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Rat IL-6 ELISA Kit is a Sandwich (quantitative) ELISA kit for the measurement of Rat IL-6 in Rat in Plasma, Cell culture supernatant samples.
Alternative names=Interleukin-6, IL-6, Il-6, Il6
- Detection method
Colorimetric
- Sample types
Plasma, Cell culture supernatant
- Assay type
Sandwich (quantitative)
- Reactive species
Rat
- Range
40.96 - 10000 pg/mL
- Sensitivity
< 30 pg/mL
Reactivity data
| Application | Reactivity | Dilution info | Notes |
|---|---|---|---|
Application sELISA | Reactivity Reacts | Dilution info - | Notes - |
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Rat IL-6 ELISA Kit is a Sandwich (quantitative) ELISA kit for the measurement of Rat IL-6 in Rat in Plasma, Cell culture supernatant samples.
Alternative names=Interleukin-6, IL-6, Il-6, Il6
Key facts
- Detection method
Colorimetric
- Sample types
Plasma, Cell culture supernatant
- Assay type
Sandwich (quantitative)
- Reactive species
Rat
- Range
40.96 - 10000 pg/mL
- Assay Platform
Microplate
- Sensitivity
< 30 pg/mL
- General recovery
119%
Recovery
Sample specific recovery
| Sample type | Average % | Range |
|---|---|---|
Sample type Cell culture supernatant | Average % = 130.2 | Range 118 - 138 % |
Sample type Plasma | Average % = 122.8 | Range 109 - 138 % |
Storage
- Shipped at conditions
Blue Ice
- Appropriate short-term storage conditions
-20°C
- Appropriate long-term storage conditions
-20°C
- Storage information
-20°C
Notes
Abcam's IL-6 Rat ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for the quantitative measurement of rat IL-6 in plasma or cell culture supernatants.
This assay employs an antibody specific for Rat IL-6 coated on a 96- well plate. Standards and samples are pipetted into the wells and IL-6 present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-Rat IL-6 antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of IL-6 bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.
Due to the relatively low abundance of IL-6 in normal rat plasma, this kit may not be suitable for use with this sample type. We have not been able to detect endogenous Rat IL-6 in normal serum with ab100772, only in serum spiked with Rat IL-6.
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