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AB157742

Rat Kininogen ELISA Kit (KNG) ELISA Kit

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(1 Publication)

Rat Kininogen ELISA Kit (KNG) ELISA Kit is a Sandwich ELISA for the measurement of Rat Kininogen (KNG) in Rat in Biofluids samples.

View Alternative Names

Kng, Kng1, Kininogen-1

1 Images
Sandwich ELISA - Rat Kininogen ELISA Kit (KNG) ELISA Kit (AB157742)
  • sELISA

Supplier Data

Sandwich ELISA - Rat Kininogen ELISA Kit (KNG) ELISA Kit (AB157742)

Representative standard curve using ab157742 Kininogen Rat ELISA Kit

Key facts

Detection method

Colorimetric

Sample types

Plasma, Serum

Reacts with

Rat

Assay type

Sandwich

Results type

Quantitative

Sensitivity

= 4.9707 ng/mL

Range

6.25 - 400 ng/mL

Assay Platform

Microplate

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Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488)

Immunocytochemistry/ Immunofluorescence - Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (AB150077)

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Primary Antibodies

AB124737

Anti-Kininogen 1 antibody [EPR6097]

RabMAb|Recombinant

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Kininogen 1 antibody [EPR6097] (AB124737)

  • 0
  • 0
Primary Antibodies

AB7817

Anti-alpha smooth muscle Actin antibody [1A4]

KO Validated

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-alpha smooth muscle Actin antibody [1A4] (AB7817)

  • 5
  • 105
Primary Antibodies

AB192890

Anti-LC3B antibody [EPR18709] - Autophagosome Marker

BOND RX™ Validated|RabMAb|Recombinant|KO Validated|Lab Essentials|20ul selling size

Western blot - Anti-LC3B antibody [EPR18709] - Autophagosome Marker (AB192890)

  • 5
  • 19
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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Product details

Abcam's Kininogen (KNG) Rat ELISA Kit is a two-site enzyme-linked immunosorbent assay (ELISA) for the quantitative measurement of Kininogen levels in rat serum and plasma.

In this assay the Kininogen present in samples reacts with the anti-Kininogen antibodies which have been adsorbed to the surface of polystyrene microtitre wells. After the removal of unbound proteins by washing, anti-Kininogen antibodies conjugated with horseradish peroxidase (HRP), are added. These enzyme-labeled antibodies form complexes with the previously bound Kininogen. Following another washing step, the enzyme bound to the immunosorbent is assayed by the addition of a chromo­genic substrate, 3,3',5,5'-tetramethylbenzidine (TMB). The quantity of bound enzyme varies directly with the concentration of Kininogen in the sample tested; thus, the absorbance, at 450 nm, is a measure of the concentration of Kininogen in the test sample. The quantity of Kininogen in the test sample can be interpolated from the standard curve constructed from the standards, and corrected for sample dilution.

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Precision

[ { "reproducibilityType": "Inter", "sample": "Overall", "replicates": 0, "mean": null, "standardDeviation": null, "coefficientOfVariability": "< 10" }, { "reproducibilityType": "Intra", "sample": "Overall", "replicates": 0, "mean": null, "standardDeviation": null, "coefficientOfVariability": "< 10" } ]
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Recovery

[ { "sample": "Serum", "range": null, "average": "> 85" } ]
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What's included?

{ "values": { "1x96Tests": { "sellingSize": "1 x 96 Tests", "publicAssetCode":"ab157742-1x96Tests", "assetComponentDetails": [ { "size":"1 x 50 mL", "name":"5X Diluent Concentrate", "number":"AB157742-CMP07", "productcode":"" }, { "size":"1 x 50 mL", "name":"20X Wash Buffer Concentrate", "number":"AB157742-CMP06", "productcode":"" }, { "size":"1 x 150 µL", "name":"100X HRP-conjugated anti-rat Kininogen antibody", "number":"AB157742-CMP05", "productcode":"" }, { "size":"1 x 12 mL", "name":"Chromogen Substrate Solution", "number":"AB157742-CMP04", "productcode":"" }, { "size":"1 x 12 mL", "name":"Stop Solution", "number":"AB157742-CMP03", "productcode":"" }, { "size":"1 x 1 Unit", "name":"Rat Kininogen ELISA Microplate", "number":"AB157742-CMP02", "productcode":"" }, { "size":"1 x 1 Vial", "name":"Rat Kininogen Calibrator (Lyophilized)", "number":"AB157742-CMP01", "productcode":"" } ] } } }
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Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
Multi
Storage information
Please refer to protocols
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

The kininogen is a protein involved in various biological processes and also known as high molecular weight kininogen (HMWK). This protein which has a mass of approximately 120 kDa circulates in the plasma. Kininogen is synthesized by the liver and endothelial cells where it exists as HMWK. It serves as a precursor to kinins like bradykinin which are important in regulating blood pressure and inflammation.
Biological function summary

Kininogen participates in the kallikrein-kinin system. It plays an important role in blood coagulation and the inflammatory response. HMWK is not part of a complex but interacts with factors such as prekallikrein and Factor XII. By serving as a co-factor HMWK facilitates the activation of these molecules which progresses the coagulation cascade and inflammatory pathways.

Pathways

The kininogen functions within the kallikrein-kinin and coagulation systems. It interacts closely with enzymes like kallikrein which cleaves kininogen to release bradykinin assisting in vasodilation and increasing vascular permeability. Additionally HMWK is tethered to the intrinsic pathway of coagulation by activation of Factor XII which links it to proteins involved in blood clot formation.

The kininogen is associated with hereditary angioedema and bradykinin-mediated edema. Abnormalities in its function can lead to excessive bradykinin release. This condition involves excessive swelling due to alterations in vascular permeability. Moreover the protein is linked to chronic inflammatory diseases through its connection with the bradykinin receptor and related peptides.
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Product protocols

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Target data

Kininogens are inhibitors of thiol proteases. HMW-kininogen plays an important role in blood coagulation by helping to position optimally prekallikrein and factor XI next to factor XII; HMW-kininogen inhibits the thrombin- and plasmin-induced aggregation of thrombocytes. LMW-kininogen inhibits the aggregation of thrombocytes. LMW-kininogen is in contrast to HMW-kininogen not involved in blood clotting.. Bradykinin. The active peptide bradykinin is a potent vasodilatator that is released from HMW-kininogen shows a variety of physiological effects : (A) influence in smooth muscle contraction, (B) induction of hypotension, (C) natriuresis and diuresis, (D) decrease in blood glucose level, (E) it is a mediator of inflammation and causes (E1) increase in vascular permeability, (E2) stimulation of nociceptors (4E3) release of other mediators of inflammation (e.g. prostaglandins), (F) it has a cardioprotective effect (directly via bradykinin action, indirectly via endothelium-derived relaxing factor action).
See full target information Kng1
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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Frontiers in neurology 10:37 PubMed30804871

2019

Kininogen Level in the Cerebrospinal Fluid May Be a Potential Biomarker for Predicting Epileptogenesis.

Applications

Unspecified application

Species

Unspecified reactive species

Jing Zou,Xinxin Wang,Ligang Huang,Juan Liu,Yingying Kong,Shengtian Li,Qinchi Lu
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websiteProtocolBooklet
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