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AB46073

Rat IL-4 ELISA Kit

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Rat IL-4 ELISA Kit is a Sandwich (quantitative) ELISA for the measurement of Rat IL-4 in Rat in Cell Culture Media, Biofluids samples.

View Alternative Names

Il-4, Il4, Interleukin-4, IL-4, B-cell IGG differentiation factor, B-cell growth factor 1, B-cell stimulatory factor 1, Lymphocyte stimulatory factor 1, BSF-1

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Sandwich ELISA - Rat IL-4 ELISA Kit (AB46073)
  • sELISA

Supplier Data

Sandwich ELISA - Rat IL-4 ELISA Kit (AB46073)

Representative Standard Curve using ab46073

Key facts

Detection method

Colorimetric

Sample types

Cell culture supernatant, Serum

Reacts with

Rat

Assay type

Sandwich (quantitative)

Sensitivity

< 15 pg/mL

Range

31.25 - 1000 pg/mL

Assay time

4h 45m

Assay Platform

Microplate

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Product details

Abcam's IL-4 Rat in vitro ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for the quantitative measurement of IL-4 in cell culture supernatants, buffered solutions and plasma.

A monoclonal antibody specific for IL-4 has been coated onto the wells of the microtiter strips provided. Samples, including standards of known IL-4 concentRations, control specimens or unknowns are pipetted into these wells. During the first incubation, the standards or samples and a biotinylated monoclonal antibody specific for IL-4 are simultaneously incubated. After washing, the enzyme Streptavidin-HRP, that binds the biotinylated antibody is added, incubated and washed. A TMB substRate solution is added which acts on the bound enzyme to induce a colored reaction product. The intensity of this colored product is directly proportional to the concentRation of IL-4 present in the samples.

This kit will recognize both endogenous and recombinant rat IL-4.

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Precision

[ { "reproducibilityType": "Inter", "sample": "Sample A", "replicates": 6, "mean": "128.6 pg/mL", "standardDeviation": "7.6", "coefficientOfVariability": "5.9" }, { "reproducibilityType": "Inter", "sample": "Sample B", "replicates": 6, "mean": "532.2 pg/mL", "standardDeviation": "20.6", "coefficientOfVariability": "3.8" }, { "reproducibilityType": "Intra", "sample": "Sample A", "replicates": 18, "mean": "379.3 pg/mL", "standardDeviation": "13.5", "coefficientOfVariability": "2.5" }, { "reproducibilityType": "Intra", "sample": "Sample B", "replicates": 19, "mean": "532.5 pg/mL", "standardDeviation": "5.99", "coefficientOfVariability": "1.6" } ]
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What's included?

{ "values": { "2x96Tests": { "sellingSize": "2 x 96 Tests", "publicAssetCode":"ab46073-2x96Tests", "assetComponentDetails": [ { "size":"1 x 23 mL", "name":"HRP Diluent", "number":"AB46073-CMP04" }, { "size":"1 x 24 mL", "name":"Chromogen TMB Substrate Solution", "number":"AB46073-CMP08" }, { "size":"2 x 11 mL", "name":"Stop Reagent", "number":"AB46073-CMP03" }, { "size":"4 x 5 µL", "name":"Streptavidin-HRP", "number":"AB46073-CMP05" }, { "size":"2 x 1 Unit", "name":"IL-4 Microplate (12 x 8 well strips)", "number":"AB46073-CMP09" }, { "size":"2 x 400 µL", "name":"Biotinylated anti-IL-4", "number":"AB46073-CMP02" }, { "size":"2 x 10 mL", "name":"200X Wash Buffer", "number":"AB46073-CMP06" }, { "size":"1 x 25 mL", "name":"10X Standard Diluent Buffer", "number":"AB46073-CMP07" }, { "size":"1 x 13 mL", "name":"Biotinylated Antibody Diluent", "number":"AB46073-CMP01" }, { "size":"4 x 1 Vial", "name":"Rat IL-4 Standard (Lyophilized)", "number":"AB46073-CMP10" } ] }, "1x96Tests": { "sellingSize": "1 x 96 Tests", "publicAssetCode":"ab46073-1x96Tests", "assetComponentDetails": [ { "size":"1 x 12 mL", "name":"HRP Diluent", "number":"AB46073-CMP04", "productcode":"" }, { "size":"1 x 11 mL", "name":"Chromogen TMB Substrate Solution", "number":"AB46073-CMP08", "productcode":"" }, { "size":"1 x 1 Unit", "name":"IL-4 Microplate (12 x 8 well strips)", "number":"AB46073-CMP09", "productcode":"" }, { "size":"2 x 5 µL", "name":"Streptavidin-HRP", "number":"AB46073-CMP05", "productcode":"" }, { "size":"1 x 10 mL", "name":"200X Wash Buffer", "number":"AB46073-CMP06", "productcode":"" }, { "size":"1 x 11 mL", "name":"Stop Reagent", "number":"AB46073-CMP03", "productcode":"" }, { "size":"1 x 15 mL", "name":"10X Standard Diluent Buffer", "number":"AB46073-CMP07", "productcode":"" }, { "size":"1 x 400 µL", "name":"Biotinylated anti-IL-4", "number":"AB46073-CMP02", "productcode":"" }, { "size":"1 x 7.5 mL", "name":"Biotinylated Antibody Diluent", "number":"AB46073-CMP01", "productcode":"" }, { "size":"2 x 1 Vial", "name":"Rat IL-4 Standard (Lyophilized)", "number":"AB46073-CMP10", "productcode":"" } ] } } }
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Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
+4°C
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Product protocols

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Target data

Participates in at least several B-cell activation processes as well as of other cell types. It is a costimulator of DNA-synthesis. It induces the expression of class II MHC molecules on resting B-cells. It enhances both secretion and cell surface expression of IgE and IgG1. It also regulates the expression of the low affinity Fc receptor for IgE (CD23) on both lymphocytes and monocytes. Positively regulates IL31RA expression in macrophages. Stimulates autophagy in dendritic cells by interfering with mTORC1 signaling and through the induction of RUFY4.
See full target information Il4
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Product promise

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