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AB284619

Rat Kallikrein 10 ELISA Kit

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Rat Kallikrein 10 ELISA Kit is a single-wash 90-min Simplestep used to quantify Rat Kallikrein 10 with a sensitivity of 2.35 pg/ml. The assay uses a simple mix-wash-read protocol with just one incubation and one wash step.

- Colorimetric Sandwich ELISA - 450 nm readout : works on any standard plate reader
- Design your own immunoassay: we also offer the conjugation-ready antibody pair

View Alternative Names

Klk-10, Klk10, Glandular kallikrein-10, K10, rGK-10, Endopeptidase K, Proteinase B, T-kininogenase, Tissue kallikrein

2 Images
Sandwich ELISA - Rat Kallikrein 10 ELISA Kit (AB284619)
  • sELISA

Supplier Data

Sandwich ELISA - Rat Kallikrein 10 ELISA Kit (AB284619)

Interpolated concentrations of rat Kallikrein 10 in serum and plasma (heparin).

The endogenous concentration of Kallikrein 10 was measured in duplicate at different sample concentrations. Undiluted samples are 25% serum and plasma (heparin). The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). Sample dilutions are made in Sample Diluent NS.

Sandwich ELISA - Rat Kallikrein 10 ELISA Kit (AB284619)
  • sELISA

Supplier Data

Sandwich ELISA - Rat Kallikrein 10 ELISA Kit (AB284619)

Example of rat Kallikrein 10 standard curve in Sample Diluent NS.

Example rat Kallikrein 10 standard curve. Background-subtracted data values (mean =/- SD) are graphed.

Key facts

Detection method

Colorimetric

Sample types

Cell culture media, Heparin Plasma, Serum

Reacts with

Rat

Assay type

Sandwich

Results type

Quantitative

Sensitivity

= 2.35 pg/mL

Range

10.16 - 650 pg/mL

Assay time

1h 30m

Assay Platform

Pre-coated microplate (12 x 8 well strips)

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Product details

Rat Kallikrein 10 ELISA kit (ab272199) is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of Rat Kallikrein 10 protein in rat serum, plasma, and cell culture media samples. It uses our proprietary SimpleStep ELISA® technology. Quantitate Rat Kallikrein 10 with 2.35 pg/mL sensitivity.

SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:

-Single-wash protocol reduces assay time to 90 minutes or less
-High sensitivity, specificity and reproducibility from superior antibodies
-Fully validated in biological samples
-96-wells plate breakable into 12 x 8 wells strips

A 384-well SimpleStep ELISA® microplate (ab203359) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.

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Precision

[ { "reproducibilityType": "Intra", "sample": "Serum", "replicates": 8, "mean": null, "standardDeviation": null, "coefficientOfVariability": "2.1" } ]
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Recovery

[ { "sample": "Serum", "range": null, "average": "= 91" }, { "sample": "Cell culture media", "range": null, "average": "= 96" }, { "sample": "Heparin Plasma", "range": null, "average": "= 94" } ]
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What's included?

{ "values": { "1x96Tests": { "sellingSize": "1 x 96 Tests", "publicAssetCode":"ab284619-1x96Tests", "assetComponentDetails": [ { "size":"1 x 12 mL", "name":"Stop Solution", "number":"AB284619-CMP05", "productcode":"" }, { "size":"1 x 1 Unit", "name":"SimpleStep Pre-Coated 96-Well Microplate (ab206978)", "number":"AB284619-CMP04", "productcode":"" }, { "size":"1 x 12 mL", "name":"TMB Development Solution", "number":"AB284619-CMP06", "productcode":"" }, { "size":"1 x 6 mL", "name":"Antibody Diluent 5BR", "number":"AB284619-CMP02", "productcode":"" }, { "size":"1 x 20 mL", "name":"10X Wash Buffer PT (ab206977)", "number":"AB284619-CMP10", "productcode":"" }, { "size":"1 x 600 µL", "name":"10X Rat Kallikrein 10 Detector Antibody", "number":"AB284619-CMP08", "productcode":"" }, { "size":"1 x 1 Unit", "name":"Plate Seals", "number":"AB284619-CMP09", "productcode":"" }, { "size":"1 x 12 mL", "name":"Sample Diluent NS (ab193972)", "number":"AB284619-CMP11", "productcode":"" }, { "size":"1 x 600 µL", "name":"10X Rat Kallikrein 10 Capture Antibody", "number":"AB284619-CMP07", "productcode":"" }, { "size":"2 x 1 Vial", "name":"Rat Kallikrein 10 Lyophilized Recombinant Protein", "number":"AB284619-CMP03", "productcode":"" } ] } } }
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Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
+4°C
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Kallikrein 10 also known as KLK10 or serine protease 20 is a protein known for its enzymatic activity. It has a mass of approximately 30 kDa and expresses highly in tissues like the pancreas kidney and hormonal dependent tissues such as the prostate and breast. KLK10 involves in the degradation of extracellular matrix components functioning as a serine protease. Its expression in hormone-responsive tissues indicates a potential role in processes like tissue remodeling and regulation.
Biological function summary

Kallikrein 10 is part of a larger family of serine proteases involved in various physiological processes. These proteases participate in tissue homeostasis and in some instances immune response. KLK10 expression links to cell proliferation and survival potentially influencing cancer progression. The network of serine proteases forms a proteolytic cascade where multiple proteases sequentially activate amplifying the biological responses.

Pathways

Kallikrein 10 plays a critical role in the proteolytic cascade responsible for regulating homeostatic processes. It participates notably in pathways such as the kallikrein-kinin system and the regulation of tumor microenvironment. KLK10 interacts with other kallikreins like KLK5 and KLK6 mediating their activation and functioning in tissue remodeling and repair processes.

Kallikrein 10 has a significant association with cancers and inflammatory conditions. Altered KLK10 expression shows correlation with prostate cancer and breast cancer where it might serve as a biomarker for diagnosis or prognosis. KLK10's involvement with proteins like KLK3 (PSA) in prostate cancer reflects its potential utility in therapeutic strategies especially concerning hormone-dependent tumors.
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Product protocols

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Target data

Glandular kallikreins cleave Met-Lys and Arg-Ser bonds in kininogen to release Lys-bradykinin. This protein may be involved in the regulation of renal function.
See full target information Klk10
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websiteProtocolBooklet
en
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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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