Rat M-CSF ELISA Kit (CSF1)
- Recombinant
- SimpleStep
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(2 Publications)
Rat M-CSF ELISA Kit (CSF1) is a single-wash 90-min Simplestep used to quantify Rat M-CSF (CSF1) with a sensitivity of 6 pg/ml. The assay uses a simple mix-wash-read protocol with just one incubation and one wash step.
- Colorimetric Sandwich ELISA - 450 nm readout : works on any standard plate reader
- Design your own immunoassay: we also offer the conjugation-ready antibody pair
View Alternative Names
Csfm, Csf1, Macrophage colony-stimulating factor 1, CSF-1, MCSF, Proteoglycan macrophage colony-stimulating factor, PG-M-CSF
- sELISA
Unknown
Sandwich ELISA - Rat M-CSF ELISA Kit (CSF1) (AB253214)
Interpolated concentrations of native M-CSF in rat serum and plasma samples.
The concentrations of M-CSF were measured in duplicates, interpolated from the M-CSF standard curves and corrected for sample dilution. Undiluted samples are as follows : serum 30%, plasma (citrate) 30%, plasma (heparin) 40% and plasma (EDTA) 40% %. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean M-CSF concentration was determined to be 39,17 pg/mL in serum, 2,688 pg/mL, plasma (citrate), 2,433 pg/mL in plasma (EDTA) and 4,740 pg/mL in plasma (heparin).
- sELISA
Unknown
Sandwich ELISA - Rat M-CSF ELISA Kit (CSF1) (AB253214)
Example of rat M-CSF standard curve in Sample Diluent NS.
The M-CSF standard curve was prepared as described in Section 10. Raw data values are shown in the table. Background-subtracted data values (mean +/- SD) are graphed.
Reactivity data
Product details
Rat M-CSF ELISA Kit (CSF1) (ab253214) is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of M-CSF (CSF1) protein in cit plasma, edta plasma, hep plasma, and serum. It uses our proprietary SimpleStep ELISA® technology. Quantitate Rat M-CSF (CSF1) with 6 pg/ml sensitivity.
SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:
- Single-wash protocol reduces assay time to 90 minutes or less
- High sensitivity, specificity and reproducibility from superior antibodies
- Fully validated in biological samples
- 96-wells plate breakable into 12 x 8 wells strips
A 384-well SimpleStep ELISA® microplate (ab203359) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.
Macrophage colony-stimulating factor 1 (M-CSF), also known as CSF1, is a secreted cytokine that plays an essential role in the regulation of differentiation and maintenance of hematopoietic precursor cells, especially mononuclear phagocytes, such as macrophages and monocytes. It plays an important role in innate immunity and in inflammatory processes where it acts to promote the release of proinflammatory chemokines. M-CSF also plays an important role in the regulation of osteoclast proliferation and differentiation, the regulation of bone resorption, and is required for normal bone development. Reports show that it is required for normal male and female fertility. It also acts in reorganization of the actin cytoskeleton, regulates formation of membrane ruffles, cell adhesion and cell migration.
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Supplementary information
This supplementary information is collated from multiple sources and compiled automatically.
Biological function summary
M-CSF influences the production differentiation and function of macrophages and osteoclasts. It is not part of a large complex but interacts with its receptor the CSF1R (colony-stimulating factor 1 receptor) on the surface of target cells. This interaction promotes the survival and proliferation of the target cells playing significant roles in immune response and bone homeostasis by regulating osteoclast development.
Pathways
The interaction of M-CSF with its receptor is central to several biological pathways notably the immune system pathway and bone resorption pathway. Within these pathways the binding of M-CSF to CSF1R activates downstream signaling cascades such as the PI3K/AKT and MAPK pathways importantly affecting cell survival and differentiation. The M-CSF pathway intersects with other cytokines and factors like GM-CSF (granulocyte-macrophage colony-stimulating factor) which also regulate immune cell dynamics.
Product protocols
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Publications (2)
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International journal of molecular sciences 26: PubMed40244230
2025
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PLoS genetics 17:e1009605 PubMed34081701
2021
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