Rat Kallikrein 10 ELISA Kit is a single-wash 90-min Simplestep used to quantify Rat Kallikrein 10 with a sensitivity of 2.35 pg/ml. The assay uses a simple mix-wash-read protocol with just one incubation and one wash step.
- Colorimetric Sandwich ELISA - 450 nm readout : works on any standard plate reader
- Design your own immunoassay: we also offer the conjugation-ready antibody pair
Images
Key facts
- Detection method
- Colorimetric
- Sample types
- Cell culture media, Heparin Plasma, Serum
- Assay type
- Sandwich (quantitative)
- Reactive species
- Rat
- Range
- 10.16 - 650 pg/mL
- Assay time
- 1h 30m
- Sensitivity
- = 2.35 pg/mL
Reactivity data
| Application | Reactivity | Dilution info | Notes |
|---|---|---|---|
Application sELISA | Reactivity Reacts | Dilution info - | Notes - |
Target data
Function
Glandular kallikreins cleave Met-Lys and Arg-Ser bonds in kininogen to release Lys-bradykinin. This protein may be involved in the regulation of renal function.
Alternative names
Klk-10, Klk10, Glandular kallikrein-10, K10, rGK-10, Endopeptidase K, Proteinase B, T-kininogenase, Tissue kallikrein
What's included?
Recommended products
Rat Kallikrein 10 ELISA Kit is a single-wash 90-min Simplestep used to quantify Rat Kallikrein 10 with a sensitivity of 2.35 pg/ml. The assay uses a simple mix-wash-read protocol with just one incubation and one wash step.
- Colorimetric Sandwich ELISA - 450 nm readout : works on any standard plate reader
- Design your own immunoassay: we also offer the conjugation-ready antibody pair
Key facts
- Detection method
- Colorimetric
- Sample types
- Cell culture media, Heparin Plasma, Serum
- Assay type
- Sandwich (quantitative)
- Reactive species
- Rat
- Range
- 10.16 - 650 pg/mL
- Assay time
- 1h 30m
- Assay Platform
- Pre-coated microplate (12 x 8 well strips)
- Sensitivity
- = 2.35 pg/mL
Precision
Intra assay
| Sample | n | mean | SD | C.V. |
|---|---|---|---|---|
Sample Serum | n 8 | mean - | SD - | C.V. 2.1 |
Recovery
Sample specific recovery
| Sample type | Average % | Range |
|---|---|---|
Sample type Serum | Average % = 91 | Range |
Sample type Cell culture media | Average % = 96 | Range |
Sample type Heparin Plasma | Average % = 94 | Range |
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- +4°C
- Storage information
- +4°C
Notes
Rat Kallikrein 10 ELISA kit (Human Neurofilament H ELISA Kit (200 kDa Neurofilament protein) ab272199) is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of Rat Kallikrein 10 protein in rat serum, plasma, and cell culture media samples. It uses our proprietary SimpleStep ELISA® technology. Quantitate Rat Kallikrein 10 with 2.35 pg/mL sensitivity.
SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:
-Single-wash protocol reduces assay time to 90 minutes or less
-High sensitivity, specificity and reproducibility from superior antibodies
-Fully validated in biological samples
-96-wells plate breakable into 12 x 8 wells strips
A 384-well SimpleStep ELISA® microplate (Pre-coated 384 well Microplate SimpleStep ELISA® ab203359) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
Kallikrein 10 also known as KLK10 or serine protease 20 is a protein known for its enzymatic activity. It has a mass of approximately 30 kDa and expresses highly in tissues like the pancreas kidney and hormonal dependent tissues such as the prostate and breast. KLK10 involves in the degradation of extracellular matrix components functioning as a serine protease. Its expression in hormone-responsive tissues indicates a potential role in processes like tissue remodeling and regulation.
- Biological function summary
Kallikrein 10 is part of a larger family of serine proteases involved in various physiological processes. These proteases participate in tissue homeostasis and in some instances immune response. KLK10 expression links to cell proliferation and survival potentially influencing cancer progression. The network of serine proteases forms a proteolytic cascade where multiple proteases sequentially activate amplifying the biological responses.
- Pathways
Kallikrein 10 plays a critical role in the proteolytic cascade responsible for regulating homeostatic processes. It participates notably in pathways such as the kallikrein-kinin system and the regulation of tumor microenvironment. KLK10 interacts with other kallikreins like KLK5 and KLK6 mediating their activation and functioning in tissue remodeling and repair processes.
- Associated diseases and disorders
Kallikrein 10 has a significant association with cancers and inflammatory conditions. Altered KLK10 expression shows correlation with prostate cancer and breast cancer where it might serve as a biomarker for diagnosis or prognosis. KLK10's involvement with proteins like KLK3 (PSA) in prostate cancer reflects its potential utility in therapeutic strategies especially concerning hormone-dependent tumors.
Product promise
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
2 product images
Sandwich ELISA - Rat Kallikrein 10 ELISA Kit (ab284619)
Example of rat Kallikrein 10 standard curve in Sample Diluent NS.
Example rat Kallikrein 10 standard curve. Background-subtracted data values (mean =/- SD) are graphed.
Sandwich ELISA - Rat Kallikrein 10 ELISA Kit (ab284619)
Interpolated concentrations of rat Kallikrein 10 in serum and plasma (heparin).
The endogenous concentration of Kallikrein 10 was measured in duplicate at different sample concentrations. Undiluted samples are 25% serum and plasma (heparin). The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). Sample dilutions are made in Sample Diluent NS.
Downloads
Product protocols
- Visit the general protocols
- Visit troubleshooting
Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.
For licensing inquiries, please contact partnerships@abcam.com