AB219045

Rat MCP1 ELISA Kit

Recombinant
SimpleStep
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(11 Publications)

Rat MCP1 ELISA Kit is a single-wash 90-min Simplestep used to quantify Rat MCP1 with a sensitivity of 3.3 pg/ml. The assay uses a simple mix-wash-read protocol with just one incubation and one wash step.

- Colorimetric Sandwich ELISA - 450 nm readout : works on any standard plate reader
- Design your own immunoassay: we also offer the conjugation-ready antibody pair
- Cited in over 10 citations

View Alternative Names

Je, Mcp1, Scya2, Ccl2, C-C motif chemokine 2, Immediate-early serum-responsive protein JE, Monocyte chemoattractant protein 1, Monocyte chemotactic protein 1, Small-inducible cytokine A2, MCP-1

3 Images

ELISA

Supplier Data

ELISA - Rat MCP1 ELISA Kit (AB219045)

sELISA

Supplier Data

Sandwich ELISA - Rat MCP1 ELISA Kit (AB219045)

Interpolated concentrations of native MCP1 in rat serum, plasma and cell culture supernatant samples.

The concentrations of MCP1 were measured in duplicates, interpolated from the MCP1 standard curves and corrected for sample dilution. Undiluted samples are as follows : serum 7.5%, plasma (citrate) 50% and spleen supernatant 2%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The MCP1 concentration was determined to be 5,485 pg/mL in serum, 1,615 pg/mL in plasma (citrate) and 40,501 pg/mL in and spleen supernatant.

sELISA

Supplier Data

Sandwich ELISA - Rat MCP1 ELISA Kit (AB219045)

Example of rat MCP1 standard curve in Sample Diluent NS.

Background-subtracted data values (mean +/- SD) are graphed.

Key facts

Detection method

Colorimetric

Sample types

Cell culture supernatant, Citrate plasma, EDTA Plasma, Heparin Plasma, Serum

Reacts with

Rat

Assay type

Sandwich (quantitative)

Sensitivity

= 3.3 pg/mL

Range

25 - 1600 pg/mL

Assay time

1h 30m

Assay Platform

Pre-coated microplate (12 x 8 well strips)

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }

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Product details

Rat MCP1 ELISA Kit ab219045 is a rapid single-wash 90-min Sandwich ELISA to measure Rat MCP1 in cell culture supernatant, citrate plasma, EDTA plasma, heparin plasma, serum. This SimpleStep sensitivity is 3.3 pg/mL.

How the assay works

Rat MCP1 SimpleStep ELISA^®employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA^® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA^® protocol summary in the image section for further details.

Assay Specificity

Our SimpleStep ELISA^® kits use recombinant monoclonal antibodies rigorously validated to ensure the highest level of consistency and reproducibility, improved sensitivity and specificity and ease of scalability and security of supply.
Please refer to our protocol booklet for more details.

Rat MCP1 ELISA Kit ab219045 protocol summary

1. Mix: add samples/standards to the wells together with the capture and detector antibody cocktail. Incubate 1 hr at room temperature
2. Wash
3. Add TMB development solution - incubate for 10 min
4. Add Stop solution
5. Read the results on a plate reader at 450 nm

Design your own immunoassay

We offer the antibody pair used in this kit in a BSA and Azide-free format, ready for conjugation:

- Anti-MCP1 antibody [EPR19906-201] - BSA and Azide free (Capture) ab242910
- Anti-MCP1 antibody [EPR19906-218] - BSA and Azide free (Detector) ab242910

MCP-1 (CCL2) is a chemokine expressed by fibroblasts, macrophages, endothelial cells, mast cells, osteoblasts, ameloblasts, epithelial cells, and astrocytes after stimulation with inflammatory stimuli. MCP-1 is involved in the recruitment of monocytes and lymphocytes and in the regulation of Th1/Th2 lymphocyte differentiation.

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Precision

[ { "reproducibilityType": "Inter", "sample": "Overall", "replicates": 3, "mean": null, "standardDeviation": null, "coefficientOfVariability": "6.5" }, { "reproducibilityType": "Intra", "sample": "Overall", "replicates": 5, "mean": null, "standardDeviation": null, "coefficientOfVariability": "3.5" } ]

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Recovery

[ { "sample": "Cell culture supernatant", "range": "88 - 98 %", "average": "= 93" }, { "sample": "Serum", "range": "109 - 113 %", "average": "= 111" }, { "sample": "Citrate plasma", "range": "94 - 107 %", "average": "= 101" } ]

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What's included?

{ "values": { "1x96Tests": { "sellingSize": "1 x 96 Tests", "publicAssetCode":"ab219045-1x96Tests", "assetComponentDetails": [ { "size":"1 x 12 mL", "name":"Stop Solution", "number":"AB219045-CMP07", "productcode":"" }, { "size":"1 x 50 mL", "name":"Sample Diluent NS (ab193972)", "number":"AB219045-CMP05", "productcode":"" }, { "size":"1 x 20 mL", "name":"10X Wash Buffer PT (ab206977)", "number":"AB219045-CMP10", "productcode":"" }, { "size":"1 x 6 mL", "name":"Antibody Diluent 5BR", "number":"AB219045-CMP01", "productcode":"" }, { "size":"1 x 1 Unit", "name":"Plate Seals", "number":"AB219045-CMP09", "productcode":"" }, { "size":"1 x 12 mL", "name":"TMB Development Solution", "number":"AB219045-CMP08", "productcode":"" }, { "size":"1 x 1 Unit", "name":"SimpleStep Pre-Coated 96-Well Microplate (ab206978)", "number":"AB219045-CMP06", "productcode":"" }, { "size":"2 x 1 Vial", "name":"Rat MCP1 Lyophilized Recombinant Protein", "number":"AB219045-CMP04", "productcode":"" }, { "size":"1 x 600 µL", "name":"10X Rat MCP1 Capture Antibody", "number":"AB219045-CMP02", "productcode":"" }, { "size":"1 x 1 Vial", "name":"Rat MCP1 Detector Antibody (Lyophilized)", "number":"AB219045-CMP03", "productcode":"" } ] } } }

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Properties and storage information

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

+4°C

Storage information

+4°C

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

MCP-1 also known as CCL2 is a chemokine involved in the recruitment of monocytes to sites of injury and inflammation. This protein consists of 76 amino acids and has a molecular weight of about 11 kDa. MCP-1 is expressed in a wide range of cell types including endothelial cells fibroblasts and monocytes. It acts by binding to the chemokine receptor CCR2 stimulating intracellular signaling pathways that mediate cell migration.

Biological function summary

The role of MCP-1 is significant in guiding immune cells to areas requiring immune response. It does not form part of a complex but operates as a singular entity to affect immune signaling. MCP-1 influences the behavior of monocytes and macrophages by inducing chemotaxis and activating their effector functions. Through this mechanism it aids in the inflammatory response and tissue repair.

Pathways

MCP-1 participates in the inflammatory pathways by cooperating with key proteins like CCR2. It facilitates the JAK-STAT signaling pathway which is vital for transmitting extracellular information into cellular responses. The interaction of MCP-1 with CCR2 influences the activation of downstream proteins like STAT3 enabling various immune responses. MCP-1 thereby plays a central role in modulating inflammation-related pathways.

MCP-1 has a connection to inflammatory bowel disease and atherosclerosis. In such conditions elevated levels of MCP-1 can exacerbate inflammatory responses by recruiting monocytes to dysfunctional sites worsening tissue damage. Through its interaction with CCR2 MCP-1 becomes a pivotal factor in chronic inflammation. Targeting this MCP-1/CCR2 axis can provide therapeutic opportunities for modulating disease progression in these inflammatory diseases.

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Product protocols

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Target data

Acts as a ligand for C-C chemokine receptor CCR2 (By similarity). Signals through binding and activation of CCR2 and induces a strong chemotactic response and mobilization of intracellular calcium ions (By similarity). Exhibits a chemotactic activity for monocytes and basophils but not neutrophils or eosinophils (By similarity). Plays an important role in mediating peripheral nerve injury-induced neuropathic pain (By similarity). Increases NMDA-mediated synaptic transmission in both dopamine D1 and D2 receptor-containing neurons, which may be caused by MAPK/ERK-dependent phosphorylation of GRIN2B/NMDAR2B (By similarity).

See full target information Ccl2

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Publications (11)

Recent publications for all applications. Explore the full list and refine your search

Cells 13: PubMed39056770

2024

Octadecaneuropeptide, ODN, Promotes Cell Survival against 6-OHDA-Induced Oxidative Stress and Apoptosis by Modulating the Expression of miR-34b, miR-29a, and miR-21in Cultured Astrocytes.

Applications

Unspecified application

Species

Unspecified reactive species

Amine Bourzam,Yosra Hamdi,Seyma Bahdoudi,Karthi Duraisamy,Mouna El Mehdi,Magali Basille-Dugay,Omayma Dlimi,Maher Kharrat,Anne Vejux,Gérard Lizard,Taoufik Ghrairi,Benjamin Lefranc,David Vaudry,Jean A Boutin,Jérôme Leprince,Olfa Masmoudi-Kouki

Frontiers in molecular neuroscience 16:1261217 PubMed38379852

2024

Exploring gene signatures and regulatory networks in a rat model of sciatica: implications and validation in neuropathic pain.

Applications

Unspecified application

Species

Unspecified reactive species

Mu Xu,Zhijian Wang,Gang Xu,Mengye Zhu,Daying Zhang,Yi Yan

Chemical biology & drug design 103:e14386 PubMed37923393

2023

Protective effects of baicalin on diethyl nitrosamine-induced liver cirrhosis by suppressing oxidative stress and inflammation.

Applications

Unspecified application

Species

Unspecified reactive species

Hui Wang,Yufei Chang,Xiao Liu,Linan Liu,Mingxi Hua,Ang Li

Regenerative therapy 22:210-223 PubMed36926469

2023

Therapeutic role of adipose-derived mesenchymal stem cells-derived extracellular vesicles in rats with obstructive sleep apnea hypopnea syndrome.

Applications

Unspecified application

Species

Unspecified reactive species

Lin Xu,Lu Zhang,Yang Xiang,Xiangyan Zhang

Antioxidants (Basel, Switzerland) 11: PubMed36009287

2022

Renal Ischemia/Reperfusion Mitigation via Geraniol: The Role of Nrf-2/HO-1/NQO-1 and TLR2,4/MYD88/NFκB Pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Maged E Mohamed,Mohammad A Elmorsy,Nancy S Younis

Antioxidants (Basel, Switzerland) 11: PubMed35326185

2022

The Protective Effect of Anethole against Renal Ischemia/Reperfusion: The Role of the TLR2,4/MYD88/NFκB Pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Maged Elsayed Mohamed,Mahmoud Kandeel,Hany M Abd El-Lateef,Hossam S El-Beltagi,Nancy S Younis

Experimental and therapeutic medicine 21:467 PubMed33763154

2021

Identification of miR-145 as a regulator of the cardiomyocyte inflammatory response and oxidative stress under hyperglycemia.

Applications

Unspecified application

Species

Unspecified reactive species

Wan Zheng,Tianfa Li,Junping Wei,Yuanyuan Zhang,Qi Zuo,Yun Lin

Molecular medicine reports 23: PubMed33300078

2020

Anti‑inflammatory effect of salusin‑β knockdown on LPS‑activated alveolar macrophages via NF‑κB inhibition and HO‑1 activation.

Applications

Unspecified application

Species

Unspecified reactive species

Sheng Chen,Yunnan Hu,Jiaxin Zhang,Pengyu Zhang

International journal of hypertension 2020:6250425 PubMed33014451

2020

Magnesium Lithospermate B Downregulates the Levels of Blood Pressure, Inflammation, and Oxidative Stress in Pregnant Rats with Hypertension.

Applications

Unspecified application

Species

Unspecified reactive species

Kaixiang Xu,Xiaohong Zang,Mian Peng,Qian Zhao,Binbin Lin

Medical science monitor : international medical jo 25:9346-9356 PubMed31812978

2019

Spleen Tyrosine Kinase (SYK) in the Progression of Peritoneal Fibrosis Through Activation of the TGF-β1/Smad3 Signaling Pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Kang-Han Liu,Nan Zhou,Yan Zou,Yi-Ya Yang,Sha-Xi OuYang,Yu-Mei Liang

View all publications

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websiteProtocolBooklet

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Rat MCP1 ELISA Kit

ELISA - Rat MCP1 ELISA Kit (AB219045)

Sandwich ELISA - Rat MCP1 ELISA Kit (AB219045)

Sandwich ELISA - Rat MCP1 ELISA Kit (AB219045)

Key facts

Detection method

Sample types

Reacts with

Assay type

Sensitivity

Range

Assay time

Assay Platform

Reactivity data

Product details

Precision

Recovery

What's included?

Properties and storage information

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (11)

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