AB267811

Rat IL-5 ELISA Kit

Recombinant
SimpleStep
What is this?

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(3 Publications)

Rat IL-5 ELISA Kit is a single-wash 90-min Simplestep used to quantify Rat IL-5 with a sensitivity of 1.38 pg/ml. The assay uses a simple mix-wash-read protocol with just one incubation and one wash step.

- Colorimetric Sandwich ELISA - 450 nm readout : works on any standard plate reader
- Design your own immunoassay: we also offer the conjugation-ready antibody pair

View Alternative Names

Il-5, Il5, Interleukin-5, IL-5, B-cell growth factor II, Cytotoxic T-lymphocyte inducer, Eosinophil differentiation factor, T-cell replacing factor, BCGF-II, TRF

2 Images

Sandwich ELISA - Rat IL-5 ELISA Kit (AB267811)

sELISA

Supplier Data

Sandwich ELISA - Rat IL-5 ELISA Kit (AB267811)

Interpolated concentrations of spiked IL-5 in rat serum and plasma samples.

The concentrations of IL-5 were measured in duplicates, interpolated from the IL-5 standard curves and corrected for sample dilution. Undiluted samples are as follows : serum 50%, plasma (citrate) 50%, plasma (heparin) 50%, and plasma (EDTA) 50%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2).

sELISA

Supplier Data

Sandwich ELISA - Rat IL-5 ELISA Kit (AB267811)

Example of rat IL-5 standard curve in Sample Diluent NS.

The IL-5 standard curve was prepared as described in Section 10. Raw data values are shown in the table. Background-subtracted data values (mean +/- SD) are graphed.

Key facts

Detection method

Colorimetric

Sample types

Heparin Plasma, Citrate plasma, Serum, EDTA Plasma

Reacts with

Rat

Assay type

Sandwich (quantitative)

Sensitivity

= 1.38 pg/mL

Range

8.59 - 550 pg/mL

Assay time

1h 30m

Assay Platform

Pre-coated microplate (12 x 8 well strips)

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }

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Product details

Rat IL-5 ELISA Kit (ab267811) is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of IL-5 protein in edta plasma, hep plasma, serum, and cit plasma. It uses our proprietary SimpleStep ELISA® technology. Quantitate Rat IL-5 with 1.38 pg/ml sensitivity.

SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:

- Single-wash protocol reduces assay time to 90 minutes or less
- High sensitivity, specificity and reproducibility from superior antibodies
- Fully validated in biological samples
- 96-wells plate breakable into 12 x 8 wells strips

A 384-well SimpleStep ELISA® microplate (ab203359) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.

Interleukin 5 (IL-5) is a small, 113 amino acid cytokine produced by T cells involved in the differentiation of B-cells to immunoglobulin secreting cells. IL-5 is a key mediator in the survival and effector functions of mature eosinophils. Due to its effects on eosinophils, IL-5 is implicated in the pathogenesis of asthma and other hypereosinophilic inflammatory conditions.

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Precision

[ { "reproducibilityType": "Intra", "sample": "Serum", "replicates": 8, "mean": null, "standardDeviation": null, "coefficientOfVariability": "3.7" } ]

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Recovery

[ { "sample": "Serum", "range": null, "average": "= 94" }, { "sample": "EDTA Plasma", "range": null, "average": "= 82" }, { "sample": "Heparin Plasma", "range": null, "average": "= 81" }, { "sample": "Citrate plasma", "range": null, "average": "= 82" } ]

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What's included?

{ "values": { "1x96Tests": { "sellingSize": "1 x 96 Tests", "publicAssetCode":"ab267811-1x96Tests", "assetComponentDetails": [ { "size":"1 x 12 mL", "name":"Stop Solution", "number":"AB267811-CMP05", "productcode":"" }, { "size":"1 x 20 mL", "name":"10X Wash Buffer PT (ab206977)", "number":"AB267811-CMP10", "productcode":"" }, { "size":"1 x 12 mL", "name":"Sample Diluent NS (ab193972)", "number":"AB267811-CMP11", "productcode":"" }, { "size":"1 x 12 mL", "name":"TMB Development Solution", "number":"AB267811-CMP06", "productcode":"" }, { "size":"1 x 1 Unit", "name":"Plate Seals", "number":"AB267811-CMP09", "productcode":"" }, { "size":"1 x 6 mL", "name":"Antibody Diluent 4BR", "number":"AB267811-CMP01", "productcode":"" }, { "size":"1 x 600 µL", "name":"10X Rat IL-5 Capture Antibody", "number":"AB267811-CMP07", "productcode":"" }, { "size":"2 x 1 Vial", "name":"Rat IL-5 Lyophilized Recombinant Protein", "number":"AB267811-CMP03", "productcode":"" }, { "size":"1 x 600 µL", "name":"10X Rat IL-5 Detector Antibody", "number":"AB267811-CMP08", "productcode":"" }, { "size":"1 x 1 Unit", "name":"SimpleStep Pre-Coated 96-Well Microplate (ab206978)", "number":"AB267811-CMP04", "productcode":"" } ] } } }

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Properties and storage information

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

+4°C

Storage information

+4°C

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Product protocols

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Target data

Homodimeric cytokine expressed predominantly by T-lymphocytes and NK cells that plays an important role in the survival, differentiation, and chemotaxis of eosinophils (PubMed : 10446387). Acts also on activated and resting B-cells to induce immunoglobulin production, growth, and differentiation (By similarity). Mechanistically, exerts its biological effects through a receptor composed of IL5RA subunit and the cytokine receptor common subunit beta/CSF2RB. Binding to the receptor leads to activation of various kinases including LYN, SYK and JAK2 and thereby propagates signals through the RAS-MAPK and JAK-STAT5 pathways respectively (By similarity).

See full target information Il5

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Publications (3)

Recent publications for all applications. Explore the full list and refine your search

Cellular & molecular biology letters 28:91 PubMed37946128

2023

Irf7 regulates the expression of Srg3 and ferroptosis axis aggravated sepsis-induced acute lung injury.

Applications

Unspecified application

Species

Unspecified reactive species

Xinyu Ling,Shiyou Wei,Dandan Ling,Siqi Cao,Rui Chang,Qiuyun Wang,Zhize Yuan

Particle and fibre toxicology 20:16 PubMed37088832

2023

Single inhalation exposure to polyamide micro and nanoplastic particles impairs vascular dilation without generating pulmonary inflammation in virgin female Sprague Dawley rats.

Applications

Unspecified application

Species

Unspecified reactive species

Chelsea M Cary,Talia N Seymore,Dilpreet Singh,Kinal N Vayas,Michael J Goedken,Samantha Adams,Marianne Polunas,Vasanthi R Sunil,Debra L Laskin,Philip Demokritou,Phoebe A Stapleton

Advances in clinical and experimental medicine : official organ Wroclaw Medical University 30:673-679 PubMed34118145

2021

Investigating the inflammatory cascade effect of basophil activation in children with allergic rhinitis or asthma, via the IgE-FcεRI-NF-κB signaling pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Yi Qiao,Jie Chen

View all publications

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websiteProtocolBooklet

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Rat IL-5 ELISA Kit

Sandwich ELISA - Rat IL-5 ELISA Kit (AB267811)

Sandwich ELISA - Rat IL-5 ELISA Kit (AB267811)

Key facts

Detection method

Sample types

Reacts with

Assay type

Sensitivity

Range

Assay time

Assay Platform

Reactivity data

Product details

Precision

Recovery

What's included?

Properties and storage information

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Storage information

Product protocols

Target data

Publications (3)

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