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What's included?

{ "values": { "1x96Tests": { "sellingSize": "1 x 96 Tests", "publicAssetCode":"ab100770-1x96Tests", "assetComponentDetails": [ { "size":"1 x 25 mL", "name":"20X Wash Buffer", "number":"AB100770-CMP03", "productcode":"" }, { "size":"1 x 8 mL", "name":"Stop Solution", "number":"AB100770-CMP07", "productcode":"" }, { "size":"2 x 1 Vial", "name":"Recombinant rat IL-4 Standard (lyophilized)", "number":"AB100770-CMP02", "productcode":"" }, { "size":"1 x 30 mL", "name":"Assay Diluent A", "number":"AB100770-CMP05", "productcode":"" }, { "size":"1 x 12 mL", "name":"TMB One-Step Substrate Reagent", "number":"AB100770-CMP08", "productcode":"" }, { "size":"2 x 1 Vial", "name":"Biotinylated anti-Rat IL-4", "number":"AB100770-CMP09", "productcode":"" }, { "size":"1 x 15 mL", "name":"5X Assay Diluent B", "number":"AB100770-CMP06", "productcode":"" }, { "size":"1 x 1 Unit", "name":"IL-4 Microplate (12 x 8 wells)", "number":"AB100770-CMP01", "productcode":"" }, { "size":"1 x 200 µL", "name":"600X HRP-Streptavidin Concentrate", "number":"AB100770-CMP04", "productcode":"" } ] } } }

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Properties and storage information

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

-20°C

Appropriate long-term storage conditions

-20°C

Storage information

-20°C

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Product protocols

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Target data

Participates in at least several B-cell activation processes as well as of other cell types. It is a costimulator of DNA-synthesis. It induces the expression of class II MHC molecules on resting B-cells. It enhances both secretion and cell surface expression of IgE and IgG1. It also regulates the expression of the low affinity Fc receptor for IgE (CD23) on both lymphocytes and monocytes. Positively regulates IL31RA expression in macrophages. Stimulates autophagy in dendritic cells by interfering with mTORC1 signaling and through the induction of RUFY4.

See full target information Il4

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Publications (12)

Recent publications for all applications. Explore the full list and refine your search

AMB Express 13:129 PubMed37985554

2023

Novel grape seed extract nanoparticles attenuate amikacin-induced nephrotoxicity in rats.

Applications

Unspecified application

Species

Unspecified reactive species

Alyaa Farid,Dina Mohamed,Dina Mostafa,Rawan Tarek,Viola Sherif,Gehan Safwat

Folia neuropathologica 61:291-300 PubMed37818689

2023

LncRNA FTX ameliorates neuropathic pain by targeting miR-320a in a rat model of chronic constriction injury.

Applications

Unspecified application

Species

Unspecified reactive species

Zhisheng Lu,Yijue Zhang,Yunze Li

Inflammopharmacology 31:1929-1949 PubMed37131046

2023

Fractionated whole body γ-irradiation aggravates arthritic severity via boosting NLRP3 and RANKL expression in adjuvant-induced arthritis model: the mitigative potential of ebselen.

Applications

Unspecified application

Species

Unspecified reactive species

Noura M Thabet,Mohamed K Abdel-Rafei,Mohamed M Amin

BMC psychiatry 22:419 PubMed35733107

2022

Alterations of monoamine neurotransmitters, HPA-axis hormones, and inflammation cytokines in reserpine-induced hyperalgesia and depression comorbidity rat model.

Applications

Unspecified application

Species

Unspecified reactive species

Jingjie Zhao,Wei Shi,Yujia Lu,Xuesong Gao,Anna Wang,Shan Zhang,Yi Du,Yongzhi Wang,Li Li

Evidence-based complementary and alternative medicine : eCAM 2022:3055039 PubMed35600952

2022

KGF-2 Protects against Lung Ischemia-Reperfusion Injury by Inhibiting Inflammation-Induced Damage to Endothelial Barrier Function.

Applications

Unspecified application

Species

Unspecified reactive species

Fangfang Xia,Zhousheng Jin,Jiaojiao Dong,Chaoxing Chen,Yaoyao Cai,Quanguang Wang,Hongfei Chen

Advances in clinical and experimental medicine : official organ Wroclaw Medical University 30:673-679 PubMed34118145

2021

Investigating the inflammatory cascade effect of basophil activation in children with allergic rhinitis or asthma, via the IgE-FcεRI-NF-κB signaling pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Yi Qiao,Jie Chen

Iranian journal of pharmaceutical research : IJPR 19:3-17 PubMed32922464

2020

CB2 Agonist (AM1241) Improving Effect on Ovalbumin-Induced Asthma in Rats.

Applications

Unspecified application

Species

Unspecified reactive species

Ali Parlar,Seyfullah Oktay Arslan

Nature communications 11:4504 PubMed32908131

2020

Microenvironment-responsive immunoregulatory electrospun fibers for promoting nerve function recovery.

Applications

Unspecified application

Species

Unspecified reactive species

Kun Xi,Yong Gu,Jincheng Tang,Hao Chen,Yun Xu,Liang Wu,Feng Cai,Lianfu Deng,Huilin Yang,Qin Shi,Wenguo Cui,Liang Chen

Turkish journal of surgery 36:96-104 PubMed32637881

2020

Thymoquinone reduces ischemia and reperfusion-induced intestinal injury in rats, through anti-oxidative and anti-inflammatory effects.

Applications

Unspecified application

Species

Unspecified reactive species

Ali Parlar,Seyfullah Oktay Arslan

BMC complementary and alternative medicine 19:349 PubMed31801507

2019

Immunomodulatory and anti-inflammatory effects of hydro-ethanolic extract of Ocimum basilicum leaves and its effect on lung pathological changes in an ovalbumin-induced rat model of asthma.

Applications

Unspecified application

Species

Unspecified reactive species

Naima Eftekhar,Ali Moghimi,Nema Mohammadian Roshan,Saeideh Saadat,Mohammad Hossein Boskabady

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Rat IL-4 ELISA Kit

Sandwich ELISA - Rat IL-4 ELISA Kit (AB100770)

Sandwich ELISA - Rat IL-4 ELISA Kit (AB100770)

Key facts

Detection method

Sample types

Reacts with

Assay type

Sensitivity

Range

Assay Platform

Reactivity data

Product details

Recovery

What's included?

Properties and storage information

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Storage information

Product protocols

Target data

Publications (12)

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Product promise