AB119597

Rat KIM-1 TIM-1 ELISA Kit

Name: Rat KIM-1 TIM-1 ELISA Kit (ab119597) | Abcam
Brand: Abcam Limited
SKU: AB119597

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(17 Publications)

Rat KIM-1 TIM-1 ELISA Kit is a Sandwich (quantitative) ELISA for the measurement of Rat KIM-1 TIM-1 in Rat in Biofluids, Cell Culture Media samples.

View Alternative Names

CD365, Kim1, Havcr1, Hepatitis A virus cellular receptor 1 homolog, HAVcr-1, Kidney injury molecule 1, T cell immunoglobulin and mucin domain-containing protein 1, KIM-1, TIMD-1

1 Images

Sandwich ELISA - Rat KIM-1 TIM-1 ELISA Kit (AB119597)

sELISA

Supplier Data

Sandwich ELISA - Rat KIM-1 TIM-1 ELISA Kit (AB119597)

Representative Standard Curve using ab119597.

Key facts

Detection method

Colorimetric

Sample types

Urine, Plasma, Cell culture supernatant, Serum

Reacts with

Rat

Assay type

Sandwich (quantitative)

Sensitivity

< 2 pg/mL

Range

31.2 - 2000 pg/mL

Assay Platform

Microplate

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }

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Product details

Abcam's rat KIM-1 (TIM-1) in vitro ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for the accurate quantitative measurement of rat KIM-1 (TIM-1) in cell culture supernatants, tissue homogenates, serum, plasma (heparin, EDTA) and urine.

A KIM-1 (TIM-1) specific mouse monoclonal antibody has been precoated onto 96-well plates. Standards and test samples are added to the wells and incubated. A biotinylated detection polyclonal antibody from goat specific for KIM-1 (TIM-1) is then added followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex is added and unbound conjugates are washed away with PBS or TBS buffer. TMB is then used to visualize the HRP enzymatic reaction. TMB is catalyzed by HRP to produce a blue color product that changes into yellow after adding acidic stop solution. The density of yellow coloration is directly proportional to the rat KIM-1 (TIM-1) amount of sample captured in plate.

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What's included?

{ "values": { "1x96Tests": { "sellingSize": "1 x 96 Tests", "publicAssetCode":"ab119597-1x96Tests", "assetComponentDetails": [ { "size":"1 x 30 mL", "name":"Sample Diluent Buffer", "number":"AB119597-CMP04", "productcode":"" }, { "size":"1 x 100 µL", "name":"Biotinylated anti-rat KIM-1 antibody", "number":"AB119597-CMP09", "productcode":"" }, { "size":"1 x 1 Unit", "name":"Anti-rat KIM-1 antibody Microplate (12 x 8 wells)", "number":"AB119597-CMP02", "productcode":"" }, { "size":"1 x 10 mL", "name":"TMB Color Developing Agent", "number":"AB119597-CMP06", "productcode":"" }, { "size":"1 x 100 µL", "name":"Avidin-Biotin-Peroxidase Complex (ABC)", "number":"AB119597-CMP10", "productcode":"" }, { "size":"4 x 1 Unit", "name":"Plate Seal", "number":"AB119597-CMP01", "productcode":"" }, { "size":"1 x 12 mL", "name":"Antibody Diluent Buffer", "number":"AB119597-CMP08", "productcode":"" }, { "size":"1 x 12 mL", "name":"ABC Diluent Buffer", "number":"AB119597-CMP05", "productcode":"" }, { "size":"1 x 10 mL", "name":"TMB Stop Solution", "number":"AB119597-CMP07", "productcode":"" }, { "size":"2 x 10 ng", "name":"Lyophilized recombinant rat KIM-1 standard", "number":"AB119597-CMP03", "productcode":"" }, { "size":"1 x 20 mL", "name":"Wash Buffer (25X)", "number":"AB119597-CMP11", "productcode":"" } ] } } }

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Properties and storage information

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

-20°C

Appropriate long-term storage conditions

-20°C

Storage information

-20°C

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

TIM 1 also known as T-cell immunoglobulin and mucin domain 1 is a cell surface protein with a molecular mass of approximately 70 to 80 kDa. It is expressed predominantly in activated T cells and some epithelial cells. TIM 1 plays an important role in immune regulation and can be found at the surface of cells in various tissues including the liver and kidney. This protein can interact with other components which can influence immune response in the host.

Biological function summary

The TIM 1 protein functions as a receptor involved in T cell activity modulation and innate immune response regulation. It is known to bind phosphatidylserine on apoptotic cells which fosters communication between dying cells and phagocytes. TIM 1 does not typically exist as part of a larger complex but its interactions with other molecules is important for its role in the immune system. The protein’s activity influences cytokine production and this can affect inflammation processes.

Pathways

TIM 1 is involved in key immune response pathways like the signaling pathways of T cells and the broader adaptive immune response. This protein has been linked with the enhancement of Th2 cytokine production impacting allergic response signaling. It interacts with related proteins like TIM 3 and TIM 4 which together contribute to the fine-tuning of immune responses. Understanding TIM 1 within these pathways helps in grasping its regulatory potential in T-cell mediated immunity.

TIM 1 is implicated in conditions such as asthma and systemic lupus erythematosus (SLE). In asthma improperly regulated Th2 immune responses involve TIM 1 which can exacerbate allergic inflammations. This protein also plays a role in the pathogenesis of SLE a systemic autoimmune disease where TIM 1's regulation of immune tolerance and inflammation is critical. TIM 1’s involvement with other proteins like IL-4 and IL-13 in these disorders further highlights its significance in immune dysregulation processes.

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Product protocols

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Target data

Phosphatidylserine receptor that plays an important functional role in regulatory B-cells homeostasis including generation, expansion and suppressor functions (By similarity). As P-selectin/SELPLG ligand, plays a specialized role in activated but not naive T-cell trafficking during inflammatory responses. Controls thereby T-cell accumulation in the inflamed central nervous system (CNS) and the induction of autoimmune disease (By similarity). Regulates also expression of various anti-inflammatory cytokines and co-inhibitory ligands including IL10. Acts as a regulator of T-cell proliferation (By similarity). May play a role in kidney injury and repair (By similarity).

See full target information Havcr1

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Publications (17)

Recent publications for all applications. Explore the full list and refine your search

GeroScience 46:6435-6458 PubMed38976131

2024

Integrated renal and sympathetic mechanisms underlying the development of sex- and age-dependent hypertension and the salt sensitivity of blood pressure.

Applications

Unspecified application

Species

Unspecified reactive species

Alissa A Frame,Kayla M Nist,Kiyoung Kim,Franco Puleo,Jesse D Moreira,Hailey Swaldi,James McKenna,Richard D Wainford

PloS one 18:e0283605 PubMed37022999

2023

Effects of Mexican Ganoderma lucidum extracts on liver, kidney, and the gut microbiota of Wistar rats: A repeated dose oral toxicity study.

Applications

Unspecified application

Species

Unspecified reactive species

María E Meneses,Daniel Martínez-Carrera,Laura González-Ibáñez,Nimbe Torres,Mónica Sánchez-Tapia,Claudia C Márquez-Mota,Gilmar Rendón,Vladimir Mitzi,Alfredo Morales,Isaac Tello-Salgado,Armando R Tovar

Current issues in molecular biology 45:1183-1196 PubMed36826023

2023

Myrrh Essential Oil Mitigates Renal Ischemia/Reperfusion-Induced Injury.

Applications

Unspecified application

Species

Unspecified reactive species

Nancy S Younis

Antioxidants (Basel, Switzerland) 11: PubMed36009287

2022

Renal Ischemia/Reperfusion Mitigation via Geraniol: The Role of Nrf-2/HO-1/NQO-1 and TLR2,4/MYD88/NFκB Pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Maged E Mohamed,Mohammad A Elmorsy,Nancy S Younis

Journal of leukocyte biology 112:1065-1077 PubMed35774015

2022

Ginsenoside Rg1 ameliorates sepsis-induced acute kidney injury by inhibiting ferroptosis in renal tubular epithelial cells.

Applications

Unspecified application

Species

Unspecified reactive species

Jun Guo,Rong Wang,Fei Min

Antioxidants (Basel, Switzerland) 11: PubMed35326185

2022

The Protective Effect of Anethole against Renal Ischemia/Reperfusion: The Role of the TLR2,4/MYD88/NFκB Pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Maged Elsayed Mohamed,Mahmoud Kandeel,Hany M Abd El-Lateef,Hossam S El-Beltagi,Nancy S Younis

Frontiers in pharmacology 12:801094 PubMed35222012

2022

Ameliorates Renal Hypertensive Injury and Fibrosis Through the Regulation of SIRT1-Mediated Autophagy.

Applications

Unspecified application

Species

Unspecified reactive species

Yuzi Cai,Zhendong Feng,Qi Jia,Jing Guo,Pingna Zhang,Qihan Zhao,Yao Xian Wang,Yu Ning Liu,Wei Jing Liu

Journal of the American Heart Association 10:e020088 PubMed33682436

2021

Long-Term Dipeptidyl Peptidase 4 Inhibition Worsens Hypertension and Renal and Cardiac Abnormalities in Obese Spontaneously Hypertensive Heart Failure Rats.

Applications

Unspecified application

Species

Unspecified reactive species

Edwin K Jackson,Zaichuan Mi,Delbert G Gillespie,Dongmei Cheng,Stevan P Tofovic

Molecular medicine reports 22:2655-2664 PubMed32945382

2020

Tetramethylpyrazine alleviates acute kidney injury by inhibiting NLRP3/HIF‑1α and apoptosis.

Applications

Unspecified application

Species

Unspecified reactive species

Wangnan Sun,Aiqun Li,Zhiqiang Wang,Xuhong Sun,Menghua Dong,Fu Qi,Lin Wang,Yueheng Zhang,Pengchao Du

Anais da Academia Brasileira de Ciencias 92:e20191237 PubMed32638872

2020

Involvement of redox status and the nuclear-related factor 2 in protecting against cadmium-induced renal injury with Sana Makki (Cassia senna L.) pre-treatment in male rats.

Applications

Unspecified application

Species

Unspecified reactive species

Gadah Albasher,Tarfa Albrahim,Nada Aljarba,Raedah I Alharbi,Nouf Alsultan,Jawaher Alsaiari,Humaira Rizwana

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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Rat KIM-1 TIM-1 ELISA Kit

Sandwich ELISA - Rat KIM-1 TIM-1 ELISA Kit (AB119597)

Key facts

Detection method

Sample types

Reacts with

Assay type

Sensitivity

Range

Assay Platform

Reactivity data

Product details

What's included?

Properties and storage information

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (17)

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