Rat L-Selectin ELISA Kit
- Recombinant
- SimpleStep
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Rat L-Selectin ELISA Kit is a single-wash 90-min Simplestep used to quantify Rat L-Selectin with a sensitivity of 0.77 ng/mL. The assay uses a simple mix-wash-read protocol with just one incubation and one wash step.
- Colorimetric Sandwich ELISA - 450 nm readout : works on any standard plate reader
- Design your own immunoassay: we also offer the conjugation-ready antibody pair
View Alternative Names
CD62L, Lnhr, Ly-22, Sell, L-selectin, CD62 antigen-like family member L, Leukocyte adhesion molecule 1, Leukocyte-endothelial cell adhesion molecule 1, Lymph node homing receptor, Lymphocyte antigen 22, Lymphocyte surface MEL-14 antigen, LAM-1, LECAM1
- sELISA
Supplier Data
Sandwich ELISA - Rat L-Selectin ELISA Kit (AB263876)
Interpolated concentrations of native L-Selectin in serum, rat plasma (EDTA), and plasma (citrate) samples.
The concentrations of L-Selectin were measured in duplicates, interpolated from the L-Selectin standard curves and corrected for sample dilution. Undiluted samples are as follows : serum 50%, plasma (EDTA) 100%, and plasma (citrate) 100%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean L-Selectin concentration was determined to be 41.8 ng/mL in serum, 30 ng/mL in plasma (EDTA), and 25.1 ng/mL in plasma (citrate).
- sELISA
Supplier Data
Sandwich ELISA - Rat L-Selectin ELISA Kit (AB263876)
Interpolated concentrations of native L-Selectin in rat spleen cell supernatant samples.
The concentrations of L-Selectin were measured in duplicates, interpolated from the L-Selectin standard curves and corrected for sample dilution. Undiluted samples are as follows : spleen cell supernatant 100%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean L-Selectin concentration was determined to be 44.1 ng/mL in spleen cell supernatant.
- sELISA
Supplier Data
Sandwich ELISA - Rat L-Selectin ELISA Kit (AB263876)
Example of rat L-Selectin standard curve in Sample Diluent NS.
The L-Selectin standard curve was prepared as described in Section 10. Raw data values are shown in the table. Background-subtracted data values (mean +/- SD) are graphed.
Reactivity data
Product details
Rat L-Selectin ELISA Kit (ab263876) is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of L-Selectin protein in cell culture supernatant, cit plasma, edta plasma, and serum. It uses our proprietary SimpleStep ELISA® technology. Quantitate Rat L-Selectin with 0.77 ng/ml sensitivity.
SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:
- Single-wash protocol reduces assay time to 90 minutes or less
- High sensitivity, specificity and reproducibility from superior antibodies
- Fully validated in biological samples
- 96-wells plate breakable into 12 x 8 wells strips
A 384-well SimpleStep ELISA® microplate (ab203359) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.
L-Selectin, also referred to as CD62L and Leukocyte adhesion molecule 1, is a calcium-dependent lectin encoded by the gene SELL. L-Selectin binds to glycoproteins to mediate cell adhesion on neighboring cells. In peripheral lymph nodes, L-selectin mediates the adherence of lymphocytes to endothelial cells with high endothelial venules. L-Selectin is composed of several domains, one homologous to lectins, an epidermal growth factor domain, and two to repeat units found In C3/C4 binding proteins.
Precision
Recovery
What's included?
Properties and storage information
Shipped at conditions
Appropriate short-term storage conditions
Appropriate long-term storage conditions
Storage information
Supplementary information
This supplementary information is collated from multiple sources and compiled automatically.
Biological function summary
CD62L is involved in the migration of leukocytes into lymphoid tissues and sites of inflammation. It is not known to be part of a larger protein complex; rather it functions independently on the cell membrane. CD62L also assists in the homing of naive lymphocytes to peripheral lymph nodes. The molecule regulates how immune cells enter tissues through high endothelial venules acting as a critical entry point for leukocyte trafficking.
Pathways
CD62L contributes significantly to the immune trafficking pathway and leukocyte adhesion cascade. It works closely with other adhesion molecules like CD44 and CD54 (ICAM-1) which are important within the context of cellular responses during inflammation. CD62L's function in these pathways ensures that immune cells are properly directed to areas where they are needed most whether that is secondary lymphoid organs or inflamed tissues.
Product protocols
- Visit the General protocols
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- Download websiteProtocolBooklet|en
Target data
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Product promise
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