AB277721

Rat IL-7 ELISA Kit

Recombinant
SimpleStep
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(1 Publication)

Rat IL-7 ELISA Kit is a single-wash 90-min Simplestep used to quantify Rat IL-7 with a sensitivity of 1.27 pg/ml. The assay uses a simple mix-wash-read protocol with just one incubation and one wash step.

- Colorimetric Sandwich ELISA - 450 nm readout : works on any standard plate reader
- Design your own immunoassay: we also offer the conjugation-ready antibody pair

View Alternative Names

Interleukin-7, IL-7, Il7

2 Images

Sandwich ELISA - Rat IL-7 ELISA Kit (AB277721)

sELISA

Supplier Data

Sandwich ELISA - Rat IL-7 ELISA Kit (AB277721)

Interpolated concentrations of native IL-7 in rat serum.

The concentrations of IL-7 were measured in duplicates, interpolated from the IL-7 standard curves and corrected for sample dilution. Undiluted samples are as follows : serum 25%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean IL-7 concentration was determined to be 251.84 pg/mL in serum.

sELISA

Supplier Data

Sandwich ELISA - Rat IL-7 ELISA Kit (AB277721)

Example of rat IL-7 standard curve in Sample Diluent NS.

The IL-7 standard curve was prepared as described in Section 10. Raw data values are shown in the table. Background-subtracted data values (mean +/- SD) are graphed.

Key facts

Detection method

Colorimetric

Sample types

Serum

Reacts with

Rat

Assay type

Sandwich

Results type

Quantitative

Sensitivity

= 1.27 pg/mL

Range

5.47 - 350 pg/mL

Assay time

1h 30m

Assay Platform

Pre-coated microplate (12 x 8 well strips)

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }

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Product details

Rat IL-7 ELISA kit (ab277704) is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of Rat IL-7 protein in rat serum. It uses our proprietary SimpleStep ELISA® technology. Quantitate Rat IL-7 with 1.27 pg/mL sensitivity.

SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:

-Single-wash protocol reduces assay time to 90 minutes or less
-High sensitivity, specificity and reproducibility from superior antibodies
-Fully validated in biological samples
-96-wells plate breakable into 12 x 8 wells strips

A 384-well SimpleStep ELISA® microplate (ab203359) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.

IL-7 is a cytokine important for B and T cell development. In humans, IL-7 and hepatocyte growth factor form a heterodimer that functions as a pre-pro-B cell growth-stimulating factor. IL-7 is found to be a cofactor for V(D)J rearrangement of the T cell receptor beta (TCRB) during early T cell development. It can be produced locally by intestinal epithelial and epithelial goblet cells and may serve as a regulatory factor for intestinal mucosal lymphocytes. Knockout studies in mice suggested that IL-7 plays an essential role in lymphoid cell survival. Rat IL-7 shares 89% and 59% sequence homology with mouse and human respectively.

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Precision

[ { "reproducibilityType": "Inter", "sample": "Serum", "replicates": 3, "mean": null, "standardDeviation": null, "coefficientOfVariability": "1.8" }, { "reproducibilityType": "Intra", "sample": "Serum", "replicates": 8, "mean": null, "standardDeviation": null, "coefficientOfVariability": "2.2" } ]

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Recovery

[ { "sample": "Serum", "range": "87 - 104 %", "average": "= 94" } ]

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What's included?

{ "values": { "1x96Tests": { "sellingSize": "1 x 96 Tests", "publicAssetCode":"ab277721-1x96Tests", "assetComponentDetails": [ { "size":"1 x 12 mL", "name":"Stop Solution", "number":"AB277721-CMP05", "productcode":"" }, { "size":"1 x 6 mL", "name":"Antibody Diluent CPR2", "number":"AB277721-CMP11", "productcode":"" }, { "size":"1 x 20 mL", "name":"10X Wash Buffer PT (ab206977)", "number":"AB277721-CMP10", "productcode":"" }, { "size":"1 x 600 µL", "name":"10X Rat IL-7 Capture Antibody", "number":"AB277721-CMP07", "productcode":"" }, { "size":"1 x 50 mL", "name":"Sample Diluent NS (ab193972)", "number":"AB277721-CMP03", "productcode":"" }, { "size":"2 x 1 Vial", "name":"Rat IL-7 Lyophilized Recombinant Protein", "number":"AB277721-CMP02", "productcode":"" }, { "size":"1 x 12 mL", "name":"TMB Development Solution", "number":"AB277721-CMP06", "productcode":"" }, { "size":"1 x 1 Unit", "name":"SimpleStep Pre-Coated 96-Well Microplate (ab206978)", "number":"AB277721-CMP04", "productcode":"" }, { "size":"1 x 1 Unit", "name":"Plate Seals", "number":"AB277721-CMP09", "productcode":"" }, { "size":"1 x 600 µL", "name":"10X Rat IL-7 Detector Antibody", "number":"AB277721-CMP08", "productcode":"" } ] } } }

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Properties and storage information

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

+4°C

Storage information

+4°C

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

The Interleukin-7 (IL7) also known as IL-7 is a cytokine with a molecular mass of approximately 20 kDa. This protein plays an important mechanical role in cell signaling. IL7 is primarily expressed in stromal epithelial cells in the thymus and bone marrow as well as in other locations such as the liver and skin. The cytokine interacts with the IL7 receptor a heterodimer consisting of the IL7Rα chain and the common gamma chain (γc). This interaction facilitates various downstream signal transductions essential for immune cell development and homeostasis.

Biological function summary

IL7 is essential for the survival and proliferation of lymphoid cells. It acts on both T cells and B cells critical components of the adaptive immune system. The protein supports the survival of naïve and memory T cells playing a significant role in T cell homeostasis. IL7 does not operate as part of a large complex but interacts directly with its receptor to exert its function. Through its influence on T and B cell proliferation the cytokine sustains lymphoid system functionality and integrity.

Pathways

IL7 plays a pivotal role in the JAK/STAT signaling pathway which is central to cellular responses to cytokines. When IL7 binds to its receptor JAK kinases activate and promote the phosphorylation of STAT5 leading to gene transcription necessary for cell survival and proliferation. Another important pathway is the PI3K/AKT signaling cascade where the cytokine supports cell survival through anti-apoptotic signals. Through these pathways IL7 is interconnected with other cytokines such as IL2 that also function through shared or similar signaling cascades contributing to immune homeostasis.

IL7 has connections with conditions like severe combined immunodeficiency (SCID) and multiple sclerosis (MS). These involve abnormal IL7 signaling or receptor mutations that affect immune cell function. In SCID mutations in the IL7 receptor result in improper T cell development leading to severe immunodeficiency. In MS altered IL7 levels may contribute to the pathogenesis by affecting the survival and activity of autoreactive T cells. Other proteins like IL2R are also involved in these conditions as they share signaling components with IL7 and their dysregulation can lead to similar immune system disturbances.

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Product protocols

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Target data

Hematopoietic cytokine that plays an essential role in the development, expansion, and survival of naive and memory T-cells and B-cells thereby regulating the number of mature lymphocytes and maintaining lymphoid homeostasis. Mechanistically, exerts its biological effects through a receptor composed of IL7RA subunit and the cytokine receptor common subunit gamma/CSF2RG. Binding to the receptor leads to activation of various kinases including JAK1 or JAK3 depending on the cell type and subsequently propagation of signals through activation of several downstream signaling pathways including the PI3K/Akt/mTOR or the JAK-STAT5.

See full target information Il7

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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Pharmaceuticals (Basel, Switzerland) 17: PubMed39204141

2024

Agave-Laurate-Bioconjugated Fructans Decrease Hyperinsulinemia and Insulin Resistance, Whilst Increasing IL-10 in Rats with Metabolic Syndrome Induced by a High-Fat Diet.

Applications

Unspecified application

Species

Unspecified reactive species

Angélica Sofía González-Garibay,Georgina Sandoval,Omar Ricardo Torres-González,Blanca Estela Bastidas-Ramírez,Iván Moisés Sánchez-Hernández,Eduardo Padilla-Camberos

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Rat IL-7 ELISA Kit

Sandwich ELISA - Rat IL-7 ELISA Kit (AB277721)

Sandwich ELISA - Rat IL-7 ELISA Kit (AB277721)

Key facts

Detection method

Sample types

Reacts with

Assay type

Results type

Sensitivity

Range

Assay time

Assay Platform

Complementary Products

Secondary Antibodies

Primary Antibodies

Assay Kits

Assay Kits

Reactivity data

Product details

Precision

Recovery

What's included?

Properties and storage information

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (1)

Product promise