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AB252353

Rat IL-9 ELISA Kit

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Rat IL-9 ELISA Kit is a single-wash 90-min Simplestep used to quantify Rat IL-9 with a sensitivity of 7.9 pg/ml. The assay uses a simple mix-wash-read protocol with just one incubation and one wash step.

- Colorimetric Sandwich ELISA - 450 nm readout : works on any standard plate reader
- Design your own immunoassay: we also offer the conjugation-ready antibody pair

View Alternative Names

Cytokine P40, HP40, Homolog of mouse T cell and mast cell growth factor 40, IL9_HUMAN, Interleukin-9, MCGF, Mast-cell growth factor, Megakaryoblast growth factor, T cell growth factor 3, T-cell growth factor P40, TCGF 3, p40 T cell and mast cell growth factor, p40 cytokine

2 Images
Sandwich ELISA - Rat IL-9 ELISA Kit (AB252353)
  • sELISA

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Sandwich ELISA - Rat IL-9 ELISA Kit (AB252353)

Example of rat IL-9 standard curve in Sample Diluent NS.

The IL-9 standard curve was prepared as described in Section 10. Raw data values are shown in the table. Background-subtracted data values (mean +/- SD) are graphed.

Sandwich ELISA - Rat IL-9 ELISA Kit (AB252353)
  • sELISA

Unknown

Sandwich ELISA - Rat IL-9 ELISA Kit (AB252353)

Interpolated concentrations of native IL-9 in rat tissue culture supernatant samples.

Rat spleen tissue was cultured in the presence of 10 ng/mL PMA and 10 ng/mL rat IL-1a for 4 days (PMA + IL-1a) or 50 ng/mL PMA and 500 ng/mL calcium ionomycin for 3 days (PMA + calcium ionomycin). Spleen tissue was cultured without treatment for 2 days (unstimulated). The concentrations of IL-9 were measured in duplicates, interpolated from the IL-9 standard curves and corrected for sample dilution. Undiluted samples are as follows : PMA + IL-1a 100%, PMA + calcium ionomycin 100%, and unstimulated 100%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean IL-9 concentration was determined to be 91.51 pg/mL in neat PMA + IL-1a stimulated spleen tissue supernatant, 126.3 pg/mL in neat PMA + calcium ionomycin stimulated spleen tissue supernatant, and 19.64 pg/mL in neat unstimulated spleen tissue supernatant.

Key facts

Detection method

Colorimetric

Sample types

Cell culture media, Heparin Plasma, Citrate plasma, Serum, Tissue Culture Media, EDTA Plasma

Reacts with

Rat

Assay type

Sandwich (quantitative)

Sensitivity

= 7.9 pg/mL

Range

23.44 - 1500 pg/mL

Assay time

1h 30m

Assay Platform

Pre-coated microplate (12 x 8 well strips)

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Product details

Rat IL-9 ELISA Kit (ab252353) is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of IL-9 protein in cit plasma, edta plasma, hep plasma, serum, tissue culture media, and cell culture media. It uses our proprietary SimpleStep ELISA® technology. Quantitate Rat IL-9 with 7.9 pg/ml sensitivity.

SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:

- Single-wash protocol reduces assay time to 90 minutes or less
- High sensitivity, specificity and reproducibility from superior antibodies
- Fully validated in biological samples
- 96-wells plate breakable into 12 x 8 wells strips

A 384-well SimpleStep ELISA® microplate (ab203359) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.

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Precision

[ { "reproducibilityType": "Inter", "sample": "Serum", "replicates": 3, "mean": null, "standardDeviation": null, "coefficientOfVariability": "6.8" }, { "reproducibilityType": "Intra", "sample": "Serum", "replicates": 8, "mean": null, "standardDeviation": null, "coefficientOfVariability": "3.2" } ]
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Recovery

[ { "sample": "Serum", "range": "78 - 79 %", "average": "= 79" }, { "sample": "EDTA Plasma", "range": "89 - 94 %", "average": "= 91" }, { "sample": "Tissue Culture Media", "range": "99 - 107 %", "average": "= 103" }, { "sample": "Cell culture media", "range": "96 - 101 %", "average": "= 98" }, { "sample": "Heparin Plasma", "range": "75 - 77 %", "average": "= 76" }, { "sample": "Citrate plasma", "range": "83 - 85 %", "average": "= 84" } ]
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What's included?

{ "values": { "1x96Tests": { "sellingSize": "1 x 96 Tests", "publicAssetCode":"ab252353-1x96Tests", "assetComponentDetails": [ { "size":"1 x 12 mL", "name":"Stop Solution", "number":"AB252353-CMP07", "productcode":"" }, { "size":"1 x 20 mL", "name":"10X Wash Buffer PT (ab206977)", "number":"AB252353-CMP10", "productcode":"" }, { "size":"1 x 50 mL", "name":"Sample Diluent NS (ab193972)", "number":"AB252353-CMP05", "productcode":"" }, { "size":"1 x 12 mL", "name":"TMB Development Solution", "number":"AB252353-CMP08", "productcode":"" }, { "size":"1 x 1 Unit", "name":"SimpleStep Pre-Coated 96-Well Microplate (ab206978)", "number":"AB252353-CMP06", "productcode":"" }, { "size":"1 x 1 Unit", "name":"Plate Seals", "number":"AB252353-CMP09", "productcode":"" }, { "size":"1 x 6 mL", "name":"Antibody Diluent 5BR", "number":"AB252353-CMP01", "productcode":"" }, { "size":"1 x 600 µL", "name":"10X Rat IL-9 Detector Antibody", "number":"AB252353-CMP03", "productcode":"" }, { "size":"2 x 1 Vial", "name":"Rat IL-9 Lyophilized Recombinant Protein", "number":"AB252353-CMP04", "productcode":"" }, { "size":"1 x 600 µL", "name":"10X Rat IL-9 Capture Antibody", "number":"AB252353-CMP02", "productcode":"" } ] } } }
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Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
+4°C
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Interleukin-9 (IL-9) sometimes known as a T-cell growth factor is a cytokine with a molecular mass of approximately 14-19 kDa. It is primarily expressed by T helper cells particularly Th2 and Th9 cells. IL-9 comes from the IL-9 gene and plays a significant role in the immune system by affecting various cell types such as mast cells B cells and epithelial cells. Researchers first identified it in the late 1980s and it has continued to be an area of interest due to its diverse functions.
Biological function summary

IL-9 influences immune responses and is important for various processes including cell proliferation and survival. IL-9 activates mast cells which play role in allergy response. It also affects the growth and differentiation of T cells and enhances the function of dendritic cells. IL-9 operates individually and not as part of a larger complex but works closely with its receptor IL-9R to initiate downstream signaling events.

Pathways

Cytokine signaling pathways and JAK-STAT signaling pathways integrate IL-9's function in the immune system. IL-9 activates the JAK-STAT pathway particularly involving proteins like JAK1 and STATs to relay signals that promote immune cell growth and survival. This cytokine also interlinks with other cytokines such as IL-4 and IL-13 playing role in immune regulation and inflammatory responses.

IL-9 shows strong correlation with asthma and certain types of cancers particularly Hodgkin's lymphoma. In asthma overproduction of IL-9 can lead to increased mucus production and airway hyperresponsiveness. This relationship makes it a potential target for blocking therapies in asthma treatment. Higher levels of IL-9 correlate with poorer prognosis in some cancers indicating its potential role in tumor growth and immune evasion possibly interacting with IL-6 in such conditions.
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Product protocols

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Target data

See full target information Il9
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websiteProtocolBooklet
en
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