AB289902

Rat Macrophage Inflammatory Protein 3 alpha ELISA Kit

SimpleStep
What is this?

Be the first to review this product! Submit a review

(0 Publication)

Rat Macrophage Inflammatory Protein 3 alpha ELISA Kit is a single-wash 90-min Simplestep used to quantify Rat Macrophage Inflammatory Protein 3 alpha with a sensitivity of 1.571 pg/ml. The assay uses a simple mix-wash-read protocol with just one incubation and one wash step.

- Colorimetric Sandwich ELISA - 450 nm readout : works on any standard plate reader
- Design your own immunoassay: we also offer the conjugation-ready antibody pair

View Alternative Names

Scya20, St38, Ccl20, C-C motif chemokine 20, Beta-chemokine exodus-1, CC chemokine LARC, CC chemokine ST38, Liver and activation-regulated chemokine, Macrophage inflammatory protein 3 alpha, Small-inducible cytokine A20, MIP-3-alpha

3 Images

Sandwich ELISA - Rat Macrophage Inflammatory Protein 3 alpha ELISA Kit (AB289902)

sELISA

Unknown

Sandwich ELISA - Rat Macrophage Inflammatory Protein 3 alpha ELISA Kit (AB289902)

Interpolated concentrations of Macrophage Inflammatory Protein 3 alpha in rat spleen cell supernatants.

The concentration of Macrophage Inflammatory Protein 3 alpha was measured in neat rat spleen cell supernatants. The mean concentration was determined to be 105.63 pg/mL in Concanavalin A (5 mg/mL, 2 days) stimulated rat spleen supernatants and 4.92 pg/ml in unstimulated rat spleen supernatant.

sELISA

Unknown

Sandwich ELISA - Rat Macrophage Inflammatory Protein 3 alpha ELISA Kit (AB289902)

Example of rat Macrophage Inflammatory Protein 3 alpha standard curve in Sample Diluent NS.

Example of rat Macrophage Inflammatory Protein 3 alpha standard curve. Background-subtracted data values (mean +/- SD) are graphed.

sELISA

Unknown

Sandwich ELISA - Rat Macrophage Inflammatory Protein 3 alpha ELISA Kit (AB289902)

Interpolated concentrations of rat Macrophage Inflammatory Protein 3 alpha in stimulated spleen cell supernatant.

Interpolated concentration of native Macrophage Inflammatory Protein 3 alpha was measured in duplicate at different sample concentrations. Undiluted samples are neat spleen cell Concanavalin A (5 mg/mL, 2 days) stimulated supernatant. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). Sample dilutions are made in Sample Diluent NS.

Key facts

Detection method

Colorimetric

Sample types

Citrate plasma, Cell culture supernatant, EDTA Plasma

Reacts with

Rat

Assay type

Sandwich (quantitative)

Sensitivity

>= 1.571 pg/mL

Range

3.125 - 200 pg/mL

Assay time

1h 30m

Assay Platform

Pre-coated microplate (12 x 8 well strips)

style

left-column

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }

style

left-column

Product details

Rat Macrophage Inflammatory Protein 3 alpha ELISA Kit (ab289902) is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of Rat Macrophage Inflammatory Protein 3 alpha in cell culture supernatant and plasma samples. It uses our proprietary SimpleStep ELISA^® technology. Quantitate Rat Macrophage Inflammatory Protein 3 alpha with 1.571 pg/ml sensitivity. This kit is incompatible with serum and plasma (heparin) samples.

SimpleStep ELISA^® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA^® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA^® protocol summary in the image section for further details. Our SimpleStep ELISA^® technology provides several benefits:

-Single-wash protocol reduces assay time to 90 minutes or less
-High sensitivity, specificity and reproducibility from superior antibodies
-Fully validated in biological samples
-96-wells plate breakable into 12 x 8 wells strips

A 384-well SimpleStep ELISA® microplate (ab203359) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA^® kits.

style

left-column

Precision

[ { "reproducibilityType": "Inter", "sample": "Supernatant", "replicates": 4, "mean": null, "standardDeviation": null, "coefficientOfVariability": "6.2" }, { "reproducibilityType": "Intra", "sample": "Supernatant", "replicates": 8, "mean": null, "standardDeviation": null, "coefficientOfVariability": "4.9" } ]

style

left-column

Recovery

[ { "sample": "Cell culture supernatant", "range": "103 - 114 %", "average": "= 107" }, { "sample": "EDTA Plasma", "range": "111 - 115 %", "average": "= 113" }, { "sample": "Citrate plasma", "range": "89 - 95 %", "average": "= 93" } ]

style

left-column

What's included?

{ "values": { "1x96Tests": { "sellingSize": "1 x 96 Tests", "publicAssetCode":"ab289902-1x96Tests", "assetComponentDetails": [ { "size":"1 x 12 mL", "name":"Stop Solution", "number":"AB289902-CMP05", "productcode":"" }, { "size":"1 x 6 mL", "name":"Antibody Diluent CPR2", "number":"AB289902-CMP12", "productcode":"" }, { "size":"1 x 20 mL", "name":"10X Wash Buffer PT (ab206977)", "number":"AB289902-CMP10", "productcode":"" }, { "size":"1 x 12 mL", "name":"Sample Diluent NS (ab193972)", "number":"AB289902-CMP11", "productcode":"" }, { "size":"1 x 600 µL", "name":"10X Rat Macrophage Inflammatory Protein 3 alpha Capture Antibody", "number":"AB289902-CMP07", "productcode":"" }, { "size":"2 x 1 Vial", "name":"Rat Macrophage Inflammatory Protein 3 alpha Lyophilized Recombinant Protein", "number":"AB289902-CMP03", "productcode":"" }, { "size":"1 x 12 mL", "name":"TMB Development Solution", "number":"AB289902-CMP06", "productcode":"" }, { "size":"1 x 1 Unit", "name":"SimpleStep Pre-Coated 96-Well Microplate (ab206978)", "number":"AB289902-CMP04", "productcode":"" }, { "size":"1 x 600 µL", "name":"10X Rat Macrophage Inflammatory Protein 3 alpha Detector Antibody", "number":"AB289902-CMP08", "productcode":"" }, { "size":"1 x 1 Unit", "name":"Plate Seals", "number":"AB289902-CMP09", "productcode":"" } ] } } }

style

left-column

Properties and storage information

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

+4°C

Storage information

+4°C

style

left-column

Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Macrophage Inflammatory Protein 3 alpha (MIP-3 alpha) also known as CCL20 is a chemokine with a molecular mass of approximately 9 kDa. It plays a role in immune response by mediating chemotaxis of lymphocytes and dendritic cells. MIP-3 alpha is mainly expressed in liver lung and lymphoid organs including the lymph nodes and appendix. Its expression can be induced during inflammation in response to signaling molecules like tumor necrosis factor-alpha (TNF-alpha) and interleukin-1 beta (IL-1β).

Biological function summary

MIP-3 alpha contributes to the immune system by recruiting immune cells to sites of inflammation or injury. It is not part of a larger protein complex. Its main function is binding to the CCR6 receptor on target cells. This interaction mediates the migration and activation of the immune cells critical for initiating the immune response by increasing cell surface adhesion and motility. By drawing immune cells MIP-3 alpha significantly influences the body's capacity to fight infections and regulate inflammatory processes.

Pathways

MIP-3 alpha is integral to the inflammatory response and chemokine signaling pathways. It is involved in the immune system's adaptive response through its interaction with CCR6. This pathway overlaps with other chemokines such as MIP-1 and MIP-2 which also aid in immune cell recruitment. Furthermore MIP-3 alpha’s engagement in these pathways facilitates intercellular communication during immune responses pivotal for maintaining homeostasis and immune surveillance.

MIP-3 alpha has associations with inflammatory diseases like rheumatoid arthritis and inflammatory bowel disease. During these conditions elevated levels of MIP-3 alpha contribute to the recruitment of inflammatory cells which exacerbate symptoms. MIP-3 alpha also connects with other proteins such as TNF-alpha in these disease contexts enhancing inflammation and progression of these disorders. Targeting MIP-3 alpha and its associated pathways may provide therapeutic opportunities for alleviating these chronic inflammatory conditions.

style

left-column

Product protocols

style

left-column

Target data

Acts as a ligand for C-C chemokine receptor CCR6. Signals through binding and activation of CCR6 and induces a strong chemotactic response and mobilization of intracellular calcium ions. The ligand-receptor pair CCL20-CCR6 is responsible for the chemotaxis of dendritic cells (DC), effector/memory T-cells and B-cells and plays an important role at skin and mucosal surfaces under homeostatic and inflammatory conditions, as well as in pathology, including cancer and autoimmune diseases. CCL20 acts as a chemotactic factor that attracts lymphocytes and, slightly, neutrophils, but not monocytes. Involved in the recruitment of both the pro-inflammatory IL17 producing helper T-cells (Th17) and the regulatory T-cells (Treg) to sites of inflammation. Required for optimal migration of thymic natural regulatory T cells (nTregs) and DN1 early thymocyte progenitor cells. Positively regulates sperm motility and chemotaxis via its binding to CCR6 which triggers Ca2+ mobilization in the sperm which is important for its motility. May be involved in formation and function of the mucosal lymphoid tissues by attracting lymphocytes and dendritic cells towards epithelial cells.

See full target information Ccl20

style

left-column

style

left-column

websiteProtocolBooklet

style

right-column

Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Rat Macrophage Inflammatory Protein 3 alpha ELISA Kit

Sandwich ELISA - Rat Macrophage Inflammatory Protein 3 alpha ELISA Kit (AB289902)

Sandwich ELISA - Rat Macrophage Inflammatory Protein 3 alpha ELISA Kit (AB289902)

Sandwich ELISA - Rat Macrophage Inflammatory Protein 3 alpha ELISA Kit (AB289902)

Key facts

Detection method

Sample types

Reacts with

Assay type

Sensitivity

Range

Assay time

Assay Platform

Reactivity data

Product details

Precision

Recovery

What's included?

Properties and storage information

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Complementary Products

Assay Kits

Assay Kits

Primary Antibodies

Assay Kits

Product promise