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AB100771

Rat IL-4 ELISA Kit

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(5 Publications)

Rat IL-4 ELISA Kit is a Sandwich (quantitative) ELISA for the measurement of Rat IL-4 in Rat in Cell/Tissue Extracts samples.

View Alternative Names

Il-4, Il4, Interleukin-4, IL-4, B-cell IGG differentiation factor, B-cell growth factor 1, B-cell stimulatory factor 1, Lymphocyte stimulatory factor 1, BSF-1

1 Images
Sandwich ELISA - Rat IL-4 ELISA Kit (AB100771)
  • sELISA

Supplier Data

Sandwich ELISA - Rat IL-4 ELISA Kit (AB100771)

Representative Standard Curve using ab100771

Key facts

Detection method

Colorimetric

Sample types

Cell culture extracts, Tissue Extracts

Reacts with

Rat

Assay type

Sandwich (quantitative)

Sensitivity

< 1.5 pg/mL

Range

0.66 - 160 pg/mL

Assay Platform

Microplate

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Product details

Abcam's IL-4 Rat ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of Rat IL-4 in cell lysates and tissue lysates.

This assay employs an antibody specific for Rat IL-4 coated on a 96- well plate. Standards and samples are pipetted into the wells and IL-4 present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-Rat IL-4 antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of IL-4 bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.

REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.

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Recovery

[ { "sample": "Cell culture extracts", "range": "85 - 105 %", "average": "= 93.49" }, { "sample": "Tissue Extracts", "range": "114 - 133 %", "average": "= 123.9" } ]
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What's included?

{ "values": { "1x96Tests": { "sellingSize": "1 x 96 Tests", "publicAssetCode":"ab100771-1x96Tests", "assetComponentDetails": [ { "size":"1 x 15 mL", "name":"5X Assay Diluent", "number":"AB100771-CMP06", "productcode":"" }, { "size":"1 x 25 mL", "name":"20X Wash Buffer", "number":"AB100771-CMP04", "productcode":"" }, { "size":"1 x 5 mL", "name":"2X Cell Lysis Buffer", "number":"AB100771-CMP10", "productcode":"" }, { "size":"1 x 8 mL", "name":"Stop Solution", "number":"AB100771-CMP07", "productcode":"" }, { "size":"2 x 1 Vial", "name":"Biotinylated anti-Rat IL-4", "number":"AB100771-CMP09", "productcode":"" }, { "size":"2 x 1 Vial", "name":"Recombinant rat IL-4 Standard (lyophilized)", "number":"AB100771-CMP03", "productcode":"" }, { "size":"1 x 200 µL", "name":"600X HRP-Streptavidin Concentrate", "number":"AB100771-CMP05", "productcode":"" }, { "size":"1 x 1 Unit", "name":"IL-4 Microplate (12 x 8 wells)", "number":"AB100771-CMP02", "productcode":"" }, { "size":"1 x 10 mL", "name":"5X Sample Diluent Buffer", "number":"AB100771-CMP01", "productcode":"" }, { "size":"1 x 12 mL", "name":"TMB One-Step Substrate Reagent", "number":"AB100771-CMP08", "productcode":"" } ] } } }
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Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
-20°C
Appropriate long-term storage conditions
-20°C
Storage information
-20°C
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Product protocols

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Target data

Participates in at least several B-cell activation processes as well as of other cell types. It is a costimulator of DNA-synthesis. It induces the expression of class II MHC molecules on resting B-cells. It enhances both secretion and cell surface expression of IgE and IgG1. It also regulates the expression of the low affinity Fc receptor for IgE (CD23) on both lymphocytes and monocytes. Positively regulates IL31RA expression in macrophages. Stimulates autophagy in dendritic cells by interfering with mTORC1 signaling and through the induction of RUFY4.
See full target information Il4
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Publications (5)

Recent publications for all applications. Explore the full list and refine your search

Frontiers in microbiology 14:1222503 PubMed37529322

2023

Comparing the therapeutic potentials of vs. against vulvovaginal candidiasis in female rats: an study.

Applications

Unspecified application

Species

Unspecified reactive species

Dalia Saad ElFeky,Alaa Reda Awad,Asmaa Mohammed Shamseldeen,Hagar Lotfy Mowafy,Sara Adel Hosny

Bioengineered 13:5509-5524 PubMed35172677

2022

Histone methyltransferase enhancer of zeste 2 polycomb repressive complex 2 subunit exacerbates inflammation in depression rats by modulating microglia polarization.

Applications

Unspecified application

Species

Unspecified reactive species

Xuezhu Huang,Qin Yang,Lingling Xie,Sihong Lei

Frontiers in aging neuroscience 12:201 PubMed32792939

2020

Strawberry Leaf Extract Treatment Alleviates Cognitive Impairment by Activating Nrf2/HO-1 Signaling in Rats With Streptozotocin-Induced Diabetes.

Applications

Unspecified application

Species

Unspecified reactive species

Li Zhang,Qinghua Ma,Yanling Zhou

European surgical research. Europaische chirurgisc 60:196-207 PubMed31694021

2019

The Mechanism of Electroacupuncture at Zusanli Promotes Macrophage Polarization during the Fibrotic Process in Contused Skeletal Muscle.

Applications

Unspecified application

Species

Unspecified reactive species

Mingyang Yan,Rongguo Wang,Shouyao Liu,Ying Chen,Peng Lin,Tengqi Li,Yunting Wang

The American journal of pathology 188:417-431 PubMed29169991

2017

Progression of Type 2 Helper T Cell-Type Inflammation and Airway Remodeling in a Rodent Model of Naturally Acquired Subclinical Primary Pneumocystis Infection.

Applications

Unspecified application

Species

Unspecified reactive species

Pablo A Iturra,Diego A Rojas,Francisco J Pérez,Andrea Méndez,Carolina A Ponce,Paula Bonilla,Rebeca Bustamante,Héctor Rodríguez,Caroll J Beltrán,Sergio L Vargas
View all publications
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Product promise

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