Rat Lipocalin-2 ELISA Kit (NGAL)

Product details

Rat Lipocalin-2 ELISA Kit (NGAL) ab119602 is a sandwich ELISA to measure Rat Lipocalin-2 (NGAL) in serum, plasma, cell culture supernatant, urine with a sensitivity of 10 pg/ml.

How the assay works

A Lipocalin-2 specific mouse monoclonal antibody for Lipocalin-2 has been precoated onto 96-well plates. Standards and test samples are added to the wells and incubated. A biotinylated detection polyclonal antibody from goat specific for Lipocalin-2 is then added followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex is added and unbound conjugates are washed away with PBS or TBS buffer. TMB is then used to visualize the HRP enzymatic reaction. TMB is catalyzed by HRP to produce a blue color product that changes into yellow after adding acidic stop solution. The density of yellow coloration is directly proportional to the rat Lipocalin-2 amount of sample captured in plate.

Assay Specificity

Our ELISA kits are rigorously validated to ensure the highest level of consistency and reproducibility. Please check the protocol booklet for more details

Rat Lipocalin-2 ELISA Kit (NGAL) ab119602 protocol summary

1. Add standard or sample to each well used. Incubate 37C
2. Add prepared biotin antibody to each well. Incubate at 37C
3. Add prepared Avidin-BiotinPeroxidase Complex (ABC). Incubate at 37C
4. Add TMB to each well. Incubate at room temperature.
5. Add Stop Solution to each well. Read at 450 nm

*Note: it is important to not use anticoagulants other than the ones described in the protocol to treat plasma, for other anticoagulants could block the antibody binding site.