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AB207925

Rat Lipocalin-2 ELISA Kit

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(6 Publications)

Rat Lipocalin-2 ELISA Kit is a Sandwich (quantitative) ELISA for the measurement of Rat Lipocalin-2 in Rat in Biofluids, Cell/Tissue Extracts, Cell Culture Media samples.

View Alternative Names

Neutrophil gelatinase-associated lipocalin, NGAL, Alpha-2-microglobulin-related protein, Alpha-2U globulin-related protein, Lipocalin 24p3, Lipocalin-2, Siderocalin LCN2, p25, Lcn2

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Sandwich ELISA - Rat Lipocalin-2 ELISA Kit (AB207925)
  • sELISA

Supplier Data

Sandwich ELISA - Rat Lipocalin-2 ELISA Kit (AB207925)

Abcam's Rat Lipocalin-2 ELISA Kit (ab207925) Calibration Curve

Key facts

Detection method

Colorimetric

Sample types

Urine, Plasma, Tissue Extracts, Serum, Tissue Culture Media

Reacts with

Rat

Assay type

Sandwich (quantitative)

Sensitivity

= 0.5 pg/mL

Range

4 - 400 pg/mL

Assay time

4h

Assay Platform

Pre-coated microplate (12 x 8 well strips)

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Product details

Abcam's Rat Lipocalin-2 ELISA Kit (ab207925) is an in vitro enzyme-linked immunosorbent assay (ELISA) for the quantitative measurement of Neutrophil Gelatinase-Associated Lipocalin levels in urine, plasma or serum, tissue extracts or culture media samples.

The assay is a sandwich ELISA performed in microwells coated with a mouse monoclonal antibody against Rat NGAL. Bound NGAL is detected with another mouse monoclonal antibody labeled with biotin and the assay is developed with horseradish peroxidase (HRP)-conjugated streptavidin and a color-forming substrate.

Please be aware this kit is supplied with a pre-coated 96-well plate that does not break into strips.

NGAL (neutrophil gelatinase-associated lipocalin) belongs to the lipocalin family of proteins. These are secreted proteins characterized by their ability to bind small hydrophobic molecules in a structurally conserved pocket formed by β-pleated sheet, to bind to specific cell-surface receptors, and to form macromolecular complexes. NGAL has many synonyms: perhaps the most widely used is lipocalin 2 (LCN 2); more recently the name siderocalin has been used to express NGAL's ability to bind bacterial siderophores. In the rat, NGAL was first named 25 kDa α2-microglobulin-related protein, and later neurelated lipocalin (NRL) because of its expression in mammary tumors initiated by the neu (HER2/c-erbB-2) oncogene. In the mouse, the homologous protein is known as oncogene protein 24p3, 24-kDa superinducible protein (SIP24) or uterocalin.

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Precision

[ { "reproducibilityType": "Inter", "sample": "Plasma", "replicates": 5, "mean": null, "standardDeviation": null, "coefficientOfVariability": "= 12" }, { "reproducibilityType": "Inter", "sample": "Urine", "replicates": 6, "mean": null, "standardDeviation": null, "coefficientOfVariability": "= 10" }, { "reproducibilityType": "Intra", "sample": "P1", "replicates": 8, "mean": null, "standardDeviation": null, "coefficientOfVariability": "= 3" }, { "reproducibilityType": "Intra", "sample": "P2", "replicates": 8, "mean": null, "standardDeviation": null, "coefficientOfVariability": "= 4" }, { "reproducibilityType": "Intra", "sample": "U1", "replicates": 8, "mean": null, "standardDeviation": null, "coefficientOfVariability": "= 3" }, { "reproducibilityType": "Intra", "sample": "U2", "replicates": 8, "mean": null, "standardDeviation": null, "coefficientOfVariability": "= 3" } ]
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Recovery

[ { "sample": "Urine", "range": "101 - 111 %", "average": "= 106" }, { "sample": "Plasma", "range": "99 - 108 %", "average": "= 104" } ]
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What's included?

{ "values": { "1x96Tests": { "sellingSize": "1 x 96 Tests", "publicAssetCode":"ab207925-1x96Tests", "assetComponentDetails": [ { "size":"1 x 96 Test", "name":"Microwell plate, 96 precoated wells", "number":"AB207925-CMP05", "productcode":"" }, { "size":"1 x 1 mL", "name":"Rat NGAL Standard (100 pg/mL)", "number":"AB207925-CMP11", "productcode":"" }, { "size":"1 x 40 mL", "name":"25x Wash Buffer Solution", "number":"AB207925-CMP01", "productcode":"" }, { "size":"1 x 1 mL", "name":"Rat NGAL Standard (4 pg/mL)", "number":"AB207925-CMP07", "productcode":"" }, { "size":"1 x 12 mL", "name":"TMB Substrate Solution", "number":"AB207925-CMP15", "productcode":"" }, { "size":"1 x 12 mL", "name":"Biotinylated Rat-NGAL Antibody", "number":"AB207925-CMP03", "productcode":"" }, { "size":"1 x 1 mL", "name":"Rat NGAL Standard (10 pg/mL)", "number":"AB207925-CMP08", "productcode":"" }, { "size":"1 x 12 mL", "name":"Stop Solution", "number":"AB207925-CMP14", "productcode":"" }, { "size":"1 x 1 mL", "name":"Rat NGAL Standard (400 pg/mL)", "number":"AB207925-CMP13", "productcode":"" }, { "size":"1 x 1 mL", "name":"Rat NGAL Standard (40 pg/mL)", "number":"AB207925-CMP10", "productcode":"" }, { "size":"1 x 12 mL", "name":"HRP-Streptavidin Solution", "number":"AB207925-CMP04", "productcode":"" }, { "size":"1 x 50 mL", "name":"5x Sample Diluent Buffer", "number":"AB207925-CMP02", "productcode":"" }, { "size":"1 x 1 mL", "name":"Rat NGAL Standard (0 pg/mL)", "number":"AB207925-CMP06", "productcode":"" }, { "size":"1 x 1 mL", "name":"Rat NGAL Standard (20 pg/mL)", "number":"AB207925-CMP09", "productcode":"" }, { "size":"1 x 1 mL", "name":"Rat NGAL Standard (200 pg/mL)", "number":"AB207925-CMP12", "productcode":"" } ] } } }
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Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
+4°C
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Product protocols

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Target data

Iron-trafficking protein involved in multiple processes such as apoptosis, innate immunity and renal development (By similarity). Binds iron through association with 2,3-dihydroxybenzoic acid (2,3-DHBA), a siderophore that shares structural similarities with bacterial enterobactin, and delivers or removes iron from the cell, depending on the context. Iron-bound form (holo-24p3) is internalized following binding to the SLC22A17 (24p3R) receptor, leading to release of iron and subsequent increase of intracellular iron concentration. In contrast, association of the iron-free form (apo-24p3) with the SLC22A17 (24p3R) receptor is followed by association with an intracellular siderophore, iron chelation and iron transfer to the extracellular medium, thereby reducing intracellular iron concentration. Involved in apoptosis due to interleukin-3 (IL3) deprivation : iron-loaded form increases intracellular iron concentration without promoting apoptosis, while iron-free form decreases intracellular iron levels, inducing expression of the proapoptotic protein BCL2L11/BIM, resulting in apoptosis (By similarity). Involved in innate immunity; limits bacterial proliferation by sequestering iron bound to microbial siderophores, such as enterobactin. Can also bind siderophores from M.tuberculosis (By similarity).
See full target information Lcn2
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Publications (6)

Recent publications for all applications. Explore the full list and refine your search

Scientific reports 14:22652 PubMed39349636

2024

8-Aminoguanine and its actions in the metabolic syndrome.

Applications

Unspecified application

Species

Unspecified reactive species

Edwin K Jackson,Delbert G Gillespie,Zaichuan Mi,Lori A Birder,Stevan P Tofovic

International journal of molecular sciences 25: PubMed38256015

2024

Oregano () Essential Oil and Its Constituents Prevent Rat Kidney Tissue Injury and Inflammation Induced by a High Dose of L-Arginine.

Applications

Unspecified application

Species

Unspecified reactive species

Nikola M Stojanović,Katarina V Mitić,Milica Nešić,Milica Stanković,Vladimir Petrović,Marko Baralić,Pavle J Randjelović,Dušan Sokolović,Niko Radulović

Clinical science (London, England : 1979) 136:675-694 PubMed35441670

2022

Differential effects of cyclo-oxygenase 1 and 2 inhibition on angiogenesis inhibitor-induced hypertension and kidney damage.

Applications

Unspecified application

Species

Unspecified reactive species

Katrina M Mirabito Colafella,Daan C H van Dorst,Rugina I Neuman,Leni van Doorn,Karla Bianca Neves,Augusto C Montezano,Ingrid M Garrelds,Richard van Veghel,René de Vries,Estrellita Uijl,Marian C Clahsen-van Groningen,Hans J Baelde,Anton H van den Meiracker,Rhian M Touyz,Willy Visser,A H Jan Danser,Jorie Versmissen

Journal of the American Heart Association 10:e020088 PubMed33682436

2021

Long-Term Dipeptidyl Peptidase 4 Inhibition Worsens Hypertension and Renal and Cardiac Abnormalities in Obese Spontaneously Hypertensive Heart Failure Rats.

Applications

Unspecified application

Species

Unspecified reactive species

Edwin K Jackson,Zaichuan Mi,Delbert G Gillespie,Dongmei Cheng,Stevan P Tofovic

Antioxidants (Basel, Switzerland) 8: PubMed31590249

2019

Anthocyanins Protect Hepatocytes against CCl-Induced Acute Liver Injury in Rats by Inhibiting Pro-inflammatory mediators, Polyamine Catabolism, Lipocalin-2, and Excessive Proliferation of Kupffer Cells.

Applications

Unspecified application

Species

Unspecified reactive species

Dejan Popović,Gordana Kocić,Vuka Katić,Aleksandra Zarubica,Ljubinka Janković Veličković,Vanja P Ničković,Andrija Jović,Andrej Veljković,Vladimir Petrović,Violeta Rakić,Zorica Jović,Nataša Poklar Ulrih,Danka Sokolović,Marko Stojanović,Marko Stanković,Goran Radenković,Gordana R Nikolić,Аzra Lukač,Aleksandar Milosavljević,Dušan Sokolović

Chemico-biological interactions 304:61-72 PubMed30825423

2019

Protective effects of anthocyanins from bilberry extract in rats exposed to nephrotoxic effects of carbon tetrachloride.

Applications

Unspecified application

Species

Unspecified reactive species

Dejan Popović,Gordana Kocić,Vuka Katić,Zorica Jović,Aleksandra Zarubica,Ljubinka Janković Veličković,Valentina Nikolić,Andrija Jović,Braca Kundalić,Violeta Rakić,Nataša Poklar Ulrih,Mihaela Skrt,Danka Sokolović,Ljubomir Dinić,Marko Stojanović,Aleksandar Milosavljević,Filip Veličković,Dušan Sokolović
View all publications
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